Experiment: Beta-Lactose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01395 and HMPREF1058_RS01390 overlap by 8 nucleotides HMPREF1058_RS01390 and HMPREF1058_RS01385 overlap by 19 nucleotides HMPREF1058_RS01385 and HMPREF1058_RS01380 are separated by 35 nucleotides HMPREF1058_RS01380 and HMPREF1058_RS01375 are separated by 116 nucleotides
HMPREF1058_RS01395: HMPREF1058_RS01395 - cobyric acid synthase, at 138,766 to 140,256
_RS01395
HMPREF1058_RS01390: HMPREF1058_RS01390 - pyridoxal phosphate-dependent class II aminotransferase, at 140,249 to 141,259
_RS01390
HMPREF1058_RS01385: HMPREF1058_RS01385 - cobalamin biosynthesis protein CobD, at 141,241 to 142,173
_RS01385
HMPREF1058_RS01380: HMPREF1058_RS01380 - tRNA-Gly, at 142,209 to 142,281
_RS01380
HMPREF1058_RS01375: HMPREF1058_RS01375 - site-specific integrase, at 142,398 to 143,615
_RS01375
Position (kb) 141
142
143 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 140.276 kb on + strand at 140.282 kb on + strand at 140.320 kb on + strand at 140.381 kb on - strand, within HMPREF1058_RS01390 at 140.402 kb on + strand, within HMPREF1058_RS01390 at 140.578 kb on + strand, within HMPREF1058_RS01390 at 140.590 kb on + strand, within HMPREF1058_RS01390 at 140.591 kb on - strand, within HMPREF1058_RS01390 at 140.591 kb on - strand, within HMPREF1058_RS01390 at 140.597 kb on - strand at 140.597 kb on - strand, within HMPREF1058_RS01390 at 140.697 kb on + strand, within HMPREF1058_RS01390 at 140.697 kb on + strand, within HMPREF1058_RS01390 at 140.856 kb on - strand, within HMPREF1058_RS01390 at 140.858 kb on - strand, within HMPREF1058_RS01390 at 140.946 kb on - strand, within HMPREF1058_RS01390 at 140.988 kb on + strand, within HMPREF1058_RS01390 at 141.068 kb on - strand, within HMPREF1058_RS01390 at 141.068 kb on - strand, within HMPREF1058_RS01390 at 141.193 kb on + strand at 141.249 kb on - strand at 141.418 kb on - strand, within HMPREF1058_RS01385 at 141.418 kb on - strand, within HMPREF1058_RS01385 at 141.537 kb on + strand, within HMPREF1058_RS01385 at 141.544 kb on - strand, within HMPREF1058_RS01385 at 141.555 kb on - strand, within HMPREF1058_RS01385 at 141.621 kb on - strand, within HMPREF1058_RS01385 at 141.717 kb on - strand, within HMPREF1058_RS01385 at 141.759 kb on + strand, within HMPREF1058_RS01385 at 141.759 kb on + strand, within HMPREF1058_RS01385 at 141.772 kb on - strand, within HMPREF1058_RS01385 at 141.789 kb on - strand, within HMPREF1058_RS01385 at 141.795 kb on + strand, within HMPREF1058_RS01385 at 141.934 kb on + strand, within HMPREF1058_RS01385 at 141.935 kb on - strand, within HMPREF1058_RS01385 at 141.935 kb on - strand, within HMPREF1058_RS01385 at 142.042 kb on + strand, within HMPREF1058_RS01385 at 142.053 kb on + strand, within HMPREF1058_RS01385 at 142.053 kb on + strand, within HMPREF1058_RS01385 at 142.063 kb on - strand, within HMPREF1058_RS01385 at 142.095 kb on + strand at 142.110 kb on - strand at 142.110 kb on - strand at 142.139 kb on + strand at 142.178 kb on + strand at 142.302 kb on - strand at 142.412 kb on + strand at 142.412 kb on + strand at 142.582 kb on - strand, within HMPREF1058_RS01375 at 142.595 kb on + strand, within HMPREF1058_RS01375 at 142.659 kb on + strand, within HMPREF1058_RS01375 at 142.746 kb on + strand, within HMPREF1058_RS01375 at 142.748 kb on + strand, within HMPREF1058_RS01375 at 142.778 kb on + strand, within HMPREF1058_RS01375 at 142.802 kb on + strand, within HMPREF1058_RS01375 at 142.849 kb on + strand, within HMPREF1058_RS01375 at 142.872 kb on + strand, within HMPREF1058_RS01375 at 142.948 kb on - strand, within HMPREF1058_RS01375 at 142.953 kb on - strand, within HMPREF1058_RS01375 at 142.980 kb on - strand, within HMPREF1058_RS01375 at 143.027 kb on - strand, within HMPREF1058_RS01375 at 143.031 kb on - strand, within HMPREF1058_RS01375 at 143.106 kb on + strand, within HMPREF1058_RS01375 at 143.107 kb on - strand, within HMPREF1058_RS01375 at 143.128 kb on + strand, within HMPREF1058_RS01375 at 143.128 kb on + strand, within HMPREF1058_RS01375 at 143.143 kb on - strand, within HMPREF1058_RS01375
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 20 mM (C) remove 140,276 + +0.9 140,282 + +0.2 140,320 + -0.6 140,381 - HMPREF1058_RS01390 0.13 +1.0 140,402 + HMPREF1058_RS01390 0.15 -0.6 140,578 + HMPREF1058_RS01390 0.33 -2.5 140,590 + HMPREF1058_RS01390 0.34 -0.9 140,591 - HMPREF1058_RS01390 0.34 -0.9 140,591 - HMPREF1058_RS01390 0.34 -2.0 140,597 - +0.7 140,597 - HMPREF1058_RS01390 0.34 -0.7 140,697 + HMPREF1058_RS01390 0.44 +0.0 140,697 + HMPREF1058_RS01390 0.44 -1.2 140,856 - HMPREF1058_RS01390 0.60 -1.6 140,858 - HMPREF1058_RS01390 0.60 -0.7 140,946 - HMPREF1058_RS01390 0.69 +0.5 140,988 + HMPREF1058_RS01390 0.73 -0.3 141,068 - HMPREF1058_RS01390 0.81 -1.4 141,068 - HMPREF1058_RS01390 0.81 +0.9 141,193 + +0.7 141,249 - -1.4 141,418 - HMPREF1058_RS01385 0.19 -3.1 141,418 - HMPREF1058_RS01385 0.19 -2.3 141,537 + HMPREF1058_RS01385 0.32 -2.5 141,544 - HMPREF1058_RS01385 0.32 -1.0 141,555 - HMPREF1058_RS01385 0.34 -1.2 141,621 - HMPREF1058_RS01385 0.41 -1.7 141,717 - HMPREF1058_RS01385 0.51 -2.3 141,759 + HMPREF1058_RS01385 0.56 -2.5 141,759 + HMPREF1058_RS01385 0.56 -2.9 141,772 - HMPREF1058_RS01385 0.57 -1.2 141,789 - HMPREF1058_RS01385 0.59 +0.3 141,795 + HMPREF1058_RS01385 0.59 -1.9 141,934 + HMPREF1058_RS01385 0.74 -0.9 141,935 - HMPREF1058_RS01385 0.74 +0.1 141,935 - HMPREF1058_RS01385 0.74 -1.8 142,042 + HMPREF1058_RS01385 0.86 -1.4 142,053 + HMPREF1058_RS01385 0.87 -2.1 142,053 + HMPREF1058_RS01385 0.87 -1.7 142,063 - HMPREF1058_RS01385 0.88 -1.4 142,095 + -4.1 142,110 - -2.3 142,110 - -1.3 142,139 + -0.5 142,178 + -0.4 142,302 - -0.4 142,412 + +0.0 142,412 + -0.9 142,582 - HMPREF1058_RS01375 0.15 -1.5 142,595 + HMPREF1058_RS01375 0.16 +1.0 142,659 + HMPREF1058_RS01375 0.21 -0.2 142,746 + HMPREF1058_RS01375 0.29 -0.3 142,748 + HMPREF1058_RS01375 0.29 -1.4 142,778 + HMPREF1058_RS01375 0.31 +0.3 142,802 + HMPREF1058_RS01375 0.33 +1.2 142,849 + HMPREF1058_RS01375 0.37 +1.1 142,872 + HMPREF1058_RS01375 0.39 +1.2 142,948 - HMPREF1058_RS01375 0.45 -1.2 142,953 - HMPREF1058_RS01375 0.46 -0.5 142,980 - HMPREF1058_RS01375 0.48 +0.4 143,027 - HMPREF1058_RS01375 0.52 -0.8 143,031 - HMPREF1058_RS01375 0.52 -0.5 143,106 + HMPREF1058_RS01375 0.58 -0.4 143,107 - HMPREF1058_RS01375 0.58 +0.1 143,128 + HMPREF1058_RS01375 0.60 +0.6 143,128 + HMPREF1058_RS01375 0.60 -0.3 143,143 - HMPREF1058_RS01375 0.61 +1.3
Or see this region's nucleotide sequence