Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03700 and EX31_RS03705 are separated by 227 nucleotides EX31_RS03705 and EX31_RS03710 are separated by 160 nucleotides EX31_RS03710 and EX31_RS03715 are separated by 102 nucleotides
EX31_RS03700: EX31_RS03700 - hypothetical protein, at 240,372 to 241,064
_RS03700
EX31_RS03705: EX31_RS03705 - MFS transporter, at 241,292 to 242,455
_RS03705
EX31_RS03710: EX31_RS03710 - glucose uptake inhibitor SgrT, at 242,616 to 242,801
_RS03710
EX31_RS03715: EX31_RS03715 - HTH-type transcriptional regulator SgrR, at 242,904 to 244,565
_RS03715
Position (kb) 241
242
243 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 240.307 kb on - strand at 240.325 kb on - strand at 240.437 kb on - strand at 240.446 kb on + strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.524 kb on - strand, within EX31_RS03700 at 240.635 kb on + strand, within EX31_RS03700 at 240.639 kb on + strand, within EX31_RS03700 at 240.639 kb on + strand, within EX31_RS03700 at 240.639 kb on + strand, within EX31_RS03700 at 240.640 kb on - strand, within EX31_RS03700 at 240.640 kb on - strand, within EX31_RS03700 at 240.654 kb on - strand, within EX31_RS03700 at 240.654 kb on - strand, within EX31_RS03700 at 240.654 kb on - strand, within EX31_RS03700 at 240.675 kb on + strand, within EX31_RS03700 at 240.728 kb on - strand, within EX31_RS03700 at 240.799 kb on + strand, within EX31_RS03700 at 240.799 kb on + strand, within EX31_RS03700 at 240.800 kb on - strand, within EX31_RS03700 at 240.800 kb on - strand, within EX31_RS03700 at 240.800 kb on - strand, within EX31_RS03700 at 240.865 kb on - strand, within EX31_RS03700 at 240.943 kb on - strand, within EX31_RS03700 at 240.961 kb on - strand, within EX31_RS03700 at 240.961 kb on - strand, within EX31_RS03700 at 240.961 kb on - strand, within EX31_RS03700 at 240.963 kb on - strand, within EX31_RS03700 at 241.031 kb on + strand at 241.032 kb on - strand at 241.046 kb on + strand at 241.050 kb on + strand at 241.101 kb on + strand at 241.104 kb on + strand at 241.130 kb on - strand at 241.136 kb on - strand at 241.201 kb on + strand at 241.233 kb on + strand at 241.235 kb on + strand at 241.235 kb on + strand at 241.235 kb on + strand at 241.250 kb on + strand at 241.284 kb on + strand at 241.285 kb on - strand at 241.285 kb on - strand at 241.285 kb on - strand at 241.285 kb on - strand at 241.293 kb on + strand at 241.309 kb on + strand at 241.310 kb on - strand at 241.310 kb on - strand at 241.310 kb on - strand at 241.310 kb on - strand at 241.311 kb on + strand at 241.311 kb on + strand at 241.311 kb on + strand at 241.311 kb on + strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.356 kb on + strand at 241.388 kb on - strand at 241.442 kb on + strand, within EX31_RS03705 at 241.448 kb on - strand, within EX31_RS03705 at 241.488 kb on + strand, within EX31_RS03705 at 241.488 kb on + strand, within EX31_RS03705 at 241.488 kb on + strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.587 kb on + strand, within EX31_RS03705 at 241.614 kb on + strand, within EX31_RS03705 at 241.614 kb on + strand, within EX31_RS03705 at 241.615 kb on - strand, within EX31_RS03705 at 241.653 kb on + strand, within EX31_RS03705 at 241.749 kb on + strand, within EX31_RS03705 at 241.749 kb on + strand, within EX31_RS03705 at 241.750 kb on - strand, within EX31_RS03705 at 241.750 kb on - strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.887 kb on - strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.917 kb on - strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.968 kb on + strand, within EX31_RS03705 at 241.968 kb on + strand, within EX31_RS03705 at 241.969 kb on - strand, within EX31_RS03705 at 241.969 kb on - strand, within EX31_RS03705 at 241.969 kb on - strand, within EX31_RS03705 at 242.123 kb on + strand, within EX31_RS03705 at 242.124 kb on - strand, within EX31_RS03705 at 242.124 kb on - strand, within EX31_RS03705 at 242.163 kb on + strand, within EX31_RS03705 at 242.164 kb on - strand, within EX31_RS03705 at 242.305 kb on + strand, within EX31_RS03705 at 242.305 kb on + strand, within EX31_RS03705 at 242.305 kb on + strand, within EX31_RS03705 at 242.306 kb on - strand, within EX31_RS03705 at 242.306 kb on - strand, within EX31_RS03705 at 242.306 kb on - strand, within EX31_RS03705 at 242.306 kb on - strand, within EX31_RS03705 at 242.307 kb on + strand, within EX31_RS03705 at 242.307 kb on + strand, within EX31_RS03705 at 242.307 kb on + strand, within EX31_RS03705 at 242.307 kb on + strand, within EX31_RS03705 at 242.308 kb on - strand, within EX31_RS03705 at 242.308 kb on - strand, within EX31_RS03705 at 242.308 kb on - strand, within EX31_RS03705 at 242.308 kb on - strand, within EX31_RS03705 at 242.379 kb on - strand at 242.379 kb on - strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.427 kb on + strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.428 kb on - strand at 242.431 kb on + strand at 242.431 kb on + strand at 242.431 kb on + strand at 242.431 kb on + strand at 242.432 kb on - strand at 242.437 kb on + strand at 242.437 kb on + strand at 242.438 kb on - strand at 242.485 kb on - strand at 242.485 kb on - strand at 242.504 kb on - strand at 242.575 kb on + strand at 242.575 kb on + strand at 242.575 kb on + strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.576 kb on - strand at 242.583 kb on + strand at 242.584 kb on - strand at 242.584 kb on - strand at 242.601 kb on + strand at 242.684 kb on + strand, within EX31_RS03710 at 242.854 kb on - strand at 242.854 kb on - strand at 242.856 kb on + strand at 242.857 kb on - strand at 242.869 kb on - strand at 242.903 kb on + strand at 242.904 kb on - strand at 242.980 kb on + strand at 242.981 kb on - strand at 242.981 kb on - strand at 242.981 kb on - strand at 242.981 kb on - strand at 243.114 kb on + strand, within EX31_RS03715 at 243.114 kb on + strand, within EX31_RS03715 at 243.116 kb on + strand, within EX31_RS03715 at 243.116 kb on + strand, within EX31_RS03715 at 243.117 kb on - strand, within EX31_RS03715 at 243.184 kb on + strand, within EX31_RS03715 at 243.185 kb on - strand, within EX31_RS03715 at 243.185 kb on - strand, within EX31_RS03715 at 243.263 kb on + strand, within EX31_RS03715 at 243.263 kb on + strand, within EX31_RS03715 at 243.263 kb on + strand, within EX31_RS03715 at 243.264 kb on - strand, within EX31_RS03715 at 243.264 kb on - strand, within EX31_RS03715 at 243.264 kb on - strand, within EX31_RS03715 at 243.279 kb on + strand, within EX31_RS03715 at 243.279 kb on + strand, within EX31_RS03715 at 243.279 kb on + strand, within EX31_RS03715 at 243.279 kb on + strand, within EX31_RS03715 at 243.279 kb on + strand, within EX31_RS03715 at 243.280 kb on - strand, within EX31_RS03715 at 243.280 kb on - strand, within EX31_RS03715 at 243.373 kb on + strand, within EX31_RS03715 at 243.373 kb on + strand, within EX31_RS03715 at 243.373 kb on + strand, within EX31_RS03715 at 243.373 kb on + strand, within EX31_RS03715 at 243.374 kb on - strand, within EX31_RS03715 at 243.374 kb on - strand, within EX31_RS03715 at 243.374 kb on - strand, within EX31_RS03715 at 243.374 kb on - strand, within EX31_RS03715 at 243.450 kb on + strand, within EX31_RS03715 at 243.451 kb on - strand, within EX31_RS03715
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 240,307 - +0.9 240,325 - +0.6 240,437 - -0.2 240,446 + EX31_RS03700 0.11 -0.0 240,456 - EX31_RS03700 0.12 +1.1 240,456 - EX31_RS03700 0.12 +0.0 240,456 - EX31_RS03700 0.12 -0.5 240,524 - EX31_RS03700 0.22 +0.3 240,635 + EX31_RS03700 0.38 -1.4 240,639 + EX31_RS03700 0.39 -0.7 240,639 + EX31_RS03700 0.39 -1.3 240,639 + EX31_RS03700 0.39 -1.1 240,640 - EX31_RS03700 0.39 +0.9 240,640 - EX31_RS03700 0.39 +0.4 240,654 - EX31_RS03700 0.41 -0.1 240,654 - EX31_RS03700 0.41 +0.3 240,654 - EX31_RS03700 0.41 -0.1 240,675 + EX31_RS03700 0.44 +0.2 240,728 - EX31_RS03700 0.51 +0.1 240,799 + EX31_RS03700 0.62 +1.0 240,799 + EX31_RS03700 0.62 +0.1 240,800 - EX31_RS03700 0.62 -0.6 240,800 - EX31_RS03700 0.62 +0.2 240,800 - EX31_RS03700 0.62 -0.6 240,865 - EX31_RS03700 0.71 -0.1 240,943 - EX31_RS03700 0.82 +0.9 240,961 - EX31_RS03700 0.85 +0.5 240,961 - EX31_RS03700 0.85 +0.2 240,961 - EX31_RS03700 0.85 -1.0 240,963 - EX31_RS03700 0.85 +0.1 241,031 + +0.8 241,032 - -0.8 241,046 + +0.1 241,050 + -0.9 241,101 + -0.5 241,104 + -0.5 241,130 - +0.1 241,136 - -0.8 241,201 + -0.3 241,233 + +0.1 241,235 + +0.1 241,235 + +0.6 241,235 + +0.2 241,250 + -0.6 241,284 + +0.2 241,285 - -1.4 241,285 - +0.6 241,285 - -0.6 241,285 - +1.2 241,293 + +0.4 241,309 + -0.7 241,310 - -0.7 241,310 - +0.2 241,310 - +0.7 241,310 - -0.8 241,311 + +0.9 241,311 + -0.2 241,311 + +0.5 241,311 + +1.8 241,312 - -0.2 241,312 - +0.9 241,312 - -1.3 241,312 - -0.7 241,312 - +0.4 241,356 + -0.5 241,388 - +0.3 241,442 + EX31_RS03705 0.13 +0.6 241,448 - EX31_RS03705 0.13 +2.2 241,488 + EX31_RS03705 0.17 +0.3 241,488 + EX31_RS03705 0.17 -0.1 241,488 + EX31_RS03705 0.17 +0.3 241,489 - EX31_RS03705 0.17 +0.1 241,489 - EX31_RS03705 0.17 +0.5 241,489 - EX31_RS03705 0.17 -0.2 241,489 - EX31_RS03705 0.17 -0.3 241,587 + EX31_RS03705 0.25 +0.2 241,614 + EX31_RS03705 0.28 +0.4 241,614 + EX31_RS03705 0.28 +0.1 241,615 - EX31_RS03705 0.28 +0.7 241,653 + EX31_RS03705 0.31 -0.5 241,749 + EX31_RS03705 0.39 +0.3 241,749 + EX31_RS03705 0.39 -0.9 241,750 - EX31_RS03705 0.39 +0.7 241,750 - EX31_RS03705 0.39 -1.0 241,770 + EX31_RS03705 0.41 -0.1 241,770 + EX31_RS03705 0.41 -0.6 241,770 + EX31_RS03705 0.41 +0.2 241,770 + EX31_RS03705 0.41 -1.0 241,771 - EX31_RS03705 0.41 -1.7 241,771 - EX31_RS03705 0.41 +0.8 241,771 - EX31_RS03705 0.41 -0.8 241,771 - EX31_RS03705 0.41 -0.2 241,771 - EX31_RS03705 0.41 -1.4 241,771 - EX31_RS03705 0.41 +0.2 241,771 - EX31_RS03705 0.41 -0.6 241,771 - EX31_RS03705 0.41 -0.3 241,771 - EX31_RS03705 0.41 -0.5 241,887 - EX31_RS03705 0.51 +0.7 241,916 + EX31_RS03705 0.54 +0.4 241,916 + EX31_RS03705 0.54 +1.5 241,916 + EX31_RS03705 0.54 -1.3 241,916 + EX31_RS03705 0.54 +0.0 241,917 - EX31_RS03705 0.54 -0.2 241,950 + EX31_RS03705 0.57 +0.0 241,950 + EX31_RS03705 0.57 -0.2 241,950 + EX31_RS03705 0.57 +0.3 241,950 + EX31_RS03705 0.57 +0.8 241,950 + EX31_RS03705 0.57 -0.2 241,950 + EX31_RS03705 0.57 -0.3 241,950 + EX31_RS03705 0.57 +0.0 241,950 + EX31_RS03705 0.57 +0.7 241,951 - EX31_RS03705 0.57 +0.3 241,951 - EX31_RS03705 0.57 -0.8 241,951 - EX31_RS03705 0.57 +0.3 241,951 - EX31_RS03705 0.57 -0.2 241,951 - EX31_RS03705 0.57 -1.7 241,951 - EX31_RS03705 0.57 +0.4 241,951 - EX31_RS03705 0.57 +0.7 241,968 + EX31_RS03705 0.58 -0.1 241,968 + EX31_RS03705 0.58 -0.6 241,969 - EX31_RS03705 0.58 -0.7 241,969 - EX31_RS03705 0.58 +0.3 241,969 - EX31_RS03705 0.58 -0.4 242,123 + EX31_RS03705 0.71 +2.1 242,124 - EX31_RS03705 0.71 -0.2 242,124 - EX31_RS03705 0.71 +0.2 242,163 + EX31_RS03705 0.75 +0.5 242,164 - EX31_RS03705 0.75 +0.9 242,305 + EX31_RS03705 0.87 +0.5 242,305 + EX31_RS03705 0.87 -0.3 242,305 + EX31_RS03705 0.87 +1.5 242,306 - EX31_RS03705 0.87 -0.2 242,306 - EX31_RS03705 0.87 -0.7 242,306 - EX31_RS03705 0.87 +0.2 242,306 - EX31_RS03705 0.87 +0.6 242,307 + EX31_RS03705 0.87 -1.3 242,307 + EX31_RS03705 0.87 -0.0 242,307 + EX31_RS03705 0.87 +1.7 242,307 + EX31_RS03705 0.87 -0.1 242,308 - EX31_RS03705 0.87 +0.8 242,308 - EX31_RS03705 0.87 +0.4 242,308 - EX31_RS03705 0.87 -0.3 242,308 - EX31_RS03705 0.87 +0.1 242,379 - +0.3 242,379 - +1.3 242,427 + -0.4 242,427 + +1.0 242,427 + -0.5 242,427 + +0.3 242,427 + -0.1 242,427 + -0.1 242,427 + +0.4 242,427 + +1.1 242,427 + -0.7 242,427 + -0.2 242,427 + +2.9 242,427 + -0.5 242,428 - -2.5 242,428 - -0.5 242,428 - -1.2 242,428 - -0.2 242,428 - -1.2 242,428 - -0.0 242,428 - +0.8 242,428 - +0.8 242,428 - -1.1 242,428 - -0.6 242,428 - +0.2 242,428 - -0.0 242,428 - -0.2 242,431 + -0.3 242,431 + -0.5 242,431 + -0.1 242,431 + -1.7 242,432 - -0.2 242,437 + +0.9 242,437 + -0.7 242,438 - +1.0 242,485 - +0.5 242,485 - -0.3 242,504 - -0.9 242,575 + -0.2 242,575 + +1.2 242,575 + -0.1 242,576 - -1.2 242,576 - -0.0 242,576 - -0.6 242,576 - +0.4 242,576 - +0.3 242,576 - -0.2 242,576 - +0.5 242,576 - +0.3 242,576 - -1.5 242,583 + +0.6 242,584 - +0.8 242,584 - -0.8 242,601 + -0.5 242,684 + EX31_RS03710 0.37 -0.6 242,854 - +1.6 242,854 - -0.4 242,856 + -0.1 242,857 - -0.0 242,869 - -2.3 242,903 + -1.9 242,904 - -0.6 242,980 + -1.1 242,981 - +0.1 242,981 - +0.2 242,981 - -0.3 242,981 - +0.1 243,114 + EX31_RS03715 0.13 +0.5 243,114 + EX31_RS03715 0.13 +0.4 243,116 + EX31_RS03715 0.13 +0.9 243,116 + EX31_RS03715 0.13 -0.1 243,117 - EX31_RS03715 0.13 -1.3 243,184 + EX31_RS03715 0.17 -1.1 243,185 - EX31_RS03715 0.17 -0.5 243,185 - EX31_RS03715 0.17 -1.2 243,263 + EX31_RS03715 0.22 +0.9 243,263 + EX31_RS03715 0.22 -0.1 243,263 + EX31_RS03715 0.22 -0.8 243,264 - EX31_RS03715 0.22 -0.8 243,264 - EX31_RS03715 0.22 +0.2 243,264 - EX31_RS03715 0.22 -0.7 243,279 + EX31_RS03715 0.23 -0.0 243,279 + EX31_RS03715 0.23 +0.3 243,279 + EX31_RS03715 0.23 +0.4 243,279 + EX31_RS03715 0.23 -0.1 243,279 + EX31_RS03715 0.23 -0.4 243,280 - EX31_RS03715 0.23 -0.5 243,280 - EX31_RS03715 0.23 -0.5 243,373 + EX31_RS03715 0.28 -0.9 243,373 + EX31_RS03715 0.28 +0.3 243,373 + EX31_RS03715 0.28 +0.8 243,373 + EX31_RS03715 0.28 -0.8 243,374 - EX31_RS03715 0.28 +0.8 243,374 - EX31_RS03715 0.28 -1.1 243,374 - EX31_RS03715 0.28 -0.5 243,374 - EX31_RS03715 0.28 -0.5 243,450 + EX31_RS03715 0.33 +0.8 243,451 - EX31_RS03715 0.33 +1.2
Or see this region's nucleotide sequence