Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22645 and EX31_RS22650 are separated by 68 nucleotides EX31_RS22650 and EX31_RS22655 are separated by 41 nucleotides
EX31_RS22645: EX31_RS22645 - cysteine--tRNA ligase, at 284,150 to 285,541
_RS22645
EX31_RS22650: EX31_RS22650 - DNA-3-methyladenine glycosylase 2, at 285,610 to 286,482
_RS22650
EX31_RS22655: EX31_RS22655 - diguanylate cyclase, at 286,524 to 288,773
_RS22655
Position (kb) 285
286
287 Strain fitness (log2 ratio) -1
0
1
2 at 285.544 kb on + strand at 285.545 kb on - strand at 285.545 kb on - strand at 285.558 kb on + strand at 285.558 kb on + strand at 285.558 kb on + strand at 285.558 kb on + strand at 285.569 kb on + strand at 285.595 kb on + strand at 285.595 kb on + strand at 285.597 kb on + strand at 285.632 kb on - strand at 285.643 kb on + strand at 285.643 kb on + strand at 285.726 kb on + strand, within EX31_RS22650 at 285.727 kb on - strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.848 kb on + strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.849 kb on - strand, within EX31_RS22650 at 285.941 kb on + strand, within EX31_RS22650 at 285.941 kb on + strand, within EX31_RS22650 at 286.068 kb on + strand, within EX31_RS22650 at 286.185 kb on + strand, within EX31_RS22650 at 286.297 kb on + strand, within EX31_RS22650 at 286.298 kb on - strand, within EX31_RS22650 at 286.348 kb on + strand, within EX31_RS22650 at 286.348 kb on + strand, within EX31_RS22650 at 286.349 kb on - strand, within EX31_RS22650 at 286.430 kb on - strand at 286.455 kb on + strand at 286.547 kb on + strand at 286.547 kb on + strand at 286.547 kb on + strand at 286.547 kb on + strand at 286.547 kb on + strand at 286.547 kb on + strand at 286.547 kb on + strand at 286.548 kb on - strand at 286.580 kb on - strand at 286.580 kb on - strand at 286.583 kb on + strand at 286.583 kb on + strand at 286.584 kb on - strand at 286.584 kb on - strand at 286.584 kb on - strand at 286.618 kb on + strand at 286.618 kb on + strand at 286.618 kb on + strand at 286.619 kb on - strand at 286.619 kb on - strand at 286.645 kb on + strand at 286.646 kb on - strand at 286.646 kb on - strand at 286.800 kb on + strand, within EX31_RS22655 at 286.801 kb on - strand, within EX31_RS22655 at 286.801 kb on - strand, within EX31_RS22655 at 286.935 kb on - strand, within EX31_RS22655 at 287.049 kb on + strand, within EX31_RS22655 at 287.050 kb on - strand, within EX31_RS22655 at 287.050 kb on - strand, within EX31_RS22655 at 287.068 kb on + strand, within EX31_RS22655 at 287.069 kb on - strand, within EX31_RS22655 at 287.069 kb on - strand, within EX31_RS22655 at 287.069 kb on - strand, within EX31_RS22655 at 287.113 kb on - strand, within EX31_RS22655 at 287.274 kb on - strand, within EX31_RS22655 at 287.297 kb on + strand, within EX31_RS22655 at 287.316 kb on + strand, within EX31_RS22655 at 287.393 kb on + strand, within EX31_RS22655 at 287.393 kb on + strand, within EX31_RS22655 at 287.393 kb on + strand, within EX31_RS22655 at 287.441 kb on + strand, within EX31_RS22655 at 287.441 kb on + strand, within EX31_RS22655 at 287.442 kb on - strand, within EX31_RS22655 at 287.442 kb on - strand, within EX31_RS22655
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 285,544 + +0.6 285,545 - +0.5 285,545 - -0.3 285,558 + -0.7 285,558 + +0.5 285,558 + +0.5 285,558 + +1.6 285,569 + +0.6 285,595 + -1.4 285,595 + -1.5 285,597 + -1.0 285,632 - +1.1 285,643 + +0.2 285,643 + +0.4 285,726 + EX31_RS22650 0.13 -0.3 285,727 - EX31_RS22650 0.13 -0.3 285,848 + EX31_RS22650 0.27 -0.9 285,848 + EX31_RS22650 0.27 +0.8 285,848 + EX31_RS22650 0.27 -0.4 285,848 + EX31_RS22650 0.27 +1.4 285,848 + EX31_RS22650 0.27 +0.1 285,848 + EX31_RS22650 0.27 -0.2 285,848 + EX31_RS22650 0.27 -0.2 285,848 + EX31_RS22650 0.27 +1.3 285,848 + EX31_RS22650 0.27 +1.8 285,848 + EX31_RS22650 0.27 -0.2 285,848 + EX31_RS22650 0.27 +0.7 285,848 + EX31_RS22650 0.27 +2.1 285,849 - EX31_RS22650 0.27 +1.7 285,849 - EX31_RS22650 0.27 -0.3 285,849 - EX31_RS22650 0.27 +0.4 285,849 - EX31_RS22650 0.27 -1.3 285,849 - EX31_RS22650 0.27 +0.3 285,849 - EX31_RS22650 0.27 +0.7 285,849 - EX31_RS22650 0.27 -0.3 285,849 - EX31_RS22650 0.27 +0.6 285,849 - EX31_RS22650 0.27 +0.2 285,941 + EX31_RS22650 0.38 -0.1 285,941 + EX31_RS22650 0.38 +0.1 286,068 + EX31_RS22650 0.52 +0.2 286,185 + EX31_RS22650 0.66 -0.1 286,297 + EX31_RS22650 0.79 +1.0 286,298 - EX31_RS22650 0.79 +1.2 286,348 + EX31_RS22650 0.85 +0.9 286,348 + EX31_RS22650 0.85 +1.1 286,349 - EX31_RS22650 0.85 +0.4 286,430 - +1.2 286,455 + +0.5 286,547 + +0.9 286,547 + -0.1 286,547 + -1.1 286,547 + +0.6 286,547 + -0.3 286,547 + +0.7 286,547 + -0.3 286,548 - +0.3 286,580 - +0.4 286,580 - +1.5 286,583 + -0.7 286,583 + +0.3 286,584 - +1.2 286,584 - +1.2 286,584 - +0.1 286,618 + +0.7 286,618 + -1.4 286,618 + +1.1 286,619 - +0.3 286,619 - +0.3 286,645 + +0.6 286,646 - +0.2 286,646 - -1.3 286,800 + EX31_RS22655 0.12 +0.2 286,801 - EX31_RS22655 0.12 +1.6 286,801 - EX31_RS22655 0.12 +1.1 286,935 - EX31_RS22655 0.18 +1.1 287,049 + EX31_RS22655 0.23 -0.0 287,050 - EX31_RS22655 0.23 +0.1 287,050 - EX31_RS22655 0.23 -0.3 287,068 + EX31_RS22655 0.24 +0.1 287,069 - EX31_RS22655 0.24 -0.0 287,069 - EX31_RS22655 0.24 +0.1 287,069 - EX31_RS22655 0.24 -1.0 287,113 - EX31_RS22655 0.26 +1.3 287,274 - EX31_RS22655 0.33 -0.6 287,297 + EX31_RS22655 0.34 +0.1 287,316 + EX31_RS22655 0.35 -0.8 287,393 + EX31_RS22655 0.39 -0.9 287,393 + EX31_RS22655 0.39 +0.8 287,393 + EX31_RS22655 0.39 -0.6 287,441 + EX31_RS22655 0.41 -0.3 287,441 + EX31_RS22655 0.41 -1.4 287,442 - EX31_RS22655 0.41 -1.4 287,442 - EX31_RS22655 0.41 -0.6
Or see this region's nucleotide sequence