Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08195 and EX31_RS08200 are separated by 61 nucleotides EX31_RS08200 and EX31_RS08205 are separated by 33 nucleotides EX31_RS08205 and EX31_RS08210 are separated by 80 nucleotides EX31_RS08210 and EX31_RS08215 are separated by 278 nucleotides
EX31_RS08195: EX31_RS08195 - RNA polymerase sigma factor RpoD, at 132,951 to 134,789
_RS08195
EX31_RS08200: EX31_RS08200 - hypothetical protein, at 134,851 to 135,318
_RS08200
EX31_RS08205: EX31_RS08205 - hypothetical protein, at 135,352 to 135,954
_RS08205
EX31_RS08210: EX31_RS08210 - hypothetical protein, at 136,035 to 136,526
_RS08210
EX31_RS08215: EX31_RS08215 - G/U mismatch-specific DNA glycosylase, at 136,805 to 137,308
_RS08215
Position (kb)
135
136 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 134.850 kb on - strand at 134.887 kb on - strand at 134.936 kb on - strand, within EX31_RS08200 at 134.936 kb on - strand, within EX31_RS08200 at 135.050 kb on + strand, within EX31_RS08200 at 135.080 kb on - strand, within EX31_RS08200 at 135.105 kb on - strand, within EX31_RS08200 at 135.145 kb on - strand, within EX31_RS08200 at 135.171 kb on + strand, within EX31_RS08200 at 135.172 kb on - strand, within EX31_RS08200 at 135.322 kb on - strand at 135.336 kb on - strand at 135.401 kb on + strand at 135.401 kb on + strand at 135.401 kb on + strand at 135.402 kb on - strand at 135.406 kb on - strand at 135.406 kb on - strand at 135.471 kb on + strand, within EX31_RS08205 at 135.473 kb on + strand, within EX31_RS08205 at 135.474 kb on - strand, within EX31_RS08205 at 135.474 kb on - strand, within EX31_RS08205 at 135.474 kb on - strand, within EX31_RS08205 at 135.495 kb on - strand, within EX31_RS08205 at 135.567 kb on + strand, within EX31_RS08205 at 135.567 kb on + strand, within EX31_RS08205 at 135.568 kb on - strand, within EX31_RS08205 at 135.599 kb on - strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.620 kb on + strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.621 kb on - strand, within EX31_RS08205 at 135.628 kb on - strand, within EX31_RS08205 at 135.628 kb on - strand, within EX31_RS08205 at 135.628 kb on - strand, within EX31_RS08205 at 135.681 kb on - strand, within EX31_RS08205 at 135.699 kb on + strand, within EX31_RS08205 at 135.700 kb on - strand, within EX31_RS08205 at 135.700 kb on - strand, within EX31_RS08205 at 135.700 kb on - strand, within EX31_RS08205 at 135.789 kb on - strand, within EX31_RS08205 at 135.958 kb on - strand at 135.969 kb on - strand at 136.157 kb on + strand, within EX31_RS08210 at 136.232 kb on - strand, within EX31_RS08210 at 136.268 kb on - strand, within EX31_RS08210 at 136.294 kb on + strand, within EX31_RS08210 at 136.295 kb on - strand, within EX31_RS08210 at 136.323 kb on - strand, within EX31_RS08210 at 136.323 kb on - strand, within EX31_RS08210 at 136.323 kb on - strand, within EX31_RS08210 at 136.520 kb on + strand at 136.521 kb on - strand at 136.526 kb on + strand at 136.560 kb on + strand at 136.601 kb on + strand at 136.602 kb on - strand at 136.642 kb on + strand at 136.648 kb on - strand at 136.651 kb on + strand at 136.652 kb on - strand at 136.661 kb on + strand at 136.661 kb on + strand at 136.662 kb on - strand at 136.685 kb on + strand at 136.686 kb on - strand at 136.686 kb on - strand at 136.697 kb on - strand at 136.739 kb on + strand at 136.740 kb on - strand at 136.750 kb on + strand at 136.751 kb on - strand at 136.787 kb on + strand at 136.788 kb on - strand at 136.820 kb on + strand at 136.826 kb on + strand at 136.832 kb on + strand at 136.833 kb on - strand at 136.850 kb on + strand at 136.951 kb on + strand, within EX31_RS08215 at 136.951 kb on + strand, within EX31_RS08215 at 136.952 kb on - strand, within EX31_RS08215
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 134,850 - +0.2 134,887 - -0.7 134,936 - EX31_RS08200 0.18 +1.3 134,936 - EX31_RS08200 0.18 +1.0 135,050 + EX31_RS08200 0.43 +0.3 135,080 - EX31_RS08200 0.49 +0.7 135,105 - EX31_RS08200 0.54 +0.6 135,145 - EX31_RS08200 0.63 +0.2 135,171 + EX31_RS08200 0.68 +1.7 135,172 - EX31_RS08200 0.69 +0.1 135,322 - -0.1 135,336 - -0.4 135,401 + -0.1 135,401 + +1.4 135,401 + -0.7 135,402 - -1.3 135,406 - +0.1 135,406 - +2.4 135,471 + EX31_RS08205 0.20 +1.4 135,473 + EX31_RS08205 0.20 -0.7 135,474 - EX31_RS08205 0.20 +1.1 135,474 - EX31_RS08205 0.20 +0.4 135,474 - EX31_RS08205 0.20 -1.6 135,495 - EX31_RS08205 0.24 +0.9 135,567 + EX31_RS08205 0.36 +0.6 135,567 + EX31_RS08205 0.36 +1.2 135,568 - EX31_RS08205 0.36 -0.1 135,599 - EX31_RS08205 0.41 +2.0 135,620 + EX31_RS08205 0.44 -0.8 135,620 + EX31_RS08205 0.44 +1.0 135,620 + EX31_RS08205 0.44 +1.5 135,620 + EX31_RS08205 0.44 +2.2 135,620 + EX31_RS08205 0.44 +0.1 135,620 + EX31_RS08205 0.44 -0.6 135,620 + EX31_RS08205 0.44 +1.6 135,621 - EX31_RS08205 0.45 -0.9 135,621 - EX31_RS08205 0.45 -0.1 135,621 - EX31_RS08205 0.45 +0.1 135,621 - EX31_RS08205 0.45 -0.6 135,621 - EX31_RS08205 0.45 -0.4 135,621 - EX31_RS08205 0.45 +0.1 135,621 - EX31_RS08205 0.45 -0.7 135,621 - EX31_RS08205 0.45 +0.9 135,621 - EX31_RS08205 0.45 +0.6 135,621 - EX31_RS08205 0.45 -0.0 135,621 - EX31_RS08205 0.45 +0.6 135,621 - EX31_RS08205 0.45 -0.9 135,621 - EX31_RS08205 0.45 +0.9 135,621 - EX31_RS08205 0.45 +0.3 135,621 - EX31_RS08205 0.45 -0.3 135,621 - EX31_RS08205 0.45 -0.6 135,621 - EX31_RS08205 0.45 +1.0 135,621 - EX31_RS08205 0.45 +0.8 135,628 - EX31_RS08205 0.46 -2.1 135,628 - EX31_RS08205 0.46 +0.4 135,628 - EX31_RS08205 0.46 +1.8 135,681 - EX31_RS08205 0.55 +0.0 135,699 + EX31_RS08205 0.58 -0.6 135,700 - EX31_RS08205 0.58 -0.7 135,700 - EX31_RS08205 0.58 -0.2 135,700 - EX31_RS08205 0.58 -1.1 135,789 - EX31_RS08205 0.72 +0.5 135,958 - +1.0 135,969 - +0.4 136,157 + EX31_RS08210 0.25 +0.4 136,232 - EX31_RS08210 0.40 -0.7 136,268 - EX31_RS08210 0.47 -1.2 136,294 + EX31_RS08210 0.53 +0.1 136,295 - EX31_RS08210 0.53 +0.3 136,323 - EX31_RS08210 0.59 -0.7 136,323 - EX31_RS08210 0.59 -0.3 136,323 - EX31_RS08210 0.59 +0.3 136,520 + +0.0 136,521 - +2.4 136,526 + +0.9 136,560 + -0.5 136,601 + -0.1 136,602 - +0.5 136,642 + -1.5 136,648 - -2.6 136,651 + -0.9 136,652 - -0.5 136,661 + -1.5 136,661 + +0.9 136,662 - +0.2 136,685 + +0.2 136,686 - -0.0 136,686 - +0.4 136,697 - -0.2 136,739 + -0.2 136,740 - -2.3 136,750 + +1.0 136,751 - +0.5 136,787 + +1.1 136,788 - -0.4 136,820 + -0.3 136,826 + +1.5 136,832 + -0.4 136,833 - -0.3 136,850 + +0.5 136,951 + EX31_RS08215 0.29 -1.3 136,951 + EX31_RS08215 0.29 +2.1 136,952 - EX31_RS08215 0.29 +1.5
Or see this region's nucleotide sequence