Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17595 and EX31_RS17600 are separated by 4 nucleotides EX31_RS17600 and EX31_RS17605 are separated by 228 nucleotides EX31_RS17605 and EX31_RS17610 are separated by 219 nucleotides EX31_RS17610 and EX31_RS17615 are separated by 44 nucleotides
EX31_RS17595: EX31_RS17595 - chorismate mutase, at 72,617 to 72,913
_RS17595
EX31_RS17600: EX31_RS17600 - LysE family translocator, at 72,918 to 73,457
_RS17600
EX31_RS17605: EX31_RS17605 - pyridoxamine 5'-phosphate oxidase family protein, at 73,686 to 74,309
_RS17605
EX31_RS17610: EX31_RS17610 - antibiotic biosynthesis monooxygenase, at 74,529 to 75,071
_RS17610
EX31_RS17615: EX31_RS17615 - hypothetical protein, at 75,116 to 75,394
_RS17615
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2 at 72.709 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.929 kb on + strand at 72.929 kb on + strand at 72.929 kb on + strand at 72.930 kb on - strand at 72.942 kb on - strand at 73.064 kb on + strand, within EX31_RS17600 at 73.077 kb on + strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.150 kb on + strand, within EX31_RS17600 at 73.196 kb on + strand, within EX31_RS17600 at 73.196 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.329 kb on + strand, within EX31_RS17600 at 73.330 kb on - strand, within EX31_RS17600 at 73.352 kb on + strand, within EX31_RS17600 at 73.352 kb on + strand, within EX31_RS17600 at 73.353 kb on - strand, within EX31_RS17600 at 73.358 kb on + strand, within EX31_RS17600 at 73.358 kb on + strand, within EX31_RS17600 at 73.358 kb on + strand, within EX31_RS17600 at 73.359 kb on - strand, within EX31_RS17600 at 73.359 kb on - strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.456 kb on - strand at 73.456 kb on - strand at 73.471 kb on + strand at 73.471 kb on + strand at 73.471 kb on + strand at 73.472 kb on - strand at 73.472 kb on - strand at 73.472 kb on - strand at 73.475 kb on + strand at 73.475 kb on + strand at 73.475 kb on + strand at 73.476 kb on - strand at 73.496 kb on + strand at 73.496 kb on + strand at 73.496 kb on + strand at 73.497 kb on - strand at 73.497 kb on - strand at 73.497 kb on - strand at 73.498 kb on + strand at 73.498 kb on + strand at 73.499 kb on - strand at 73.499 kb on - strand at 73.505 kb on + strand at 73.505 kb on + strand at 73.505 kb on + strand at 73.505 kb on + strand at 73.506 kb on - strand at 73.540 kb on + strand at 73.579 kb on - strand at 73.595 kb on + strand at 73.596 kb on - strand at 73.596 kb on - strand at 73.599 kb on + strand at 73.600 kb on - strand at 73.600 kb on - strand at 73.600 kb on - strand at 73.600 kb on - strand at 73.639 kb on + strand at 73.639 kb on + strand at 73.643 kb on + strand at 73.643 kb on + strand at 73.709 kb on + strand at 73.709 kb on + strand at 73.709 kb on + strand at 73.718 kb on + strand at 73.805 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.965 kb on - strand, within EX31_RS17605 at 73.966 kb on + strand, within EX31_RS17605 at 73.966 kb on + strand, within EX31_RS17605 at 73.966 kb on + strand, within EX31_RS17605 at 73.969 kb on + strand, within EX31_RS17605 at 73.969 kb on + strand, within EX31_RS17605 at 74.027 kb on + strand, within EX31_RS17605 at 74.052 kb on - strand, within EX31_RS17605 at 74.144 kb on + strand, within EX31_RS17605 at 74.145 kb on - strand, within EX31_RS17605 at 74.232 kb on - strand, within EX31_RS17605 at 74.304 kb on + strand at 74.305 kb on - strand at 74.313 kb on + strand at 74.313 kb on + strand at 74.313 kb on + strand at 74.313 kb on + strand at 74.314 kb on - strand at 74.314 kb on - strand at 74.314 kb on - strand at 74.327 kb on + strand at 74.328 kb on - strand at 74.328 kb on - strand at 74.332 kb on - strand at 74.332 kb on - strand at 74.332 kb on - strand at 74.332 kb on - strand at 74.332 kb on - strand at 74.382 kb on - strand at 74.459 kb on + strand at 74.460 kb on - strand at 74.542 kb on + strand at 74.598 kb on - strand, within EX31_RS17610 at 74.598 kb on - strand, within EX31_RS17610 at 74.599 kb on + strand, within EX31_RS17610 at 74.600 kb on - strand, within EX31_RS17610 at 74.600 kb on - strand, within EX31_RS17610 at 74.630 kb on - strand, within EX31_RS17610 at 74.630 kb on - strand, within EX31_RS17610 at 74.649 kb on + strand, within EX31_RS17610 at 74.651 kb on + strand, within EX31_RS17610 at 74.651 kb on + strand, within EX31_RS17610 at 74.652 kb on - strand, within EX31_RS17610 at 74.652 kb on - strand, within EX31_RS17610 at 74.652 kb on - strand, within EX31_RS17610 at 74.686 kb on - strand, within EX31_RS17610 at 74.698 kb on + strand, within EX31_RS17610 at 74.709 kb on - strand, within EX31_RS17610 at 74.735 kb on - strand, within EX31_RS17610 at 74.855 kb on + strand, within EX31_RS17610 at 74.856 kb on - strand, within EX31_RS17610 at 75.061 kb on - strand at 75.061 kb on - strand at 75.061 kb on - strand at 75.064 kb on + strand at 75.064 kb on + strand at 75.072 kb on - strand at 75.091 kb on + strand at 75.091 kb on + strand at 75.092 kb on - strand at 75.092 kb on - strand at 75.095 kb on + strand at 75.163 kb on - strand, within EX31_RS17615 at 75.184 kb on - strand, within EX31_RS17615 at 75.203 kb on - strand, within EX31_RS17615 at 75.239 kb on - strand, within EX31_RS17615 at 75.303 kb on + strand, within EX31_RS17615 at 75.304 kb on - strand, within EX31_RS17615
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 72,709 + EX31_RS17595 0.31 +0.2 72,786 + EX31_RS17595 0.57 -1.8 72,786 + EX31_RS17595 0.57 -0.5 72,786 + EX31_RS17595 0.57 +1.2 72,786 + EX31_RS17595 0.57 +0.8 72,786 + EX31_RS17595 0.57 +0.6 72,786 + EX31_RS17595 0.57 -0.0 72,787 - EX31_RS17595 0.57 +0.6 72,787 - EX31_RS17595 0.57 +2.0 72,787 - EX31_RS17595 0.57 -0.8 72,787 - EX31_RS17595 0.57 +0.4 72,787 - EX31_RS17595 0.57 -0.3 72,787 - EX31_RS17595 0.57 +0.1 72,787 - EX31_RS17595 0.57 +2.5 72,929 + -0.2 72,929 + +0.2 72,929 + -0.9 72,930 - -0.7 72,942 - +0.2 73,064 + EX31_RS17600 0.27 -2.0 73,077 + EX31_RS17600 0.29 -0.6 73,078 - EX31_RS17600 0.30 -0.3 73,078 - EX31_RS17600 0.30 +0.9 73,078 - EX31_RS17600 0.30 +0.6 73,078 - EX31_RS17600 0.30 -1.5 73,150 + EX31_RS17600 0.43 +0.1 73,196 + EX31_RS17600 0.51 -2.6 73,196 + EX31_RS17600 0.51 +1.0 73,258 + EX31_RS17600 0.63 -0.5 73,258 + EX31_RS17600 0.63 +0.3 73,258 + EX31_RS17600 0.63 +0.8 73,259 - EX31_RS17600 0.63 +2.4 73,259 - EX31_RS17600 0.63 +0.9 73,259 - EX31_RS17600 0.63 +1.5 73,259 - EX31_RS17600 0.63 -0.6 73,329 + EX31_RS17600 0.76 +1.4 73,330 - EX31_RS17600 0.76 -1.2 73,352 + EX31_RS17600 0.80 +0.7 73,352 + EX31_RS17600 0.80 +0.6 73,353 - EX31_RS17600 0.81 +1.3 73,358 + EX31_RS17600 0.81 -1.8 73,358 + EX31_RS17600 0.81 +2.0 73,358 + EX31_RS17600 0.81 -0.0 73,359 - EX31_RS17600 0.82 +1.5 73,359 - EX31_RS17600 0.82 -1.2 73,363 + EX31_RS17600 0.82 +1.4 73,363 + EX31_RS17600 0.82 -0.2 73,363 + EX31_RS17600 0.82 -0.7 73,363 + EX31_RS17600 0.82 +0.9 73,456 - +1.1 73,456 - -1.6 73,471 + +0.9 73,471 + +2.0 73,471 + +0.6 73,472 - -0.9 73,472 - -1.7 73,472 - +1.0 73,475 + -0.6 73,475 + -0.0 73,475 + -0.1 73,476 - +1.5 73,496 + -1.3 73,496 + +0.1 73,496 + +0.8 73,497 - +1.6 73,497 - +1.0 73,497 - +0.4 73,498 + -0.1 73,498 + +0.2 73,499 - -0.4 73,499 - +0.4 73,505 + +1.4 73,505 + +0.3 73,505 + -0.1 73,505 + +0.7 73,506 - +1.0 73,540 + +0.7 73,579 - +0.6 73,595 + +0.6 73,596 - -0.7 73,596 - -0.2 73,599 + -0.4 73,600 - +1.6 73,600 - -0.0 73,600 - +1.0 73,600 - -0.4 73,639 + -0.7 73,639 + +1.4 73,643 + -0.0 73,643 + -1.5 73,709 + -0.7 73,709 + -1.7 73,709 + +1.6 73,718 + +1.0 73,805 + EX31_RS17605 0.19 +1.1 73,893 + EX31_RS17605 0.33 +1.2 73,893 + EX31_RS17605 0.33 -1.3 73,893 + EX31_RS17605 0.33 -0.8 73,893 + EX31_RS17605 0.33 -0.0 73,965 - EX31_RS17605 0.45 -1.3 73,966 + EX31_RS17605 0.45 +1.7 73,966 + EX31_RS17605 0.45 +1.3 73,966 + EX31_RS17605 0.45 +1.0 73,969 + EX31_RS17605 0.45 +0.1 73,969 + EX31_RS17605 0.45 -0.3 74,027 + EX31_RS17605 0.55 -0.5 74,052 - EX31_RS17605 0.59 +2.6 74,144 + EX31_RS17605 0.73 -0.8 74,145 - EX31_RS17605 0.74 +1.5 74,232 - EX31_RS17605 0.88 +0.7 74,304 + -0.1 74,305 - +0.6 74,313 + -0.3 74,313 + -1.4 74,313 + +0.1 74,313 + +1.5 74,314 - +1.4 74,314 - +0.4 74,314 - +0.4 74,327 + +0.8 74,328 - -1.0 74,328 - +0.6 74,332 - +2.0 74,332 - +0.8 74,332 - -0.3 74,332 - +1.7 74,332 - -0.3 74,382 - -0.8 74,459 + +1.3 74,460 - +0.3 74,542 + -0.3 74,598 - EX31_RS17610 0.13 -0.3 74,598 - EX31_RS17610 0.13 +0.2 74,599 + EX31_RS17610 0.13 +1.0 74,600 - EX31_RS17610 0.13 -0.3 74,600 - EX31_RS17610 0.13 +1.7 74,630 - EX31_RS17610 0.19 +1.7 74,630 - EX31_RS17610 0.19 -1.5 74,649 + EX31_RS17610 0.22 +1.0 74,651 + EX31_RS17610 0.22 +1.0 74,651 + EX31_RS17610 0.22 +0.7 74,652 - EX31_RS17610 0.23 -1.6 74,652 - EX31_RS17610 0.23 +0.6 74,652 - EX31_RS17610 0.23 +1.1 74,686 - EX31_RS17610 0.29 -1.3 74,698 + EX31_RS17610 0.31 +0.4 74,709 - EX31_RS17610 0.33 +1.0 74,735 - EX31_RS17610 0.38 -0.0 74,855 + EX31_RS17610 0.60 +2.6 74,856 - EX31_RS17610 0.60 +1.7 75,061 - -0.0 75,061 - +2.0 75,061 - -0.2 75,064 + -1.9 75,064 + -0.8 75,072 - -0.2 75,091 + +0.1 75,091 + -1.0 75,092 - -0.5 75,092 - +0.6 75,095 + -0.3 75,163 - EX31_RS17615 0.17 -0.9 75,184 - EX31_RS17615 0.24 -1.1 75,203 - EX31_RS17615 0.31 +0.6 75,239 - EX31_RS17615 0.44 -1.2 75,303 + EX31_RS17615 0.67 +0.9 75,304 - EX31_RS17615 0.67 -0.8
Or see this region's nucleotide sequence