Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06740 and EX31_RS06745 are separated by 17 nucleotides EX31_RS06745 and EX31_RS06750 overlap by 1 nucleotides EX31_RS06750 and EX31_RS06755 are separated by 123 nucleotides
EX31_RS06740: EX31_RS06740 - ABC transporter substrate-binding protein, at 133,158 to 134,138
_RS06740
EX31_RS06745: EX31_RS06745 - ABC transporter permease, at 134,156 to 135,037
_RS06745
EX31_RS06750: EX31_RS06750 - ABC transporter ATP-binding protein, at 135,037 to 135,822
_RS06750
EX31_RS06755: EX31_RS06755 - histidine ammonia-lyase, at 135,946 to 137,496
_RS06755
Position (kb)
135
136 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 134.191 kb on + strand at 134.191 kb on + strand at 134.191 kb on + strand at 134.191 kb on + strand at 134.192 kb on - strand at 134.192 kb on - strand at 134.192 kb on - strand at 134.192 kb on - strand at 134.192 kb on - strand at 134.254 kb on + strand, within EX31_RS06745 at 134.421 kb on + strand, within EX31_RS06745 at 134.452 kb on + strand, within EX31_RS06745 at 134.453 kb on - strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.454 kb on + strand, within EX31_RS06745 at 134.455 kb on - strand, within EX31_RS06745 at 134.455 kb on - strand, within EX31_RS06745 at 134.455 kb on - strand, within EX31_RS06745 at 134.455 kb on - strand, within EX31_RS06745 at 134.458 kb on + strand, within EX31_RS06745 at 134.458 kb on + strand, within EX31_RS06745 at 134.458 kb on + strand, within EX31_RS06745 at 134.458 kb on + strand, within EX31_RS06745 at 134.458 kb on + strand, within EX31_RS06745 at 134.458 kb on + strand, within EX31_RS06745 at 134.459 kb on - strand, within EX31_RS06745 at 134.459 kb on - strand, within EX31_RS06745 at 134.459 kb on - strand, within EX31_RS06745 at 134.489 kb on - strand, within EX31_RS06745 at 134.537 kb on + strand, within EX31_RS06745 at 134.537 kb on + strand, within EX31_RS06745 at 134.537 kb on + strand, within EX31_RS06745 at 134.538 kb on - strand, within EX31_RS06745 at 134.585 kb on + strand, within EX31_RS06745 at 134.632 kb on + strand, within EX31_RS06745 at 134.744 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.852 kb on + strand, within EX31_RS06745 at 134.853 kb on - strand, within EX31_RS06745 at 134.853 kb on - strand, within EX31_RS06745 at 134.853 kb on - strand, within EX31_RS06745 at 134.853 kb on - strand, within EX31_RS06745 at 134.853 kb on - strand, within EX31_RS06745 at 134.953 kb on + strand at 135.076 kb on - strand at 135.180 kb on + strand, within EX31_RS06750 at 135.181 kb on - strand, within EX31_RS06750 at 135.298 kb on + strand, within EX31_RS06750 at 135.299 kb on - strand, within EX31_RS06750 at 135.299 kb on - strand, within EX31_RS06750 at 135.300 kb on + strand, within EX31_RS06750 at 135.301 kb on - strand, within EX31_RS06750 at 135.394 kb on + strand, within EX31_RS06750 at 135.395 kb on - strand, within EX31_RS06750 at 135.399 kb on + strand, within EX31_RS06750 at 135.400 kb on - strand, within EX31_RS06750 at 135.402 kb on + strand, within EX31_RS06750 at 135.402 kb on + strand, within EX31_RS06750 at 135.403 kb on - strand, within EX31_RS06750 at 135.405 kb on + strand, within EX31_RS06750 at 135.405 kb on + strand, within EX31_RS06750 at 135.651 kb on + strand, within EX31_RS06750 at 135.654 kb on + strand, within EX31_RS06750 at 135.655 kb on - strand, within EX31_RS06750 at 135.730 kb on + strand, within EX31_RS06750 at 135.730 kb on + strand, within EX31_RS06750 at 135.850 kb on - strand at 135.856 kb on + strand at 135.857 kb on - strand at 135.857 kb on - strand at 135.869 kb on + strand at 135.869 kb on + strand at 135.876 kb on + strand at 135.876 kb on + strand at 135.877 kb on - strand at 135.930 kb on + strand at 135.932 kb on + strand at 136.126 kb on + strand, within EX31_RS06755 at 136.406 kb on + strand, within EX31_RS06755 at 136.406 kb on + strand, within EX31_RS06755 at 136.820 kb on + strand, within EX31_RS06755
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 134,191 + +0.2 134,191 + -0.4 134,191 + +0.3 134,191 + +0.5 134,192 - -0.6 134,192 - +1.3 134,192 - +1.3 134,192 - +0.9 134,192 - -0.9 134,254 + EX31_RS06745 0.11 +1.7 134,421 + EX31_RS06745 0.30 -1.4 134,452 + EX31_RS06745 0.34 +0.4 134,453 - EX31_RS06745 0.34 +0.6 134,454 + EX31_RS06745 0.34 -1.4 134,454 + EX31_RS06745 0.34 +0.3 134,454 + EX31_RS06745 0.34 -1.9 134,454 + EX31_RS06745 0.34 -1.7 134,454 + EX31_RS06745 0.34 +1.1 134,454 + EX31_RS06745 0.34 +1.2 134,454 + EX31_RS06745 0.34 +0.1 134,454 + EX31_RS06745 0.34 -0.0 134,454 + EX31_RS06745 0.34 -0.3 134,454 + EX31_RS06745 0.34 +0.1 134,454 + EX31_RS06745 0.34 +0.6 134,454 + EX31_RS06745 0.34 +1.1 134,454 + EX31_RS06745 0.34 -0.4 134,454 + EX31_RS06745 0.34 +0.9 134,454 + EX31_RS06745 0.34 +0.5 134,454 + EX31_RS06745 0.34 +0.9 134,455 - EX31_RS06745 0.34 -0.9 134,455 - EX31_RS06745 0.34 -0.1 134,455 - EX31_RS06745 0.34 -0.1 134,455 - EX31_RS06745 0.34 +2.9 134,458 + EX31_RS06745 0.34 -0.8 134,458 + EX31_RS06745 0.34 +0.9 134,458 + EX31_RS06745 0.34 -0.7 134,458 + EX31_RS06745 0.34 -0.7 134,458 + EX31_RS06745 0.34 +2.5 134,458 + EX31_RS06745 0.34 +0.1 134,459 - EX31_RS06745 0.34 +0.2 134,459 - EX31_RS06745 0.34 +2.2 134,459 - EX31_RS06745 0.34 +0.2 134,489 - EX31_RS06745 0.38 +0.0 134,537 + EX31_RS06745 0.43 +1.0 134,537 + EX31_RS06745 0.43 -0.4 134,537 + EX31_RS06745 0.43 +0.6 134,538 - EX31_RS06745 0.43 -0.6 134,585 + EX31_RS06745 0.49 -1.7 134,632 + EX31_RS06745 0.54 -1.0 134,744 + EX31_RS06745 0.67 -1.0 134,852 + EX31_RS06745 0.79 -0.9 134,852 + EX31_RS06745 0.79 +0.0 134,852 + EX31_RS06745 0.79 +1.1 134,852 + EX31_RS06745 0.79 +0.7 134,852 + EX31_RS06745 0.79 +0.3 134,852 + EX31_RS06745 0.79 -0.2 134,852 + EX31_RS06745 0.79 -0.9 134,852 + EX31_RS06745 0.79 +0.3 134,852 + EX31_RS06745 0.79 +0.9 134,853 - EX31_RS06745 0.79 -0.2 134,853 - EX31_RS06745 0.79 +1.5 134,853 - EX31_RS06745 0.79 +0.5 134,853 - EX31_RS06745 0.79 -1.1 134,853 - EX31_RS06745 0.79 -1.1 134,953 + +1.5 135,076 - +1.2 135,180 + EX31_RS06750 0.18 +0.7 135,181 - EX31_RS06750 0.18 -0.1 135,298 + EX31_RS06750 0.33 -1.9 135,299 - EX31_RS06750 0.33 -0.2 135,299 - EX31_RS06750 0.33 +2.9 135,300 + EX31_RS06750 0.33 +2.5 135,301 - EX31_RS06750 0.34 +0.1 135,394 + EX31_RS06750 0.45 +0.9 135,395 - EX31_RS06750 0.46 +2.6 135,399 + EX31_RS06750 0.46 -0.4 135,400 - EX31_RS06750 0.46 +1.9 135,402 + EX31_RS06750 0.46 +0.5 135,402 + EX31_RS06750 0.46 -1.9 135,403 - EX31_RS06750 0.47 -0.9 135,405 + EX31_RS06750 0.47 +1.9 135,405 + EX31_RS06750 0.47 +2.9 135,651 + EX31_RS06750 0.78 -1.7 135,654 + EX31_RS06750 0.78 +0.3 135,655 - EX31_RS06750 0.79 -0.1 135,730 + EX31_RS06750 0.88 +1.3 135,730 + EX31_RS06750 0.88 +1.7 135,850 - +0.3 135,856 + -1.8 135,857 - -2.7 135,857 - +1.9 135,869 + -1.4 135,869 + +2.9 135,876 + +1.6 135,876 + +0.9 135,877 - +0.6 135,930 + -0.3 135,932 + -0.2 136,126 + EX31_RS06755 0.12 -0.7 136,406 + EX31_RS06755 0.30 -0.0 136,406 + EX31_RS06755 0.30 +1.4 136,820 + EX31_RS06755 0.56 +1.1
Or see this region's nucleotide sequence