Experiment: reconstituted MRS with Amylopectin from maize 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14220 and BBR_RS14225 are separated by 71 nucleotides BBR_RS14225 and BBR_RS14230 are separated by 57 nucleotides
BBR_RS14220: BBR_RS14220 - phosphomannomutase, at 965,609 to 966,412
_RS14220
BBR_RS14225: BBR_RS14225 - ComF family protein, at 966,484 to 967,158
_RS14225
BBR_RS14230: BBR_RS14230 - HAMP domain-containing histidine kinase, at 967,216 to 968,289
_RS14230
Position (kb)
966
967
968 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 965.503 kb on + strand at 965.570 kb on - strand at 965.589 kb on - strand at 965.620 kb on + strand at 965.673 kb on + strand at 965.678 kb on + strand at 965.681 kb on - strand at 965.700 kb on + strand, within BBR_RS14220 at 965.700 kb on + strand, within BBR_RS14220 at 965.703 kb on - strand, within BBR_RS14220 at 965.708 kb on - strand, within BBR_RS14220 at 965.710 kb on + strand, within BBR_RS14220 at 965.786 kb on + strand, within BBR_RS14220 at 965.810 kb on - strand, within BBR_RS14220 at 965.834 kb on - strand, within BBR_RS14220 at 965.862 kb on - strand, within BBR_RS14220 at 965.865 kb on + strand, within BBR_RS14220 at 965.883 kb on + strand, within BBR_RS14220 at 965.891 kb on + strand, within BBR_RS14220 at 965.897 kb on - strand, within BBR_RS14220 at 965.919 kb on - strand, within BBR_RS14220 at 965.961 kb on + strand, within BBR_RS14220 at 965.984 kb on - strand, within BBR_RS14220 at 966.096 kb on - strand, within BBR_RS14220 at 966.113 kb on - strand, within BBR_RS14220 at 966.215 kb on + strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.234 kb on - strand, within BBR_RS14220 at 966.281 kb on + strand, within BBR_RS14220 at 966.299 kb on - strand, within BBR_RS14220 at 966.367 kb on + strand at 966.367 kb on + strand at 966.386 kb on - strand at 966.391 kb on + strand at 966.409 kb on - strand at 966.509 kb on - strand at 966.528 kb on - strand at 966.530 kb on + strand at 966.546 kb on - strand at 966.546 kb on - strand at 966.551 kb on - strand at 966.551 kb on - strand at 966.627 kb on + strand, within BBR_RS14225 at 966.635 kb on - strand, within BBR_RS14225 at 966.635 kb on - strand, within BBR_RS14225 at 966.732 kb on - strand, within BBR_RS14225 at 966.830 kb on + strand, within BBR_RS14225 at 966.833 kb on - strand, within BBR_RS14225 at 966.840 kb on - strand, within BBR_RS14225 at 966.863 kb on - strand, within BBR_RS14225 at 966.863 kb on - strand, within BBR_RS14225 at 966.926 kb on - strand, within BBR_RS14225 at 967.025 kb on + strand, within BBR_RS14225 at 967.039 kb on - strand, within BBR_RS14225 at 967.053 kb on + strand, within BBR_RS14225 at 967.064 kb on + strand, within BBR_RS14225 at 967.074 kb on + strand, within BBR_RS14225 at 967.106 kb on + strand at 967.208 kb on + strand at 967.278 kb on + strand at 967.284 kb on + strand at 967.332 kb on + strand, within BBR_RS14230 at 967.340 kb on - strand, within BBR_RS14230 at 967.360 kb on + strand, within BBR_RS14230 at 967.365 kb on + strand, within BBR_RS14230 at 967.479 kb on - strand, within BBR_RS14230 at 967.503 kb on + strand, within BBR_RS14230 at 967.509 kb on - strand, within BBR_RS14230 at 967.541 kb on + strand, within BBR_RS14230 at 967.608 kb on - strand, within BBR_RS14230 at 967.609 kb on + strand, within BBR_RS14230 at 967.681 kb on - strand, within BBR_RS14230 at 967.698 kb on - strand, within BBR_RS14230 at 967.707 kb on - strand, within BBR_RS14230 at 967.715 kb on + strand, within BBR_RS14230 at 967.763 kb on - strand, within BBR_RS14230 at 967.763 kb on - strand, within BBR_RS14230 at 967.830 kb on - strand, within BBR_RS14230 at 967.863 kb on + strand, within BBR_RS14230 at 967.915 kb on - strand, within BBR_RS14230 at 967.919 kb on - strand, within BBR_RS14230 at 967.919 kb on - strand, within BBR_RS14230 at 967.920 kb on - strand, within BBR_RS14230 at 967.920 kb on - strand, within BBR_RS14230 at 968.005 kb on - strand, within BBR_RS14230 at 968.014 kb on - strand, within BBR_RS14230 at 968.043 kb on - strand, within BBR_RS14230 at 968.102 kb on - strand, within BBR_RS14230 at 968.117 kb on + strand, within BBR_RS14230
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Amylopectin from maize 10 mg/ml remove 965,503 + -0.7 965,570 - -0.5 965,589 - +0.0 965,620 + +1.2 965,673 + -0.7 965,678 + -1.3 965,681 - -1.6 965,700 + BBR_RS14220 0.11 -1.6 965,700 + BBR_RS14220 0.11 -2.0 965,703 - BBR_RS14220 0.12 -1.6 965,708 - BBR_RS14220 0.12 -1.7 965,710 + BBR_RS14220 0.13 -2.8 965,786 + BBR_RS14220 0.22 -1.5 965,810 - BBR_RS14220 0.25 -1.8 965,834 - BBR_RS14220 0.28 -2.0 965,862 - BBR_RS14220 0.31 -0.7 965,865 + BBR_RS14220 0.32 -2.0 965,883 + BBR_RS14220 0.34 -2.3 965,891 + BBR_RS14220 0.35 -1.5 965,897 - BBR_RS14220 0.36 -1.6 965,919 - BBR_RS14220 0.39 -0.9 965,961 + BBR_RS14220 0.44 -3.2 965,984 - BBR_RS14220 0.47 -0.8 966,096 - BBR_RS14220 0.61 -1.5 966,113 - BBR_RS14220 0.63 -2.1 966,215 + BBR_RS14220 0.75 -2.3 966,222 - BBR_RS14220 0.76 -1.8 966,222 - BBR_RS14220 0.76 -0.7 966,222 - BBR_RS14220 0.76 -2.0 966,234 - BBR_RS14220 0.78 -1.2 966,281 + BBR_RS14220 0.84 -2.9 966,299 - BBR_RS14220 0.86 -1.6 966,367 + -1.9 966,367 + +0.4 966,386 - -0.7 966,391 + +1.1 966,409 - +0.9 966,509 - +0.2 966,528 - +0.1 966,530 + +0.2 966,546 - -0.5 966,546 - -0.1 966,551 - +1.2 966,551 - -0.4 966,627 + BBR_RS14225 0.21 -0.1 966,635 - BBR_RS14225 0.22 -1.8 966,635 - BBR_RS14225 0.22 -0.7 966,732 - BBR_RS14225 0.37 -1.6 966,830 + BBR_RS14225 0.51 -0.8 966,833 - BBR_RS14225 0.52 -0.1 966,840 - BBR_RS14225 0.53 -0.3 966,863 - BBR_RS14225 0.56 -0.4 966,863 - BBR_RS14225 0.56 -1.7 966,926 - BBR_RS14225 0.65 +0.6 967,025 + BBR_RS14225 0.80 -1.0 967,039 - BBR_RS14225 0.82 -0.1 967,053 + BBR_RS14225 0.84 -0.2 967,064 + BBR_RS14225 0.86 -0.3 967,074 + BBR_RS14225 0.87 +0.7 967,106 + +0.4 967,208 + +0.6 967,278 + -1.8 967,284 + -1.3 967,332 + BBR_RS14230 0.11 -2.1 967,340 - BBR_RS14230 0.12 -2.1 967,360 + BBR_RS14230 0.13 -1.3 967,365 + BBR_RS14230 0.14 -0.5 967,479 - BBR_RS14230 0.24 -1.1 967,503 + BBR_RS14230 0.27 -2.4 967,509 - BBR_RS14230 0.27 -3.1 967,541 + BBR_RS14230 0.30 -2.3 967,608 - BBR_RS14230 0.36 -1.6 967,609 + BBR_RS14230 0.37 -1.3 967,681 - BBR_RS14230 0.43 -1.0 967,698 - BBR_RS14230 0.45 -1.9 967,707 - BBR_RS14230 0.46 -0.1 967,715 + BBR_RS14230 0.46 -2.0 967,763 - BBR_RS14230 0.51 -1.7 967,763 - BBR_RS14230 0.51 -1.1 967,830 - BBR_RS14230 0.57 -1.2 967,863 + BBR_RS14230 0.60 -1.3 967,915 - BBR_RS14230 0.65 -1.7 967,919 - BBR_RS14230 0.65 -0.6 967,919 - BBR_RS14230 0.65 -1.8 967,920 - BBR_RS14230 0.66 -1.0 967,920 - BBR_RS14230 0.66 -1.5 968,005 - BBR_RS14230 0.73 -2.0 968,014 - BBR_RS14230 0.74 -1.0 968,043 - BBR_RS14230 0.77 +0.4 968,102 - BBR_RS14230 0.82 -0.8 968,117 + BBR_RS14230 0.84 -1.3
Or see this region's nucleotide sequence