Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01070 and EX31_RS01075 are separated by 259 nucleotides EX31_RS01075 and EX31_RS01080 are separated by 187 nucleotides EX31_RS01080 and EX31_RS01085 overlap by 4 nucleotides EX31_RS01085 and EX31_RS01090 overlap by 4 nucleotides
EX31_RS01070: EX31_RS01070 - hypothetical protein, at 234,105 to 234,452
_RS01070
EX31_RS01075: EX31_RS01075 - manganese-binding transcriptional regulator MntR, at 234,712 to 235,155
_RS01075
EX31_RS01080: EX31_RS01080 - metal ABC transporter substrate-binding protein, at 235,343 to 236,284
_RS01080
EX31_RS01085: EX31_RS01085 - ATP-binding cassette domain-containing protein, at 236,281 to 237,060
_RS01085
EX31_RS01090: EX31_RS01090 - metal ABC transporter permease, at 237,057 to 237,911
_RS01090
Position (kb)
235
236
237 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 234.346 kb on + strand, within EX31_RS01070 at 234.346 kb on + strand, within EX31_RS01070 at 234.347 kb on - strand, within EX31_RS01070 at 234.349 kb on + strand, within EX31_RS01070 at 234.361 kb on + strand, within EX31_RS01070 at 234.362 kb on - strand, within EX31_RS01070 at 234.379 kb on + strand, within EX31_RS01070 at 234.380 kb on - strand, within EX31_RS01070 at 234.452 kb on + strand at 234.453 kb on - strand at 234.493 kb on + strand at 234.493 kb on + strand at 234.494 kb on - strand at 234.522 kb on - strand at 234.708 kb on + strand at 234.828 kb on + strand, within EX31_RS01075 at 234.841 kb on + strand, within EX31_RS01075 at 234.842 kb on - strand, within EX31_RS01075 at 234.916 kb on + strand, within EX31_RS01075 at 234.970 kb on - strand, within EX31_RS01075 at 235.034 kb on - strand, within EX31_RS01075 at 235.034 kb on - strand, within EX31_RS01075 at 235.034 kb on - strand, within EX31_RS01075 at 235.034 kb on - strand, within EX31_RS01075 at 235.034 kb on - strand, within EX31_RS01075 at 235.034 kb on - strand, within EX31_RS01075 at 235.056 kb on - strand, within EX31_RS01075 at 235.056 kb on - strand, within EX31_RS01075 at 235.056 kb on - strand, within EX31_RS01075 at 235.056 kb on - strand, within EX31_RS01075 at 235.067 kb on - strand, within EX31_RS01075 at 235.067 kb on - strand, within EX31_RS01075 at 235.097 kb on - strand, within EX31_RS01075 at 235.130 kb on - strand at 235.157 kb on - strand at 235.187 kb on + strand at 235.200 kb on + strand at 235.201 kb on - strand at 235.224 kb on + strand at 235.224 kb on + strand at 235.224 kb on + strand at 235.225 kb on - strand at 235.233 kb on + strand at 235.233 kb on + strand at 235.233 kb on + strand at 235.233 kb on + strand at 235.234 kb on - strand at 235.234 kb on - strand at 235.234 kb on - strand at 235.234 kb on - strand at 235.234 kb on - strand at 235.244 kb on - strand at 235.280 kb on + strand at 235.290 kb on + strand at 235.292 kb on + strand at 235.292 kb on + strand at 235.303 kb on + strand at 235.371 kb on + strand at 235.379 kb on + strand at 235.543 kb on - strand, within EX31_RS01080 at 235.645 kb on + strand, within EX31_RS01080 at 235.732 kb on + strand, within EX31_RS01080 at 235.748 kb on + strand, within EX31_RS01080 at 235.748 kb on + strand, within EX31_RS01080 at 235.748 kb on + strand, within EX31_RS01080 at 235.748 kb on + strand, within EX31_RS01080 at 235.748 kb on + strand, within EX31_RS01080 at 235.749 kb on - strand, within EX31_RS01080 at 235.802 kb on + strand, within EX31_RS01080 at 235.802 kb on + strand, within EX31_RS01080 at 235.835 kb on + strand, within EX31_RS01080 at 235.860 kb on - strand, within EX31_RS01080 at 235.874 kb on - strand, within EX31_RS01080 at 235.982 kb on + strand, within EX31_RS01080 at 235.997 kb on + strand, within EX31_RS01080 at 236.015 kb on + strand, within EX31_RS01080 at 236.015 kb on + strand, within EX31_RS01080 at 236.015 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.228 kb on + strand at 236.228 kb on + strand at 236.228 kb on + strand at 236.228 kb on + strand at 236.229 kb on - strand at 236.316 kb on + strand at 236.316 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.418 kb on + strand, within EX31_RS01085 at 236.433 kb on + strand, within EX31_RS01085 at 236.433 kb on + strand, within EX31_RS01085 at 236.527 kb on + strand, within EX31_RS01085 at 236.527 kb on + strand, within EX31_RS01085 at 236.528 kb on - strand, within EX31_RS01085 at 236.641 kb on - strand, within EX31_RS01085 at 236.965 kb on - strand, within EX31_RS01085 at 237.012 kb on + strand at 237.012 kb on + strand at 237.088 kb on - strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.094 kb on - strand at 237.095 kb on + strand at 237.095 kb on + strand at 237.096 kb on - strand at 237.276 kb on - strand, within EX31_RS01090
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 234,346 + EX31_RS01070 0.69 -0.4 234,346 + EX31_RS01070 0.69 +0.3 234,347 - EX31_RS01070 0.70 -1.4 234,349 + EX31_RS01070 0.70 +1.6 234,361 + EX31_RS01070 0.74 -1.6 234,362 - EX31_RS01070 0.74 +0.2 234,379 + EX31_RS01070 0.79 +0.1 234,380 - EX31_RS01070 0.79 +1.4 234,452 + -1.2 234,453 - -0.2 234,493 + +0.5 234,493 + +0.3 234,494 - +2.5 234,522 - -1.7 234,708 + -0.1 234,828 + EX31_RS01075 0.26 +0.1 234,841 + EX31_RS01075 0.29 -1.3 234,842 - EX31_RS01075 0.29 -2.0 234,916 + EX31_RS01075 0.46 -0.4 234,970 - EX31_RS01075 0.58 -2.2 235,034 - EX31_RS01075 0.73 +0.7 235,034 - EX31_RS01075 0.73 -0.6 235,034 - EX31_RS01075 0.73 -1.2 235,034 - EX31_RS01075 0.73 +1.4 235,034 - EX31_RS01075 0.73 +0.3 235,034 - EX31_RS01075 0.73 -0.4 235,056 - EX31_RS01075 0.77 -1.7 235,056 - EX31_RS01075 0.77 -0.7 235,056 - EX31_RS01075 0.77 +2.2 235,056 - EX31_RS01075 0.77 -0.5 235,067 - EX31_RS01075 0.80 +0.1 235,067 - EX31_RS01075 0.80 -0.8 235,097 - EX31_RS01075 0.87 -0.4 235,130 - -0.2 235,157 - -1.2 235,187 + +0.6 235,200 + +1.2 235,201 - -0.5 235,224 + +0.3 235,224 + -0.2 235,224 + +0.1 235,225 - -1.3 235,233 + +0.7 235,233 + -0.4 235,233 + +0.5 235,233 + -0.7 235,234 - +0.8 235,234 - +0.2 235,234 - -0.6 235,234 - +0.6 235,234 - -0.1 235,244 - +0.2 235,280 + -0.7 235,290 + +0.2 235,292 + -0.8 235,292 + +1.5 235,303 + -0.7 235,371 + +0.2 235,379 + +0.2 235,543 - EX31_RS01080 0.21 -0.4 235,645 + EX31_RS01080 0.32 -1.5 235,732 + EX31_RS01080 0.41 -0.7 235,748 + EX31_RS01080 0.43 +0.3 235,748 + EX31_RS01080 0.43 +1.0 235,748 + EX31_RS01080 0.43 +0.8 235,748 + EX31_RS01080 0.43 -1.6 235,748 + EX31_RS01080 0.43 +0.1 235,749 - EX31_RS01080 0.43 +1.8 235,802 + EX31_RS01080 0.49 +0.2 235,802 + EX31_RS01080 0.49 +0.2 235,835 + EX31_RS01080 0.52 -1.0 235,860 - EX31_RS01080 0.55 +1.7 235,874 - EX31_RS01080 0.56 -0.7 235,982 + EX31_RS01080 0.68 -0.2 235,997 + EX31_RS01080 0.69 -1.1 236,015 + EX31_RS01080 0.71 -2.7 236,015 + EX31_RS01080 0.71 +0.1 236,015 + EX31_RS01080 0.71 +0.0 236,186 + EX31_RS01080 0.89 -1.4 236,186 + EX31_RS01080 0.89 -0.4 236,186 + EX31_RS01080 0.89 -0.8 236,186 + EX31_RS01080 0.89 -0.0 236,228 + +0.6 236,228 + +0.9 236,228 + -2.2 236,228 + +0.7 236,229 - +2.2 236,316 + +1.0 236,316 + +0.8 236,329 + -1.2 236,329 + +0.1 236,329 + -0.2 236,329 + +0.3 236,329 + +0.7 236,329 + +2.6 236,418 + EX31_RS01085 0.18 +1.2 236,433 + EX31_RS01085 0.19 +0.2 236,433 + EX31_RS01085 0.19 +1.1 236,527 + EX31_RS01085 0.32 +0.4 236,527 + EX31_RS01085 0.32 +2.2 236,528 - EX31_RS01085 0.32 -1.8 236,641 - EX31_RS01085 0.46 +1.0 236,965 - EX31_RS01085 0.88 -1.2 237,012 + +0.6 237,012 + -0.9 237,088 - +1.5 237,093 + -0.4 237,093 + -0.1 237,093 + -1.7 237,093 + +0.8 237,093 + -1.4 237,093 + +1.2 237,093 + +0.6 237,093 + +0.1 237,093 + -0.5 237,093 + -0.4 237,093 + +0.5 237,093 + +0.2 237,093 + -1.2 237,093 + -1.8 237,093 + +0.5 237,093 + +0.2 237,093 + +0.4 237,093 + +1.7 237,093 + +0.4 237,093 + -1.4 237,094 - -0.8 237,095 + -0.4 237,095 + +1.0 237,096 - +1.2 237,276 - EX31_RS01090 0.26 +1.2
Or see this region's nucleotide sequence