Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11300 and EX31_RS11305 are separated by 77 nucleotides EX31_RS11305 and EX31_RS11310 are separated by 11 nucleotides EX31_RS11310 and EX31_RS11315 are separated by 6 nucleotides EX31_RS11315 and EX31_RS11320 are separated by 113 nucleotides
EX31_RS11300: EX31_RS11300 - flagellar hook protein FlgE, at 192,685 to 193,929
_RS11300
EX31_RS11305: EX31_RS11305 - flagellar hook assembly protein FlgD, at 194,007 to 194,711
_RS11305
EX31_RS11310: EX31_RS11310 - flagellar basal body rod protein FlgC, at 194,723 to 195,127
_RS11310
EX31_RS11315: EX31_RS11315 - flagellar basal body rod protein FlgB, at 195,134 to 195,544
_RS11315
EX31_RS11320: EX31_RS11320 - flagellar basal body P-ring formation protein FlgA, at 195,658 to 196,440
_RS11320
Position (kb)
194
195
196 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 193.943 kb on + strand at 193.943 kb on + strand at 193.944 kb on - strand at 193.944 kb on - strand at 193.944 kb on - strand at 193.944 kb on - strand at 193.944 kb on - strand at 194.091 kb on + strand, within EX31_RS11305 at 194.092 kb on - strand, within EX31_RS11305 at 194.092 kb on - strand, within EX31_RS11305 at 194.092 kb on - strand, within EX31_RS11305 at 194.092 kb on - strand, within EX31_RS11305 at 194.110 kb on + strand, within EX31_RS11305 at 194.110 kb on + strand, within EX31_RS11305 at 194.111 kb on - strand, within EX31_RS11305 at 194.111 kb on - strand, within EX31_RS11305 at 194.111 kb on - strand, within EX31_RS11305 at 194.111 kb on - strand, within EX31_RS11305 at 194.171 kb on - strand, within EX31_RS11305 at 194.171 kb on - strand, within EX31_RS11305 at 194.208 kb on - strand, within EX31_RS11305 at 194.219 kb on + strand, within EX31_RS11305 at 194.220 kb on - strand, within EX31_RS11305 at 194.220 kb on - strand, within EX31_RS11305 at 194.220 kb on - strand, within EX31_RS11305 at 194.220 kb on - strand, within EX31_RS11305 at 194.221 kb on + strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.222 kb on - strand, within EX31_RS11305 at 194.478 kb on - strand, within EX31_RS11305 at 194.608 kb on - strand, within EX31_RS11305 at 194.702 kb on + strand at 194.702 kb on + strand at 194.794 kb on - strand, within EX31_RS11310 at 194.794 kb on - strand, within EX31_RS11310 at 194.840 kb on + strand, within EX31_RS11310 at 194.840 kb on + strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.841 kb on - strand, within EX31_RS11310 at 194.859 kb on + strand, within EX31_RS11310 at 194.860 kb on - strand, within EX31_RS11310 at 194.860 kb on - strand, within EX31_RS11310 at 194.860 kb on - strand, within EX31_RS11310 at 194.860 kb on - strand, within EX31_RS11310 at 194.895 kb on + strand, within EX31_RS11310 at 194.895 kb on + strand, within EX31_RS11310 at 194.896 kb on - strand, within EX31_RS11310 at 195.002 kb on - strand, within EX31_RS11310 at 195.011 kb on - strand, within EX31_RS11310 at 195.050 kb on - strand, within EX31_RS11310 at 195.113 kb on - strand at 195.132 kb on + strand at 195.133 kb on - strand at 195.133 kb on - strand at 195.136 kb on - strand at 195.311 kb on + strand, within EX31_RS11315 at 195.311 kb on + strand, within EX31_RS11315 at 195.312 kb on - strand, within EX31_RS11315 at 195.312 kb on - strand, within EX31_RS11315 at 195.312 kb on - strand, within EX31_RS11315 at 195.312 kb on - strand, within EX31_RS11315 at 195.432 kb on + strand, within EX31_RS11315 at 195.519 kb on - strand at 195.519 kb on - strand at 195.582 kb on + strand at 195.582 kb on + strand at 195.582 kb on + strand at 195.582 kb on + strand at 195.583 kb on - strand at 195.583 kb on - strand at 195.615 kb on - strand at 195.615 kb on - strand at 195.615 kb on - strand at 195.634 kb on - strand at 195.645 kb on + strand at 195.645 kb on + strand at 195.666 kb on - strand at 195.680 kb on + strand at 195.680 kb on + strand at 195.680 kb on + strand at 195.680 kb on + strand at 195.681 kb on - strand at 195.683 kb on + strand at 195.683 kb on + strand at 195.683 kb on + strand at 195.684 kb on - strand at 195.698 kb on + strand at 195.699 kb on + strand at 195.700 kb on - strand at 195.726 kb on - strand at 195.732 kb on + strand at 195.732 kb on + strand at 195.787 kb on + strand, within EX31_RS11320 at 195.787 kb on + strand, within EX31_RS11320 at 195.788 kb on - strand, within EX31_RS11320 at 195.837 kb on + strand, within EX31_RS11320 at 195.843 kb on + strand, within EX31_RS11320 at 195.887 kb on - strand, within EX31_RS11320 at 195.887 kb on - strand, within EX31_RS11320 at 195.953 kb on + strand, within EX31_RS11320 at 195.954 kb on - strand, within EX31_RS11320 at 195.954 kb on - strand, within EX31_RS11320 at 195.967 kb on - strand, within EX31_RS11320 at 196.020 kb on + strand, within EX31_RS11320 at 196.040 kb on + strand, within EX31_RS11320 at 196.040 kb on + strand, within EX31_RS11320 at 196.040 kb on + strand, within EX31_RS11320 at 196.041 kb on - strand, within EX31_RS11320 at 196.069 kb on + strand, within EX31_RS11320
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 193,943 + -1.0 193,943 + +0.2 193,944 - -0.5 193,944 - -1.7 193,944 - +2.0 193,944 - -1.3 193,944 - +0.2 194,091 + EX31_RS11305 0.12 -1.5 194,092 - EX31_RS11305 0.12 -0.8 194,092 - EX31_RS11305 0.12 -0.2 194,092 - EX31_RS11305 0.12 +1.3 194,092 - EX31_RS11305 0.12 -1.2 194,110 + EX31_RS11305 0.15 -0.1 194,110 + EX31_RS11305 0.15 -0.8 194,111 - EX31_RS11305 0.15 -0.4 194,111 - EX31_RS11305 0.15 -0.1 194,111 - EX31_RS11305 0.15 -1.2 194,111 - EX31_RS11305 0.15 -1.6 194,171 - EX31_RS11305 0.23 -0.5 194,171 - EX31_RS11305 0.23 -2.0 194,208 - EX31_RS11305 0.29 +0.7 194,219 + EX31_RS11305 0.30 +0.2 194,220 - EX31_RS11305 0.30 -0.1 194,220 - EX31_RS11305 0.30 -1.4 194,220 - EX31_RS11305 0.30 +0.0 194,220 - EX31_RS11305 0.30 +0.8 194,221 + EX31_RS11305 0.30 +0.5 194,222 - EX31_RS11305 0.30 -0.6 194,222 - EX31_RS11305 0.30 -0.4 194,222 - EX31_RS11305 0.30 +0.8 194,222 - EX31_RS11305 0.30 -1.9 194,222 - EX31_RS11305 0.30 +1.6 194,222 - EX31_RS11305 0.30 -0.4 194,222 - EX31_RS11305 0.30 -1.9 194,222 - EX31_RS11305 0.30 +0.0 194,478 - EX31_RS11305 0.67 -2.7 194,608 - EX31_RS11305 0.85 -0.3 194,702 + +2.6 194,702 + -1.8 194,794 - EX31_RS11310 0.18 -0.5 194,794 - EX31_RS11310 0.18 +1.0 194,840 + EX31_RS11310 0.29 +0.8 194,840 + EX31_RS11310 0.29 -1.5 194,841 - EX31_RS11310 0.29 -1.4 194,841 - EX31_RS11310 0.29 +0.0 194,841 - EX31_RS11310 0.29 -2.0 194,841 - EX31_RS11310 0.29 -1.0 194,841 - EX31_RS11310 0.29 -0.2 194,841 - EX31_RS11310 0.29 -2.3 194,841 - EX31_RS11310 0.29 -0.3 194,859 + EX31_RS11310 0.34 -0.5 194,860 - EX31_RS11310 0.34 -0.8 194,860 - EX31_RS11310 0.34 -0.8 194,860 - EX31_RS11310 0.34 -0.5 194,860 - EX31_RS11310 0.34 +1.5 194,895 + EX31_RS11310 0.42 -0.8 194,895 + EX31_RS11310 0.42 -0.8 194,896 - EX31_RS11310 0.43 -0.3 195,002 - EX31_RS11310 0.69 -1.4 195,011 - EX31_RS11310 0.71 -1.3 195,050 - EX31_RS11310 0.81 +0.1 195,113 - -1.5 195,132 + -1.1 195,133 - +0.3 195,133 - -0.8 195,136 - -1.4 195,311 + EX31_RS11315 0.43 +0.5 195,311 + EX31_RS11315 0.43 -0.5 195,312 - EX31_RS11315 0.43 -0.0 195,312 - EX31_RS11315 0.43 +1.6 195,312 - EX31_RS11315 0.43 -1.4 195,312 - EX31_RS11315 0.43 +1.2 195,432 + EX31_RS11315 0.73 -2.5 195,519 - -0.6 195,519 - -2.0 195,582 + +0.8 195,582 + -2.3 195,582 + +2.0 195,582 + -0.4 195,583 - -0.8 195,583 - -1.3 195,615 - -1.0 195,615 - -1.8 195,615 - +2.0 195,634 - +1.6 195,645 + +0.7 195,645 + +0.8 195,666 - +0.8 195,680 + +0.8 195,680 + +0.0 195,680 + -1.5 195,680 + +0.7 195,681 - -0.8 195,683 + -0.1 195,683 + -0.7 195,683 + +0.1 195,684 - -0.1 195,698 + +0.2 195,699 + -1.0 195,700 - +1.6 195,726 - +0.1 195,732 + -0.1 195,732 + -0.0 195,787 + EX31_RS11320 0.16 +0.1 195,787 + EX31_RS11320 0.16 +0.3 195,788 - EX31_RS11320 0.17 -0.4 195,837 + EX31_RS11320 0.23 +0.6 195,843 + EX31_RS11320 0.24 -0.7 195,887 - EX31_RS11320 0.29 +1.5 195,887 - EX31_RS11320 0.29 -0.1 195,953 + EX31_RS11320 0.38 +1.0 195,954 - EX31_RS11320 0.38 +2.0 195,954 - EX31_RS11320 0.38 +0.3 195,967 - EX31_RS11320 0.39 +1.5 196,020 + EX31_RS11320 0.46 -0.8 196,040 + EX31_RS11320 0.49 +0.7 196,040 + EX31_RS11320 0.49 -1.0 196,040 + EX31_RS11320 0.49 +0.0 196,041 - EX31_RS11320 0.49 -0.5 196,069 + EX31_RS11320 0.52 -2.7
Or see this region's nucleotide sequence