Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19670 and EX31_RS19675 are separated by 102 nucleotides EX31_RS19675 and EX31_RS19680 are separated by 45 nucleotides EX31_RS19680 and EX31_RS19685 are separated by 8 nucleotides
EX31_RS19670: EX31_RS19670 - elongation factor P hydroxylase, at 566,982 to 567,539
_RS19670
EX31_RS19675: EX31_RS19675 - TSUP family transporter, at 567,642 to 568,442
_RS19675
EX31_RS19680: EX31_RS19680 - penicillin-insensitive murein endopeptidase, at 568,488 to 569,339
_RS19680
EX31_RS19685: EX31_RS19685 - chorismate synthase, at 569,348 to 570,433
_RS19685
Position (kb) 568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 567.511 kb on + strand at 567.511 kb on + strand at 567.511 kb on + strand at 567.511 kb on + strand at 567.511 kb on + strand at 567.511 kb on + strand at 567.511 kb on + strand at 567.511 kb on + strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.512 kb on - strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.515 kb on + strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.516 kb on - strand at 567.539 kb on + strand at 567.539 kb on + strand at 567.539 kb on + strand at 567.539 kb on + strand at 567.539 kb on + strand at 567.540 kb on - strand at 567.540 kb on - strand at 567.540 kb on - strand at 567.540 kb on - strand at 567.540 kb on - strand at 567.581 kb on + strand at 567.581 kb on + strand at 567.581 kb on + strand at 567.581 kb on + strand at 567.582 kb on - strand at 567.582 kb on - strand at 567.582 kb on - strand at 567.583 kb on + strand at 567.583 kb on + strand at 567.583 kb on + strand at 567.583 kb on + strand at 567.583 kb on + strand at 567.584 kb on - strand at 567.584 kb on - strand at 567.584 kb on - strand at 567.584 kb on - strand at 567.584 kb on - strand at 567.645 kb on + strand at 567.645 kb on + strand at 567.646 kb on - strand at 567.646 kb on - strand at 567.646 kb on - strand at 567.646 kb on - strand at 567.658 kb on - strand at 567.691 kb on + strand at 567.692 kb on - strand at 567.770 kb on + strand, within EX31_RS19675 at 567.770 kb on + strand, within EX31_RS19675 at 567.771 kb on - strand, within EX31_RS19675 at 567.771 kb on - strand, within EX31_RS19675 at 567.771 kb on - strand, within EX31_RS19675 at 567.771 kb on - strand, within EX31_RS19675 at 567.771 kb on - strand, within EX31_RS19675 at 567.849 kb on + strand, within EX31_RS19675 at 567.851 kb on + strand, within EX31_RS19675 at 567.852 kb on - strand, within EX31_RS19675 at 567.852 kb on - strand, within EX31_RS19675 at 567.852 kb on - strand, within EX31_RS19675 at 567.852 kb on - strand, within EX31_RS19675 at 567.881 kb on + strand, within EX31_RS19675 at 567.924 kb on - strand, within EX31_RS19675 at 567.933 kb on + strand, within EX31_RS19675 at 567.956 kb on - strand, within EX31_RS19675 at 567.991 kb on + strand, within EX31_RS19675 at 567.991 kb on + strand, within EX31_RS19675 at 567.991 kb on + strand, within EX31_RS19675 at 567.991 kb on + strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 567.992 kb on - strand, within EX31_RS19675 at 568.038 kb on + strand, within EX31_RS19675 at 568.039 kb on - strand, within EX31_RS19675 at 568.039 kb on - strand, within EX31_RS19675 at 568.081 kb on - strand, within EX31_RS19675 at 568.087 kb on + strand, within EX31_RS19675 at 568.155 kb on - strand, within EX31_RS19675 at 568.188 kb on - strand, within EX31_RS19675 at 568.244 kb on - strand, within EX31_RS19675 at 568.283 kb on + strand, within EX31_RS19675 at 568.284 kb on - strand, within EX31_RS19675 at 568.284 kb on - strand, within EX31_RS19675 at 568.284 kb on - strand, within EX31_RS19675 at 568.401 kb on + strand at 568.479 kb on + strand at 568.479 kb on + strand at 568.489 kb on + strand at 568.490 kb on - strand at 568.718 kb on + strand, within EX31_RS19680 at 568.719 kb on - strand, within EX31_RS19680 at 568.719 kb on - strand, within EX31_RS19680 at 568.719 kb on - strand, within EX31_RS19680 at 568.719 kb on - strand, within EX31_RS19680 at 568.792 kb on - strand, within EX31_RS19680 at 568.794 kb on + strand, within EX31_RS19680 at 568.794 kb on + strand, within EX31_RS19680 at 568.795 kb on - strand, within EX31_RS19680 at 568.795 kb on - strand, within EX31_RS19680 at 568.795 kb on - strand, within EX31_RS19680 at 568.795 kb on - strand, within EX31_RS19680 at 568.795 kb on - strand, within EX31_RS19680 at 568.795 kb on - strand, within EX31_RS19680 at 568.868 kb on + strand, within EX31_RS19680 at 568.868 kb on + strand, within EX31_RS19680 at 568.869 kb on - strand, within EX31_RS19680 at 568.892 kb on + strand, within EX31_RS19680 at 568.893 kb on - strand, within EX31_RS19680 at 568.995 kb on - strand, within EX31_RS19680 at 569.111 kb on - strand, within EX31_RS19680 at 569.133 kb on + strand, within EX31_RS19680 at 569.134 kb on - strand, within EX31_RS19680 at 569.134 kb on - strand, within EX31_RS19680 at 569.140 kb on + strand, within EX31_RS19680 at 569.194 kb on + strand, within EX31_RS19680 at 569.194 kb on + strand, within EX31_RS19680 at 569.344 kb on + strand at 569.345 kb on - strand at 569.349 kb on + strand at 569.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 567,511 + -0.2 567,511 + +0.6 567,511 + +1.6 567,511 + -1.4 567,511 + -0.6 567,511 + -0.7 567,511 + +1.3 567,511 + +1.1 567,512 - -0.5 567,512 - -0.4 567,512 - -0.3 567,512 - -1.2 567,512 - +1.5 567,512 - -1.0 567,512 - -0.1 567,512 - -1.3 567,512 - +0.7 567,512 - -2.0 567,512 - -1.0 567,512 - -0.1 567,512 - +0.6 567,512 - +0.3 567,512 - -0.5 567,515 + +0.6 567,515 + +2.2 567,515 + -0.8 567,515 + +1.3 567,515 + -0.4 567,515 + +0.4 567,515 + +0.7 567,515 + +0.7 567,516 - +0.2 567,516 - -0.1 567,516 - -2.2 567,516 - +0.9 567,516 - +0.6 567,516 - +0.9 567,516 - +0.7 567,516 - -2.8 567,516 - -0.0 567,516 - +0.3 567,516 - -0.2 567,516 - +0.3 567,516 - +0.2 567,539 + +1.2 567,539 + -0.6 567,539 + +0.2 567,539 + +0.2 567,539 + +0.4 567,540 - -0.2 567,540 - -0.1 567,540 - -1.3 567,540 - -0.1 567,540 - -0.4 567,581 + -1.2 567,581 + -0.4 567,581 + -0.4 567,581 + +0.9 567,582 - -0.0 567,582 - +3.2 567,582 - -0.5 567,583 + +0.9 567,583 + -0.7 567,583 + +0.2 567,583 + -0.4 567,583 + -1.1 567,584 - -1.2 567,584 - +0.6 567,584 - +1.3 567,584 - +0.4 567,584 - +0.1 567,645 + -0.3 567,645 + -0.3 567,646 - -0.9 567,646 - +0.2 567,646 - -1.0 567,646 - -0.4 567,658 - +1.3 567,691 + +0.7 567,692 - -0.6 567,770 + EX31_RS19675 0.16 +0.6 567,770 + EX31_RS19675 0.16 +0.2 567,771 - EX31_RS19675 0.16 -1.0 567,771 - EX31_RS19675 0.16 +1.5 567,771 - EX31_RS19675 0.16 -0.1 567,771 - EX31_RS19675 0.16 -1.5 567,771 - EX31_RS19675 0.16 -0.5 567,849 + EX31_RS19675 0.26 -0.5 567,851 + EX31_RS19675 0.26 +1.8 567,852 - EX31_RS19675 0.26 +1.6 567,852 - EX31_RS19675 0.26 +0.3 567,852 - EX31_RS19675 0.26 +0.4 567,852 - EX31_RS19675 0.26 -0.4 567,881 + EX31_RS19675 0.30 -1.1 567,924 - EX31_RS19675 0.35 -0.0 567,933 + EX31_RS19675 0.36 +2.2 567,956 - EX31_RS19675 0.39 -1.2 567,991 + EX31_RS19675 0.44 -0.1 567,991 + EX31_RS19675 0.44 +1.0 567,991 + EX31_RS19675 0.44 -0.9 567,991 + EX31_RS19675 0.44 -1.4 567,992 - EX31_RS19675 0.44 -0.8 567,992 - EX31_RS19675 0.44 -0.3 567,992 - EX31_RS19675 0.44 -1.0 567,992 - EX31_RS19675 0.44 -0.6 567,992 - EX31_RS19675 0.44 -0.6 567,992 - EX31_RS19675 0.44 +0.2 567,992 - EX31_RS19675 0.44 -2.8 568,038 + EX31_RS19675 0.49 +1.7 568,039 - EX31_RS19675 0.50 -0.3 568,039 - EX31_RS19675 0.50 -0.6 568,081 - EX31_RS19675 0.55 -0.9 568,087 + EX31_RS19675 0.56 -0.3 568,155 - EX31_RS19675 0.64 -0.7 568,188 - EX31_RS19675 0.68 +0.7 568,244 - EX31_RS19675 0.75 -0.9 568,283 + EX31_RS19675 0.80 -0.7 568,284 - EX31_RS19675 0.80 -0.1 568,284 - EX31_RS19675 0.80 +0.8 568,284 - EX31_RS19675 0.80 +0.6 568,401 + +0.1 568,479 + +0.3 568,479 + +0.3 568,489 + +0.6 568,490 - -0.5 568,718 + EX31_RS19680 0.27 +3.7 568,719 - EX31_RS19680 0.27 -0.0 568,719 - EX31_RS19680 0.27 -0.0 568,719 - EX31_RS19680 0.27 -0.2 568,719 - EX31_RS19680 0.27 +0.4 568,792 - EX31_RS19680 0.36 +0.8 568,794 + EX31_RS19680 0.36 +0.8 568,794 + EX31_RS19680 0.36 +1.5 568,795 - EX31_RS19680 0.36 +0.2 568,795 - EX31_RS19680 0.36 -0.2 568,795 - EX31_RS19680 0.36 +0.4 568,795 - EX31_RS19680 0.36 +0.0 568,795 - EX31_RS19680 0.36 +0.9 568,795 - EX31_RS19680 0.36 +0.8 568,868 + EX31_RS19680 0.45 +0.8 568,868 + EX31_RS19680 0.45 -0.6 568,869 - EX31_RS19680 0.45 +0.8 568,892 + EX31_RS19680 0.47 +0.8 568,893 - EX31_RS19680 0.48 -1.1 568,995 - EX31_RS19680 0.60 +0.7 569,111 - EX31_RS19680 0.73 +0.0 569,133 + EX31_RS19680 0.76 +1.2 569,134 - EX31_RS19680 0.76 +0.6 569,134 - EX31_RS19680 0.76 -1.0 569,140 + EX31_RS19680 0.77 -0.2 569,194 + EX31_RS19680 0.83 +0.1 569,194 + EX31_RS19680 0.83 +0.7 569,344 + -1.2 569,345 - +0.9 569,349 + -0.3 569,350 - +0.7
Or see this region's nucleotide sequence