Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10510 and EX31_RS10515 are separated by 181 nucleotides EX31_RS10515 and EX31_RS10520 are separated by 65 nucleotides EX31_RS10520 and EX31_RS10525 are separated by 236 nucleotides
EX31_RS10510: EX31_RS10510 - sodium/proton antiporter NhaB, at 16,381 to 17,958
_RS10510
EX31_RS10515: EX31_RS10515 - disulfide bond formation protein DsbB, at 18,140 to 18,670
_RS10515
EX31_RS10520: EX31_RS10520 - hypothetical protein, at 18,736 to 18,933
_RS10520
EX31_RS10525: EX31_RS10525 - AI-2E family transporter, at 19,170 to 20,279
_RS10525
Position (kb) 18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 17.830 kb on + strand at 17.830 kb on + strand at 17.831 kb on - strand at 17.899 kb on + strand at 17.900 kb on - strand at 17.989 kb on + strand at 18.012 kb on - strand at 18.040 kb on + strand at 18.041 kb on - strand at 18.042 kb on + strand at 18.049 kb on + strand at 18.099 kb on + strand at 18.114 kb on + strand at 18.114 kb on + strand at 18.118 kb on + strand at 18.139 kb on + strand at 18.150 kb on - strand at 18.230 kb on + strand, within EX31_RS10515 at 18.231 kb on - strand, within EX31_RS10515 at 18.249 kb on + strand, within EX31_RS10515 at 18.253 kb on + strand, within EX31_RS10515 at 18.253 kb on + strand, within EX31_RS10515 at 18.253 kb on + strand, within EX31_RS10515 at 18.254 kb on - strand, within EX31_RS10515 at 18.271 kb on + strand, within EX31_RS10515 at 18.271 kb on + strand, within EX31_RS10515 at 18.271 kb on + strand, within EX31_RS10515 at 18.271 kb on + strand, within EX31_RS10515 at 18.271 kb on + strand, within EX31_RS10515 at 18.275 kb on + strand, within EX31_RS10515 at 18.276 kb on - strand, within EX31_RS10515 at 18.374 kb on + strand, within EX31_RS10515 at 18.760 kb on + strand, within EX31_RS10520 at 18.760 kb on + strand, within EX31_RS10520 at 18.760 kb on + strand, within EX31_RS10520 at 18.760 kb on + strand, within EX31_RS10520 at 18.760 kb on + strand, within EX31_RS10520 at 18.760 kb on + strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.761 kb on - strand, within EX31_RS10520 at 18.763 kb on + strand, within EX31_RS10520 at 18.764 kb on - strand, within EX31_RS10520 at 18.809 kb on + strand, within EX31_RS10520 at 18.812 kb on + strand, within EX31_RS10520 at 18.914 kb on + strand at 18.916 kb on + strand at 18.917 kb on - strand at 19.105 kb on - strand at 19.105 kb on - strand at 19.132 kb on - strand at 19.132 kb on - strand at 19.136 kb on + strand at 19.137 kb on - strand at 19.137 kb on - strand at 19.137 kb on - strand at 19.223 kb on - strand at 19.243 kb on + strand at 19.243 kb on + strand at 19.244 kb on - strand at 19.244 kb on - strand at 19.244 kb on - strand at 19.244 kb on - strand at 19.244 kb on - strand at 19.257 kb on + strand at 19.258 kb on - strand at 19.268 kb on - strand at 19.281 kb on - strand, within EX31_RS10525 at 19.408 kb on + strand, within EX31_RS10525 at 19.408 kb on + strand, within EX31_RS10525 at 19.409 kb on - strand, within EX31_RS10525 at 19.599 kb on - strand, within EX31_RS10525 at 19.599 kb on - strand, within EX31_RS10525 at 19.605 kb on + strand, within EX31_RS10525 at 19.630 kb on + strand, within EX31_RS10525 at 19.630 kb on + strand, within EX31_RS10525 at 19.631 kb on - strand, within EX31_RS10525 at 19.631 kb on - strand, within EX31_RS10525 at 19.634 kb on + strand, within EX31_RS10525 at 19.634 kb on + strand, within EX31_RS10525 at 19.635 kb on - strand, within EX31_RS10525 at 19.635 kb on - strand, within EX31_RS10525 at 19.635 kb on - strand, within EX31_RS10525 at 19.635 kb on - strand, within EX31_RS10525 at 19.677 kb on + strand, within EX31_RS10525 at 19.677 kb on + strand, within EX31_RS10525 at 19.677 kb on + strand, within EX31_RS10525 at 19.677 kb on + strand, within EX31_RS10525 at 19.678 kb on - strand, within EX31_RS10525 at 19.678 kb on - strand, within EX31_RS10525 at 19.678 kb on - strand, within EX31_RS10525 at 19.752 kb on + strand, within EX31_RS10525 at 19.756 kb on + strand, within EX31_RS10525 at 19.756 kb on + strand, within EX31_RS10525 at 19.757 kb on - strand, within EX31_RS10525 at 19.757 kb on - strand, within EX31_RS10525 at 19.772 kb on + strand, within EX31_RS10525 at 19.804 kb on + strand, within EX31_RS10525 at 19.804 kb on + strand, within EX31_RS10525 at 19.831 kb on + strand, within EX31_RS10525 at 19.860 kb on + strand, within EX31_RS10525
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 17,830 + +0.0 17,830 + -0.4 17,831 - +1.5 17,899 + +2.0 17,900 - +0.8 17,989 + -1.3 18,012 - +0.2 18,040 + -2.8 18,041 - -0.3 18,042 + -1.8 18,049 + +0.5 18,099 + +2.0 18,114 + -0.1 18,114 + +0.3 18,118 + +2.5 18,139 + +0.8 18,150 - +1.5 18,230 + EX31_RS10515 0.17 -0.7 18,231 - EX31_RS10515 0.17 +2.0 18,249 + EX31_RS10515 0.21 -1.1 18,253 + EX31_RS10515 0.21 -0.8 18,253 + EX31_RS10515 0.21 -1.3 18,253 + EX31_RS10515 0.21 -0.5 18,254 - EX31_RS10515 0.21 -1.5 18,271 + EX31_RS10515 0.25 -0.4 18,271 + EX31_RS10515 0.25 +1.0 18,271 + EX31_RS10515 0.25 +1.5 18,271 + EX31_RS10515 0.25 -0.9 18,271 + EX31_RS10515 0.25 -0.1 18,275 + EX31_RS10515 0.25 +1.5 18,276 - EX31_RS10515 0.26 -1.9 18,374 + EX31_RS10515 0.44 -0.6 18,760 + EX31_RS10520 0.12 +2.5 18,760 + EX31_RS10520 0.12 -1.5 18,760 + EX31_RS10520 0.12 -0.1 18,760 + EX31_RS10520 0.12 +0.3 18,760 + EX31_RS10520 0.12 +0.9 18,760 + EX31_RS10520 0.12 -1.0 18,761 - EX31_RS10520 0.13 +1.8 18,761 - EX31_RS10520 0.13 +1.0 18,761 - EX31_RS10520 0.13 +0.5 18,761 - EX31_RS10520 0.13 +2.0 18,761 - EX31_RS10520 0.13 +2.0 18,761 - EX31_RS10520 0.13 +1.4 18,761 - EX31_RS10520 0.13 +0.5 18,763 + EX31_RS10520 0.14 +0.6 18,764 - EX31_RS10520 0.14 +2.8 18,809 + EX31_RS10520 0.37 +1.0 18,812 + EX31_RS10520 0.38 +0.2 18,914 + +0.5 18,916 + -1.0 18,917 - +1.5 19,105 - +1.5 19,105 - +1.2 19,132 - -1.0 19,132 - +0.0 19,136 + -0.4 19,137 - +0.0 19,137 - +0.2 19,137 - -0.2 19,223 - -0.0 19,243 + +0.6 19,243 + -0.5 19,244 - -1.0 19,244 - -2.7 19,244 - -0.1 19,244 - +1.3 19,244 - +0.9 19,257 + +0.6 19,258 - -1.4 19,268 - -1.8 19,281 - EX31_RS10525 0.10 -0.8 19,408 + EX31_RS10525 0.21 +0.3 19,408 + EX31_RS10525 0.21 +1.0 19,409 - EX31_RS10525 0.22 +1.2 19,599 - EX31_RS10525 0.39 -1.1 19,599 - EX31_RS10525 0.39 +0.0 19,605 + EX31_RS10525 0.39 +1.0 19,630 + EX31_RS10525 0.41 -0.0 19,630 + EX31_RS10525 0.41 +1.4 19,631 - EX31_RS10525 0.42 -1.1 19,631 - EX31_RS10525 0.42 +1.2 19,634 + EX31_RS10525 0.42 -0.5 19,634 + EX31_RS10525 0.42 +0.8 19,635 - EX31_RS10525 0.42 +0.4 19,635 - EX31_RS10525 0.42 +1.0 19,635 - EX31_RS10525 0.42 +0.2 19,635 - EX31_RS10525 0.42 -0.4 19,677 + EX31_RS10525 0.46 +0.5 19,677 + EX31_RS10525 0.46 -1.0 19,677 + EX31_RS10525 0.46 +1.0 19,677 + EX31_RS10525 0.46 -0.2 19,678 - EX31_RS10525 0.46 +1.5 19,678 - EX31_RS10525 0.46 +0.5 19,678 - EX31_RS10525 0.46 -0.5 19,752 + EX31_RS10525 0.52 -0.2 19,756 + EX31_RS10525 0.53 +0.5 19,756 + EX31_RS10525 0.53 -0.3 19,757 - EX31_RS10525 0.53 +0.1 19,757 - EX31_RS10525 0.53 +1.0 19,772 + EX31_RS10525 0.54 -0.5 19,804 + EX31_RS10525 0.57 -0.7 19,804 + EX31_RS10525 0.57 +1.0 19,831 + EX31_RS10525 0.60 -0.5 19,860 + EX31_RS10525 0.62 -0.3
Or see this region's nucleotide sequence