Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25010 and EX31_RS20910 are separated by 93 nucleotides EX31_RS20910 and EX31_RS20915 are separated by 17 nucleotides EX31_RS20915 and EX31_RS20920 are separated by 636 nucleotides
EX31_RS25010: EX31_RS25010 - hypothetical protein, at 827,330 to 828,097
_RS25010
EX31_RS20910: EX31_RS20910 - hypothetical protein, at 828,191 to 828,847
_RS20910
EX31_RS20915: EX31_RS20915 - lipoprotein, at 828,865 to 829,419
_RS20915
EX31_RS20920: EX31_RS20920 - hypothetical protein, at 830,056 to 830,433
_RS20920
Position (kb) 828
829
830 Strain fitness (log2 ratio) -2
-1
0
1
2 at 827.906 kb on - strand, within EX31_RS25010 at 828.081 kb on + strand at 828.156 kb on + strand at 828.220 kb on + strand at 828.220 kb on + strand at 828.226 kb on + strand at 828.226 kb on + strand at 828.229 kb on - strand at 828.248 kb on - strand at 828.322 kb on - strand, within EX31_RS20910 at 828.328 kb on + strand, within EX31_RS20910 at 828.331 kb on + strand, within EX31_RS20910 at 828.384 kb on + strand, within EX31_RS20910 at 828.384 kb on + strand, within EX31_RS20910 at 828.384 kb on + strand, within EX31_RS20910 at 828.385 kb on - strand, within EX31_RS20910 at 828.391 kb on + strand, within EX31_RS20910 at 828.472 kb on + strand, within EX31_RS20910 at 828.472 kb on + strand, within EX31_RS20910 at 828.472 kb on + strand, within EX31_RS20910 at 828.472 kb on + strand, within EX31_RS20910 at 828.473 kb on - strand, within EX31_RS20910 at 828.473 kb on - strand, within EX31_RS20910 at 828.475 kb on - strand, within EX31_RS20910 at 828.475 kb on - strand, within EX31_RS20910 at 828.482 kb on - strand, within EX31_RS20910 at 828.580 kb on + strand, within EX31_RS20910 at 828.580 kb on + strand, within EX31_RS20910 at 828.580 kb on + strand, within EX31_RS20910 at 828.580 kb on + strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.581 kb on - strand, within EX31_RS20910 at 828.584 kb on + strand, within EX31_RS20910 at 828.584 kb on + strand, within EX31_RS20910 at 828.585 kb on - strand, within EX31_RS20910 at 828.585 kb on - strand, within EX31_RS20910 at 828.585 kb on - strand, within EX31_RS20910 at 828.635 kb on + strand, within EX31_RS20910 at 828.636 kb on - strand, within EX31_RS20910 at 828.636 kb on - strand, within EX31_RS20910 at 828.647 kb on + strand, within EX31_RS20910 at 828.648 kb on - strand, within EX31_RS20910 at 828.746 kb on + strand, within EX31_RS20910 at 828.747 kb on - strand, within EX31_RS20910 at 828.766 kb on + strand, within EX31_RS20910 at 828.800 kb on + strand at 828.800 kb on + strand at 828.800 kb on + strand at 828.800 kb on + strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.801 kb on - strand at 828.838 kb on + strand at 828.839 kb on - strand at 828.848 kb on - strand at 828.863 kb on - strand at 828.994 kb on - strand, within EX31_RS20915 at 828.994 kb on - strand, within EX31_RS20915 at 828.994 kb on - strand, within EX31_RS20915 at 828.994 kb on - strand, within EX31_RS20915 at 828.995 kb on + strand, within EX31_RS20915 at 828.996 kb on - strand, within EX31_RS20915 at 828.996 kb on - strand, within EX31_RS20915 at 828.996 kb on - strand, within EX31_RS20915 at 828.996 kb on - strand, within EX31_RS20915 at 828.996 kb on - strand, within EX31_RS20915 at 829.149 kb on + strand, within EX31_RS20915 at 829.150 kb on - strand, within EX31_RS20915 at 829.162 kb on - strand, within EX31_RS20915 at 829.208 kb on - strand, within EX31_RS20915 at 829.230 kb on - strand, within EX31_RS20915 at 829.230 kb on - strand, within EX31_RS20915 at 829.276 kb on - strand, within EX31_RS20915 at 829.276 kb on - strand, within EX31_RS20915 at 829.278 kb on + strand, within EX31_RS20915 at 829.278 kb on + strand, within EX31_RS20915 at 829.278 kb on + strand, within EX31_RS20915 at 829.278 kb on + strand, within EX31_RS20915 at 829.279 kb on - strand, within EX31_RS20915 at 829.279 kb on - strand, within EX31_RS20915 at 829.279 kb on - strand, within EX31_RS20915 at 829.282 kb on + strand, within EX31_RS20915 at 829.282 kb on + strand, within EX31_RS20915 at 829.282 kb on + strand, within EX31_RS20915 at 829.308 kb on - strand, within EX31_RS20915 at 829.308 kb on - strand, within EX31_RS20915 at 829.308 kb on - strand, within EX31_RS20915 at 829.308 kb on - strand, within EX31_RS20915 at 829.341 kb on + strand, within EX31_RS20915 at 829.342 kb on - strand, within EX31_RS20915 at 829.342 kb on - strand, within EX31_RS20915 at 829.342 kb on - strand, within EX31_RS20915 at 829.453 kb on + strand at 829.453 kb on + strand at 829.453 kb on + strand at 829.453 kb on + strand at 829.454 kb on - strand at 829.454 kb on - strand at 829.454 kb on - strand at 829.463 kb on + strand at 829.463 kb on + strand at 829.463 kb on + strand at 829.463 kb on + strand at 829.464 kb on - strand at 829.502 kb on - strand at 829.502 kb on - strand at 829.605 kb on - strand at 829.611 kb on + strand at 829.628 kb on - strand at 829.629 kb on + strand at 829.641 kb on - strand at 829.650 kb on - strand at 829.672 kb on + strand at 829.734 kb on + strand at 829.734 kb on + strand at 829.735 kb on - strand at 829.735 kb on - strand at 829.735 kb on - strand at 829.735 kb on - strand at 829.783 kb on + strand at 829.803 kb on + strand at 829.804 kb on - strand at 829.804 kb on - strand at 829.804 kb on - strand at 829.804 kb on - strand at 829.804 kb on - strand at 829.818 kb on + strand at 829.818 kb on + strand at 829.842 kb on + strand at 829.862 kb on + strand at 829.863 kb on - strand at 829.865 kb on + strand at 829.866 kb on - strand at 829.866 kb on - strand at 829.912 kb on + strand at 829.916 kb on + strand at 829.934 kb on + strand at 829.935 kb on - strand at 829.935 kb on - strand at 829.935 kb on - strand at 829.939 kb on + strand at 829.940 kb on - strand at 829.940 kb on - strand at 829.959 kb on + strand at 829.959 kb on + strand at 829.959 kb on + strand at 829.959 kb on + strand at 829.963 kb on + strand at 829.964 kb on - strand at 829.964 kb on - strand at 829.964 kb on - strand at 829.973 kb on + strand at 829.973 kb on + strand at 829.974 kb on - strand at 829.974 kb on - strand at 830.018 kb on - strand at 830.018 kb on - strand at 830.023 kb on + strand at 830.029 kb on + strand at 830.030 kb on - strand at 830.030 kb on - strand at 830.030 kb on - strand at 830.035 kb on + strand at 830.042 kb on + strand at 830.042 kb on + strand at 830.042 kb on + strand at 830.042 kb on + strand at 830.043 kb on - strand at 830.043 kb on - strand at 830.043 kb on - strand at 830.043 kb on - strand at 830.044 kb on + strand at 830.044 kb on + strand at 830.052 kb on + strand at 830.053 kb on - strand at 830.053 kb on - strand at 830.053 kb on - strand at 830.070 kb on + strand at 830.113 kb on + strand, within EX31_RS20920 at 830.113 kb on + strand, within EX31_RS20920 at 830.113 kb on + strand, within EX31_RS20920 at 830.114 kb on - strand, within EX31_RS20920 at 830.114 kb on - strand, within EX31_RS20920 at 830.153 kb on + strand, within EX31_RS20920 at 830.165 kb on + strand, within EX31_RS20920 at 830.165 kb on + strand, within EX31_RS20920 at 830.165 kb on + strand, within EX31_RS20920 at 830.166 kb on - strand, within EX31_RS20920 at 830.166 kb on - strand, within EX31_RS20920 at 830.166 kb on - strand, within EX31_RS20920 at 830.166 kb on - strand, within EX31_RS20920 at 830.169 kb on + strand, within EX31_RS20920 at 830.196 kb on + strand, within EX31_RS20920 at 830.197 kb on - strand, within EX31_RS20920 at 830.197 kb on - strand, within EX31_RS20920 at 830.197 kb on - strand, within EX31_RS20920 at 830.199 kb on + strand, within EX31_RS20920 at 830.258 kb on - strand, within EX31_RS20920 at 830.293 kb on + strand, within EX31_RS20920 at 830.293 kb on + strand, within EX31_RS20920 at 830.293 kb on + strand, within EX31_RS20920 at 830.293 kb on + strand, within EX31_RS20920 at 830.293 kb on + strand, within EX31_RS20920 at 830.293 kb on + strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.294 kb on - strand, within EX31_RS20920 at 830.296 kb on - strand, within EX31_RS20920 at 830.337 kb on + strand, within EX31_RS20920 at 830.338 kb on - strand, within EX31_RS20920 at 830.386 kb on - strand, within EX31_RS20920
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 827,906 - EX31_RS25010 0.75 +0.9 828,081 + -0.6 828,156 + -1.1 828,220 + -0.0 828,220 + +0.2 828,226 + +1.4 828,226 + -0.8 828,229 - +0.1 828,248 - +2.3 828,322 - EX31_RS20910 0.20 +0.6 828,328 + EX31_RS20910 0.21 +0.4 828,331 + EX31_RS20910 0.21 -0.2 828,384 + EX31_RS20910 0.29 +0.1 828,384 + EX31_RS20910 0.29 +0.3 828,384 + EX31_RS20910 0.29 -1.0 828,385 - EX31_RS20910 0.30 -1.0 828,391 + EX31_RS20910 0.30 -0.1 828,472 + EX31_RS20910 0.43 -0.9 828,472 + EX31_RS20910 0.43 +0.0 828,472 + EX31_RS20910 0.43 +0.5 828,472 + EX31_RS20910 0.43 -0.5 828,473 - EX31_RS20910 0.43 +1.5 828,473 - EX31_RS20910 0.43 -0.0 828,475 - EX31_RS20910 0.43 +0.6 828,475 - EX31_RS20910 0.43 +0.6 828,482 - EX31_RS20910 0.44 -0.1 828,580 + EX31_RS20910 0.59 -1.6 828,580 + EX31_RS20910 0.59 -0.4 828,580 + EX31_RS20910 0.59 +0.9 828,580 + EX31_RS20910 0.59 +0.6 828,581 - EX31_RS20910 0.59 -1.4 828,581 - EX31_RS20910 0.59 -0.1 828,581 - EX31_RS20910 0.59 +0.4 828,581 - EX31_RS20910 0.59 -0.6 828,581 - EX31_RS20910 0.59 -0.4 828,581 - EX31_RS20910 0.59 +0.1 828,581 - EX31_RS20910 0.59 +0.0 828,584 + EX31_RS20910 0.60 +0.0 828,584 + EX31_RS20910 0.60 -0.1 828,585 - EX31_RS20910 0.60 +0.2 828,585 - EX31_RS20910 0.60 -1.9 828,585 - EX31_RS20910 0.60 +1.2 828,635 + EX31_RS20910 0.68 +1.8 828,636 - EX31_RS20910 0.68 +1.2 828,636 - EX31_RS20910 0.68 -0.2 828,647 + EX31_RS20910 0.69 -0.1 828,648 - EX31_RS20910 0.70 -0.2 828,746 + EX31_RS20910 0.84 +0.5 828,747 - EX31_RS20910 0.85 +0.0 828,766 + EX31_RS20910 0.88 +0.0 828,800 + -0.3 828,800 + -0.9 828,800 + +0.5 828,800 + -0.1 828,801 - -0.2 828,801 - -0.0 828,801 - -0.6 828,801 - -0.4 828,801 - +0.7 828,801 - -0.5 828,801 - -0.8 828,801 - -1.2 828,801 - -0.8 828,801 - +1.0 828,801 - +0.5 828,801 - +0.9 828,801 - +0.1 828,801 - +0.2 828,838 + +0.6 828,839 - +0.9 828,848 - -0.3 828,863 - +0.9 828,994 - EX31_RS20915 0.23 -1.8 828,994 - EX31_RS20915 0.23 -0.9 828,994 - EX31_RS20915 0.23 -0.7 828,994 - EX31_RS20915 0.23 -0.8 828,995 + EX31_RS20915 0.23 +0.9 828,996 - EX31_RS20915 0.24 +0.5 828,996 - EX31_RS20915 0.24 -2.1 828,996 - EX31_RS20915 0.24 -0.9 828,996 - EX31_RS20915 0.24 -0.5 828,996 - EX31_RS20915 0.24 -0.8 829,149 + EX31_RS20915 0.51 -0.9 829,150 - EX31_RS20915 0.51 +0.6 829,162 - EX31_RS20915 0.54 -0.9 829,208 - EX31_RS20915 0.62 -0.0 829,230 - EX31_RS20915 0.66 -1.0 829,230 - EX31_RS20915 0.66 +0.4 829,276 - EX31_RS20915 0.74 -0.3 829,276 - EX31_RS20915 0.74 +0.2 829,278 + EX31_RS20915 0.74 +0.7 829,278 + EX31_RS20915 0.74 +0.1 829,278 + EX31_RS20915 0.74 +0.4 829,278 + EX31_RS20915 0.74 -0.4 829,279 - EX31_RS20915 0.75 -0.3 829,279 - EX31_RS20915 0.75 -0.1 829,279 - EX31_RS20915 0.75 +1.0 829,282 + EX31_RS20915 0.75 -1.1 829,282 + EX31_RS20915 0.75 +0.9 829,282 + EX31_RS20915 0.75 -0.8 829,308 - EX31_RS20915 0.80 -0.8 829,308 - EX31_RS20915 0.80 -0.7 829,308 - EX31_RS20915 0.80 -0.5 829,308 - EX31_RS20915 0.80 +0.3 829,341 + EX31_RS20915 0.86 +1.7 829,342 - EX31_RS20915 0.86 +0.6 829,342 - EX31_RS20915 0.86 -0.7 829,342 - EX31_RS20915 0.86 +1.2 829,453 + -2.6 829,453 + -1.9 829,453 + -1.1 829,453 + -0.2 829,454 - +1.1 829,454 - +0.1 829,454 - -0.1 829,463 + +0.4 829,463 + -0.6 829,463 + -1.6 829,463 + -0.5 829,464 - -2.2 829,502 - -0.4 829,502 - +0.2 829,605 - -0.0 829,611 + -1.1 829,628 - +1.2 829,629 + +0.6 829,641 - -0.2 829,650 - +0.3 829,672 + -0.1 829,734 + -0.6 829,734 + +0.4 829,735 - +0.8 829,735 - -0.5 829,735 - +0.5 829,735 - +1.0 829,783 + -0.7 829,803 + +0.2 829,804 - +1.2 829,804 - +0.7 829,804 - +0.8 829,804 - -1.2 829,804 - +0.3 829,818 + -0.8 829,818 + +0.3 829,842 + -0.1 829,862 + -0.8 829,863 - +0.0 829,865 + +0.1 829,866 - +0.3 829,866 - -2.0 829,912 + -0.9 829,916 + -0.9 829,934 + +0.7 829,935 - +1.0 829,935 - -0.1 829,935 - +0.0 829,939 + +2.1 829,940 - -0.1 829,940 - +0.2 829,959 + -0.1 829,959 + +0.5 829,959 + -0.5 829,959 + +0.3 829,963 + +1.2 829,964 - +0.4 829,964 - -1.0 829,964 - -0.2 829,973 + -0.1 829,973 + -0.3 829,974 - +0.6 829,974 - -0.6 830,018 - +0.2 830,018 - -0.9 830,023 + +1.6 830,029 + +0.7 830,030 - +1.0 830,030 - +1.1 830,030 - +0.3 830,035 + -0.8 830,042 + -1.3 830,042 + +0.1 830,042 + +0.3 830,042 + +0.0 830,043 - +0.9 830,043 - +0.4 830,043 - +1.3 830,043 - +2.0 830,044 + +0.4 830,044 + +1.7 830,052 + -2.1 830,053 - -2.1 830,053 - +0.6 830,053 - +0.1 830,070 + +0.1 830,113 + EX31_RS20920 0.15 -0.6 830,113 + EX31_RS20920 0.15 +0.7 830,113 + EX31_RS20920 0.15 +0.7 830,114 - EX31_RS20920 0.15 -0.8 830,114 - EX31_RS20920 0.15 +0.3 830,153 + EX31_RS20920 0.26 +0.4 830,165 + EX31_RS20920 0.29 +0.4 830,165 + EX31_RS20920 0.29 -0.1 830,165 + EX31_RS20920 0.29 +1.4 830,166 - EX31_RS20920 0.29 -1.0 830,166 - EX31_RS20920 0.29 -0.0 830,166 - EX31_RS20920 0.29 +0.4 830,166 - EX31_RS20920 0.29 -0.3 830,169 + EX31_RS20920 0.30 -0.5 830,196 + EX31_RS20920 0.37 +0.2 830,197 - EX31_RS20920 0.37 +0.8 830,197 - EX31_RS20920 0.37 +1.4 830,197 - EX31_RS20920 0.37 -0.6 830,199 + EX31_RS20920 0.38 -0.6 830,258 - EX31_RS20920 0.53 -0.1 830,293 + EX31_RS20920 0.63 +0.3 830,293 + EX31_RS20920 0.63 +0.0 830,293 + EX31_RS20920 0.63 +0.4 830,293 + EX31_RS20920 0.63 +0.8 830,293 + EX31_RS20920 0.63 +0.7 830,293 + EX31_RS20920 0.63 -1.8 830,294 - EX31_RS20920 0.63 +0.5 830,294 - EX31_RS20920 0.63 -2.7 830,294 - EX31_RS20920 0.63 +0.6 830,294 - EX31_RS20920 0.63 -2.2 830,294 - EX31_RS20920 0.63 -0.2 830,294 - EX31_RS20920 0.63 +0.6 830,294 - EX31_RS20920 0.63 -0.5 830,294 - EX31_RS20920 0.63 +1.0 830,296 - EX31_RS20920 0.63 -0.1 830,337 + EX31_RS20920 0.74 +0.3 830,338 - EX31_RS20920 0.75 -0.1 830,386 - EX31_RS20920 0.87 +0.5
Or see this region's nucleotide sequence