Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22560 and EX31_RS22565 are separated by 45 nucleotides EX31_RS22565 and EX31_RS22570 are separated by 276 nucleotides
EX31_RS22560: EX31_RS22560 - MFS transporter, at 266,845 to 268,056
_RS22560
EX31_RS22565: EX31_RS22565 - Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK, at 268,102 to 268,581
_RS22565
EX31_RS22570: EX31_RS22570 - conjugal transfer protein TraB, at 268,858 to 269,664
_RS22570
Position (kb) 268
269 Strain fitness (log2 ratio) -2
-1
0
1
2 at 267.139 kb on + strand, within EX31_RS22560 at 267.144 kb on + strand, within EX31_RS22560 at 267.144 kb on + strand, within EX31_RS22560 at 267.145 kb on - strand, within EX31_RS22560 at 267.145 kb on - strand, within EX31_RS22560 at 267.145 kb on - strand, within EX31_RS22560 at 267.183 kb on - strand, within EX31_RS22560 at 267.183 kb on - strand, within EX31_RS22560 at 267.183 kb on - strand, within EX31_RS22560 at 267.198 kb on + strand, within EX31_RS22560 at 267.198 kb on + strand, within EX31_RS22560 at 267.284 kb on - strand, within EX31_RS22560 at 267.294 kb on + strand, within EX31_RS22560 at 267.295 kb on - strand, within EX31_RS22560 at 267.305 kb on + strand, within EX31_RS22560 at 267.306 kb on - strand, within EX31_RS22560 at 267.315 kb on - strand, within EX31_RS22560 at 267.315 kb on - strand, within EX31_RS22560 at 267.317 kb on + strand, within EX31_RS22560 at 267.317 kb on + strand, within EX31_RS22560 at 267.317 kb on + strand, within EX31_RS22560 at 267.317 kb on + strand, within EX31_RS22560 at 267.318 kb on - strand, within EX31_RS22560 at 267.318 kb on - strand, within EX31_RS22560 at 267.318 kb on - strand, within EX31_RS22560 at 267.339 kb on - strand, within EX31_RS22560 at 267.339 kb on - strand, within EX31_RS22560 at 267.344 kb on + strand, within EX31_RS22560 at 267.345 kb on - strand, within EX31_RS22560 at 267.345 kb on - strand, within EX31_RS22560 at 267.396 kb on + strand, within EX31_RS22560 at 267.465 kb on - strand, within EX31_RS22560 at 267.472 kb on - strand, within EX31_RS22560 at 267.472 kb on - strand, within EX31_RS22560 at 267.768 kb on + strand, within EX31_RS22560 at 267.769 kb on - strand, within EX31_RS22560 at 267.794 kb on + strand, within EX31_RS22560 at 267.794 kb on + strand, within EX31_RS22560 at 267.795 kb on - strand, within EX31_RS22560 at 267.818 kb on + strand, within EX31_RS22560 at 267.818 kb on + strand, within EX31_RS22560 at 267.819 kb on - strand, within EX31_RS22560 at 267.819 kb on - strand, within EX31_RS22560 at 267.860 kb on + strand, within EX31_RS22560 at 267.860 kb on + strand, within EX31_RS22560 at 267.861 kb on - strand, within EX31_RS22560 at 267.935 kb on - strand at 267.957 kb on + strand at 267.957 kb on + strand at 267.957 kb on + strand at 267.958 kb on - strand at 267.958 kb on - strand at 267.990 kb on + strand at 268.056 kb on + strand at 268.057 kb on - strand at 268.057 kb on - strand at 268.075 kb on - strand at 268.075 kb on - strand at 268.075 kb on - strand at 268.075 kb on - strand at 268.075 kb on - strand at 268.079 kb on + strand at 268.079 kb on + strand at 268.080 kb on - strand at 268.080 kb on - strand at 268.080 kb on - strand at 268.080 kb on - strand at 268.142 kb on - strand at 268.142 kb on - strand at 268.202 kb on + strand, within EX31_RS22565 at 268.202 kb on + strand, within EX31_RS22565 at 268.202 kb on + strand, within EX31_RS22565 at 268.202 kb on + strand, within EX31_RS22565 at 268.202 kb on + strand, within EX31_RS22565 at 268.202 kb on + strand, within EX31_RS22565 at 268.203 kb on - strand, within EX31_RS22565 at 268.203 kb on - strand, within EX31_RS22565 at 268.203 kb on - strand, within EX31_RS22565 at 268.203 kb on - strand, within EX31_RS22565 at 268.203 kb on - strand, within EX31_RS22565 at 268.203 kb on - strand, within EX31_RS22565 at 268.209 kb on + strand, within EX31_RS22565 at 268.209 kb on + strand, within EX31_RS22565 at 268.314 kb on + strand, within EX31_RS22565 at 268.315 kb on - strand, within EX31_RS22565 at 268.315 kb on - strand, within EX31_RS22565 at 268.564 kb on + strand at 268.564 kb on + strand at 268.565 kb on - strand at 268.565 kb on - strand at 268.565 kb on - strand at 268.565 kb on - strand at 268.582 kb on - strand at 268.600 kb on + strand at 268.605 kb on + strand at 268.605 kb on + strand at 268.605 kb on + strand at 268.606 kb on - strand at 268.606 kb on - strand at 268.606 kb on - strand at 268.657 kb on + strand at 268.657 kb on + strand at 268.668 kb on + strand at 268.668 kb on + strand at 268.668 kb on + strand at 268.668 kb on + strand at 268.668 kb on + strand at 268.669 kb on - strand at 268.669 kb on - strand at 268.669 kb on - strand at 268.669 kb on - strand at 268.669 kb on - strand at 268.671 kb on + strand at 268.686 kb on + strand at 268.686 kb on + strand at 268.687 kb on - strand at 268.689 kb on + strand at 268.689 kb on + strand at 268.690 kb on - strand at 268.690 kb on - strand at 268.690 kb on - strand at 268.732 kb on - strand at 268.759 kb on + strand at 268.778 kb on + strand at 268.778 kb on + strand at 268.778 kb on + strand at 268.778 kb on + strand at 268.778 kb on + strand at 268.778 kb on + strand at 268.778 kb on + strand at 268.779 kb on - strand at 268.779 kb on - strand at 268.780 kb on + strand at 268.780 kb on + strand at 268.780 kb on + strand at 268.780 kb on + strand at 268.780 kb on + strand at 268.780 kb on + strand at 268.780 kb on + strand at 268.781 kb on - strand at 268.781 kb on - strand at 268.781 kb on - strand at 268.781 kb on - strand at 268.784 kb on + strand at 268.784 kb on + strand at 268.784 kb on + strand at 268.785 kb on - strand at 268.785 kb on - strand at 268.787 kb on + strand at 268.787 kb on + strand at 268.822 kb on + strand at 268.823 kb on - strand at 268.877 kb on - strand at 268.877 kb on - strand at 268.918 kb on + strand at 268.919 kb on - strand at 268.919 kb on - strand at 268.919 kb on - strand at 268.931 kb on + strand at 268.931 kb on + strand at 268.931 kb on + strand at 268.931 kb on + strand at 268.931 kb on + strand at 268.931 kb on + strand at 268.932 kb on - strand at 268.932 kb on - strand at 268.932 kb on - strand at 268.932 kb on - strand at 268.932 kb on - strand at 268.932 kb on - strand at 268.932 kb on - strand at 268.932 kb on - strand at 269.000 kb on + strand, within EX31_RS22570 at 269.000 kb on + strand, within EX31_RS22570 at 269.001 kb on - strand, within EX31_RS22570 at 269.119 kb on - strand, within EX31_RS22570 at 269.161 kb on + strand, within EX31_RS22570 at 269.161 kb on + strand, within EX31_RS22570 at 269.249 kb on + strand, within EX31_RS22570 at 269.250 kb on - strand, within EX31_RS22570 at 269.341 kb on + strand, within EX31_RS22570 at 269.359 kb on + strand, within EX31_RS22570 at 269.538 kb on + strand, within EX31_RS22570
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 267,139 + EX31_RS22560 0.24 +0.8 267,144 + EX31_RS22560 0.25 +0.2 267,144 + EX31_RS22560 0.25 +0.7 267,145 - EX31_RS22560 0.25 +0.7 267,145 - EX31_RS22560 0.25 +0.5 267,145 - EX31_RS22560 0.25 +0.6 267,183 - EX31_RS22560 0.28 -1.9 267,183 - EX31_RS22560 0.28 +0.4 267,183 - EX31_RS22560 0.28 +1.8 267,198 + EX31_RS22560 0.29 -0.3 267,198 + EX31_RS22560 0.29 +1.4 267,284 - EX31_RS22560 0.36 +1.1 267,294 + EX31_RS22560 0.37 -0.0 267,295 - EX31_RS22560 0.37 -0.3 267,305 + EX31_RS22560 0.38 -0.0 267,306 - EX31_RS22560 0.38 -0.1 267,315 - EX31_RS22560 0.39 +1.0 267,315 - EX31_RS22560 0.39 +0.2 267,317 + EX31_RS22560 0.39 +0.3 267,317 + EX31_RS22560 0.39 +0.7 267,317 + EX31_RS22560 0.39 -0.1 267,317 + EX31_RS22560 0.39 -0.9 267,318 - EX31_RS22560 0.39 -0.3 267,318 - EX31_RS22560 0.39 +0.3 267,318 - EX31_RS22560 0.39 +1.2 267,339 - EX31_RS22560 0.41 -1.1 267,339 - EX31_RS22560 0.41 -1.1 267,344 + EX31_RS22560 0.41 -0.7 267,345 - EX31_RS22560 0.41 -1.6 267,345 - EX31_RS22560 0.41 -0.2 267,396 + EX31_RS22560 0.45 -0.8 267,465 - EX31_RS22560 0.51 +0.5 267,472 - EX31_RS22560 0.52 +0.7 267,472 - EX31_RS22560 0.52 +1.8 267,768 + EX31_RS22560 0.76 -0.1 267,769 - EX31_RS22560 0.76 +0.4 267,794 + EX31_RS22560 0.78 -0.9 267,794 + EX31_RS22560 0.78 -0.0 267,795 - EX31_RS22560 0.78 +0.7 267,818 + EX31_RS22560 0.80 +0.9 267,818 + EX31_RS22560 0.80 -1.4 267,819 - EX31_RS22560 0.80 -1.0 267,819 - EX31_RS22560 0.80 +1.3 267,860 + EX31_RS22560 0.84 -0.9 267,860 + EX31_RS22560 0.84 +1.0 267,861 - EX31_RS22560 0.84 +0.4 267,935 - +0.2 267,957 + -0.3 267,957 + +2.1 267,957 + -2.0 267,958 - +0.1 267,958 - +0.8 267,990 + +0.3 268,056 + -0.9 268,057 - -0.2 268,057 - +0.8 268,075 - -0.4 268,075 - +1.3 268,075 - -1.4 268,075 - +0.8 268,075 - +1.1 268,079 + +0.4 268,079 + +0.3 268,080 - -1.9 268,080 - +0.7 268,080 - +0.9 268,080 - +0.6 268,142 - -1.2 268,142 - +0.6 268,202 + EX31_RS22565 0.21 +0.7 268,202 + EX31_RS22565 0.21 -1.5 268,202 + EX31_RS22565 0.21 +1.0 268,202 + EX31_RS22565 0.21 +0.6 268,202 + EX31_RS22565 0.21 +0.3 268,202 + EX31_RS22565 0.21 +1.8 268,203 - EX31_RS22565 0.21 +0.2 268,203 - EX31_RS22565 0.21 +0.6 268,203 - EX31_RS22565 0.21 -0.7 268,203 - EX31_RS22565 0.21 +2.5 268,203 - EX31_RS22565 0.21 -2.3 268,203 - EX31_RS22565 0.21 +0.7 268,209 + EX31_RS22565 0.22 -1.3 268,209 + EX31_RS22565 0.22 +2.7 268,314 + EX31_RS22565 0.44 -0.2 268,315 - EX31_RS22565 0.44 -0.5 268,315 - EX31_RS22565 0.44 +0.3 268,564 + -0.3 268,564 + -0.7 268,565 - +0.2 268,565 - +0.9 268,565 - -0.1 268,565 - +0.7 268,582 - -0.3 268,600 + +0.0 268,605 + -0.9 268,605 + -0.4 268,605 + +0.8 268,606 - -0.7 268,606 - -0.2 268,606 - -0.0 268,657 + -0.0 268,657 + -1.1 268,668 + -1.3 268,668 + -2.7 268,668 + -1.1 268,668 + -2.2 268,668 + -0.5 268,669 - +0.4 268,669 - +0.3 268,669 - -0.7 268,669 - +0.9 268,669 - -0.2 268,671 + +0.7 268,686 + +0.0 268,686 + +0.4 268,687 - +0.3 268,689 + +0.3 268,689 + +1.1 268,690 - +0.6 268,690 - +0.7 268,690 - -0.3 268,732 - -0.2 268,759 + -0.5 268,778 + +0.3 268,778 + -0.2 268,778 + +0.3 268,778 + -0.1 268,778 + -0.0 268,778 + +1.3 268,778 + -0.2 268,779 - -0.5 268,779 - +1.3 268,780 + -0.3 268,780 + -2.3 268,780 + -0.6 268,780 + +0.4 268,780 + +0.1 268,780 + -0.5 268,780 + -0.9 268,781 - -1.6 268,781 - -0.7 268,781 - +0.7 268,781 - +0.6 268,784 + +0.9 268,784 + -0.3 268,784 + -0.5 268,785 - +1.2 268,785 - -0.7 268,787 + -0.3 268,787 + -0.2 268,822 + +0.1 268,823 - -0.1 268,877 - -0.7 268,877 - +0.1 268,918 + +1.9 268,919 - +0.7 268,919 - -1.9 268,919 - +0.0 268,931 + -0.0 268,931 + -0.3 268,931 + +0.4 268,931 + -0.1 268,931 + -0.2 268,931 + -1.0 268,932 - +0.1 268,932 - -0.4 268,932 - -0.5 268,932 - -0.4 268,932 - +0.5 268,932 - +0.6 268,932 - +0.8 268,932 - +0.8 269,000 + EX31_RS22570 0.18 +0.5 269,000 + EX31_RS22570 0.18 -0.3 269,001 - EX31_RS22570 0.18 -1.5 269,119 - EX31_RS22570 0.32 +1.1 269,161 + EX31_RS22570 0.38 -0.4 269,161 + EX31_RS22570 0.38 -1.6 269,249 + EX31_RS22570 0.48 -0.9 269,250 - EX31_RS22570 0.49 +0.4 269,341 + EX31_RS22570 0.60 -0.5 269,359 + EX31_RS22570 0.62 -0.6 269,538 + EX31_RS22570 0.84 -0.1
Or see this region's nucleotide sequence