Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07535 and EX31_RS07540 are separated by 289 nucleotides EX31_RS07540 and EX31_RS07545 are separated by 99 nucleotides EX31_RS07545 and EX31_RS25970 are separated by 118 nucleotides EX31_RS25970 and EX31_RS25230 overlap by 1 nucleotides EX31_RS25230 and EX31_RS07550 are separated by 68 nucleotides
EX31_RS07535: EX31_RS07535 - cytochrome b/b6 domain-containing protein, at 322,827 to 323,399
_RS07535
EX31_RS07540: EX31_RS07540 - helix-turn-helix transcriptional regulator, at 323,689 to 324,066
_RS07540
EX31_RS07545: EX31_RS07545 - SDR family oxidoreductase, at 324,166 to 324,897
_RS07545
EX31_RS25970: EX31_RS25970 - glutathione transferase GstA, at 325,016 to 325,123
_RS25970
EX31_RS25230: EX31_RS25230 - LysR family transcriptional regulator, at 325,123 to 325,377
_RS25230
EX31_RS07550: EX31_RS07550 - AraC family transcriptional regulator, at 325,446 to 326,315
_RS07550
Position (kb) 324
325 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 323.177 kb on + strand, within EX31_RS07535 at 323.300 kb on + strand, within EX31_RS07535 at 323.368 kb on - strand at 323.391 kb on - strand at 323.402 kb on + strand at 323.403 kb on - strand at 323.515 kb on - strand at 323.537 kb on + strand at 323.538 kb on - strand at 323.717 kb on + strand at 323.718 kb on - strand at 323.718 kb on - strand at 323.768 kb on + strand, within EX31_RS07540 at 323.768 kb on + strand, within EX31_RS07540 at 323.769 kb on - strand, within EX31_RS07540 at 323.769 kb on - strand, within EX31_RS07540 at 323.769 kb on - strand, within EX31_RS07540 at 323.769 kb on - strand, within EX31_RS07540 at 323.841 kb on + strand, within EX31_RS07540 at 323.923 kb on + strand, within EX31_RS07540 at 323.980 kb on + strand, within EX31_RS07540 at 323.994 kb on + strand, within EX31_RS07540 at 323.995 kb on - strand, within EX31_RS07540 at 323.995 kb on - strand, within EX31_RS07540 at 324.032 kb on - strand at 324.032 kb on - strand at 324.052 kb on - strand at 324.073 kb on - strand at 324.073 kb on - strand at 324.075 kb on - strand at 324.075 kb on - strand at 324.088 kb on + strand at 324.088 kb on + strand at 324.088 kb on + strand at 324.088 kb on + strand at 324.088 kb on + strand at 324.089 kb on - strand at 324.089 kb on - strand at 324.089 kb on - strand at 324.091 kb on + strand at 324.091 kb on + strand at 324.091 kb on + strand at 324.092 kb on - strand at 324.092 kb on - strand at 324.093 kb on + strand at 324.093 kb on + strand at 324.097 kb on + strand at 324.118 kb on - strand at 324.159 kb on + strand at 324.159 kb on + strand at 324.159 kb on + strand at 324.176 kb on + strand at 324.198 kb on - strand at 324.294 kb on - strand, within EX31_RS07545 at 324.357 kb on - strand, within EX31_RS07545 at 324.387 kb on + strand, within EX31_RS07545 at 324.566 kb on + strand, within EX31_RS07545 at 324.567 kb on - strand, within EX31_RS07545 at 324.569 kb on + strand, within EX31_RS07545 at 324.570 kb on - strand, within EX31_RS07545 at 324.590 kb on + strand, within EX31_RS07545 at 324.590 kb on + strand, within EX31_RS07545 at 324.591 kb on - strand, within EX31_RS07545 at 324.690 kb on + strand, within EX31_RS07545 at 324.723 kb on + strand, within EX31_RS07545 at 324.724 kb on - strand, within EX31_RS07545 at 324.749 kb on + strand, within EX31_RS07545 at 324.773 kb on + strand, within EX31_RS07545 at 324.774 kb on - strand, within EX31_RS07545 at 324.818 kb on + strand, within EX31_RS07545 at 324.847 kb on + strand at 324.847 kb on + strand at 324.847 kb on + strand at 324.847 kb on + strand at 324.847 kb on + strand at 324.847 kb on + strand at 324.848 kb on - strand at 324.848 kb on - strand at 324.848 kb on - strand at 324.849 kb on + strand at 324.849 kb on + strand at 324.849 kb on + strand at 324.849 kb on + strand at 324.849 kb on + strand at 324.849 kb on + strand at 324.851 kb on + strand at 324.851 kb on + strand at 324.852 kb on - strand at 324.901 kb on + strand at 324.901 kb on + strand at 325.038 kb on - strand, within EX31_RS25970 at 325.069 kb on + strand, within EX31_RS25970 at 325.078 kb on + strand, within EX31_RS25970 at 325.080 kb on + strand, within EX31_RS25970 at 325.080 kb on + strand, within EX31_RS25970 at 325.080 kb on + strand, within EX31_RS25970 at 325.080 kb on + strand, within EX31_RS25970 at 325.080 kb on + strand, within EX31_RS25970 at 325.080 kb on + strand, within EX31_RS25970 at 325.081 kb on - strand, within EX31_RS25970 at 325.081 kb on - strand, within EX31_RS25970 at 325.081 kb on - strand, within EX31_RS25970 at 325.151 kb on + strand, within EX31_RS25230 at 325.151 kb on + strand, within EX31_RS25230 at 325.151 kb on + strand, within EX31_RS25230 at 325.157 kb on + strand, within EX31_RS25230 at 325.158 kb on - strand, within EX31_RS25230 at 325.281 kb on + strand, within EX31_RS25230 at 325.281 kb on + strand, within EX31_RS25230 at 325.282 kb on - strand, within EX31_RS25230 at 325.283 kb on + strand, within EX31_RS25230 at 325.283 kb on + strand, within EX31_RS25230 at 325.283 kb on + strand, within EX31_RS25230 at 325.283 kb on + strand, within EX31_RS25230 at 325.283 kb on + strand, within EX31_RS25230 at 325.284 kb on - strand, within EX31_RS25230 at 325.414 kb on - strand at 325.434 kb on - strand at 325.584 kb on + strand, within EX31_RS07550 at 325.628 kb on - strand, within EX31_RS07550 at 325.669 kb on + strand, within EX31_RS07550 at 325.809 kb on - strand, within EX31_RS07550 at 325.897 kb on - strand, within EX31_RS07550 at 325.897 kb on - strand, within EX31_RS07550
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 323,177 + EX31_RS07535 0.61 +0.0 323,300 + EX31_RS07535 0.83 +0.6 323,368 - +0.6 323,391 - +0.1 323,402 + +0.1 323,403 - +2.9 323,515 - -2.1 323,537 + +1.3 323,538 - +0.3 323,717 + +0.7 323,718 - +1.3 323,718 - +0.1 323,768 + EX31_RS07540 0.21 +0.8 323,768 + EX31_RS07540 0.21 +2.5 323,769 - EX31_RS07540 0.21 +0.5 323,769 - EX31_RS07540 0.21 +0.7 323,769 - EX31_RS07540 0.21 -0.7 323,769 - EX31_RS07540 0.21 -0.5 323,841 + EX31_RS07540 0.40 -0.4 323,923 + EX31_RS07540 0.62 +0.6 323,980 + EX31_RS07540 0.77 +0.9 323,994 + EX31_RS07540 0.81 -0.1 323,995 - EX31_RS07540 0.81 -0.3 323,995 - EX31_RS07540 0.81 -1.1 324,032 - +4.2 324,032 - +0.3 324,052 - +0.6 324,073 - +0.1 324,073 - +1.9 324,075 - -0.1 324,075 - +1.9 324,088 + +0.9 324,088 + -0.1 324,088 + +0.9 324,088 + +0.5 324,088 + +0.5 324,089 - +0.6 324,089 - +1.5 324,089 - -0.1 324,091 + -1.8 324,091 + +0.3 324,091 + +0.2 324,092 - -0.4 324,092 - -0.3 324,093 + +0.9 324,093 + +0.3 324,097 + -0.6 324,118 - +1.3 324,159 + -0.4 324,159 + +0.8 324,159 + -1.0 324,176 + -0.7 324,198 - +0.3 324,294 - EX31_RS07545 0.17 +0.6 324,357 - EX31_RS07545 0.26 -0.1 324,387 + EX31_RS07545 0.30 +0.7 324,566 + EX31_RS07545 0.55 +1.4 324,567 - EX31_RS07545 0.55 +0.7 324,569 + EX31_RS07545 0.55 +0.7 324,570 - EX31_RS07545 0.55 +0.9 324,590 + EX31_RS07545 0.58 -0.3 324,590 + EX31_RS07545 0.58 +0.9 324,591 - EX31_RS07545 0.58 +2.3 324,690 + EX31_RS07545 0.72 +1.2 324,723 + EX31_RS07545 0.76 -0.7 324,724 - EX31_RS07545 0.76 +0.3 324,749 + EX31_RS07545 0.80 +1.7 324,773 + EX31_RS07545 0.83 -1.1 324,774 - EX31_RS07545 0.83 +1.3 324,818 + EX31_RS07545 0.89 +1.6 324,847 + +0.5 324,847 + -1.1 324,847 + -0.4 324,847 + +0.0 324,847 + +0.0 324,847 + -1.1 324,848 - -0.2 324,848 - -0.3 324,848 - +0.6 324,849 + +0.8 324,849 + -1.2 324,849 + -0.7 324,849 + +1.9 324,849 + -0.7 324,849 + +1.9 324,851 + +1.9 324,851 + +0.5 324,852 - +0.5 324,901 + -0.7 324,901 + -0.4 325,038 - EX31_RS25970 0.20 +0.1 325,069 + EX31_RS25970 0.49 -2.7 325,078 + EX31_RS25970 0.57 +0.9 325,080 + EX31_RS25970 0.59 -0.4 325,080 + EX31_RS25970 0.59 -1.1 325,080 + EX31_RS25970 0.59 +1.2 325,080 + EX31_RS25970 0.59 +0.0 325,080 + EX31_RS25970 0.59 +0.7 325,080 + EX31_RS25970 0.59 +0.3 325,081 - EX31_RS25970 0.60 -0.3 325,081 - EX31_RS25970 0.60 +0.9 325,081 - EX31_RS25970 0.60 +1.2 325,151 + EX31_RS25230 0.11 +0.9 325,151 + EX31_RS25230 0.11 -1.1 325,151 + EX31_RS25230 0.11 +0.5 325,157 + EX31_RS25230 0.13 +1.0 325,158 - EX31_RS25230 0.14 +0.9 325,281 + EX31_RS25230 0.62 +1.6 325,281 + EX31_RS25230 0.62 -1.4 325,282 - EX31_RS25230 0.62 -1.7 325,283 + EX31_RS25230 0.63 -0.2 325,283 + EX31_RS25230 0.63 -0.1 325,283 + EX31_RS25230 0.63 +1.5 325,283 + EX31_RS25230 0.63 -0.6 325,283 + EX31_RS25230 0.63 -0.1 325,284 - EX31_RS25230 0.63 -1.6 325,414 - +0.9 325,434 - +0.5 325,584 + EX31_RS07550 0.16 -1.3 325,628 - EX31_RS07550 0.21 -0.7 325,669 + EX31_RS07550 0.26 +0.4 325,809 - EX31_RS07550 0.42 +0.2 325,897 - EX31_RS07550 0.52 +0.3 325,897 - EX31_RS07550 0.52 -0.1
Or see this region's nucleotide sequence