Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06280 and EX31_RS06285 are separated by 340 nucleotides EX31_RS06285 and EX31_RS06290 are separated by 95 nucleotides EX31_RS06290 and EX31_RS06295 are separated by 76 nucleotides
EX31_RS06280: EX31_RS06280 - glyceraldehyde-3-phosphate dehydrogenase, at 18,750 to 19,757
_RS06280
EX31_RS06285: EX31_RS06285 - peptide-methionine (R)-S-oxide reductase MsrB, at 20,098 to 20,511
_RS06285
EX31_RS06290: EX31_RS06290 - DUF1315 family protein, at 20,607 to 20,879
_RS06290
EX31_RS06295: EX31_RS06295 - bifunctional nicotinamidase/pyrazinamidase, at 20,956 to 21,585
_RS06295
Position (kb) 20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 19.837 kb on + strand at 19.837 kb on + strand at 19.837 kb on + strand at 19.838 kb on - strand at 19.838 kb on - strand at 19.838 kb on - strand at 19.846 kb on - strand at 19.864 kb on - strand at 19.933 kb on + strand at 19.933 kb on + strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.935 kb on + strand at 19.935 kb on + strand at 19.935 kb on + strand at 19.935 kb on + strand at 19.935 kb on + strand at 19.936 kb on - strand at 19.936 kb on - strand at 19.936 kb on - strand at 20.002 kb on - strand at 20.008 kb on + strand at 20.008 kb on + strand at 20.049 kb on - strand at 20.058 kb on + strand at 20.058 kb on + strand at 20.227 kb on + strand, within EX31_RS06285 at 20.227 kb on + strand, within EX31_RS06285 at 20.227 kb on + strand, within EX31_RS06285 at 20.228 kb on - strand, within EX31_RS06285 at 20.260 kb on + strand, within EX31_RS06285 at 20.335 kb on + strand, within EX31_RS06285 at 20.446 kb on + strand, within EX31_RS06285 at 20.453 kb on + strand, within EX31_RS06285 at 20.476 kb on - strand at 20.532 kb on + strand at 20.532 kb on + strand at 20.583 kb on + strand at 20.615 kb on + strand at 20.615 kb on + strand at 20.615 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.624 kb on + strand at 20.632 kb on + strand at 20.632 kb on + strand at 20.632 kb on + strand at 20.632 kb on + strand at 20.632 kb on + strand at 20.632 kb on + strand at 20.749 kb on - strand, within EX31_RS06290 at 20.852 kb on + strand at 20.852 kb on + strand at 20.861 kb on + strand at 20.877 kb on + strand at 20.965 kb on + strand at 21.000 kb on + strand at 21.001 kb on - strand at 21.125 kb on + strand, within EX31_RS06295 at 21.126 kb on - strand, within EX31_RS06295 at 21.126 kb on - strand, within EX31_RS06295 at 21.145 kb on + strand, within EX31_RS06295 at 21.145 kb on + strand, within EX31_RS06295 at 21.146 kb on - strand, within EX31_RS06295 at 21.146 kb on - strand, within EX31_RS06295 at 21.146 kb on - strand, within EX31_RS06295 at 21.146 kb on - strand, within EX31_RS06295 at 21.146 kb on - strand, within EX31_RS06295 at 21.149 kb on + strand, within EX31_RS06295 at 21.149 kb on + strand, within EX31_RS06295 at 21.149 kb on + strand, within EX31_RS06295 at 21.149 kb on + strand, within EX31_RS06295 at 21.149 kb on + strand, within EX31_RS06295 at 21.150 kb on - strand, within EX31_RS06295 at 21.150 kb on - strand, within EX31_RS06295 at 21.150 kb on - strand, within EX31_RS06295 at 21.150 kb on - strand, within EX31_RS06295 at 21.150 kb on - strand, within EX31_RS06295 at 21.150 kb on - strand, within EX31_RS06295 at 21.160 kb on - strand, within EX31_RS06295 at 21.230 kb on + strand, within EX31_RS06295 at 21.230 kb on + strand, within EX31_RS06295 at 21.230 kb on + strand, within EX31_RS06295 at 21.230 kb on + strand, within EX31_RS06295 at 21.230 kb on + strand, within EX31_RS06295 at 21.231 kb on - strand, within EX31_RS06295 at 21.231 kb on - strand, within EX31_RS06295 at 21.468 kb on + strand, within EX31_RS06295 at 21.469 kb on - strand, within EX31_RS06295 at 21.469 kb on - strand, within EX31_RS06295
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 19,837 + +1.9 19,837 + -1.1 19,837 + +0.6 19,838 - -1.4 19,838 - +0.5 19,838 - -0.8 19,846 - -3.0 19,864 - +0.8 19,933 + +1.9 19,933 + +0.3 19,934 - +1.9 19,934 - -2.1 19,934 - -0.4 19,934 - +0.6 19,934 - +1.2 19,935 + +0.2 19,935 + -0.8 19,935 + -0.7 19,935 + -0.7 19,935 + +1.5 19,936 - -3.0 19,936 - +1.3 19,936 - -1.6 20,002 - +0.5 20,008 + +0.1 20,008 + +0.8 20,049 - -0.8 20,058 + +0.9 20,058 + +0.5 20,227 + EX31_RS06285 0.31 +1.3 20,227 + EX31_RS06285 0.31 -1.4 20,227 + EX31_RS06285 0.31 -0.1 20,228 - EX31_RS06285 0.31 +1.9 20,260 + EX31_RS06285 0.39 +1.5 20,335 + EX31_RS06285 0.57 +1.5 20,446 + EX31_RS06285 0.84 +1.2 20,453 + EX31_RS06285 0.86 -0.4 20,476 - +1.9 20,532 + +0.8 20,532 + +0.4 20,583 + +0.3 20,615 + +0.2 20,615 + -0.1 20,615 + -0.2 20,624 + -2.2 20,624 + -0.3 20,624 + -0.6 20,624 + +0.4 20,624 + -0.1 20,624 + +0.3 20,624 + -0.7 20,624 + -0.1 20,632 + +1.9 20,632 + -0.3 20,632 + -0.7 20,632 + +0.4 20,632 + -0.4 20,632 + -0.3 20,749 - EX31_RS06290 0.52 +0.3 20,852 + +0.7 20,852 + -0.2 20,861 + +2.2 20,877 + +0.4 20,965 + +0.3 21,000 + +0.6 21,001 - -1.1 21,125 + EX31_RS06295 0.27 +2.6 21,126 - EX31_RS06295 0.27 +2.1 21,126 - EX31_RS06295 0.27 +1.1 21,145 + EX31_RS06295 0.30 -0.2 21,145 + EX31_RS06295 0.30 +1.4 21,146 - EX31_RS06295 0.30 +0.3 21,146 - EX31_RS06295 0.30 +0.1 21,146 - EX31_RS06295 0.30 +0.3 21,146 - EX31_RS06295 0.30 -0.4 21,146 - EX31_RS06295 0.30 +1.9 21,149 + EX31_RS06295 0.31 +0.1 21,149 + EX31_RS06295 0.31 +0.1 21,149 + EX31_RS06295 0.31 -0.9 21,149 + EX31_RS06295 0.31 -0.2 21,149 + EX31_RS06295 0.31 -0.6 21,150 - EX31_RS06295 0.31 +1.2 21,150 - EX31_RS06295 0.31 -0.2 21,150 - EX31_RS06295 0.31 +0.3 21,150 - EX31_RS06295 0.31 +1.2 21,150 - EX31_RS06295 0.31 +2.3 21,150 - EX31_RS06295 0.31 -0.9 21,160 - EX31_RS06295 0.32 +0.1 21,230 + EX31_RS06295 0.43 +0.8 21,230 + EX31_RS06295 0.43 -2.7 21,230 + EX31_RS06295 0.43 +0.3 21,230 + EX31_RS06295 0.43 +0.1 21,230 + EX31_RS06295 0.43 -2.8 21,231 - EX31_RS06295 0.44 +1.3 21,231 - EX31_RS06295 0.44 -0.4 21,468 + EX31_RS06295 0.81 +0.8 21,469 - EX31_RS06295 0.81 -0.4 21,469 - EX31_RS06295 0.81 -0.9
Or see this region's nucleotide sequence