Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18935 and EX31_RS18940 are separated by 55 nucleotides EX31_RS18940 and EX31_RS18945 are separated by 55 nucleotides
EX31_RS18935: EX31_RS18935 - sugar phosphotransferase, at 372,308 to 373,756
_RS18935
EX31_RS18940: EX31_RS18940 - glycosyltransferase family 61 protein, at 373,812 to 374,921
_RS18940
EX31_RS18945: EX31_RS18945 - CatB-related O-acetyltransferase, at 374,977 to 375,621
_RS18945
Position (kb) 373
374
375 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 372.812 kb on + strand, within EX31_RS18935 at 372.813 kb on - strand, within EX31_RS18935 at 372.989 kb on + strand, within EX31_RS18935 at 372.989 kb on + strand, within EX31_RS18935 at 373.010 kb on - strand, within EX31_RS18935 at 373.014 kb on - strand, within EX31_RS18935 at 373.018 kb on - strand, within EX31_RS18935 at 373.198 kb on + strand, within EX31_RS18935 at 373.422 kb on + strand, within EX31_RS18935 at 373.539 kb on + strand, within EX31_RS18935 at 373.681 kb on + strand at 373.769 kb on + strand at 373.811 kb on - strand at 373.896 kb on - strand at 373.896 kb on - strand at 373.902 kb on + strand at 373.991 kb on + strand, within EX31_RS18940 at 373.991 kb on + strand, within EX31_RS18940 at 373.992 kb on - strand, within EX31_RS18940 at 373.996 kb on - strand, within EX31_RS18940 at 374.103 kb on + strand, within EX31_RS18940 at 374.291 kb on + strand, within EX31_RS18940 at 374.352 kb on + strand, within EX31_RS18940 at 374.379 kb on - strand, within EX31_RS18940 at 374.509 kb on + strand, within EX31_RS18940 at 374.646 kb on - strand, within EX31_RS18940 at 374.646 kb on - strand, within EX31_RS18940 at 374.659 kb on + strand, within EX31_RS18940 at 374.675 kb on + strand, within EX31_RS18940 at 374.676 kb on - strand, within EX31_RS18940 at 374.676 kb on - strand, within EX31_RS18940 at 374.689 kb on - strand, within EX31_RS18940 at 374.848 kb on - strand at 374.892 kb on + strand at 374.972 kb on + strand at 374.973 kb on - strand at 375.005 kb on - strand at 375.020 kb on + strand at 375.021 kb on - strand at 375.021 kb on - strand at 375.021 kb on - strand at 375.077 kb on - strand, within EX31_RS18945 at 375.139 kb on + strand, within EX31_RS18945 at 375.155 kb on - strand, within EX31_RS18945 at 375.155 kb on - strand, within EX31_RS18945 at 375.156 kb on + strand, within EX31_RS18945 at 375.259 kb on - strand, within EX31_RS18945 at 375.259 kb on - strand, within EX31_RS18945 at 375.263 kb on - strand, within EX31_RS18945 at 375.277 kb on - strand, within EX31_RS18945 at 375.341 kb on + strand, within EX31_RS18945 at 375.345 kb on + strand, within EX31_RS18945 at 375.436 kb on - strand, within EX31_RS18945 at 375.436 kb on - strand, within EX31_RS18945 at 375.462 kb on - strand, within EX31_RS18945 at 375.462 kb on - strand, within EX31_RS18945 at 375.622 kb on - strand at 375.641 kb on - strand at 375.647 kb on - strand at 375.647 kb on - strand at 375.647 kb on - strand at 375.647 kb on - strand at 375.747 kb on - strand at 375.747 kb on - strand at 375.747 kb on - strand at 375.747 kb on - strand at 375.749 kb on - strand at 375.749 kb on - strand at 375.819 kb on - strand at 375.845 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 372,812 + EX31_RS18935 0.35 -0.4 372,813 - EX31_RS18935 0.35 +1.8 372,989 + EX31_RS18935 0.47 -0.1 372,989 + EX31_RS18935 0.47 -1.4 373,010 - EX31_RS18935 0.48 +0.5 373,014 - EX31_RS18935 0.49 +2.1 373,018 - EX31_RS18935 0.49 -1.2 373,198 + EX31_RS18935 0.61 +0.1 373,422 + EX31_RS18935 0.77 +2.1 373,539 + EX31_RS18935 0.85 +2.4 373,681 + +1.1 373,769 + +0.8 373,811 - -0.2 373,896 - -0.8 373,896 - +1.7 373,902 + -0.1 373,991 + EX31_RS18940 0.16 -0.9 373,991 + EX31_RS18940 0.16 -0.5 373,992 - EX31_RS18940 0.16 +1.3 373,996 - EX31_RS18940 0.17 +0.6 374,103 + EX31_RS18940 0.26 +1.9 374,291 + EX31_RS18940 0.43 +0.4 374,352 + EX31_RS18940 0.49 -0.9 374,379 - EX31_RS18940 0.51 +0.7 374,509 + EX31_RS18940 0.63 +1.7 374,646 - EX31_RS18940 0.75 +1.3 374,646 - EX31_RS18940 0.75 +1.1 374,659 + EX31_RS18940 0.76 +1.1 374,675 + EX31_RS18940 0.78 +1.2 374,676 - EX31_RS18940 0.78 +0.7 374,676 - EX31_RS18940 0.78 -0.6 374,689 - EX31_RS18940 0.79 -0.6 374,848 - -0.1 374,892 + -0.2 374,972 + -0.5 374,973 - +0.1 375,005 - -0.3 375,020 + +2.1 375,021 - -0.3 375,021 - -0.6 375,021 - -2.5 375,077 - EX31_RS18945 0.16 +0.0 375,139 + EX31_RS18945 0.25 +3.1 375,155 - EX31_RS18945 0.28 -0.1 375,155 - EX31_RS18945 0.28 +2.1 375,156 + EX31_RS18945 0.28 -0.2 375,259 - EX31_RS18945 0.44 -0.7 375,259 - EX31_RS18945 0.44 +0.5 375,263 - EX31_RS18945 0.44 +0.6 375,277 - EX31_RS18945 0.47 +1.0 375,341 + EX31_RS18945 0.56 +1.1 375,345 + EX31_RS18945 0.57 -0.5 375,436 - EX31_RS18945 0.71 +0.5 375,436 - EX31_RS18945 0.71 +2.1 375,462 - EX31_RS18945 0.75 +0.7 375,462 - EX31_RS18945 0.75 -0.2 375,622 - -0.2 375,641 - +0.1 375,647 - +0.1 375,647 - -1.0 375,647 - +0.8 375,647 - -1.0 375,747 - -1.0 375,747 - -0.1 375,747 - +1.1 375,747 - +0.7 375,749 - -1.0 375,749 - -0.4 375,819 - -0.8 375,845 - +0.3
Or see this region's nucleotide sequence