Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23890 and EX31_RS23895 are separated by 210 nucleotides EX31_RS23895 and EX31_RS23900 are separated by 17 nucleotides EX31_RS23900 and EX31_RS23905 are separated by 3 nucleotides
EX31_RS23890: EX31_RS23890 - hypothetical protein, at 545,430 to 545,687
_RS23890
EX31_RS23895: EX31_RS23895 - TetR/AcrR family transcriptional regulator, at 545,898 to 546,557
_RS23895
EX31_RS23900: EX31_RS23900 - DUF2236 domain-containing protein, at 546,575 to 547,441
_RS23900
EX31_RS23905: EX31_RS23905 - p-aminobenzoyl-glutamate transporter, at 547,445 to 548,968
_RS23905
Position (kb) 546
547
548 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 545.681 kb on + strand at 545.682 kb on - strand at 545.693 kb on + strand at 545.693 kb on + strand at 545.693 kb on + strand at 545.693 kb on + strand at 545.693 kb on + strand at 545.693 kb on + strand at 545.693 kb on + strand at 545.694 kb on - strand at 545.694 kb on - strand at 545.694 kb on - strand at 545.694 kb on - strand at 545.724 kb on + strand at 545.726 kb on + strand at 545.727 kb on - strand at 545.727 kb on - strand at 545.727 kb on - strand at 545.727 kb on - strand at 545.727 kb on - strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.737 kb on + strand at 545.738 kb on - strand at 545.738 kb on - strand at 545.772 kb on + strand at 545.787 kb on - strand at 545.849 kb on + strand at 545.851 kb on + strand at 545.855 kb on + strand at 545.855 kb on + strand at 545.863 kb on + strand at 545.869 kb on + strand at 545.869 kb on + strand at 545.869 kb on + strand at 545.869 kb on + strand at 545.869 kb on + strand at 545.869 kb on + strand at 545.897 kb on + strand at 545.897 kb on + strand at 545.897 kb on + strand at 545.897 kb on + strand at 545.897 kb on + strand at 545.897 kb on + strand at 546.006 kb on + strand, within EX31_RS23895 at 546.066 kb on + strand, within EX31_RS23895 at 546.066 kb on + strand, within EX31_RS23895 at 546.117 kb on + strand, within EX31_RS23895 at 546.117 kb on + strand, within EX31_RS23895 at 546.117 kb on + strand, within EX31_RS23895 at 546.195 kb on + strand, within EX31_RS23895 at 546.251 kb on + strand, within EX31_RS23895 at 546.251 kb on + strand, within EX31_RS23895 at 546.251 kb on + strand, within EX31_RS23895 at 546.251 kb on + strand, within EX31_RS23895 at 546.251 kb on + strand, within EX31_RS23895 at 546.348 kb on + strand, within EX31_RS23895 at 546.348 kb on + strand, within EX31_RS23895 at 546.414 kb on + strand, within EX31_RS23895 at 546.555 kb on + strand at 546.555 kb on + strand at 546.567 kb on + strand at 546.572 kb on + strand at 546.591 kb on + strand at 546.591 kb on + strand at 546.591 kb on + strand at 546.591 kb on + strand at 546.591 kb on + strand at 546.592 kb on - strand at 546.592 kb on - strand at 546.636 kb on + strand at 546.636 kb on + strand at 546.636 kb on + strand at 546.637 kb on - strand at 546.745 kb on - strand, within EX31_RS23900 at 546.745 kb on - strand, within EX31_RS23900 at 546.811 kb on + strand, within EX31_RS23900 at 546.811 kb on + strand, within EX31_RS23900 at 546.871 kb on + strand, within EX31_RS23900 at 546.901 kb on - strand, within EX31_RS23900 at 546.951 kb on - strand, within EX31_RS23900 at 546.972 kb on + strand, within EX31_RS23900 at 546.973 kb on - strand, within EX31_RS23900 at 547.001 kb on + strand, within EX31_RS23900 at 547.001 kb on + strand, within EX31_RS23900 at 547.067 kb on + strand, within EX31_RS23900 at 547.067 kb on + strand, within EX31_RS23900 at 547.068 kb on - strand, within EX31_RS23900 at 547.068 kb on - strand, within EX31_RS23900 at 547.068 kb on - strand, within EX31_RS23900 at 547.068 kb on - strand, within EX31_RS23900 at 547.070 kb on + strand, within EX31_RS23900 at 547.070 kb on + strand, within EX31_RS23900 at 547.071 kb on - strand, within EX31_RS23900 at 547.137 kb on + strand, within EX31_RS23900 at 547.137 kb on + strand, within EX31_RS23900 at 547.138 kb on - strand, within EX31_RS23900 at 547.145 kb on + strand, within EX31_RS23900 at 547.146 kb on - strand, within EX31_RS23900 at 547.146 kb on - strand, within EX31_RS23900 at 547.146 kb on - strand, within EX31_RS23900 at 547.146 kb on - strand, within EX31_RS23900 at 547.146 kb on - strand, within EX31_RS23900 at 547.146 kb on - strand, within EX31_RS23900 at 547.200 kb on + strand, within EX31_RS23900 at 547.200 kb on + strand, within EX31_RS23900 at 547.281 kb on + strand, within EX31_RS23900 at 547.375 kb on - strand at 547.491 kb on + strand at 547.491 kb on + strand at 547.491 kb on + strand at 547.560 kb on + strand at 547.560 kb on + strand at 547.560 kb on + strand at 547.560 kb on + strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.561 kb on - strand at 547.588 kb on - strand at 547.588 kb on - strand at 547.708 kb on + strand, within EX31_RS23905 at 547.708 kb on + strand, within EX31_RS23905 at 547.708 kb on + strand, within EX31_RS23905 at 547.708 kb on + strand, within EX31_RS23905 at 547.724 kb on + strand, within EX31_RS23905 at 547.725 kb on - strand, within EX31_RS23905 at 547.771 kb on + strand, within EX31_RS23905 at 547.771 kb on + strand, within EX31_RS23905 at 547.772 kb on - strand, within EX31_RS23905 at 547.877 kb on - strand, within EX31_RS23905 at 547.879 kb on + strand, within EX31_RS23905 at 547.880 kb on - strand, within EX31_RS23905 at 547.880 kb on - strand, within EX31_RS23905 at 548.045 kb on - strand, within EX31_RS23905 at 548.057 kb on + strand, within EX31_RS23905 at 548.058 kb on - strand, within EX31_RS23905 at 548.058 kb on - strand, within EX31_RS23905 at 548.269 kb on + strand, within EX31_RS23905 at 548.321 kb on - strand, within EX31_RS23905 at 548.325 kb on + strand, within EX31_RS23905 at 548.325 kb on + strand, within EX31_RS23905 at 548.325 kb on + strand, within EX31_RS23905 at 548.326 kb on - strand, within EX31_RS23905 at 548.326 kb on - strand, within EX31_RS23905 at 548.326 kb on - strand, within EX31_RS23905
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 545,681 + -1.3 545,682 - +1.6 545,693 + +1.0 545,693 + -0.7 545,693 + -0.6 545,693 + -0.5 545,693 + +0.3 545,693 + +0.3 545,693 + +0.5 545,694 - +0.7 545,694 - +1.2 545,694 - -1.2 545,694 - -0.3 545,724 + +1.0 545,726 + +0.0 545,727 - -1.7 545,727 - +0.7 545,727 - -0.1 545,727 - -0.1 545,727 - +0.6 545,737 + +2.0 545,737 + -0.7 545,737 + -1.1 545,737 + -0.5 545,737 + -0.5 545,737 + +0.3 545,737 + +0.0 545,737 + -1.7 545,738 - +0.2 545,738 - -0.1 545,772 + +2.0 545,787 - +0.6 545,849 + +0.5 545,851 + -0.9 545,855 + +0.7 545,855 + +0.6 545,863 + -0.7 545,869 + -1.2 545,869 + +0.6 545,869 + -0.7 545,869 + +0.3 545,869 + +0.6 545,869 + +0.5 545,897 + -2.5 545,897 + +0.6 545,897 + +0.5 545,897 + +0.3 545,897 + -0.3 545,897 + -0.4 546,006 + EX31_RS23895 0.16 -0.4 546,066 + EX31_RS23895 0.25 -1.7 546,066 + EX31_RS23895 0.25 -1.4 546,117 + EX31_RS23895 0.33 -1.5 546,117 + EX31_RS23895 0.33 +0.9 546,117 + EX31_RS23895 0.33 -0.9 546,195 + EX31_RS23895 0.45 -0.2 546,251 + EX31_RS23895 0.53 +0.2 546,251 + EX31_RS23895 0.53 -1.8 546,251 + EX31_RS23895 0.53 -1.1 546,251 + EX31_RS23895 0.53 -1.4 546,251 + EX31_RS23895 0.53 -0.5 546,348 + EX31_RS23895 0.68 -0.2 546,348 + EX31_RS23895 0.68 +0.1 546,414 + EX31_RS23895 0.78 -0.4 546,555 + -0.1 546,555 + -0.3 546,567 + -0.5 546,572 + -0.8 546,591 + +0.4 546,591 + +0.5 546,591 + +1.7 546,591 + -1.4 546,591 + -0.3 546,592 - -0.1 546,592 - -0.5 546,636 + -0.2 546,636 + +0.8 546,636 + -1.1 546,637 - +1.2 546,745 - EX31_RS23900 0.20 +1.0 546,745 - EX31_RS23900 0.20 +0.5 546,811 + EX31_RS23900 0.27 -0.2 546,811 + EX31_RS23900 0.27 +2.6 546,871 + EX31_RS23900 0.34 -0.1 546,901 - EX31_RS23900 0.38 -0.1 546,951 - EX31_RS23900 0.43 -1.7 546,972 + EX31_RS23900 0.46 +2.0 546,973 - EX31_RS23900 0.46 -0.3 547,001 + EX31_RS23900 0.49 +0.1 547,001 + EX31_RS23900 0.49 -0.2 547,067 + EX31_RS23900 0.57 +1.3 547,067 + EX31_RS23900 0.57 +0.5 547,068 - EX31_RS23900 0.57 +0.0 547,068 - EX31_RS23900 0.57 -0.4 547,068 - EX31_RS23900 0.57 -0.3 547,068 - EX31_RS23900 0.57 -1.0 547,070 + EX31_RS23900 0.57 +0.8 547,070 + EX31_RS23900 0.57 -0.1 547,071 - EX31_RS23900 0.57 +0.5 547,137 + EX31_RS23900 0.65 -0.0 547,137 + EX31_RS23900 0.65 -0.4 547,138 - EX31_RS23900 0.65 -0.5 547,145 + EX31_RS23900 0.66 -0.4 547,146 - EX31_RS23900 0.66 +1.5 547,146 - EX31_RS23900 0.66 +1.5 547,146 - EX31_RS23900 0.66 -0.3 547,146 - EX31_RS23900 0.66 +1.1 547,146 - EX31_RS23900 0.66 +1.6 547,146 - EX31_RS23900 0.66 +1.2 547,200 + EX31_RS23900 0.72 -2.0 547,200 + EX31_RS23900 0.72 -0.1 547,281 + EX31_RS23900 0.81 +0.8 547,375 - -1.0 547,491 + +2.5 547,491 + -0.7 547,491 + -0.9 547,560 + -0.2 547,560 + -1.7 547,560 + +0.4 547,560 + -0.3 547,561 - +0.6 547,561 - +0.2 547,561 - -0.6 547,561 - +0.6 547,561 - +0.6 547,561 - -0.6 547,561 - +2.0 547,561 - -1.3 547,588 - +0.2 547,588 - -0.3 547,708 + EX31_RS23905 0.17 -1.0 547,708 + EX31_RS23905 0.17 +0.3 547,708 + EX31_RS23905 0.17 +0.3 547,708 + EX31_RS23905 0.17 -0.1 547,724 + EX31_RS23905 0.18 +0.0 547,725 - EX31_RS23905 0.18 +1.0 547,771 + EX31_RS23905 0.21 +1.5 547,771 + EX31_RS23905 0.21 +0.5 547,772 - EX31_RS23905 0.21 +0.4 547,877 - EX31_RS23905 0.28 +0.0 547,879 + EX31_RS23905 0.28 -2.8 547,880 - EX31_RS23905 0.29 +0.1 547,880 - EX31_RS23905 0.29 -1.8 548,045 - EX31_RS23905 0.39 -1.0 548,057 + EX31_RS23905 0.40 +1.0 548,058 - EX31_RS23905 0.40 -0.2 548,058 - EX31_RS23905 0.40 -0.2 548,269 + EX31_RS23905 0.54 -0.7 548,321 - EX31_RS23905 0.57 +0.9 548,325 + EX31_RS23905 0.58 +0.6 548,325 + EX31_RS23905 0.58 +2.0 548,325 + EX31_RS23905 0.58 -0.8 548,326 - EX31_RS23905 0.58 -0.8 548,326 - EX31_RS23905 0.58 +1.2 548,326 - EX31_RS23905 0.58 -0.3
Or see this region's nucleotide sequence