Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12450 and EX31_RS12455 overlap by 4 nucleotides EX31_RS12455 and EX31_RS12460 are separated by 168 nucleotides EX31_RS12460 and EX31_RS12465 are separated by 67 nucleotides
EX31_RS12450: EX31_RS12450 - phospholipid-binding protein MlaC, at 156,817 to 157,452
_RS12450
EX31_RS12455: EX31_RS12455 - lipid asymmetry maintenance protein MlaB, at 157,449 to 157,763
_RS12455
EX31_RS12460: EX31_RS12460 - BolA family transcriptional regulator, at 157,932 to 158,186
_RS12460
EX31_RS12465: EX31_RS12465 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 158,254 to 159,513
_RS12465
Position (kb) 157
158
159 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 156.975 kb on + strand, within EX31_RS12450 at 156.975 kb on + strand, within EX31_RS12450 at 156.975 kb on + strand, within EX31_RS12450 at 156.975 kb on + strand, within EX31_RS12450 at 156.975 kb on + strand, within EX31_RS12450 at 156.975 kb on + strand, within EX31_RS12450 at 156.976 kb on - strand, within EX31_RS12450 at 156.976 kb on - strand, within EX31_RS12450 at 156.979 kb on + strand, within EX31_RS12450 at 156.979 kb on + strand, within EX31_RS12450 at 156.979 kb on + strand, within EX31_RS12450 at 156.979 kb on + strand, within EX31_RS12450 at 156.980 kb on - strand, within EX31_RS12450 at 156.980 kb on - strand, within EX31_RS12450 at 156.980 kb on - strand, within EX31_RS12450 at 156.980 kb on - strand, within EX31_RS12450 at 157.015 kb on + strand, within EX31_RS12450 at 157.015 kb on + strand, within EX31_RS12450 at 157.016 kb on - strand, within EX31_RS12450 at 157.016 kb on - strand, within EX31_RS12450 at 157.031 kb on - strand, within EX31_RS12450 at 157.031 kb on - strand, within EX31_RS12450 at 157.049 kb on + strand, within EX31_RS12450 at 157.050 kb on - strand, within EX31_RS12450 at 157.058 kb on - strand, within EX31_RS12450 at 157.060 kb on + strand, within EX31_RS12450 at 157.060 kb on + strand, within EX31_RS12450 at 157.060 kb on + strand, within EX31_RS12450 at 157.061 kb on - strand, within EX31_RS12450 at 157.093 kb on + strand, within EX31_RS12450 at 157.093 kb on + strand, within EX31_RS12450 at 157.093 kb on + strand, within EX31_RS12450 at 157.094 kb on - strand, within EX31_RS12450 at 157.094 kb on - strand, within EX31_RS12450 at 157.129 kb on + strand, within EX31_RS12450 at 157.150 kb on + strand, within EX31_RS12450 at 157.150 kb on + strand, within EX31_RS12450 at 157.150 kb on + strand, within EX31_RS12450 at 157.150 kb on + strand, within EX31_RS12450 at 157.151 kb on - strand, within EX31_RS12450 at 157.151 kb on - strand, within EX31_RS12450 at 157.151 kb on - strand, within EX31_RS12450 at 157.151 kb on - strand, within EX31_RS12450 at 157.151 kb on - strand, within EX31_RS12450 at 157.151 kb on - strand, within EX31_RS12450 at 157.165 kb on + strand, within EX31_RS12450 at 157.166 kb on - strand, within EX31_RS12450 at 157.166 kb on - strand, within EX31_RS12450 at 157.166 kb on - strand, within EX31_RS12450 at 157.166 kb on - strand, within EX31_RS12450 at 157.190 kb on - strand, within EX31_RS12450 at 157.190 kb on - strand, within EX31_RS12450 at 157.203 kb on + strand, within EX31_RS12450 at 157.204 kb on - strand, within EX31_RS12450 at 157.204 kb on - strand, within EX31_RS12450 at 157.275 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.366 kb on - strand, within EX31_RS12450 at 157.366 kb on - strand, within EX31_RS12450 at 157.366 kb on - strand, within EX31_RS12450 at 157.507 kb on + strand, within EX31_RS12455 at 157.507 kb on + strand, within EX31_RS12455 at 157.507 kb on + strand, within EX31_RS12455 at 157.508 kb on - strand, within EX31_RS12455 at 157.508 kb on - strand, within EX31_RS12455 at 157.630 kb on + strand, within EX31_RS12455 at 157.659 kb on - strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.704 kb on - strand, within EX31_RS12455 at 157.704 kb on - strand, within EX31_RS12455 at 157.704 kb on - strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.712 kb on + strand, within EX31_RS12455 at 157.712 kb on + strand, within EX31_RS12455 at 157.713 kb on - strand, within EX31_RS12455 at 157.713 kb on - strand, within EX31_RS12455 at 157.713 kb on - strand, within EX31_RS12455 at 157.727 kb on + strand, within EX31_RS12455 at 157.727 kb on + strand, within EX31_RS12455 at 157.728 kb on - strand, within EX31_RS12455 at 157.761 kb on + strand at 157.761 kb on + strand at 157.761 kb on + strand at 157.761 kb on + strand at 157.761 kb on + strand at 157.762 kb on - strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.787 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.889 kb on + strand at 157.889 kb on + strand at 157.889 kb on + strand at 157.905 kb on + strand at 157.923 kb on + strand at 157.923 kb on + strand at 157.931 kb on + strand at 157.949 kb on + strand at 157.994 kb on + strand, within EX31_RS12460 at 157.994 kb on + strand, within EX31_RS12460 at 158.097 kb on + strand, within EX31_RS12460 at 158.097 kb on + strand, within EX31_RS12460 at 158.097 kb on + strand, within EX31_RS12460 at 158.129 kb on + strand, within EX31_RS12460 at 158.129 kb on + strand, within EX31_RS12460 at 158.129 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 156,975 + EX31_RS12450 0.25 -0.1 156,975 + EX31_RS12450 0.25 +0.3 156,975 + EX31_RS12450 0.25 +0.7 156,975 + EX31_RS12450 0.25 -0.1 156,975 + EX31_RS12450 0.25 -0.6 156,975 + EX31_RS12450 0.25 -1.6 156,976 - EX31_RS12450 0.25 -0.6 156,976 - EX31_RS12450 0.25 +0.4 156,979 + EX31_RS12450 0.25 +0.8 156,979 + EX31_RS12450 0.25 +0.6 156,979 + EX31_RS12450 0.25 +2.4 156,979 + EX31_RS12450 0.25 -0.6 156,980 - EX31_RS12450 0.26 +1.2 156,980 - EX31_RS12450 0.26 -0.7 156,980 - EX31_RS12450 0.26 -0.8 156,980 - EX31_RS12450 0.26 +0.7 157,015 + EX31_RS12450 0.31 +0.1 157,015 + EX31_RS12450 0.31 -1.4 157,016 - EX31_RS12450 0.31 -0.1 157,016 - EX31_RS12450 0.31 +0.6 157,031 - EX31_RS12450 0.34 -0.3 157,031 - EX31_RS12450 0.34 +1.0 157,049 + EX31_RS12450 0.36 -1.3 157,050 - EX31_RS12450 0.37 +1.1 157,058 - EX31_RS12450 0.38 -0.7 157,060 + EX31_RS12450 0.38 -0.2 157,060 + EX31_RS12450 0.38 +0.5 157,060 + EX31_RS12450 0.38 +1.1 157,061 - EX31_RS12450 0.38 -0.0 157,093 + EX31_RS12450 0.43 -0.7 157,093 + EX31_RS12450 0.43 -0.3 157,093 + EX31_RS12450 0.43 -1.0 157,094 - EX31_RS12450 0.44 -1.2 157,094 - EX31_RS12450 0.44 +0.5 157,129 + EX31_RS12450 0.49 -0.2 157,150 + EX31_RS12450 0.52 -0.1 157,150 + EX31_RS12450 0.52 -0.6 157,150 + EX31_RS12450 0.52 +0.5 157,150 + EX31_RS12450 0.52 -1.0 157,151 - EX31_RS12450 0.53 -0.1 157,151 - EX31_RS12450 0.53 -1.0 157,151 - EX31_RS12450 0.53 -1.1 157,151 - EX31_RS12450 0.53 -0.3 157,151 - EX31_RS12450 0.53 -0.1 157,151 - EX31_RS12450 0.53 -0.2 157,165 + EX31_RS12450 0.55 +1.4 157,166 - EX31_RS12450 0.55 -0.4 157,166 - EX31_RS12450 0.55 -2.6 157,166 - EX31_RS12450 0.55 +0.3 157,166 - EX31_RS12450 0.55 -0.4 157,190 - EX31_RS12450 0.59 -0.3 157,190 - EX31_RS12450 0.59 -0.6 157,203 + EX31_RS12450 0.61 +0.8 157,204 - EX31_RS12450 0.61 -0.4 157,204 - EX31_RS12450 0.61 -0.4 157,275 + EX31_RS12450 0.72 +1.1 157,306 + EX31_RS12450 0.77 +0.9 157,306 + EX31_RS12450 0.77 -0.0 157,306 + EX31_RS12450 0.77 +1.2 157,306 + EX31_RS12450 0.77 -0.3 157,306 + EX31_RS12450 0.77 -0.0 157,306 + EX31_RS12450 0.77 -0.0 157,307 - EX31_RS12450 0.77 +2.8 157,307 - EX31_RS12450 0.77 -0.3 157,307 - EX31_RS12450 0.77 +1.1 157,307 - EX31_RS12450 0.77 -1.8 157,307 - EX31_RS12450 0.77 -1.3 157,307 - EX31_RS12450 0.77 +0.3 157,365 + EX31_RS12450 0.86 +0.2 157,365 + EX31_RS12450 0.86 -0.2 157,365 + EX31_RS12450 0.86 +0.0 157,365 + EX31_RS12450 0.86 +0.0 157,366 - EX31_RS12450 0.86 +0.1 157,366 - EX31_RS12450 0.86 -0.1 157,366 - EX31_RS12450 0.86 +1.0 157,507 + EX31_RS12455 0.18 +0.1 157,507 + EX31_RS12455 0.18 -1.2 157,507 + EX31_RS12455 0.18 +0.1 157,508 - EX31_RS12455 0.19 -0.2 157,508 - EX31_RS12455 0.19 +0.0 157,630 + EX31_RS12455 0.57 -0.5 157,659 - EX31_RS12455 0.67 -0.8 157,703 + EX31_RS12455 0.81 +0.0 157,703 + EX31_RS12455 0.81 -1.3 157,703 + EX31_RS12455 0.81 -1.2 157,703 + EX31_RS12455 0.81 -0.1 157,704 - EX31_RS12455 0.81 +0.6 157,704 - EX31_RS12455 0.81 +2.0 157,704 - EX31_RS12455 0.81 +1.3 157,710 + EX31_RS12455 0.83 +0.4 157,710 + EX31_RS12455 0.83 -0.3 157,710 + EX31_RS12455 0.83 -0.3 157,710 + EX31_RS12455 0.83 -0.2 157,710 + EX31_RS12455 0.83 +0.1 157,710 + EX31_RS12455 0.83 -0.1 157,710 + EX31_RS12455 0.83 +0.2 157,710 + EX31_RS12455 0.83 -0.1 157,710 + EX31_RS12455 0.83 -0.1 157,710 + EX31_RS12455 0.83 -0.3 157,710 + EX31_RS12455 0.83 -0.3 157,710 + EX31_RS12455 0.83 -0.7 157,710 + EX31_RS12455 0.83 +0.9 157,710 + EX31_RS12455 0.83 +0.6 157,710 + EX31_RS12455 0.83 -0.7 157,711 - EX31_RS12455 0.83 -1.5 157,711 - EX31_RS12455 0.83 +1.4 157,711 - EX31_RS12455 0.83 -1.2 157,711 - EX31_RS12455 0.83 -0.2 157,711 - EX31_RS12455 0.83 -1.2 157,711 - EX31_RS12455 0.83 -1.5 157,711 - EX31_RS12455 0.83 -0.3 157,711 - EX31_RS12455 0.83 -0.3 157,712 + EX31_RS12455 0.83 -0.4 157,712 + EX31_RS12455 0.83 -0.3 157,713 - EX31_RS12455 0.84 -0.7 157,713 - EX31_RS12455 0.84 -0.1 157,713 - EX31_RS12455 0.84 +0.6 157,727 + EX31_RS12455 0.88 -0.4 157,727 + EX31_RS12455 0.88 -0.5 157,728 - EX31_RS12455 0.89 -0.2 157,761 + -0.5 157,761 + -0.4 157,761 + -0.8 157,761 + +0.4 157,761 + -0.4 157,762 - +0.0 157,765 + -0.6 157,765 + +0.6 157,765 + -1.8 157,765 + +0.3 157,765 + -0.8 157,765 + +1.2 157,765 + +0.1 157,766 - +0.5 157,766 - +0.6 157,766 - -0.5 157,766 - +1.1 157,766 - -0.4 157,766 - +0.6 157,767 + +0.1 157,767 + +0.0 157,767 + -0.4 157,767 + -0.7 157,767 + +1.0 157,767 + +0.9 157,767 + -1.1 157,767 + -0.6 157,768 - +0.7 157,768 - +0.7 157,768 - +0.5 157,768 - +0.7 157,768 - -0.4 157,768 - +0.8 157,787 + +0.9 157,805 + +1.5 157,805 + -0.3 157,805 + +0.2 157,805 + -0.4 157,805 + -1.6 157,805 + +0.7 157,805 + +0.3 157,805 + +1.0 157,805 + -2.0 157,805 + +0.6 157,806 - +0.9 157,806 - +0.5 157,806 - -0.2 157,806 - -0.0 157,806 - +0.9 157,806 - +0.4 157,806 - -0.4 157,889 + -0.6 157,889 + -1.3 157,889 + +0.7 157,905 + +0.0 157,923 + -1.9 157,923 + +0.0 157,931 + +0.2 157,949 + +1.0 157,994 + EX31_RS12460 0.24 -1.7 157,994 + EX31_RS12460 0.24 -1.4 158,097 + EX31_RS12460 0.65 -0.1 158,097 + EX31_RS12460 0.65 +0.2 158,097 + EX31_RS12460 0.65 -1.4 158,129 + EX31_RS12460 0.77 -0.8 158,129 + EX31_RS12460 0.77 +1.1 158,129 + EX31_RS12460 0.77 -1.0 158,139 + EX31_RS12460 0.81 +0.1 158,139 + EX31_RS12460 0.81 -1.9 158,139 + EX31_RS12460 0.81 +0.3 158,139 + EX31_RS12460 0.81 -2.4 158,139 + EX31_RS12460 0.81 -0.2 158,139 + EX31_RS12460 0.81 +0.9 158,139 + EX31_RS12460 0.81 -0.2 158,139 + EX31_RS12460 0.81 +0.8 158,139 + EX31_RS12460 0.81 -0.2 158,139 + EX31_RS12460 0.81 +0.6 158,139 + EX31_RS12460 0.81 -0.4 158,182 + -0.6
Or see this region's nucleotide sequence