Strain Fitness in Rahnella sp. WP5 around EX31_RS09375

Experiment: Ammonium chloride (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS09370 and EX31_RS09375 are separated by 285 nucleotidesEX31_RS09375 and EX31_RS09380 are separated by 350 nucleotides EX31_RS09370: EX31_RS09370 - ABC transporter substrate-binding protein, at 77,342 to 78,910 _RS09370 EX31_RS09375: EX31_RS09375 - gamma-glutamylcyclotransferase, at 79,196 to 79,885 _RS09375 EX31_RS09380: EX31_RS09380 - putative cation transport regulator ChaB, at 80,236 to 80,469 _RS09380 Position (kb) 79 80Strain fitness (log2 ratio) -1 0 1 2at 78.285 kb on + strand, within EX31_RS09370at 78.286 kb on - strand, within EX31_RS09370at 78.286 kb on - strand, within EX31_RS09370at 78.391 kb on + strand, within EX31_RS09370at 78.399 kb on - strand, within EX31_RS09370at 78.655 kb on + strand, within EX31_RS09370at 78.656 kb on - strand, within EX31_RS09370at 78.742 kb on + strand, within EX31_RS09370at 78.746 kb on - strand, within EX31_RS09370at 78.990 kb on + strandat 78.994 kb on + strandat 78.995 kb on - strandat 79.039 kb on + strandat 79.074 kb on - strandat 79.129 kb on + strandat 79.145 kb on + strandat 79.198 kb on - strandat 79.198 kb on - strandat 79.247 kb on + strandat 79.248 kb on - strandat 79.291 kb on - strand, within EX31_RS09375at 79.324 kb on - strand, within EX31_RS09375at 79.401 kb on + strand, within EX31_RS09375at 79.647 kb on - strand, within EX31_RS09375at 79.647 kb on - strand, within EX31_RS09375at 79.666 kb on - strand, within EX31_RS09375at 79.815 kb on - strand, within EX31_RS09375at 79.881 kb on - strandat 79.881 kb on - strandat 79.902 kb on - strandat 79.909 kb on - strandat 79.909 kb on - strandat 79.965 kb on + strandat 79.966 kb on - strandat 79.966 kb on - strandat 80.018 kb on - strandat 80.018 kb on - strandat 80.033 kb on - strandat 80.035 kb on - strandat 80.035 kb on - strandat 80.055 kb on + strandat 80.144 kb on - strandat 80.147 kb on + strandat 80.235 kb on - strandat 80.248 kb on - strandat 80.248 kb on - strandat 80.277 kb on + strand, within EX31_RS09380at 80.278 kb on - strand, within EX31_RS09380at 80.310 kb on + strand, within EX31_RS09380at 80.311 kb on - strand, within EX31_RS09380at 80.311 kb on - strand, within EX31_RS09380at 80.352 kb on + strand, within EX31_RS09380at 80.364 kb on - strand, within EX31_RS09380at 80.394 kb on + strand, within EX31_RS09380at 80.395 kb on - strand, within EX31_RS09380at 80.396 kb on + strand, within EX31_RS09380at 80.396 kb on + strand, within EX31_RS09380at 80.396 kb on + strand, within EX31_RS09380at 80.397 kb on - strand, within EX31_RS09380at 80.397 kb on - strand, within EX31_RS09380at 80.397 kb on - strand, within EX31_RS09380at 80.397 kb on - strand, within EX31_RS09380at 80.397 kb on - strand, within EX31_RS09380at 80.544 kb on - strandat 80.551 kb on - strandat 80.782 kb on - strandat 80.843 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking
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78,285 + EX31_RS09370 0.60 +1.9
78,286 - EX31_RS09370 0.60 -0.9
78,286 - EX31_RS09370 0.60 -0.5
78,391 + EX31_RS09370 0.67 -0.6
78,399 - EX31_RS09370 0.67 +0.5
78,655 + EX31_RS09370 0.84 +1.4
78,656 - EX31_RS09370 0.84 +0.9
78,742 + EX31_RS09370 0.89 +0.0
78,746 - EX31_RS09370 0.89 -1.3
78,990 + +1.1
78,994 + -0.6
78,995 - -0.6
79,039 + -0.5
79,074 - +1.3
79,129 + +1.0
79,145 + -0.9
79,198 - -0.6
79,198 - +0.1
79,247 + -0.2
79,248 - -1.7
79,291 - EX31_RS09375 0.14 +0.4
79,324 - EX31_RS09375 0.19 +1.1
79,401 + EX31_RS09375 0.30 +0.1
79,647 - EX31_RS09375 0.65 +1.0
79,647 - EX31_RS09375 0.65 +0.6
79,666 - EX31_RS09375 0.68 +0.1
79,815 - EX31_RS09375 0.90 +2.2
79,881 - +0.1
79,881 - +1.0
79,902 - +2.2
79,909 - -0.6
79,909 - -1.1
79,965 + -0.6
79,966 - -0.3
79,966 - +0.3
80,018 - +1.3
80,018 - -0.0
80,033 - -0.3
80,035 - -1.2
80,035 - -0.3
80,055 + +2.3
80,144 - -0.5
80,147 + -1.3
80,235 - +0.3
80,248 - -0.6
80,248 - +0.2
80,277 + EX31_RS09380 0.18 +0.3
80,278 - EX31_RS09380 0.18 +0.2
80,310 + EX31_RS09380 0.32 -0.1
80,311 - EX31_RS09380 0.32 +0.3
80,311 - EX31_RS09380 0.32 +0.8
80,352 + EX31_RS09380 0.50 +0.9
80,364 - EX31_RS09380 0.55 +0.9
80,394 + EX31_RS09380 0.68 -0.5
80,395 - EX31_RS09380 0.68 -0.4
80,396 + EX31_RS09380 0.68 +0.1
80,396 + EX31_RS09380 0.68 -1.2
80,396 + EX31_RS09380 0.68 +1.9
80,397 - EX31_RS09380 0.69 -0.4
80,397 - EX31_RS09380 0.69 +0.5
80,397 - EX31_RS09380 0.69 +0.1
80,397 - EX31_RS09380 0.69 -0.9
80,397 - EX31_RS09380 0.69 +0.4
80,544 - -0.7
80,551 - -0.5
80,782 - -0.2
80,843 - -0.5

Or see this region's nucleotide sequence