Experiment: Cobalt chloride 0.32 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25665 and AO353_25670 are separated by 3 nucleotides AO353_25670 and AO353_25675 are separated by 96 nucleotides AO353_25675 and AO353_25680 are separated by 13 nucleotides
AO353_25665: AO353_25665 - 3-hydroxy-isobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 5,659,716 to 5,660,822
_25665
AO353_25670: AO353_25670 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,660,826 to 5,661,977
_25670
AO353_25675: AO353_25675 - enoyl-CoA hydratase, at 5,662,074 to 5,662,847
_25675
AO353_25680: AO353_25680 - 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (from data) , at 5,662,861 to 5,663,988
_25680
Position (kb)
5660
5661
5662 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5660.025 kb on + strand, within AO353_25665 at 5660.026 kb on - strand, within AO353_25665 at 5660.181 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.286 kb on + strand, within AO353_25665 at 5660.286 kb on + strand, within AO353_25665 at 5660.287 kb on - strand, within AO353_25665 at 5660.308 kb on - strand, within AO353_25665 at 5660.396 kb on - strand, within AO353_25665 at 5660.414 kb on - strand, within AO353_25665 at 5660.561 kb on - strand, within AO353_25665 at 5660.594 kb on - strand, within AO353_25665 at 5661.015 kb on + strand, within AO353_25670 at 5661.015 kb on + strand, within AO353_25670 at 5661.015 kb on + strand, within AO353_25670 at 5661.015 kb on + strand, within AO353_25670 at 5661.016 kb on - strand, within AO353_25670 at 5661.016 kb on - strand, within AO353_25670 at 5661.016 kb on - strand, within AO353_25670 at 5661.024 kb on + strand, within AO353_25670 at 5661.024 kb on + strand, within AO353_25670 at 5661.024 kb on + strand, within AO353_25670 at 5661.025 kb on - strand, within AO353_25670 at 5661.033 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.130 kb on - strand, within AO353_25670 at 5661.130 kb on - strand, within AO353_25670 at 5661.407 kb on + strand, within AO353_25670 at 5661.407 kb on + strand, within AO353_25670 at 5661.883 kb on - strand at 5661.883 kb on - strand at 5661.885 kb on + strand at 5661.912 kb on + strand at 5661.912 kb on + strand at 5661.912 kb on + strand at 5661.912 kb on + strand at 5661.913 kb on - strand at 5661.913 kb on - strand at 5661.967 kb on - strand at 5662.011 kb on + strand at 5662.030 kb on + strand at 5662.031 kb on - strand at 5662.031 kb on - strand at 5662.075 kb on + strand at 5662.075 kb on + strand at 5662.075 kb on + strand at 5662.075 kb on + strand at 5662.076 kb on - strand at 5662.076 kb on - strand at 5662.076 kb on - strand at 5662.076 kb on - strand at 5662.076 kb on - strand at 5662.612 kb on + strand, within AO353_25675 at 5662.841 kb on - strand at 5662.910 kb on + strand at 5662.911 kb on - strand at 5662.911 kb on - strand at 5662.911 kb on - strand at 5662.911 kb on - strand at 5662.911 kb on - strand at 5662.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.32 mM remove 5,660,025 + AO353_25665 0.28 +1.9 5,660,026 - AO353_25665 0.28 -1.5 5,660,181 + AO353_25665 0.42 +0.4 5,660,205 + AO353_25665 0.44 +0.8 5,660,205 + AO353_25665 0.44 +0.8 5,660,205 + AO353_25665 0.44 +0.7 5,660,205 + AO353_25665 0.44 +0.7 5,660,241 + AO353_25665 0.47 +1.0 5,660,241 + AO353_25665 0.47 -0.5 5,660,241 + AO353_25665 0.47 +0.5 5,660,241 + AO353_25665 0.47 -1.0 5,660,241 + AO353_25665 0.47 +0.4 5,660,286 + AO353_25665 0.51 -1.4 5,660,286 + AO353_25665 0.51 +0.4 5,660,287 - AO353_25665 0.52 -0.3 5,660,308 - AO353_25665 0.53 -1.1 5,660,396 - AO353_25665 0.61 -0.1 5,660,414 - AO353_25665 0.63 -1.8 5,660,561 - AO353_25665 0.76 -0.5 5,660,594 - AO353_25665 0.79 +1.7 5,661,015 + AO353_25670 0.16 +0.0 5,661,015 + AO353_25670 0.16 -2.4 5,661,015 + AO353_25670 0.16 -2.1 5,661,015 + AO353_25670 0.16 -0.1 5,661,016 - AO353_25670 0.16 -0.6 5,661,016 - AO353_25670 0.16 +0.1 5,661,016 - AO353_25670 0.16 -1.1 5,661,024 + AO353_25670 0.17 -1.4 5,661,024 + AO353_25670 0.17 -1.1 5,661,024 + AO353_25670 0.17 -1.1 5,661,025 - AO353_25670 0.17 +0.6 5,661,033 + AO353_25670 0.18 +0.9 5,661,129 + AO353_25670 0.26 +1.1 5,661,129 + AO353_25670 0.26 +0.0 5,661,129 + AO353_25670 0.26 -1.1 5,661,129 + AO353_25670 0.26 -0.5 5,661,129 + AO353_25670 0.26 -1.8 5,661,129 + AO353_25670 0.26 -1.1 5,661,130 - AO353_25670 0.26 -0.6 5,661,130 - AO353_25670 0.26 -1.0 5,661,407 + AO353_25670 0.50 +0.9 5,661,407 + AO353_25670 0.50 -2.4 5,661,883 - -0.8 5,661,883 - +0.9 5,661,885 + +1.2 5,661,912 + -0.6 5,661,912 + -1.6 5,661,912 + +0.0 5,661,912 + +1.4 5,661,913 - +0.5 5,661,913 - +1.5 5,661,967 - +0.5 5,662,011 + -0.4 5,662,030 + -1.1 5,662,031 - -1.1 5,662,031 - +0.6 5,662,075 + -0.9 5,662,075 + -0.4 5,662,075 + -0.9 5,662,075 + +1.0 5,662,076 - +0.7 5,662,076 - -1.4 5,662,076 - +1.4 5,662,076 - -0.3 5,662,076 - +0.4 5,662,612 + AO353_25675 0.70 +0.8 5,662,841 - +0.1 5,662,910 + -0.6 5,662,911 - -0.4 5,662,911 - -0.1 5,662,911 - +0.1 5,662,911 - -0.1 5,662,911 - -1.2 5,662,938 - -1.1
Or see this region's nucleotide sequence