Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19185 and EX31_RS19190 are separated by 45 nucleotides EX31_RS19190 and EX31_RS19195 are separated by 27 nucleotides
EX31_RS19185: EX31_RS19185 - MFS transporter, at 432,448 to 433,800
_RS19185
EX31_RS19190: EX31_RS19190 - Bcr/CflA family multidrug efflux MFS transporter, at 433,846 to 435,051
_RS19190
EX31_RS19195: EX31_RS19195 - 16S rRNA pseudouridine(516) synthase RsuA, at 435,079 to 435,786
_RS19195
Position (kb) 433
434
435
436 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 432.868 kb on + strand, within EX31_RS19185 at 432.868 kb on + strand, within EX31_RS19185 at 432.868 kb on + strand, within EX31_RS19185 at 432.868 kb on + strand, within EX31_RS19185 at 432.868 kb on + strand, within EX31_RS19185 at 432.868 kb on + strand, within EX31_RS19185 at 432.933 kb on + strand, within EX31_RS19185 at 432.974 kb on + strand, within EX31_RS19185 at 432.974 kb on + strand, within EX31_RS19185 at 432.975 kb on - strand, within EX31_RS19185 at 433.146 kb on + strand, within EX31_RS19185 at 433.155 kb on + strand, within EX31_RS19185 at 433.176 kb on + strand, within EX31_RS19185 at 433.176 kb on + strand, within EX31_RS19185 at 433.176 kb on + strand, within EX31_RS19185 at 433.177 kb on - strand, within EX31_RS19185 at 433.187 kb on - strand, within EX31_RS19185 at 433.187 kb on - strand, within EX31_RS19185 at 433.201 kb on + strand, within EX31_RS19185 at 433.202 kb on - strand, within EX31_RS19185 at 433.202 kb on - strand, within EX31_RS19185 at 433.202 kb on - strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.209 kb on + strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.210 kb on - strand, within EX31_RS19185 at 433.269 kb on + strand, within EX31_RS19185 at 433.270 kb on - strand, within EX31_RS19185 at 433.270 kb on - strand, within EX31_RS19185 at 433.288 kb on + strand, within EX31_RS19185 at 433.335 kb on + strand, within EX31_RS19185 at 433.353 kb on + strand, within EX31_RS19185 at 433.354 kb on - strand, within EX31_RS19185 at 433.354 kb on - strand, within EX31_RS19185 at 433.354 kb on - strand, within EX31_RS19185 at 433.435 kb on + strand, within EX31_RS19185 at 433.585 kb on + strand, within EX31_RS19185 at 433.585 kb on + strand, within EX31_RS19185 at 433.585 kb on + strand, within EX31_RS19185 at 433.633 kb on + strand, within EX31_RS19185 at 433.634 kb on - strand, within EX31_RS19185 at 433.634 kb on - strand, within EX31_RS19185 at 433.652 kb on + strand, within EX31_RS19185 at 433.744 kb on + strand at 433.745 kb on - strand at 433.745 kb on - strand at 433.798 kb on + strand at 433.798 kb on + strand at 433.799 kb on - strand at 433.805 kb on + strand at 433.805 kb on + strand at 433.805 kb on + strand at 433.805 kb on + strand at 433.806 kb on - strand at 433.823 kb on - strand at 433.823 kb on - strand at 433.848 kb on - strand at 433.848 kb on - strand at 433.848 kb on - strand at 433.892 kb on + strand at 434.032 kb on - strand, within EX31_RS19190 at 434.059 kb on - strand, within EX31_RS19190 at 434.059 kb on - strand, within EX31_RS19190 at 434.076 kb on + strand, within EX31_RS19190 at 434.151 kb on + strand, within EX31_RS19190 at 434.238 kb on + strand, within EX31_RS19190 at 434.291 kb on + strand, within EX31_RS19190 at 434.292 kb on - strand, within EX31_RS19190 at 434.322 kb on + strand, within EX31_RS19190 at 434.322 kb on + strand, within EX31_RS19190 at 434.323 kb on - strand, within EX31_RS19190 at 434.323 kb on - strand, within EX31_RS19190 at 434.323 kb on - strand, within EX31_RS19190 at 434.334 kb on - strand, within EX31_RS19190 at 434.334 kb on - strand, within EX31_RS19190 at 434.334 kb on - strand, within EX31_RS19190 at 434.334 kb on - strand, within EX31_RS19190 at 434.334 kb on - strand, within EX31_RS19190 at 434.334 kb on - strand, within EX31_RS19190 at 434.397 kb on + strand, within EX31_RS19190 at 434.397 kb on + strand, within EX31_RS19190 at 434.398 kb on - strand, within EX31_RS19190 at 434.398 kb on - strand, within EX31_RS19190 at 434.398 kb on - strand, within EX31_RS19190 at 434.398 kb on - strand, within EX31_RS19190 at 434.398 kb on - strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.399 kb on + strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.400 kb on - strand, within EX31_RS19190 at 434.440 kb on - strand, within EX31_RS19190 at 434.499 kb on + strand, within EX31_RS19190 at 434.499 kb on + strand, within EX31_RS19190 at 434.499 kb on + strand, within EX31_RS19190 at 434.500 kb on - strand, within EX31_RS19190 at 434.500 kb on - strand, within EX31_RS19190 at 434.501 kb on + strand, within EX31_RS19190 at 434.501 kb on + strand, within EX31_RS19190 at 434.501 kb on + strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.502 kb on - strand, within EX31_RS19190 at 434.573 kb on - strand, within EX31_RS19190 at 434.677 kb on - strand, within EX31_RS19190 at 434.677 kb on - strand, within EX31_RS19190 at 434.891 kb on + strand, within EX31_RS19190 at 434.891 kb on + strand, within EX31_RS19190 at 434.892 kb on - strand, within EX31_RS19190 at 434.892 kb on - strand, within EX31_RS19190 at 434.892 kb on - strand, within EX31_RS19190 at 434.892 kb on - strand, within EX31_RS19190 at 434.933 kb on + strand at 434.955 kb on + strand at 434.955 kb on + strand at 434.955 kb on + strand at 434.955 kb on + strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.956 kb on - strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.966 kb on + strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.967 kb on - strand at 434.970 kb on + strand at 434.971 kb on - strand at 434.971 kb on - strand at 434.971 kb on - strand at 434.971 kb on - strand at 435.001 kb on - strand at 435.080 kb on + strand at 435.132 kb on - strand at 435.146 kb on - strand at 435.146 kb on - strand at 435.229 kb on + strand, within EX31_RS19195 at 435.230 kb on - strand, within EX31_RS19195 at 435.230 kb on - strand, within EX31_RS19195 at 435.322 kb on - strand, within EX31_RS19195 at 435.393 kb on - strand, within EX31_RS19195 at 435.509 kb on + strand, within EX31_RS19195 at 435.533 kb on + strand, within EX31_RS19195 at 435.534 kb on - strand, within EX31_RS19195 at 435.534 kb on - strand, within EX31_RS19195 at 435.564 kb on + strand, within EX31_RS19195 at 435.565 kb on - strand, within EX31_RS19195 at 435.565 kb on - strand, within EX31_RS19195 at 435.575 kb on + strand, within EX31_RS19195 at 435.575 kb on + strand, within EX31_RS19195 at 435.576 kb on - strand, within EX31_RS19195 at 435.755 kb on - strand at 435.755 kb on - strand at 435.755 kb on - strand at 435.832 kb on + strand at 435.833 kb on - strand at 435.858 kb on + strand at 435.911 kb on + strand at 435.912 kb on - strand at 435.915 kb on - strand at 435.937 kb on - strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.956 kb on + strand at 435.957 kb on - strand at 435.957 kb on - strand at 435.957 kb on - strand at 435.957 kb on - strand at 435.957 kb on - strand at 435.957 kb on - strand at 435.957 kb on - strand at 435.959 kb on + strand at 435.959 kb on + strand at 435.959 kb on + strand at 435.959 kb on + strand at 435.959 kb on + strand at 435.960 kb on - strand at 435.960 kb on - strand at 435.960 kb on - strand at 435.993 kb on - strand at 436.002 kb on - strand at 436.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 432,868 + EX31_RS19185 0.31 -1.0 432,868 + EX31_RS19185 0.31 -1.6 432,868 + EX31_RS19185 0.31 -0.5 432,868 + EX31_RS19185 0.31 +1.1 432,868 + EX31_RS19185 0.31 -1.8 432,868 + EX31_RS19185 0.31 -1.2 432,933 + EX31_RS19185 0.36 +0.3 432,974 + EX31_RS19185 0.39 -0.8 432,974 + EX31_RS19185 0.39 -0.8 432,975 - EX31_RS19185 0.39 +0.4 433,146 + EX31_RS19185 0.52 -0.5 433,155 + EX31_RS19185 0.52 -0.7 433,176 + EX31_RS19185 0.54 -0.3 433,176 + EX31_RS19185 0.54 -0.1 433,176 + EX31_RS19185 0.54 -1.3 433,177 - EX31_RS19185 0.54 -0.9 433,187 - EX31_RS19185 0.55 -1.2 433,187 - EX31_RS19185 0.55 +0.4 433,201 + EX31_RS19185 0.56 +1.3 433,202 - EX31_RS19185 0.56 +0.1 433,202 - EX31_RS19185 0.56 -0.9 433,202 - EX31_RS19185 0.56 -0.2 433,209 + EX31_RS19185 0.56 -1.3 433,209 + EX31_RS19185 0.56 +0.6 433,209 + EX31_RS19185 0.56 +0.9 433,209 + EX31_RS19185 0.56 +0.6 433,209 + EX31_RS19185 0.56 -2.0 433,209 + EX31_RS19185 0.56 +0.9 433,209 + EX31_RS19185 0.56 +0.4 433,210 - EX31_RS19185 0.56 +0.7 433,210 - EX31_RS19185 0.56 -0.5 433,210 - EX31_RS19185 0.56 +0.0 433,210 - EX31_RS19185 0.56 -0.2 433,210 - EX31_RS19185 0.56 -1.7 433,210 - EX31_RS19185 0.56 +0.1 433,210 - EX31_RS19185 0.56 -0.7 433,269 + EX31_RS19185 0.61 +0.7 433,270 - EX31_RS19185 0.61 +1.1 433,270 - EX31_RS19185 0.61 -0.1 433,288 + EX31_RS19185 0.62 -2.3 433,335 + EX31_RS19185 0.66 +0.3 433,353 + EX31_RS19185 0.67 +0.5 433,354 - EX31_RS19185 0.67 +1.2 433,354 - EX31_RS19185 0.67 -0.4 433,354 - EX31_RS19185 0.67 -1.2 433,435 + EX31_RS19185 0.73 -0.7 433,585 + EX31_RS19185 0.84 +0.4 433,585 + EX31_RS19185 0.84 +0.3 433,585 + EX31_RS19185 0.84 +1.0 433,633 + EX31_RS19185 0.88 -0.2 433,634 - EX31_RS19185 0.88 -0.6 433,634 - EX31_RS19185 0.88 +2.1 433,652 + EX31_RS19185 0.89 +0.7 433,744 + +1.1 433,745 - +1.5 433,745 - +1.3 433,798 + +0.6 433,798 + +0.4 433,799 - -0.0 433,805 + +0.4 433,805 + +2.1 433,805 + +2.1 433,805 + -1.8 433,806 - +0.7 433,823 - +0.3 433,823 - +0.8 433,848 - +0.8 433,848 - -0.6 433,848 - +0.4 433,892 + -1.1 434,032 - EX31_RS19190 0.15 -0.7 434,059 - EX31_RS19190 0.18 -0.3 434,059 - EX31_RS19190 0.18 -0.9 434,076 + EX31_RS19190 0.19 +2.1 434,151 + EX31_RS19190 0.25 +0.5 434,238 + EX31_RS19190 0.33 +0.8 434,291 + EX31_RS19190 0.37 -0.2 434,292 - EX31_RS19190 0.37 +1.1 434,322 + EX31_RS19190 0.39 +0.0 434,322 + EX31_RS19190 0.39 -1.7 434,323 - EX31_RS19190 0.40 +0.4 434,323 - EX31_RS19190 0.40 -0.2 434,323 - EX31_RS19190 0.40 -1.3 434,334 - EX31_RS19190 0.40 +0.2 434,334 - EX31_RS19190 0.40 +0.8 434,334 - EX31_RS19190 0.40 +1.5 434,334 - EX31_RS19190 0.40 -0.1 434,334 - EX31_RS19190 0.40 +1.1 434,334 - EX31_RS19190 0.40 +0.1 434,397 + EX31_RS19190 0.46 +0.4 434,397 + EX31_RS19190 0.46 -1.6 434,398 - EX31_RS19190 0.46 -0.2 434,398 - EX31_RS19190 0.46 +0.4 434,398 - EX31_RS19190 0.46 +0.4 434,398 - EX31_RS19190 0.46 -0.4 434,398 - EX31_RS19190 0.46 -0.6 434,399 + EX31_RS19190 0.46 -0.4 434,399 + EX31_RS19190 0.46 -0.2 434,399 + EX31_RS19190 0.46 +0.4 434,399 + EX31_RS19190 0.46 -0.9 434,399 + EX31_RS19190 0.46 +0.4 434,399 + EX31_RS19190 0.46 -0.9 434,399 + EX31_RS19190 0.46 +0.8 434,399 + EX31_RS19190 0.46 +1.4 434,399 + EX31_RS19190 0.46 +0.5 434,399 + EX31_RS19190 0.46 +1.1 434,400 - EX31_RS19190 0.46 -1.5 434,400 - EX31_RS19190 0.46 -0.0 434,400 - EX31_RS19190 0.46 -0.2 434,400 - EX31_RS19190 0.46 +0.1 434,400 - EX31_RS19190 0.46 -0.1 434,400 - EX31_RS19190 0.46 -0.4 434,400 - EX31_RS19190 0.46 +0.5 434,400 - EX31_RS19190 0.46 +1.4 434,400 - EX31_RS19190 0.46 +0.7 434,400 - EX31_RS19190 0.46 -0.9 434,400 - EX31_RS19190 0.46 -0.2 434,440 - EX31_RS19190 0.49 +1.1 434,499 + EX31_RS19190 0.54 +0.3 434,499 + EX31_RS19190 0.54 -0.3 434,499 + EX31_RS19190 0.54 -0.5 434,500 - EX31_RS19190 0.54 -0.4 434,500 - EX31_RS19190 0.54 -0.9 434,501 + EX31_RS19190 0.54 +0.5 434,501 + EX31_RS19190 0.54 -0.6 434,501 + EX31_RS19190 0.54 +1.6 434,502 - EX31_RS19190 0.54 -0.3 434,502 - EX31_RS19190 0.54 -0.5 434,502 - EX31_RS19190 0.54 +0.5 434,502 - EX31_RS19190 0.54 -0.2 434,502 - EX31_RS19190 0.54 +0.1 434,502 - EX31_RS19190 0.54 -1.0 434,502 - EX31_RS19190 0.54 +2.1 434,573 - EX31_RS19190 0.60 +2.1 434,677 - EX31_RS19190 0.69 +1.4 434,677 - EX31_RS19190 0.69 -1.0 434,891 + EX31_RS19190 0.87 -0.1 434,891 + EX31_RS19190 0.87 -0.7 434,892 - EX31_RS19190 0.87 -0.4 434,892 - EX31_RS19190 0.87 -0.7 434,892 - EX31_RS19190 0.87 +0.2 434,892 - EX31_RS19190 0.87 -1.3 434,933 + -0.4 434,955 + +1.1 434,955 + +0.3 434,955 + +1.4 434,955 + -1.2 434,956 - +0.3 434,956 - +1.0 434,956 - +0.3 434,956 - -1.0 434,956 - -0.5 434,956 - -0.0 434,956 - +0.7 434,956 - +1.2 434,966 + +0.1 434,966 + +3.1 434,966 + +0.7 434,966 + +0.1 434,966 + +0.1 434,966 + -0.1 434,966 + -0.2 434,966 + +1.3 434,966 + -0.6 434,966 + +0.4 434,966 + -1.8 434,966 + +0.4 434,967 - -1.0 434,967 - -0.3 434,967 - -0.1 434,967 - +1.2 434,967 - +0.2 434,967 - +0.9 434,967 - -0.7 434,967 - -2.1 434,967 - +0.1 434,967 - +0.4 434,967 - -0.5 434,967 - +0.8 434,967 - +0.5 434,967 - +0.1 434,967 - +1.3 434,967 - +0.4 434,967 - -1.2 434,970 + +0.6 434,971 - -0.1 434,971 - -0.4 434,971 - +0.9 434,971 - +0.6 435,001 - -0.4 435,080 + +2.1 435,132 - -1.6 435,146 - +0.5 435,146 - +0.4 435,229 + EX31_RS19195 0.21 +0.4 435,230 - EX31_RS19195 0.21 +0.0 435,230 - EX31_RS19195 0.21 -0.2 435,322 - EX31_RS19195 0.34 -0.6 435,393 - EX31_RS19195 0.44 -0.6 435,509 + EX31_RS19195 0.61 -1.7 435,533 + EX31_RS19195 0.64 -0.3 435,534 - EX31_RS19195 0.64 +1.0 435,534 - EX31_RS19195 0.64 -1.3 435,564 + EX31_RS19195 0.69 +0.1 435,565 - EX31_RS19195 0.69 -0.4 435,565 - EX31_RS19195 0.69 +1.1 435,575 + EX31_RS19195 0.70 +0.6 435,575 + EX31_RS19195 0.70 -0.3 435,576 - EX31_RS19195 0.70 -0.3 435,755 - -1.3 435,755 - +0.7 435,755 - +1.0 435,832 + +2.5 435,833 - +0.3 435,858 + +0.3 435,911 + -0.4 435,912 - -0.1 435,915 - +0.5 435,937 - +0.1 435,956 + -0.7 435,956 + +0.7 435,956 + -0.0 435,956 + +0.7 435,956 + +1.1 435,956 + +1.4 435,956 + -0.0 435,956 + -0.1 435,957 - -0.7 435,957 - +0.5 435,957 - -0.3 435,957 - +0.2 435,957 - +1.2 435,957 - -0.1 435,957 - -1.0 435,959 + +0.1 435,959 + +1.2 435,959 + +0.0 435,959 + -1.2 435,959 + -0.3 435,960 - +0.1 435,960 - -0.6 435,960 - -1.2 435,993 - -0.2 436,002 - +1.5 436,002 - +0.3
Or see this region's nucleotide sequence