Experiment: reconstituted MRS with Amylopectin from maize 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10895 and BBR_RS10900 are separated by 246 nucleotides BBR_RS10900 and BBR_RS10905 overlap by 1 nucleotides BBR_RS10905 and BBR_RS10910 are separated by 178 nucleotides
BBR_RS10895: BBR_RS10895 - type I pullulanase, at 183,482 to 188,608
_RS10895
BBR_RS10900: BBR_RS10900 - molecular chaperone DnaK, at 188,855 to 190,735
_RS10900
BBR_RS10905: BBR_RS10905 - nucleotide exchange factor GrpE, at 190,735 to 191,418
_RS10905
BBR_RS10910: BBR_RS10910 - molecular chaperone DnaJ, at 191,597 to 192,610
_RS10910
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2 at 187.864 kb on + strand, within BBR_RS10895 at 187.867 kb on - strand, within BBR_RS10895 at 187.867 kb on - strand, within BBR_RS10895 at 187.872 kb on - strand, within BBR_RS10895 at 187.886 kb on + strand, within BBR_RS10895 at 187.898 kb on + strand, within BBR_RS10895 at 187.903 kb on + strand, within BBR_RS10895 at 187.906 kb on - strand, within BBR_RS10895 at 187.913 kb on + strand, within BBR_RS10895 at 187.914 kb on - strand, within BBR_RS10895 at 187.921 kb on + strand, within BBR_RS10895 at 187.950 kb on + strand, within BBR_RS10895 at 187.955 kb on + strand, within BBR_RS10895 at 187.955 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.968 kb on - strand, within BBR_RS10895 at 187.977 kb on + strand, within BBR_RS10895 at 187.977 kb on + strand, within BBR_RS10895 at 187.982 kb on + strand, within BBR_RS10895 at 187.984 kb on - strand, within BBR_RS10895 at 187.987 kb on + strand, within BBR_RS10895 at 187.992 kb on + strand, within BBR_RS10895 at 187.992 kb on + strand, within BBR_RS10895 at 187.992 kb on + strand, within BBR_RS10895 at 187.994 kb on + strand, within BBR_RS10895 at 187.994 kb on + strand, within BBR_RS10895 at 187.995 kb on + strand, within BBR_RS10895 at 187.995 kb on - strand, within BBR_RS10895 at 188.000 kb on - strand, within BBR_RS10895 at 188.000 kb on - strand, within BBR_RS10895 at 188.000 kb on - strand, within BBR_RS10895 at 188.011 kb on + strand, within BBR_RS10895 at 188.013 kb on + strand, within BBR_RS10895 at 188.014 kb on + strand, within BBR_RS10895 at 188.019 kb on - strand, within BBR_RS10895 at 188.019 kb on - strand, within BBR_RS10895 at 188.021 kb on + strand, within BBR_RS10895 at 188.021 kb on + strand, within BBR_RS10895 at 188.024 kb on - strand, within BBR_RS10895 at 188.026 kb on + strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.032 kb on + strand, within BBR_RS10895 at 188.032 kb on + strand, within BBR_RS10895 at 188.032 kb on + strand, within BBR_RS10895 at 188.040 kb on - strand, within BBR_RS10895 at 188.040 kb on - strand, within BBR_RS10895 at 188.048 kb on - strand, within BBR_RS10895 at 188.050 kb on - strand, within BBR_RS10895 at 188.050 kb on - strand, within BBR_RS10895 at 188.076 kb on + strand, within BBR_RS10895 at 188.079 kb on + strand, within BBR_RS10895 at 188.087 kb on - strand, within BBR_RS10895 at 188.094 kb on + strand, within BBR_RS10895 at 188.097 kb on - strand at 188.097 kb on - strand at 188.124 kb on - strand at 188.135 kb on + strand at 188.135 kb on + strand at 188.137 kb on + strand at 188.137 kb on + strand at 188.145 kb on - strand at 188.145 kb on - strand at 188.145 kb on - strand at 188.147 kb on + strand at 188.147 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.190 kb on - strand at 188.190 kb on - strand at 188.190 kb on - strand at 188.190 kb on - strand at 188.191 kb on - strand at 188.192 kb on + strand at 188.213 kb on + strand at 188.232 kb on - strand at 188.238 kb on + strand at 188.238 kb on + strand at 188.244 kb on - strand at 188.246 kb on - strand at 188.246 kb on - strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.256 kb on - strand at 188.256 kb on - strand at 188.278 kb on - strand at 188.304 kb on + strand at 188.304 kb on + strand at 188.305 kb on - strand at 188.359 kb on + strand at 188.364 kb on + strand at 188.368 kb on - strand at 188.368 kb on - strand at 188.369 kb on + strand at 188.369 kb on + strand at 188.369 kb on + strand at 188.370 kb on - strand at 188.377 kb on - strand at 188.377 kb on - strand at 188.391 kb on + strand at 188.391 kb on + strand at 188.391 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.399 kb on - strand at 188.401 kb on + strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.413 kb on - strand at 188.413 kb on - strand at 188.417 kb on + strand at 188.425 kb on - strand at 188.451 kb on + strand at 188.451 kb on + strand at 188.459 kb on - strand at 188.461 kb on + strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.477 kb on + strand at 188.477 kb on + strand at 188.477 kb on + strand at 188.479 kb on + strand at 188.479 kb on + strand at 188.482 kb on - strand at 188.485 kb on + strand at 188.485 kb on - strand at 188.485 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.494 kb on + strand at 188.495 kb on + strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.519 kb on + strand at 188.532 kb on - strand at 188.567 kb on - strand at 188.574 kb on - strand at 188.577 kb on - strand at 188.586 kb on + strand at 188.591 kb on - strand at 188.592 kb on - strand at 188.595 kb on + strand at 188.595 kb on + strand at 188.596 kb on - strand at 188.598 kb on + strand at 188.603 kb on + strand at 188.603 kb on + strand at 188.605 kb on + strand at 188.605 kb on + strand at 188.605 kb on + strand at 188.605 kb on + strand at 188.607 kb on + strand at 188.608 kb on + strand at 188.608 kb on - strand at 188.608 kb on - strand at 188.608 kb on - strand at 188.611 kb on - strand at 188.611 kb on - strand at 188.611 kb on - strand at 188.611 kb on - strand at 188.613 kb on - strand at 188.613 kb on - strand at 188.613 kb on - strand at 188.615 kb on - strand at 188.623 kb on - strand at 188.670 kb on + strand at 188.672 kb on - strand at 188.675 kb on + strand at 188.683 kb on - strand at 188.737 kb on - strand at 188.825 kb on + strand at 188.825 kb on + strand at 190.516 kb on + strand, within BBR_RS10900 at 190.516 kb on + strand, within BBR_RS10900 at 190.516 kb on + strand, within BBR_RS10900 at 190.522 kb on + strand, within BBR_RS10900 at 190.530 kb on + strand, within BBR_RS10900 at 190.540 kb on + strand, within BBR_RS10900 at 190.540 kb on + strand, within BBR_RS10900 at 190.549 kb on + strand at 190.555 kb on + strand at 190.555 kb on + strand at 190.569 kb on + strand at 190.599 kb on + strand at 190.599 kb on + strand at 190.611 kb on + strand at 190.611 kb on + strand at 190.815 kb on + strand, within BBR_RS10905 at 190.815 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.998 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.108 kb on + strand, within BBR_RS10905 at 191.305 kb on + strand, within BBR_RS10905 at 191.390 kb on + strand at 191.395 kb on + strand at 191.422 kb on + strand at 191.425 kb on - strand at 191.425 kb on - strand at 191.433 kb on + strand at 191.472 kb on - strand at 191.478 kb on + strand at 191.478 kb on + strand at 191.484 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.499 kb on + strand at 191.499 kb on + strand at 191.507 kb on - strand at 191.519 kb on - strand at 191.527 kb on + strand at 191.535 kb on - strand at 191.543 kb on - strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.553 kb on - strand at 191.553 kb on - strand at 191.567 kb on + strand at 191.587 kb on + strand at 191.603 kb on + strand at 191.608 kb on - strand at 191.619 kb on + strand at 191.626 kb on + strand at 191.626 kb on + strand at 191.636 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.655 kb on - strand at 191.655 kb on - strand at 191.664 kb on - strand at 191.701 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.710 kb on + strand, within BBR_RS10910 at 191.712 kb on - strand, within BBR_RS10910 at 191.719 kb on + strand, within BBR_RS10910
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Amylopectin from maize 10 mg/ml remove 187,864 + BBR_RS10895 0.85 +0.8 187,867 - BBR_RS10895 0.86 +0.3 187,867 - BBR_RS10895 0.86 +0.7 187,872 - BBR_RS10895 0.86 -1.5 187,886 + BBR_RS10895 0.86 +0.0 187,898 + BBR_RS10895 0.86 +0.3 187,903 + BBR_RS10895 0.86 +0.3 187,906 - BBR_RS10895 0.86 +0.6 187,913 + BBR_RS10895 0.86 +1.2 187,914 - BBR_RS10895 0.86 -0.7 187,921 + BBR_RS10895 0.87 -0.2 187,950 + BBR_RS10895 0.87 -0.1 187,955 + BBR_RS10895 0.87 +1.1 187,955 + BBR_RS10895 0.87 -0.5 187,960 + BBR_RS10895 0.87 -0.7 187,960 + BBR_RS10895 0.87 -1.1 187,960 + BBR_RS10895 0.87 -0.2 187,960 + BBR_RS10895 0.87 -0.1 187,960 + BBR_RS10895 0.87 -0.4 187,968 - BBR_RS10895 0.87 -0.5 187,977 + BBR_RS10895 0.88 -1.0 187,977 + BBR_RS10895 0.88 -0.3 187,982 + BBR_RS10895 0.88 -0.9 187,984 - BBR_RS10895 0.88 +0.6 187,987 + BBR_RS10895 0.88 -0.3 187,992 + BBR_RS10895 0.88 -0.2 187,992 + BBR_RS10895 0.88 +0.4 187,992 + BBR_RS10895 0.88 -0.0 187,994 + BBR_RS10895 0.88 +0.2 187,994 + BBR_RS10895 0.88 -0.3 187,995 + BBR_RS10895 0.88 +0.1 187,995 - BBR_RS10895 0.88 -0.6 188,000 - BBR_RS10895 0.88 +0.1 188,000 - BBR_RS10895 0.88 -0.1 188,000 - BBR_RS10895 0.88 +0.5 188,011 + BBR_RS10895 0.88 -1.6 188,013 + BBR_RS10895 0.88 -0.3 188,014 + BBR_RS10895 0.88 +0.6 188,019 - BBR_RS10895 0.88 +1.1 188,019 - BBR_RS10895 0.88 +0.8 188,021 + BBR_RS10895 0.89 +0.2 188,021 + BBR_RS10895 0.89 +0.9 188,024 - BBR_RS10895 0.89 +0.7 188,026 + BBR_RS10895 0.89 +0.4 188,029 - BBR_RS10895 0.89 +0.9 188,029 - BBR_RS10895 0.89 -1.8 188,029 - BBR_RS10895 0.89 -1.5 188,029 - BBR_RS10895 0.89 +0.7 188,032 + BBR_RS10895 0.89 +0.4 188,032 + BBR_RS10895 0.89 -0.6 188,032 + BBR_RS10895 0.89 +0.4 188,040 - BBR_RS10895 0.89 +0.4 188,040 - BBR_RS10895 0.89 -0.8 188,048 - BBR_RS10895 0.89 +0.0 188,050 - BBR_RS10895 0.89 -0.6 188,050 - BBR_RS10895 0.89 -1.4 188,076 + BBR_RS10895 0.90 +0.6 188,079 + BBR_RS10895 0.90 +1.3 188,087 - BBR_RS10895 0.90 -0.6 188,094 + BBR_RS10895 0.90 -0.7 188,097 - +0.2 188,097 - -0.8 188,124 - +0.1 188,135 + -1.1 188,135 + -1.0 188,137 + -1.0 188,137 + -1.7 188,145 - -1.7 188,145 - +0.6 188,145 - -0.3 188,147 + +0.5 188,147 + +0.6 188,182 + +0.8 188,182 + -0.1 188,182 + +0.5 188,182 + -0.4 188,182 + +0.7 188,182 + -1.3 188,190 - -0.3 188,190 - -0.8 188,190 - +1.7 188,190 - -0.7 188,191 - +0.9 188,192 + -0.1 188,213 + -0.3 188,232 - +0.7 188,238 + +0.4 188,238 + +1.1 188,244 - -0.7 188,246 - -0.4 188,246 - +0.0 188,248 + -1.8 188,248 + +0.5 188,248 + -0.8 188,248 + +0.1 188,248 + -1.4 188,248 + -1.2 188,256 - -0.9 188,256 - +1.1 188,278 - +0.1 188,304 + -0.3 188,304 + -0.7 188,305 - +0.5 188,359 + -0.6 188,364 + -0.0 188,368 - -1.1 188,368 - -0.8 188,369 + -1.2 188,369 + +0.4 188,369 + -1.0 188,370 - -0.2 188,377 - -0.3 188,377 - +0.5 188,391 + -0.5 188,391 + -0.0 188,391 + -0.1 188,396 + -0.8 188,396 + +0.7 188,396 + +0.2 188,396 + +1.3 188,396 + +1.6 188,399 - -1.6 188,401 + +1.1 188,404 - -0.5 188,404 - -0.7 188,404 - -0.1 188,404 - -0.6 188,404 - +0.6 188,404 - -1.7 188,413 - -0.0 188,413 - -0.3 188,417 + -0.4 188,425 - +0.4 188,451 + -0.9 188,451 + +1.6 188,459 - -0.2 188,461 + -1.4 188,469 - +0.1 188,469 - +0.4 188,469 - -1.3 188,469 - -0.3 188,469 - +0.1 188,477 + -0.4 188,477 + -0.7 188,477 + -0.5 188,479 + +0.1 188,479 + -1.0 188,482 - +1.4 188,485 + -1.0 188,485 - -0.0 188,485 - -0.1 188,487 - +0.1 188,487 - +0.3 188,487 - +0.2 188,487 - -0.3 188,487 - +1.3 188,487 - +1.0 188,490 + -2.6 188,490 + -1.4 188,490 + +0.2 188,490 + -0.4 188,490 + -0.2 188,490 + +0.8 188,490 + -0.2 188,490 + +0.1 188,494 + +0.3 188,495 + +0.2 188,498 - +0.2 188,498 - +0.4 188,498 - +1.0 188,498 - -0.7 188,498 - -1.4 188,519 + -0.4 188,532 - +0.2 188,567 - -0.9 188,574 - +0.4 188,577 - -0.7 188,586 + +0.9 188,591 - -0.7 188,592 - -0.2 188,595 + -0.8 188,595 + +0.5 188,596 - -1.0 188,598 + -0.1 188,603 + -1.1 188,603 + -2.1 188,605 + +0.4 188,605 + +0.3 188,605 + +0.8 188,605 + +0.1 188,607 + -1.3 188,608 + +1.3 188,608 - -0.8 188,608 - +0.4 188,608 - +0.6 188,611 - -0.2 188,611 - -0.5 188,611 - +1.6 188,611 - -0.2 188,613 - +0.6 188,613 - -1.0 188,613 - +0.1 188,615 - +0.4 188,623 - -0.3 188,670 + +1.5 188,672 - -1.1 188,675 + +0.1 188,683 - -1.1 188,737 - +0.2 188,825 + +0.8 188,825 + +0.3 190,516 + BBR_RS10900 0.88 +0.4 190,516 + BBR_RS10900 0.88 -0.7 190,516 + BBR_RS10900 0.88 -0.3 190,522 + BBR_RS10900 0.89 -0.6 190,530 + BBR_RS10900 0.89 -2.0 190,540 + BBR_RS10900 0.90 -2.2 190,540 + BBR_RS10900 0.90 +0.4 190,549 + -0.3 190,555 + -0.1 190,555 + -0.1 190,569 + -1.9 190,599 + +1.6 190,599 + -1.1 190,611 + +1.4 190,611 + -1.0 190,815 + BBR_RS10905 0.12 +0.6 190,815 + BBR_RS10905 0.12 +0.6 190,845 + BBR_RS10905 0.16 -0.7 190,845 + BBR_RS10905 0.16 -0.8 190,845 + BBR_RS10905 0.16 -1.3 190,860 + BBR_RS10905 0.18 -0.1 190,860 + BBR_RS10905 0.18 -1.3 190,998 + BBR_RS10905 0.38 +0.2 191,022 + BBR_RS10905 0.42 -0.3 191,022 + BBR_RS10905 0.42 +0.5 191,022 + BBR_RS10905 0.42 -1.1 191,022 + BBR_RS10905 0.42 -1.2 191,108 + BBR_RS10905 0.55 +0.3 191,305 + BBR_RS10905 0.83 +0.7 191,390 + +0.5 191,395 + +1.2 191,422 + -0.7 191,425 - -0.5 191,425 - +0.6 191,433 + +0.8 191,472 - -1.0 191,478 + +0.5 191,478 + +0.5 191,484 + -0.7 191,488 + -0.0 191,488 + -0.5 191,488 + -1.6 191,488 + +0.8 191,488 + -0.1 191,496 - +1.9 191,496 - +0.1 191,496 - -0.9 191,499 + -0.8 191,499 + -0.0 191,507 - -0.6 191,519 - -0.2 191,527 + -0.7 191,535 - +2.2 191,543 - +0.4 191,545 + +0.1 191,545 + +0.1 191,545 + +0.5 191,545 + -0.7 191,545 + -1.2 191,553 - +0.5 191,553 - +1.4 191,567 + -0.3 191,587 + +1.1 191,603 + +1.4 191,608 - -0.4 191,619 + -1.2 191,626 + +0.1 191,626 + +0.7 191,636 + +0.7 191,647 + +0.4 191,647 + +0.0 191,647 + +0.4 191,655 - +0.2 191,655 - -0.5 191,664 - -0.2 191,701 + BBR_RS10910 0.10 +1.7 191,704 + BBR_RS10910 0.11 +1.7 191,704 + BBR_RS10910 0.11 -1.1 191,704 + BBR_RS10910 0.11 +1.4 191,704 + BBR_RS10910 0.11 +0.5 191,704 + BBR_RS10910 0.11 +2.0 191,704 + BBR_RS10910 0.11 +0.0 191,709 + BBR_RS10910 0.11 +0.3 191,709 + BBR_RS10910 0.11 +0.7 191,709 + BBR_RS10910 0.11 +1.7 191,710 + BBR_RS10910 0.11 +0.0 191,712 - BBR_RS10910 0.11 +0.1 191,719 + BBR_RS10910 0.12 +0.2
Or see this region's nucleotide sequence