Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21045 and EX31_RS21050 are separated by 178 nucleotides EX31_RS21050 and EX31_RS21055 are separated by 11 nucleotides EX31_RS21055 and EX31_RS21060 overlap by 4 nucleotides
EX31_RS21045: EX31_RS21045 - S-methyl-5-thioribose-1-phosphate isomerase, at 854,593 to 855,621
_RS21045
EX31_RS21050: EX31_RS21050 - acireductone dioxygenase, at 855,800 to 856,345
_RS21050
EX31_RS21055: EX31_RS21055 - acireductone synthase, at 856,357 to 857,043
_RS21055
EX31_RS21060: EX31_RS21060 - methylthioribulose 1-phosphate dehydratase, at 857,040 to 857,654
_RS21060
Position (kb) 855
856
857 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 854.886 kb on + strand, within EX31_RS21045 at 854.886 kb on + strand, within EX31_RS21045 at 854.886 kb on + strand, within EX31_RS21045 at 854.887 kb on - strand, within EX31_RS21045 at 854.887 kb on - strand, within EX31_RS21045 at 854.914 kb on + strand, within EX31_RS21045 at 854.914 kb on + strand, within EX31_RS21045 at 855.113 kb on + strand, within EX31_RS21045 at 855.113 kb on + strand, within EX31_RS21045 at 855.113 kb on + strand, within EX31_RS21045 at 855.113 kb on + strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.114 kb on - strand, within EX31_RS21045 at 855.179 kb on + strand, within EX31_RS21045 at 855.180 kb on - strand, within EX31_RS21045 at 855.180 kb on - strand, within EX31_RS21045 at 855.193 kb on - strand, within EX31_RS21045 at 855.199 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.319 kb on + strand, within EX31_RS21045 at 855.320 kb on - strand, within EX31_RS21045 at 855.320 kb on - strand, within EX31_RS21045 at 855.344 kb on - strand, within EX31_RS21045 at 855.344 kb on - strand, within EX31_RS21045 at 855.352 kb on - strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.361 kb on + strand, within EX31_RS21045 at 855.362 kb on - strand, within EX31_RS21045 at 855.362 kb on - strand, within EX31_RS21045 at 855.362 kb on - strand, within EX31_RS21045 at 855.362 kb on - strand, within EX31_RS21045 at 855.362 kb on - strand, within EX31_RS21045 at 855.378 kb on + strand, within EX31_RS21045 at 855.379 kb on - strand, within EX31_RS21045 at 855.476 kb on + strand, within EX31_RS21045 at 855.477 kb on - strand, within EX31_RS21045 at 855.477 kb on - strand, within EX31_RS21045 at 855.477 kb on - strand, within EX31_RS21045 at 855.505 kb on + strand, within EX31_RS21045 at 855.505 kb on + strand, within EX31_RS21045 at 855.506 kb on - strand, within EX31_RS21045 at 855.506 kb on - strand, within EX31_RS21045 at 855.506 kb on - strand, within EX31_RS21045 at 855.539 kb on + strand at 855.539 kb on + strand at 855.540 kb on - strand at 855.540 kb on - strand at 855.587 kb on + strand at 855.619 kb on + strand at 855.620 kb on - strand at 855.620 kb on - strand at 855.664 kb on - strand at 855.703 kb on + strand at 855.801 kb on + strand at 855.801 kb on + strand at 855.801 kb on + strand at 855.802 kb on - strand at 855.878 kb on + strand, within EX31_RS21050 at 855.892 kb on + strand, within EX31_RS21050 at 855.892 kb on + strand, within EX31_RS21050 at 855.892 kb on + strand, within EX31_RS21050 at 855.892 kb on + strand, within EX31_RS21050 at 855.913 kb on + strand, within EX31_RS21050 at 855.935 kb on - strand, within EX31_RS21050 at 855.935 kb on - strand, within EX31_RS21050 at 856.054 kb on + strand, within EX31_RS21050 at 856.054 kb on + strand, within EX31_RS21050 at 856.174 kb on - strand, within EX31_RS21050 at 856.174 kb on - strand, within EX31_RS21050 at 856.325 kb on - strand at 856.358 kb on + strand at 856.358 kb on + strand at 856.358 kb on + strand at 856.359 kb on - strand at 856.360 kb on + strand at 856.360 kb on + strand at 856.360 kb on + strand at 856.360 kb on + strand at 856.360 kb on + strand at 856.361 kb on - strand at 856.361 kb on - strand at 856.361 kb on - strand at 856.361 kb on - strand at 856.361 kb on - strand at 856.361 kb on - strand at 856.372 kb on + strand at 856.372 kb on + strand at 856.372 kb on + strand at 856.373 kb on - strand at 856.449 kb on + strand, within EX31_RS21055 at 856.449 kb on + strand, within EX31_RS21055 at 856.449 kb on + strand, within EX31_RS21055 at 856.449 kb on + strand, within EX31_RS21055 at 856.449 kb on + strand, within EX31_RS21055 at 856.450 kb on - strand, within EX31_RS21055 at 856.569 kb on + strand, within EX31_RS21055 at 856.570 kb on - strand, within EX31_RS21055 at 856.570 kb on - strand, within EX31_RS21055 at 856.570 kb on - strand, within EX31_RS21055 at 856.592 kb on + strand, within EX31_RS21055 at 856.592 kb on + strand, within EX31_RS21055 at 856.612 kb on + strand, within EX31_RS21055 at 856.613 kb on - strand, within EX31_RS21055 at 856.733 kb on + strand, within EX31_RS21055 at 856.734 kb on - strand, within EX31_RS21055 at 856.734 kb on - strand, within EX31_RS21055 at 856.734 kb on - strand, within EX31_RS21055 at 856.734 kb on - strand, within EX31_RS21055 at 856.734 kb on - strand, within EX31_RS21055 at 856.829 kb on + strand, within EX31_RS21055 at 856.829 kb on + strand, within EX31_RS21055 at 856.829 kb on + strand, within EX31_RS21055 at 856.829 kb on + strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.830 kb on - strand, within EX31_RS21055 at 856.833 kb on + strand, within EX31_RS21055 at 856.834 kb on - strand, within EX31_RS21055 at 856.891 kb on + strand, within EX31_RS21055 at 856.967 kb on + strand, within EX31_RS21055 at 856.967 kb on + strand, within EX31_RS21055 at 857.020 kb on - strand at 857.045 kb on + strand at 857.226 kb on - strand, within EX31_RS21060 at 857.305 kb on + strand, within EX31_RS21060 at 857.306 kb on - strand, within EX31_RS21060 at 857.306 kb on - strand, within EX31_RS21060 at 857.337 kb on + strand, within EX31_RS21060
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 854,886 + EX31_RS21045 0.28 -0.1 854,886 + EX31_RS21045 0.28 +0.1 854,886 + EX31_RS21045 0.28 -0.2 854,887 - EX31_RS21045 0.29 +1.0 854,887 - EX31_RS21045 0.29 -0.4 854,914 + EX31_RS21045 0.31 -1.0 854,914 + EX31_RS21045 0.31 +0.2 855,113 + EX31_RS21045 0.51 -0.6 855,113 + EX31_RS21045 0.51 +0.0 855,113 + EX31_RS21045 0.51 +0.3 855,113 + EX31_RS21045 0.51 -0.6 855,114 - EX31_RS21045 0.51 +0.6 855,114 - EX31_RS21045 0.51 +0.6 855,114 - EX31_RS21045 0.51 +1.0 855,114 - EX31_RS21045 0.51 -0.1 855,114 - EX31_RS21045 0.51 -0.1 855,114 - EX31_RS21045 0.51 -0.9 855,114 - EX31_RS21045 0.51 +0.5 855,179 + EX31_RS21045 0.57 +0.4 855,180 - EX31_RS21045 0.57 -0.1 855,180 - EX31_RS21045 0.57 +0.2 855,193 - EX31_RS21045 0.58 +1.0 855,199 + EX31_RS21045 0.59 +0.1 855,319 + EX31_RS21045 0.71 -0.6 855,319 + EX31_RS21045 0.71 +0.1 855,319 + EX31_RS21045 0.71 +2.9 855,319 + EX31_RS21045 0.71 -0.4 855,319 + EX31_RS21045 0.71 -0.1 855,319 + EX31_RS21045 0.71 +0.4 855,319 + EX31_RS21045 0.71 +0.8 855,319 + EX31_RS21045 0.71 +1.5 855,320 - EX31_RS21045 0.71 -0.5 855,320 - EX31_RS21045 0.71 +1.5 855,344 - EX31_RS21045 0.73 -1.3 855,344 - EX31_RS21045 0.73 +1.4 855,352 - EX31_RS21045 0.74 -1.0 855,361 + EX31_RS21045 0.75 -0.8 855,361 + EX31_RS21045 0.75 -0.5 855,361 + EX31_RS21045 0.75 -0.5 855,361 + EX31_RS21045 0.75 +1.7 855,361 + EX31_RS21045 0.75 -0.6 855,361 + EX31_RS21045 0.75 +0.0 855,361 + EX31_RS21045 0.75 -0.0 855,361 + EX31_RS21045 0.75 +0.1 855,361 + EX31_RS21045 0.75 +0.6 855,361 + EX31_RS21045 0.75 -1.9 855,361 + EX31_RS21045 0.75 +0.4 855,361 + EX31_RS21045 0.75 -0.1 855,361 + EX31_RS21045 0.75 -2.0 855,362 - EX31_RS21045 0.75 +0.5 855,362 - EX31_RS21045 0.75 +1.0 855,362 - EX31_RS21045 0.75 +0.9 855,362 - EX31_RS21045 0.75 +0.4 855,362 - EX31_RS21045 0.75 +0.2 855,378 + EX31_RS21045 0.76 +0.7 855,379 - EX31_RS21045 0.76 +1.4 855,476 + EX31_RS21045 0.86 -1.5 855,477 - EX31_RS21045 0.86 +0.2 855,477 - EX31_RS21045 0.86 +0.3 855,477 - EX31_RS21045 0.86 -0.7 855,505 + EX31_RS21045 0.89 +0.2 855,505 + EX31_RS21045 0.89 +0.3 855,506 - EX31_RS21045 0.89 +0.4 855,506 - EX31_RS21045 0.89 -2.5 855,506 - EX31_RS21045 0.89 -0.2 855,539 + -1.9 855,539 + -0.0 855,540 - +0.1 855,540 - -0.7 855,587 + +0.6 855,619 + +0.5 855,620 - -0.5 855,620 - +1.8 855,664 - -1.1 855,703 + +0.2 855,801 + -0.6 855,801 + -0.7 855,801 + -0.2 855,802 - +0.5 855,878 + EX31_RS21050 0.14 +1.0 855,892 + EX31_RS21050 0.17 +1.2 855,892 + EX31_RS21050 0.17 +0.7 855,892 + EX31_RS21050 0.17 +0.1 855,892 + EX31_RS21050 0.17 -0.0 855,913 + EX31_RS21050 0.21 +0.6 855,935 - EX31_RS21050 0.25 +0.6 855,935 - EX31_RS21050 0.25 +0.6 856,054 + EX31_RS21050 0.47 +0.5 856,054 + EX31_RS21050 0.47 +0.7 856,174 - EX31_RS21050 0.68 -1.8 856,174 - EX31_RS21050 0.68 +0.5 856,325 - +1.1 856,358 + +0.9 856,358 + -0.7 856,358 + -0.6 856,359 - -1.0 856,360 + +0.2 856,360 + +0.6 856,360 + -0.4 856,360 + +0.8 856,360 + +1.0 856,361 - -0.5 856,361 - +0.6 856,361 - -0.5 856,361 - +0.1 856,361 - -0.2 856,361 - +0.2 856,372 + -1.1 856,372 + +0.5 856,372 + +0.3 856,373 - +0.9 856,449 + EX31_RS21055 0.13 -1.3 856,449 + EX31_RS21055 0.13 -0.0 856,449 + EX31_RS21055 0.13 +0.8 856,449 + EX31_RS21055 0.13 +0.5 856,449 + EX31_RS21055 0.13 -0.3 856,450 - EX31_RS21055 0.14 -0.1 856,569 + EX31_RS21055 0.31 -0.3 856,570 - EX31_RS21055 0.31 +0.6 856,570 - EX31_RS21055 0.31 +0.5 856,570 - EX31_RS21055 0.31 +0.2 856,592 + EX31_RS21055 0.34 -0.5 856,592 + EX31_RS21055 0.34 +0.2 856,612 + EX31_RS21055 0.37 +1.3 856,613 - EX31_RS21055 0.37 +0.5 856,733 + EX31_RS21055 0.55 +1.6 856,734 - EX31_RS21055 0.55 -1.0 856,734 - EX31_RS21055 0.55 -0.3 856,734 - EX31_RS21055 0.55 +0.2 856,734 - EX31_RS21055 0.55 -0.1 856,734 - EX31_RS21055 0.55 -0.6 856,829 + EX31_RS21055 0.69 +1.9 856,829 + EX31_RS21055 0.69 +1.5 856,829 + EX31_RS21055 0.69 -0.2 856,829 + EX31_RS21055 0.69 +0.6 856,830 - EX31_RS21055 0.69 +0.7 856,830 - EX31_RS21055 0.69 -0.5 856,830 - EX31_RS21055 0.69 +0.8 856,830 - EX31_RS21055 0.69 -0.6 856,830 - EX31_RS21055 0.69 -0.7 856,830 - EX31_RS21055 0.69 -0.2 856,830 - EX31_RS21055 0.69 -0.3 856,830 - EX31_RS21055 0.69 +0.2 856,833 + EX31_RS21055 0.69 -1.0 856,834 - EX31_RS21055 0.69 -1.1 856,891 + EX31_RS21055 0.78 +0.9 856,967 + EX31_RS21055 0.89 -1.0 856,967 + EX31_RS21055 0.89 -1.8 857,020 - +0.4 857,045 + -2.7 857,226 - EX31_RS21060 0.30 +0.7 857,305 + EX31_RS21060 0.43 -0.3 857,306 - EX31_RS21060 0.43 -0.2 857,306 - EX31_RS21060 0.43 -0.8 857,337 + EX31_RS21060 0.48 -0.3
Or see this region's nucleotide sequence