Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18655 and EX31_RS18660 are separated by 174 nucleotides EX31_RS18660 and EX31_RS18665 are separated by 158 nucleotides
EX31_RS18655: EX31_RS18655 - arginine ABC transporter substrate-binding protein, at 309,180 to 309,914
_RS18655
EX31_RS18660: EX31_RS18660 - cytidine deaminase, at 310,089 to 310,973
_RS18660
EX31_RS18665: EX31_RS18665 - NAD-dependent malic enzyme, at 311,132 to 312,829
_RS18665
Position (kb) 310
311 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 309.139 kb on - strand at 309.271 kb on - strand, within EX31_RS18655 at 309.287 kb on + strand, within EX31_RS18655 at 309.288 kb on - strand, within EX31_RS18655 at 309.288 kb on - strand, within EX31_RS18655 at 309.344 kb on + strand, within EX31_RS18655 at 309.365 kb on + strand, within EX31_RS18655 at 309.431 kb on + strand, within EX31_RS18655 at 309.431 kb on + strand, within EX31_RS18655 at 309.465 kb on - strand, within EX31_RS18655 at 309.489 kb on + strand, within EX31_RS18655 at 309.489 kb on + strand, within EX31_RS18655 at 309.490 kb on - strand, within EX31_RS18655 at 309.492 kb on + strand, within EX31_RS18655 at 309.540 kb on - strand, within EX31_RS18655 at 309.540 kb on - strand, within EX31_RS18655 at 309.598 kb on - strand, within EX31_RS18655 at 309.598 kb on - strand, within EX31_RS18655 at 309.626 kb on + strand, within EX31_RS18655 at 309.645 kb on + strand, within EX31_RS18655 at 309.645 kb on + strand, within EX31_RS18655 at 309.764 kb on + strand, within EX31_RS18655 at 309.765 kb on - strand, within EX31_RS18655 at 309.777 kb on + strand, within EX31_RS18655 at 309.815 kb on + strand, within EX31_RS18655 at 309.815 kb on + strand, within EX31_RS18655 at 309.815 kb on + strand, within EX31_RS18655 at 309.816 kb on - strand, within EX31_RS18655 at 309.818 kb on + strand, within EX31_RS18655 at 309.867 kb on - strand at 309.965 kb on + strand at 309.979 kb on + strand at 309.979 kb on + strand at 309.980 kb on - strand at 309.998 kb on + strand at 309.999 kb on - strand at 309.999 kb on - strand at 309.999 kb on - strand at 310.001 kb on + strand at 310.001 kb on + strand at 310.002 kb on - strand at 310.002 kb on - strand at 310.002 kb on - strand at 310.002 kb on - strand at 310.049 kb on + strand at 310.050 kb on - strand at 310.065 kb on - strand at 310.088 kb on + strand at 310.210 kb on + strand, within EX31_RS18660 at 310.284 kb on - strand, within EX31_RS18660 at 310.284 kb on - strand, within EX31_RS18660 at 310.289 kb on + strand, within EX31_RS18660 at 310.290 kb on - strand, within EX31_RS18660 at 310.493 kb on + strand, within EX31_RS18660 at 310.494 kb on - strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.638 kb on + strand, within EX31_RS18660 at 310.707 kb on + strand, within EX31_RS18660 at 310.707 kb on + strand, within EX31_RS18660 at 310.707 kb on + strand, within EX31_RS18660 at 310.712 kb on + strand, within EX31_RS18660 at 310.713 kb on - strand, within EX31_RS18660 at 310.713 kb on - strand, within EX31_RS18660 at 310.835 kb on + strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.952 kb on + strand at 310.975 kb on - strand at 310.975 kb on - strand at 310.994 kb on + strand at 310.994 kb on + strand at 310.995 kb on - strand at 311.019 kb on - strand at 311.022 kb on + strand at 311.022 kb on + strand at 311.023 kb on - strand at 311.023 kb on - strand at 311.026 kb on + strand at 311.027 kb on - strand at 311.027 kb on - strand at 311.039 kb on - strand at 311.060 kb on + strand at 311.060 kb on + strand at 311.060 kb on + strand at 311.061 kb on - strand at 311.095 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.166 kb on - strand at 311.166 kb on - strand at 311.228 kb on - strand at 311.357 kb on + strand, within EX31_RS18665 at 311.358 kb on - strand, within EX31_RS18665 at 311.358 kb on - strand, within EX31_RS18665 at 311.396 kb on + strand, within EX31_RS18665 at 311.442 kb on - strand, within EX31_RS18665 at 311.442 kb on - strand, within EX31_RS18665 at 311.486 kb on + strand, within EX31_RS18665 at 311.571 kb on + strand, within EX31_RS18665 at 311.571 kb on + strand, within EX31_RS18665 at 311.666 kb on + strand, within EX31_RS18665 at 311.666 kb on + strand, within EX31_RS18665 at 311.696 kb on + strand, within EX31_RS18665 at 311.696 kb on + strand, within EX31_RS18665 at 311.696 kb on + strand, within EX31_RS18665 at 311.697 kb on - strand, within EX31_RS18665 at 311.697 kb on - strand, within EX31_RS18665 at 311.698 kb on + strand, within EX31_RS18665 at 311.699 kb on - strand, within EX31_RS18665 at 311.742 kb on + strand, within EX31_RS18665 at 311.742 kb on + strand, within EX31_RS18665 at 311.743 kb on - strand, within EX31_RS18665 at 311.759 kb on + strand, within EX31_RS18665 at 311.759 kb on + strand, within EX31_RS18665 at 311.759 kb on + strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.786 kb on - strand, within EX31_RS18665 at 311.971 kb on + strand, within EX31_RS18665
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 309,139 - -1.0 309,271 - EX31_RS18655 0.12 -0.2 309,287 + EX31_RS18655 0.15 -1.5 309,288 - EX31_RS18655 0.15 -0.6 309,288 - EX31_RS18655 0.15 -0.7 309,344 + EX31_RS18655 0.22 +0.5 309,365 + EX31_RS18655 0.25 -2.0 309,431 + EX31_RS18655 0.34 +0.8 309,431 + EX31_RS18655 0.34 -0.0 309,465 - EX31_RS18655 0.39 +3.1 309,489 + EX31_RS18655 0.42 -1.6 309,489 + EX31_RS18655 0.42 -0.1 309,490 - EX31_RS18655 0.42 +0.2 309,492 + EX31_RS18655 0.42 -0.9 309,540 - EX31_RS18655 0.49 -0.5 309,540 - EX31_RS18655 0.49 -1.8 309,598 - EX31_RS18655 0.57 +0.5 309,598 - EX31_RS18655 0.57 -2.0 309,626 + EX31_RS18655 0.61 +1.5 309,645 + EX31_RS18655 0.63 -0.4 309,645 + EX31_RS18655 0.63 +0.9 309,764 + EX31_RS18655 0.79 +1.3 309,765 - EX31_RS18655 0.80 +1.5 309,777 + EX31_RS18655 0.81 -2.0 309,815 + EX31_RS18655 0.86 -1.3 309,815 + EX31_RS18655 0.86 +1.5 309,815 + EX31_RS18655 0.86 -0.3 309,816 - EX31_RS18655 0.87 -0.2 309,818 + EX31_RS18655 0.87 +1.3 309,867 - -0.2 309,965 + -0.1 309,979 + +0.5 309,979 + -1.8 309,980 - -0.1 309,998 + +0.7 309,999 - +0.8 309,999 - -1.3 309,999 - -1.3 310,001 + +1.0 310,001 + +1.2 310,002 - +0.2 310,002 - -0.3 310,002 - +0.7 310,002 - +0.1 310,049 + +0.3 310,050 - -0.7 310,065 - +0.8 310,088 + +1.5 310,210 + EX31_RS18660 0.14 +0.6 310,284 - EX31_RS18660 0.22 -0.3 310,284 - EX31_RS18660 0.22 +0.1 310,289 + EX31_RS18660 0.23 -0.3 310,290 - EX31_RS18660 0.23 +0.2 310,493 + EX31_RS18660 0.46 +0.9 310,494 - EX31_RS18660 0.46 -1.1 310,549 + EX31_RS18660 0.52 +1.5 310,549 + EX31_RS18660 0.52 +1.3 310,549 + EX31_RS18660 0.52 +1.0 310,549 + EX31_RS18660 0.52 +0.2 310,549 + EX31_RS18660 0.52 +0.3 310,549 + EX31_RS18660 0.52 +3.1 310,549 + EX31_RS18660 0.52 -1.3 310,550 - EX31_RS18660 0.52 +1.2 310,550 - EX31_RS18660 0.52 +1.5 310,550 - EX31_RS18660 0.52 +0.5 310,550 - EX31_RS18660 0.52 +1.4 310,550 - EX31_RS18660 0.52 +0.5 310,550 - EX31_RS18660 0.52 -0.4 310,638 + EX31_RS18660 0.62 +1.5 310,707 + EX31_RS18660 0.70 +1.6 310,707 + EX31_RS18660 0.70 +0.5 310,707 + EX31_RS18660 0.70 +0.5 310,712 + EX31_RS18660 0.70 -1.9 310,713 - EX31_RS18660 0.71 +0.5 310,713 - EX31_RS18660 0.71 -0.3 310,835 + EX31_RS18660 0.84 -0.9 310,836 - EX31_RS18660 0.84 -0.3 310,836 - EX31_RS18660 0.84 +0.1 310,836 - EX31_RS18660 0.84 +1.1 310,836 - EX31_RS18660 0.84 -0.0 310,836 - EX31_RS18660 0.84 +0.2 310,952 + +0.7 310,975 - -0.3 310,975 - -0.9 310,994 + +0.5 310,994 + +0.1 310,995 - +0.6 311,019 - -0.7 311,022 + +0.9 311,022 + +0.6 311,023 - +1.5 311,023 - -0.3 311,026 + -0.7 311,027 - +1.2 311,027 - +1.3 311,039 - -0.7 311,060 + -0.8 311,060 + -0.3 311,060 + +0.2 311,061 - -0.1 311,095 + -0.7 311,165 + +0.1 311,165 + +0.8 311,165 + +1.5 311,165 + -0.2 311,165 + +0.1 311,166 - +0.5 311,166 - -0.0 311,228 - -0.7 311,357 + EX31_RS18665 0.13 +0.8 311,358 - EX31_RS18665 0.13 +0.3 311,358 - EX31_RS18665 0.13 +0.3 311,396 + EX31_RS18665 0.16 -0.2 311,442 - EX31_RS18665 0.18 +0.0 311,442 - EX31_RS18665 0.18 +1.2 311,486 + EX31_RS18665 0.21 +0.3 311,571 + EX31_RS18665 0.26 +0.4 311,571 + EX31_RS18665 0.26 +0.9 311,666 + EX31_RS18665 0.31 +0.8 311,666 + EX31_RS18665 0.31 +1.0 311,696 + EX31_RS18665 0.33 -1.0 311,696 + EX31_RS18665 0.33 -0.1 311,696 + EX31_RS18665 0.33 +0.3 311,697 - EX31_RS18665 0.33 +0.3 311,697 - EX31_RS18665 0.33 +0.3 311,698 + EX31_RS18665 0.33 +0.3 311,699 - EX31_RS18665 0.33 -0.0 311,742 + EX31_RS18665 0.36 -0.2 311,742 + EX31_RS18665 0.36 -0.0 311,743 - EX31_RS18665 0.36 +1.1 311,759 + EX31_RS18665 0.37 +0.2 311,759 + EX31_RS18665 0.37 +0.1 311,759 + EX31_RS18665 0.37 -0.5 311,760 - EX31_RS18665 0.37 -0.1 311,760 - EX31_RS18665 0.37 -0.2 311,760 - EX31_RS18665 0.37 -1.5 311,760 - EX31_RS18665 0.37 -1.1 311,760 - EX31_RS18665 0.37 -0.1 311,786 - EX31_RS18665 0.39 +1.9 311,971 + EX31_RS18665 0.49 -0.5
Or see this region's nucleotide sequence