Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15795 and EX31_RS15800 are separated by 278 nucleotides EX31_RS15800 and EX31_RS15805 are separated by 54 nucleotides EX31_RS15805 and EX31_RS15810 are separated by 19 nucleotides
EX31_RS15795: EX31_RS15795 - NAD-dependent DNA ligase LigB, at 10,408 to 12,105
_RS15795
EX31_RS15800: EX31_RS15800 - guanylate kinase, at 12,384 to 13,007
_RS15800
EX31_RS15805: EX31_RS15805 - DNA-directed RNA polymerase subunit omega, at 13,062 to 13,337
_RS15805
EX31_RS15810: EX31_RS15810 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 13,357 to 15,465
_RS15810
Position (kb) 13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 12.084 kb on + strand at 12.084 kb on + strand at 12.084 kb on + strand at 12.085 kb on - strand at 12.093 kb on + strand at 12.142 kb on - strand at 12.162 kb on + strand at 12.162 kb on + strand at 12.162 kb on + strand at 12.163 kb on - strand at 12.163 kb on - strand at 12.163 kb on - strand at 12.163 kb on - strand at 12.163 kb on - strand at 12.163 kb on - strand at 12.163 kb on - strand at 12.180 kb on + strand at 12.181 kb on - strand at 12.181 kb on - strand at 12.181 kb on - strand at 12.192 kb on + strand at 12.192 kb on + strand at 12.239 kb on + strand at 12.239 kb on + strand at 12.239 kb on + strand at 12.239 kb on + strand at 12.239 kb on + strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.240 kb on - strand at 12.259 kb on - strand at 12.286 kb on + strand at 12.286 kb on + strand at 12.286 kb on + strand at 12.287 kb on - strand at 12.287 kb on - strand at 12.321 kb on + strand at 12.321 kb on + strand at 12.321 kb on + strand at 12.321 kb on + strand at 12.321 kb on + strand at 12.321 kb on + strand at 12.321 kb on + strand at 12.323 kb on + strand at 12.323 kb on + strand at 12.323 kb on + strand at 12.323 kb on + strand at 12.323 kb on + strand at 12.323 kb on + strand at 12.323 kb on + strand at 12.349 kb on + strand at 12.349 kb on + strand at 12.349 kb on + strand at 12.349 kb on + strand at 12.349 kb on + strand at 12.356 kb on + strand at 12.356 kb on + strand at 13.020 kb on + strand at 13.020 kb on + strand at 13.023 kb on + strand at 13.023 kb on + strand at 13.023 kb on + strand at 13.023 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.072 kb on + strand at 13.072 kb on + strand at 13.072 kb on + strand at 13.163 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.335 kb on + strand at 13.335 kb on + strand at 13.360 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 12,084 + -0.9 12,084 + +1.5 12,084 + +0.3 12,085 - +0.2 12,093 + +0.0 12,142 - +0.7 12,162 + +0.5 12,162 + +0.0 12,162 + +0.1 12,163 - -1.1 12,163 - +0.1 12,163 - +0.5 12,163 - -0.1 12,163 - +0.7 12,163 - +1.0 12,163 - -0.4 12,180 + +1.3 12,181 - +0.8 12,181 - -1.8 12,181 - -1.9 12,192 + -0.4 12,192 + +0.6 12,239 + +0.1 12,239 + +0.6 12,239 + +0.3 12,239 + -2.6 12,239 + -2.5 12,240 - -1.1 12,240 - -0.4 12,240 - +0.5 12,240 - +0.1 12,240 - -0.8 12,240 - -0.3 12,240 - +0.4 12,240 - +1.8 12,240 - +0.1 12,240 - +0.2 12,240 - +1.0 12,259 - +1.0 12,286 + +0.4 12,286 + +0.4 12,286 + +0.5 12,287 - -0.6 12,287 - -0.5 12,321 + -0.1 12,321 + -0.7 12,321 + +0.2 12,321 + +0.9 12,321 + +0.3 12,321 + +0.5 12,321 + -0.3 12,323 + -0.0 12,323 + -0.1 12,323 + -0.2 12,323 + +1.1 12,323 + +0.3 12,323 + +2.1 12,323 + +1.0 12,349 + -0.7 12,349 + -1.3 12,349 + -0.8 12,349 + +0.2 12,349 + +0.2 12,356 + +2.5 12,356 + +0.3 13,020 + +0.5 13,020 + -0.1 13,023 + -0.4 13,023 + -0.7 13,023 + -0.1 13,023 + +0.5 13,052 + +1.9 13,052 + -1.5 13,052 + -0.2 13,052 + -1.2 13,052 + -0.2 13,059 + -1.2 13,059 + -1.3 13,059 + -0.9 13,059 + -0.7 13,059 + -2.6 13,059 + +1.7 13,059 + -0.7 13,059 + -1.8 13,059 + +0.1 13,059 + -1.3 13,059 + -0.6 13,059 + +0.3 13,059 + -0.8 13,059 + -0.4 13,059 + -1.1 13,059 + -0.7 13,059 + -0.2 13,059 + -1.4 13,059 + -1.0 13,059 + +0.4 13,059 + -1.5 13,059 + -0.7 13,059 + -0.3 13,059 + -0.7 13,059 + -0.4 13,059 + +0.7 13,059 + -0.9 13,059 + -1.2 13,059 + -0.6 13,059 + -1.3 13,059 + -0.7 13,059 + -0.6 13,059 + +0.5 13,059 + -1.3 13,059 + -0.7 13,059 + +0.1 13,059 + -1.0 13,059 + -0.4 13,059 + -0.6 13,059 + -0.5 13,059 + -1.0 13,059 + -1.2 13,059 + -1.2 13,059 + -1.0 13,059 + +0.4 13,059 + -0.7 13,059 + -0.8 13,059 + -0.9 13,059 + +0.2 13,059 + -0.7 13,059 + +0.0 13,059 + +1.1 13,059 + -0.6 13,059 + -0.1 13,059 + -1.5 13,059 + -0.7 13,059 + -1.0 13,061 + -1.9 13,061 + -0.5 13,061 + -0.4 13,061 + -1.3 13,061 + -2.1 13,061 + +0.5 13,061 + -0.9 13,061 + -3.0 13,061 + -1.6 13,061 + +1.7 13,061 + +0.2 13,061 + -0.6 13,061 + -1.3 13,061 + +1.2 13,061 + +0.8 13,061 + -1.6 13,061 + -2.7 13,061 + -0.5 13,061 + -0.3 13,072 + +0.1 13,072 + -0.7 13,072 + +0.2 13,163 + EX31_RS15805 0.37 -0.4 13,204 + EX31_RS15805 0.51 -2.2 13,204 + EX31_RS15805 0.51 -0.1 13,204 + EX31_RS15805 0.51 +0.2 13,204 + EX31_RS15805 0.51 -0.9 13,204 + EX31_RS15805 0.51 -0.7 13,204 + EX31_RS15805 0.51 -1.1 13,204 + EX31_RS15805 0.51 -0.0 13,335 + +0.1 13,335 + -0.5 13,360 + -0.8
Or see this region's nucleotide sequence