Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06765 and EX31_RS06770 overlap by 4 nucleotides EX31_RS06770 and EX31_RS06775 are separated by 76 nucleotides
EX31_RS06765: EX31_RS06765 - histidinol-phosphate transaminase, at 139,416 to 140,582
_RS06765
EX31_RS06770: EX31_RS06770 - alpha-hydroxy-acid oxidizing protein, at 140,579 to 141,730
_RS06770
EX31_RS06775: EX31_RS06775 - iron-containing alcohol dehydrogenase family protein, at 141,807 to 142,892
_RS06775
Position (kb) 140
141
142 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 139.584 kb on - strand, within EX31_RS06765 at 139.627 kb on + strand, within EX31_RS06765 at 139.657 kb on - strand, within EX31_RS06765 at 139.724 kb on + strand, within EX31_RS06765 at 139.725 kb on - strand, within EX31_RS06765 at 139.725 kb on - strand, within EX31_RS06765 at 140.053 kb on - strand, within EX31_RS06765 at 140.083 kb on - strand, within EX31_RS06765 at 140.448 kb on - strand, within EX31_RS06765 at 140.452 kb on - strand, within EX31_RS06765 at 140.487 kb on + strand at 140.540 kb on + strand at 140.558 kb on + strand at 140.650 kb on + strand at 140.654 kb on + strand at 140.654 kb on + strand at 140.654 kb on + strand at 140.654 kb on + strand at 140.654 kb on + strand at 140.654 kb on + strand at 140.655 kb on - strand at 140.655 kb on - strand at 140.655 kb on - strand at 140.655 kb on - strand at 140.803 kb on - strand, within EX31_RS06770 at 140.969 kb on + strand, within EX31_RS06770 at 140.969 kb on + strand, within EX31_RS06770 at 140.969 kb on + strand, within EX31_RS06770 at 140.969 kb on + strand, within EX31_RS06770 at 140.969 kb on + strand, within EX31_RS06770 at 140.969 kb on + strand, within EX31_RS06770 at 140.970 kb on - strand, within EX31_RS06770 at 141.085 kb on + strand, within EX31_RS06770 at 141.085 kb on + strand, within EX31_RS06770 at 141.091 kb on + strand, within EX31_RS06770 at 141.091 kb on + strand, within EX31_RS06770 at 141.171 kb on + strand, within EX31_RS06770 at 141.213 kb on - strand, within EX31_RS06770 at 141.226 kb on + strand, within EX31_RS06770 at 141.585 kb on + strand, within EX31_RS06770 at 141.585 kb on + strand, within EX31_RS06770 at 141.585 kb on + strand, within EX31_RS06770 at 141.586 kb on - strand, within EX31_RS06770 at 141.586 kb on - strand, within EX31_RS06770 at 141.586 kb on - strand, within EX31_RS06770 at 141.586 kb on - strand, within EX31_RS06770 at 141.586 kb on - strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.587 kb on + strand, within EX31_RS06770 at 141.588 kb on - strand, within EX31_RS06770 at 141.588 kb on - strand, within EX31_RS06770 at 141.738 kb on + strand at 141.739 kb on - strand at 141.806 kb on + strand at 141.821 kb on + strand at 141.821 kb on + strand at 141.822 kb on - strand at 141.824 kb on + strand at 141.861 kb on - strand at 142.106 kb on + strand, within EX31_RS06775 at 142.107 kb on - strand, within EX31_RS06775 at 142.231 kb on + strand, within EX31_RS06775 at 142.422 kb on + strand, within EX31_RS06775 at 142.502 kb on - strand, within EX31_RS06775 at 142.511 kb on + strand, within EX31_RS06775 at 142.581 kb on + strand, within EX31_RS06775 at 142.581 kb on + strand, within EX31_RS06775 at 142.581 kb on + strand, within EX31_RS06775 at 142.582 kb on - strand, within EX31_RS06775
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 139,584 - EX31_RS06765 0.14 -0.4 139,627 + EX31_RS06765 0.18 +1.9 139,657 - EX31_RS06765 0.21 +0.9 139,724 + EX31_RS06765 0.26 -1.0 139,725 - EX31_RS06765 0.26 -0.3 139,725 - EX31_RS06765 0.26 -0.4 140,053 - EX31_RS06765 0.55 -2.4 140,083 - EX31_RS06765 0.57 +0.3 140,448 - EX31_RS06765 0.88 +1.5 140,452 - EX31_RS06765 0.89 -0.5 140,487 + -0.2 140,540 + +0.8 140,558 + -1.6 140,650 + +0.1 140,654 + +0.7 140,654 + -1.1 140,654 + -0.2 140,654 + +0.0 140,654 + +1.9 140,654 + -0.3 140,655 - +1.7 140,655 - -0.1 140,655 - +1.0 140,655 - +1.3 140,803 - EX31_RS06770 0.19 +0.6 140,969 + EX31_RS06770 0.34 +1.0 140,969 + EX31_RS06770 0.34 -1.2 140,969 + EX31_RS06770 0.34 -0.1 140,969 + EX31_RS06770 0.34 +2.3 140,969 + EX31_RS06770 0.34 -0.4 140,969 + EX31_RS06770 0.34 +0.9 140,970 - EX31_RS06770 0.34 -0.3 141,085 + EX31_RS06770 0.44 +1.3 141,085 + EX31_RS06770 0.44 -0.4 141,091 + EX31_RS06770 0.44 +0.5 141,091 + EX31_RS06770 0.44 -2.3 141,171 + EX31_RS06770 0.51 -0.7 141,213 - EX31_RS06770 0.55 +2.2 141,226 + EX31_RS06770 0.56 -0.7 141,585 + EX31_RS06770 0.87 -0.9 141,585 + EX31_RS06770 0.87 +0.3 141,585 + EX31_RS06770 0.87 +3.5 141,586 - EX31_RS06770 0.87 -1.1 141,586 - EX31_RS06770 0.87 -1.4 141,586 - EX31_RS06770 0.87 +0.2 141,586 - EX31_RS06770 0.87 +1.6 141,586 - EX31_RS06770 0.87 -0.7 141,587 + EX31_RS06770 0.88 -1.4 141,587 + EX31_RS06770 0.88 -0.1 141,587 + EX31_RS06770 0.88 +0.3 141,587 + EX31_RS06770 0.88 -0.4 141,587 + EX31_RS06770 0.88 +1.3 141,587 + EX31_RS06770 0.88 -0.9 141,587 + EX31_RS06770 0.88 +0.3 141,588 - EX31_RS06770 0.88 +0.4 141,588 - EX31_RS06770 0.88 -0.4 141,738 + +0.2 141,739 - +0.9 141,806 + +0.1 141,821 + +0.0 141,821 + +1.0 141,822 - +1.5 141,824 + +0.9 141,861 - +0.2 142,106 + EX31_RS06775 0.28 +0.4 142,107 - EX31_RS06775 0.28 -0.4 142,231 + EX31_RS06775 0.39 -1.4 142,422 + EX31_RS06775 0.57 -2.3 142,502 - EX31_RS06775 0.64 +0.5 142,511 + EX31_RS06775 0.65 +0.5 142,581 + EX31_RS06775 0.71 +0.3 142,581 + EX31_RS06775 0.71 +0.7 142,581 + EX31_RS06775 0.71 +0.7 142,582 - EX31_RS06775 0.71 -0.4
Or see this region's nucleotide sequence