Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19415 and EX31_RS19420 overlap by 4 nucleotides EX31_RS19420 and EX31_RS19425 are separated by 38 nucleotides EX31_RS19425 and EX31_RS19430 are separated by 89 nucleotides EX31_RS19430 and EX31_RS19435 are separated by 22 nucleotides
EX31_RS19415: EX31_RS19415 - NADH-quinone oxidoreductase subunit NuoF, at 511,368 to 512,729
_RS19415
EX31_RS19420: EX31_RS19420 - NADH-quinone oxidoreductase subunit NuoE, at 512,726 to 513,226
_RS19420
EX31_RS19425: EX31_RS19425 - NADH-quinone oxidoreductase subunit C/D, at 513,265 to 515,064
_RS19425
EX31_RS19430: EX31_RS19430 - NADH-quinone oxidoreductase subunit B, at 515,154 to 515,828
_RS19430
EX31_RS19435: EX31_RS19435 - NADH-quinone oxidoreductase subunit A, at 515,851 to 516,294
_RS19435
Position (kb) 513
514
515
516 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 512.310 kb on - strand, within EX31_RS19415 at 512.421 kb on - strand, within EX31_RS19415 at 512.422 kb on + strand, within EX31_RS19415 at 512.422 kb on + strand, within EX31_RS19415 at 512.422 kb on + strand, within EX31_RS19415 at 512.423 kb on - strand, within EX31_RS19415 at 512.423 kb on - strand, within EX31_RS19415 at 512.423 kb on - strand, within EX31_RS19415 at 512.423 kb on - strand, within EX31_RS19415 at 512.451 kb on - strand, within EX31_RS19415 at 512.721 kb on - strand at 512.722 kb on + strand at 512.722 kb on + strand at 512.722 kb on + strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 512.723 kb on - strand at 513.000 kb on + strand, within EX31_RS19420 at 513.001 kb on - strand, within EX31_RS19420 at 513.001 kb on - strand, within EX31_RS19420 at 513.061 kb on - strand, within EX31_RS19420 at 513.064 kb on + strand, within EX31_RS19420 at 513.065 kb on - strand, within EX31_RS19420 at 513.065 kb on - strand, within EX31_RS19420 at 513.142 kb on - strand, within EX31_RS19420 at 513.142 kb on - strand, within EX31_RS19420 at 513.229 kb on - strand at 513.229 kb on - strand at 513.286 kb on + strand at 513.286 kb on + strand at 513.287 kb on - strand at 513.287 kb on - strand at 513.287 kb on - strand at 513.309 kb on - strand at 513.399 kb on - strand at 513.428 kb on + strand at 513.428 kb on + strand at 513.429 kb on - strand at 513.429 kb on - strand at 513.431 kb on + strand at 513.432 kb on - strand at 513.656 kb on - strand, within EX31_RS19425 at 513.656 kb on - strand, within EX31_RS19425 at 513.678 kb on - strand, within EX31_RS19425 at 513.750 kb on + strand, within EX31_RS19425 at 513.751 kb on - strand, within EX31_RS19425 at 513.751 kb on - strand, within EX31_RS19425 at 513.827 kb on + strand, within EX31_RS19425 at 513.827 kb on + strand, within EX31_RS19425 at 513.887 kb on + strand, within EX31_RS19425 at 513.887 kb on + strand, within EX31_RS19425 at 513.887 kb on + strand, within EX31_RS19425 at 513.888 kb on - strand, within EX31_RS19425 at 514.103 kb on + strand, within EX31_RS19425 at 514.104 kb on - strand, within EX31_RS19425 at 514.123 kb on - strand, within EX31_RS19425 at 514.209 kb on - strand, within EX31_RS19425 at 514.209 kb on - strand, within EX31_RS19425 at 514.209 kb on - strand, within EX31_RS19425 at 514.256 kb on + strand, within EX31_RS19425 at 514.257 kb on - strand, within EX31_RS19425 at 514.257 kb on - strand, within EX31_RS19425 at 514.265 kb on + strand, within EX31_RS19425 at 514.266 kb on - strand, within EX31_RS19425 at 514.266 kb on - strand, within EX31_RS19425 at 514.266 kb on - strand, within EX31_RS19425 at 514.372 kb on - strand, within EX31_RS19425 at 514.402 kb on - strand, within EX31_RS19425 at 514.422 kb on + strand, within EX31_RS19425 at 514.422 kb on + strand, within EX31_RS19425 at 514.422 kb on + strand, within EX31_RS19425 at 514.423 kb on - strand, within EX31_RS19425 at 514.509 kb on + strand, within EX31_RS19425 at 514.558 kb on - strand, within EX31_RS19425 at 514.594 kb on - strand, within EX31_RS19425 at 514.666 kb on - strand, within EX31_RS19425 at 514.770 kb on - strand, within EX31_RS19425 at 514.817 kb on - strand, within EX31_RS19425 at 514.857 kb on - strand, within EX31_RS19425 at 514.884 kb on + strand, within EX31_RS19425 at 514.885 kb on - strand at 514.898 kb on - strand at 514.953 kb on + strand at 514.953 kb on + strand at 515.020 kb on - strand at 515.065 kb on - strand at 515.081 kb on + strand at 515.082 kb on - strand at 515.082 kb on - strand at 515.082 kb on - strand at 515.082 kb on - strand at 515.093 kb on + strand at 515.094 kb on - strand at 515.101 kb on - strand at 515.110 kb on - strand at 515.116 kb on - strand at 515.116 kb on - strand at 515.116 kb on - strand at 515.116 kb on - strand at 515.116 kb on - strand at 515.116 kb on - strand at 515.156 kb on - strand at 515.193 kb on - strand at 515.193 kb on - strand at 515.208 kb on - strand at 515.228 kb on + strand, within EX31_RS19430 at 515.239 kb on + strand, within EX31_RS19430 at 515.322 kb on - strand, within EX31_RS19430 at 515.322 kb on - strand, within EX31_RS19430 at 515.322 kb on - strand, within EX31_RS19430 at 515.322 kb on - strand, within EX31_RS19430 at 515.322 kb on - strand, within EX31_RS19430 at 515.323 kb on + strand, within EX31_RS19430 at 515.323 kb on + strand, within EX31_RS19430 at 515.324 kb on - strand, within EX31_RS19430 at 515.324 kb on - strand, within EX31_RS19430 at 515.324 kb on - strand, within EX31_RS19430 at 515.356 kb on + strand, within EX31_RS19430 at 515.357 kb on - strand, within EX31_RS19430 at 515.357 kb on - strand, within EX31_RS19430 at 515.402 kb on - strand, within EX31_RS19430 at 515.402 kb on - strand, within EX31_RS19430 at 515.405 kb on + strand, within EX31_RS19430 at 515.406 kb on - strand, within EX31_RS19430 at 515.410 kb on + strand, within EX31_RS19430 at 515.410 kb on + strand, within EX31_RS19430 at 515.411 kb on - strand, within EX31_RS19430 at 515.411 kb on - strand, within EX31_RS19430 at 515.476 kb on + strand, within EX31_RS19430 at 515.624 kb on - strand, within EX31_RS19430 at 515.624 kb on - strand, within EX31_RS19430 at 515.819 kb on + strand at 515.820 kb on - strand at 515.821 kb on + strand at 515.822 kb on - strand at 515.822 kb on - strand at 515.822 kb on - strand at 515.832 kb on - strand at 515.853 kb on - strand at 515.871 kb on + strand at 515.874 kb on + strand at 515.875 kb on - strand at 515.893 kb on - strand at 515.893 kb on - strand at 515.893 kb on - strand at 515.949 kb on - strand, within EX31_RS19435 at 515.985 kb on + strand, within EX31_RS19435 at 516.039 kb on - strand, within EX31_RS19435 at 516.044 kb on + strand, within EX31_RS19435 at 516.045 kb on - strand, within EX31_RS19435
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 512,310 - EX31_RS19415 0.69 -0.4 512,421 - EX31_RS19415 0.77 +1.6 512,422 + EX31_RS19415 0.77 -0.7 512,422 + EX31_RS19415 0.77 -0.2 512,422 + EX31_RS19415 0.77 +0.1 512,423 - EX31_RS19415 0.77 +0.3 512,423 - EX31_RS19415 0.77 -0.2 512,423 - EX31_RS19415 0.77 -0.9 512,423 - EX31_RS19415 0.77 +0.3 512,451 - EX31_RS19415 0.80 -1.6 512,721 - -1.7 512,722 + +2.0 512,722 + +0.1 512,722 + -1.3 512,723 - +0.1 512,723 - -0.6 512,723 - -0.4 512,723 - +3.7 512,723 - +2.1 512,723 - -0.7 512,723 - +0.1 512,723 - -0.2 512,723 - +0.1 512,723 - -0.8 512,723 - +1.4 513,000 + EX31_RS19420 0.55 -0.2 513,001 - EX31_RS19420 0.55 +0.4 513,001 - EX31_RS19420 0.55 -0.0 513,061 - EX31_RS19420 0.67 -0.4 513,064 + EX31_RS19420 0.67 +1.1 513,065 - EX31_RS19420 0.68 +0.6 513,065 - EX31_RS19420 0.68 -1.1 513,142 - EX31_RS19420 0.83 -0.1 513,142 - EX31_RS19420 0.83 +2.4 513,229 - +0.6 513,229 - +0.4 513,286 + +1.1 513,286 + +0.1 513,287 - +0.1 513,287 - +2.1 513,287 - -0.4 513,309 - -0.3 513,399 - -0.7 513,428 + +0.6 513,428 + +2.7 513,429 - -0.0 513,429 - -0.2 513,431 + +0.6 513,432 - +1.7 513,656 - EX31_RS19425 0.22 -0.4 513,656 - EX31_RS19425 0.22 -1.2 513,678 - EX31_RS19425 0.23 +1.1 513,750 + EX31_RS19425 0.27 -0.4 513,751 - EX31_RS19425 0.27 -1.0 513,751 - EX31_RS19425 0.27 -0.9 513,827 + EX31_RS19425 0.31 +1.6 513,827 + EX31_RS19425 0.31 -0.8 513,887 + EX31_RS19425 0.35 +2.1 513,887 + EX31_RS19425 0.35 +1.4 513,887 + EX31_RS19425 0.35 +1.4 513,888 - EX31_RS19425 0.35 -0.7 514,103 + EX31_RS19425 0.47 -1.4 514,104 - EX31_RS19425 0.47 +0.6 514,123 - EX31_RS19425 0.48 -0.9 514,209 - EX31_RS19425 0.52 -1.8 514,209 - EX31_RS19425 0.52 +1.1 514,209 - EX31_RS19425 0.52 -0.9 514,256 + EX31_RS19425 0.55 +0.9 514,257 - EX31_RS19425 0.55 -0.7 514,257 - EX31_RS19425 0.55 -0.6 514,265 + EX31_RS19425 0.56 +0.1 514,266 - EX31_RS19425 0.56 +1.7 514,266 - EX31_RS19425 0.56 +2.1 514,266 - EX31_RS19425 0.56 +0.1 514,372 - EX31_RS19425 0.61 +0.1 514,402 - EX31_RS19425 0.63 -0.9 514,422 + EX31_RS19425 0.64 +0.6 514,422 + EX31_RS19425 0.64 -0.8 514,422 + EX31_RS19425 0.64 +0.1 514,423 - EX31_RS19425 0.64 -1.2 514,509 + EX31_RS19425 0.69 +0.6 514,558 - EX31_RS19425 0.72 -0.7 514,594 - EX31_RS19425 0.74 -0.4 514,666 - EX31_RS19425 0.78 +0.6 514,770 - EX31_RS19425 0.84 -0.2 514,817 - EX31_RS19425 0.86 +2.1 514,857 - EX31_RS19425 0.88 -0.9 514,884 + EX31_RS19425 0.90 +1.1 514,885 - -0.1 514,898 - +1.1 514,953 + -0.7 514,953 + +2.1 515,020 - +0.7 515,065 - -0.0 515,081 + +1.4 515,082 - +1.0 515,082 - +1.8 515,082 - +0.1 515,082 - -0.6 515,093 + +0.1 515,094 - +3.7 515,101 - +1.0 515,110 - -0.1 515,116 - +0.2 515,116 - -0.7 515,116 - +0.1 515,116 - +0.4 515,116 - -1.1 515,116 - +0.2 515,156 - +0.6 515,193 - +1.0 515,193 - +1.0 515,208 - +1.6 515,228 + EX31_RS19430 0.11 -0.2 515,239 + EX31_RS19430 0.13 +0.1 515,322 - EX31_RS19430 0.25 +0.1 515,322 - EX31_RS19430 0.25 +0.6 515,322 - EX31_RS19430 0.25 -1.5 515,322 - EX31_RS19430 0.25 +0.6 515,322 - EX31_RS19430 0.25 -0.7 515,323 + EX31_RS19430 0.25 +0.6 515,323 + EX31_RS19430 0.25 -1.2 515,324 - EX31_RS19430 0.25 -0.0 515,324 - EX31_RS19430 0.25 -0.6 515,324 - EX31_RS19430 0.25 -0.2 515,356 + EX31_RS19430 0.30 -1.3 515,357 - EX31_RS19430 0.30 +0.6 515,357 - EX31_RS19430 0.30 +1.3 515,402 - EX31_RS19430 0.37 -0.4 515,402 - EX31_RS19430 0.37 +0.3 515,405 + EX31_RS19430 0.37 +0.1 515,406 - EX31_RS19430 0.37 -0.9 515,410 + EX31_RS19430 0.38 +2.1 515,410 + EX31_RS19430 0.38 -0.4 515,411 - EX31_RS19430 0.38 -0.0 515,411 - EX31_RS19430 0.38 +0.4 515,476 + EX31_RS19430 0.48 -0.9 515,624 - EX31_RS19430 0.70 +0.6 515,624 - EX31_RS19430 0.70 +1.0 515,819 + +2.7 515,820 - -0.4 515,821 + +0.6 515,822 - +0.1 515,822 - -0.7 515,822 - -0.3 515,832 - -0.7 515,853 - -1.2 515,871 + +1.1 515,874 + +1.6 515,875 - -0.4 515,893 - +0.1 515,893 - -0.1 515,893 - -0.5 515,949 - EX31_RS19435 0.22 +1.6 515,985 + EX31_RS19435 0.30 +2.7 516,039 - EX31_RS19435 0.42 +1.1 516,044 + EX31_RS19435 0.43 -0.6 516,045 - EX31_RS19435 0.44 -1.8
Or see this region's nucleotide sequence