Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01105 and EX31_RS01110 are separated by 943 nucleotides EX31_RS01110 and EX31_RS01115 are separated by 70 nucleotides
EX31_RS01105: EX31_RS01105 - tryptophan permease, at 239,248 to 240,492
_RS01105
EX31_RS01110: EX31_RS01110 - hypothetical protein, at 241,436 to 242,170
_RS01110
EX31_RS01115: EX31_RS01115 - ABC-F family ATP-binding cassette domain-containing protein, at 242,241 to 243,974
_RS01115
Position (kb)
241
242
243 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 240.455 kb on - strand at 240.492 kb on + strand at 240.530 kb on - strand at 240.584 kb on - strand at 240.614 kb on + strand at 240.614 kb on + strand at 240.614 kb on + strand at 240.614 kb on + strand at 240.615 kb on - strand at 240.615 kb on - strand at 240.615 kb on - strand at 240.669 kb on + strand at 240.669 kb on + strand at 240.744 kb on + strand at 240.864 kb on + strand at 240.865 kb on - strand at 240.901 kb on + strand at 240.940 kb on + strand at 240.940 kb on + strand at 240.940 kb on + strand at 240.967 kb on + strand at 240.972 kb on + strand at 241.004 kb on + strand at 241.093 kb on + strand at 241.125 kb on + strand at 241.177 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.197 kb on + strand at 241.226 kb on + strand at 241.226 kb on + strand at 241.266 kb on + strand at 241.312 kb on + strand at 241.312 kb on + strand at 241.333 kb on + strand at 241.333 kb on + strand at 241.359 kb on + strand at 241.374 kb on + strand at 241.382 kb on + strand at 241.438 kb on - strand at 241.611 kb on + strand, within EX31_RS01110 at 241.611 kb on + strand, within EX31_RS01110 at 241.611 kb on + strand, within EX31_RS01110 at 241.612 kb on - strand, within EX31_RS01110 at 241.612 kb on - strand, within EX31_RS01110 at 241.657 kb on + strand, within EX31_RS01110 at 241.681 kb on - strand, within EX31_RS01110 at 241.686 kb on + strand, within EX31_RS01110 at 241.686 kb on + strand, within EX31_RS01110 at 241.687 kb on - strand, within EX31_RS01110 at 241.687 kb on - strand, within EX31_RS01110 at 241.688 kb on + strand, within EX31_RS01110 at 241.689 kb on - strand, within EX31_RS01110 at 241.689 kb on - strand, within EX31_RS01110 at 241.689 kb on - strand, within EX31_RS01110 at 241.969 kb on + strand, within EX31_RS01110 at 241.969 kb on + strand, within EX31_RS01110 at 241.969 kb on + strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.993 kb on + strand, within EX31_RS01110 at 241.993 kb on + strand, within EX31_RS01110 at 242.203 kb on + strand at 242.203 kb on + strand at 242.208 kb on - strand at 242.218 kb on - strand at 242.220 kb on - strand at 242.230 kb on - strand at 242.234 kb on + strand at 242.234 kb on + strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.242 kb on + strand at 242.243 kb on - strand at 242.243 kb on - strand at 242.474 kb on - strand, within EX31_RS01115 at 242.474 kb on - strand, within EX31_RS01115 at 242.658 kb on + strand, within EX31_RS01115 at 242.671 kb on + strand, within EX31_RS01115 at 242.672 kb on - strand, within EX31_RS01115 at 242.755 kb on + strand, within EX31_RS01115 at 242.872 kb on + strand, within EX31_RS01115 at 242.876 kb on - strand, within EX31_RS01115 at 242.933 kb on + strand, within EX31_RS01115
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 240,455 - +0.7 240,492 + +1.0 240,530 - -0.4 240,584 - +1.7 240,614 + +2.2 240,614 + +2.2 240,614 + -0.6 240,614 + +2.2 240,615 - -1.0 240,615 - +0.9 240,615 - -0.8 240,669 + +0.5 240,669 + -1.0 240,744 + -0.4 240,864 + -0.8 240,865 - +0.6 240,901 + -0.7 240,940 + +1.9 240,940 + -0.3 240,940 + +1.1 240,967 + -1.0 240,972 + -0.1 241,004 + +0.0 241,093 + +0.5 241,125 + +1.3 241,177 + -0.7 241,179 + +0.4 241,179 + -0.3 241,179 + -0.0 241,179 + -0.1 241,179 + -0.3 241,197 + -0.2 241,226 + -0.6 241,226 + -1.6 241,266 + -0.4 241,312 + -1.0 241,312 + -0.8 241,333 + -0.4 241,333 + -1.9 241,359 + +0.2 241,374 + +0.0 241,382 + +0.2 241,438 - +0.2 241,611 + EX31_RS01110 0.24 -0.1 241,611 + EX31_RS01110 0.24 +0.5 241,611 + EX31_RS01110 0.24 +1.0 241,612 - EX31_RS01110 0.24 -0.1 241,612 - EX31_RS01110 0.24 -0.8 241,657 + EX31_RS01110 0.30 +0.3 241,681 - EX31_RS01110 0.33 +1.7 241,686 + EX31_RS01110 0.34 -2.3 241,686 + EX31_RS01110 0.34 -0.8 241,687 - EX31_RS01110 0.34 -0.6 241,687 - EX31_RS01110 0.34 +0.4 241,688 + EX31_RS01110 0.34 +0.0 241,689 - EX31_RS01110 0.34 -2.0 241,689 - EX31_RS01110 0.34 -0.2 241,689 - EX31_RS01110 0.34 -0.3 241,969 + EX31_RS01110 0.73 +1.5 241,969 + EX31_RS01110 0.73 +0.2 241,969 + EX31_RS01110 0.73 -0.3 241,970 - EX31_RS01110 0.73 -0.7 241,970 - EX31_RS01110 0.73 +1.0 241,970 - EX31_RS01110 0.73 +0.6 241,970 - EX31_RS01110 0.73 -0.2 241,970 - EX31_RS01110 0.73 -0.6 241,971 + EX31_RS01110 0.73 +0.8 241,971 + EX31_RS01110 0.73 -0.0 241,971 + EX31_RS01110 0.73 +2.0 241,971 + EX31_RS01110 0.73 +0.5 241,971 + EX31_RS01110 0.73 -0.8 241,971 + EX31_RS01110 0.73 -1.8 241,972 - EX31_RS01110 0.73 +0.5 241,972 - EX31_RS01110 0.73 -0.4 241,972 - EX31_RS01110 0.73 +0.2 241,972 - EX31_RS01110 0.73 +2.0 241,993 + EX31_RS01110 0.76 -1.6 241,993 + EX31_RS01110 0.76 +0.2 242,203 + +1.1 242,203 + -1.5 242,208 - +1.3 242,218 - +0.8 242,220 - +1.7 242,230 - +0.2 242,234 + +0.2 242,234 + -0.6 242,235 - -0.3 242,235 - -0.3 242,235 - +0.2 242,235 - -0.1 242,235 - +0.9 242,235 - +1.8 242,235 - -0.2 242,242 + -0.1 242,243 - -1.1 242,243 - +0.7 242,474 - EX31_RS01115 0.13 +1.0 242,474 - EX31_RS01115 0.13 -1.1 242,658 + EX31_RS01115 0.24 -0.4 242,671 + EX31_RS01115 0.25 -0.1 242,672 - EX31_RS01115 0.25 +1.2 242,755 + EX31_RS01115 0.30 -0.4 242,872 + EX31_RS01115 0.36 +1.3 242,876 - EX31_RS01115 0.37 -0.1 242,933 + EX31_RS01115 0.40 -1.4
Or see this region's nucleotide sequence