Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01195 and EX31_RS01200 are separated by 314 nucleotides EX31_RS01200 and EX31_RS01205 are separated by 205 nucleotides EX31_RS01205 and EX31_RS01210 are separated by 343 nucleotides
EX31_RS01195: EX31_RS01195 - SDR family oxidoreductase, at 264,793 to 265,698
_RS01195
EX31_RS01200: EX31_RS01200 - hypothetical protein, at 266,013 to 266,234
_RS01200
EX31_RS01205: EX31_RS01205 - MurR/RpiR family transcriptional regulator, at 266,440 to 267,348
_RS01205
EX31_RS01210: EX31_RS01210 - TonB-dependent siderophore receptor, at 267,692 to 269,821
_RS01210
Position (kb)
266
267
268 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 265.508 kb on - strand, within EX31_RS01195 at 265.618 kb on + strand at 265.618 kb on + strand at 265.618 kb on + strand at 265.618 kb on + strand at 265.619 kb on - strand at 265.619 kb on - strand at 265.619 kb on - strand at 265.694 kb on + strand at 265.695 kb on - strand at 265.695 kb on - strand at 265.697 kb on - strand at 265.697 kb on - strand at 265.697 kb on - strand at 265.779 kb on + strand at 265.783 kb on + strand at 265.784 kb on - strand at 265.791 kb on + strand at 265.800 kb on - strand at 265.819 kb on + strand at 265.819 kb on + strand at 265.820 kb on - strand at 265.831 kb on - strand at 265.843 kb on + strand at 265.965 kb on - strand at 266.096 kb on - strand, within EX31_RS01200 at 266.151 kb on - strand, within EX31_RS01200 at 266.151 kb on - strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.169 kb on + strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.170 kb on - strand, within EX31_RS01200 at 266.266 kb on + strand at 266.267 kb on - strand at 266.294 kb on + strand at 266.307 kb on - strand at 266.366 kb on - strand at 266.555 kb on - strand, within EX31_RS01205 at 266.641 kb on - strand, within EX31_RS01205 at 266.697 kb on + strand, within EX31_RS01205 at 266.698 kb on - strand, within EX31_RS01205 at 266.719 kb on + strand, within EX31_RS01205 at 266.720 kb on - strand, within EX31_RS01205 at 266.826 kb on + strand, within EX31_RS01205 at 266.826 kb on + strand, within EX31_RS01205 at 266.834 kb on - strand, within EX31_RS01205 at 266.874 kb on - strand, within EX31_RS01205 at 266.874 kb on - strand, within EX31_RS01205 at 266.874 kb on - strand, within EX31_RS01205 at 266.986 kb on - strand, within EX31_RS01205 at 267.000 kb on + strand, within EX31_RS01205 at 267.001 kb on - strand, within EX31_RS01205 at 267.163 kb on + strand, within EX31_RS01205 at 267.163 kb on + strand, within EX31_RS01205 at 267.167 kb on - strand, within EX31_RS01205 at 267.167 kb on - strand, within EX31_RS01205 at 267.172 kb on - strand, within EX31_RS01205 at 267.247 kb on - strand, within EX31_RS01205 at 267.247 kb on - strand, within EX31_RS01205 at 267.248 kb on + strand, within EX31_RS01205 at 267.248 kb on + strand, within EX31_RS01205 at 267.248 kb on + strand, within EX31_RS01205 at 267.248 kb on + strand, within EX31_RS01205 at 267.249 kb on - strand, within EX31_RS01205 at 267.249 kb on - strand, within EX31_RS01205 at 267.249 kb on - strand, within EX31_RS01205 at 267.249 kb on - strand, within EX31_RS01205 at 267.603 kb on + strand at 267.603 kb on + strand at 267.610 kb on + strand at 267.610 kb on + strand at 267.611 kb on - strand at 267.616 kb on + strand at 267.617 kb on - strand at 267.617 kb on - strand at 267.620 kb on + strand at 267.667 kb on + strand at 267.700 kb on + strand at 267.752 kb on - strand at 267.755 kb on + strand at 267.756 kb on - strand at 267.788 kb on + strand at 267.848 kb on + strand at 268.007 kb on - strand, within EX31_RS01210 at 268.007 kb on - strand, within EX31_RS01210 at 268.218 kb on - strand, within EX31_RS01210
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 265,508 - EX31_RS01195 0.79 -1.2 265,618 + +1.3 265,618 + -2.1 265,618 + +1.1 265,618 + +1.7 265,619 - -0.6 265,619 - -1.1 265,619 - -0.7 265,694 + +2.1 265,695 - -0.2 265,695 - +1.4 265,697 - -0.2 265,697 - -1.3 265,697 - -0.1 265,779 + -1.0 265,783 + +1.4 265,784 - -0.6 265,791 + +1.1 265,800 - -1.5 265,819 + -1.0 265,819 + +0.7 265,820 - -1.2 265,831 - +1.5 265,843 + +0.9 265,965 - +1.1 266,096 - EX31_RS01200 0.37 +0.2 266,151 - EX31_RS01200 0.62 -0.7 266,151 - EX31_RS01200 0.62 +0.2 266,169 + EX31_RS01200 0.70 +0.3 266,169 + EX31_RS01200 0.70 +0.4 266,169 + EX31_RS01200 0.70 -0.8 266,169 + EX31_RS01200 0.70 +0.0 266,169 + EX31_RS01200 0.70 +1.5 266,169 + EX31_RS01200 0.70 -1.2 266,169 + EX31_RS01200 0.70 -1.0 266,169 + EX31_RS01200 0.70 -1.0 266,170 - EX31_RS01200 0.71 +0.0 266,170 - EX31_RS01200 0.71 -0.5 266,170 - EX31_RS01200 0.71 +0.1 266,170 - EX31_RS01200 0.71 +0.7 266,170 - EX31_RS01200 0.71 +0.1 266,170 - EX31_RS01200 0.71 +0.1 266,170 - EX31_RS01200 0.71 +0.9 266,170 - EX31_RS01200 0.71 +0.2 266,170 - EX31_RS01200 0.71 +1.2 266,266 + +1.1 266,267 - -0.3 266,294 + +0.1 266,307 - -0.6 266,366 - -0.8 266,555 - EX31_RS01205 0.13 +1.8 266,641 - EX31_RS01205 0.22 -0.5 266,697 + EX31_RS01205 0.28 +0.5 266,698 - EX31_RS01205 0.28 +0.9 266,719 + EX31_RS01205 0.31 +1.4 266,720 - EX31_RS01205 0.31 +0.2 266,826 + EX31_RS01205 0.42 +1.0 266,826 + EX31_RS01205 0.42 -0.2 266,834 - EX31_RS01205 0.43 +0.4 266,874 - EX31_RS01205 0.48 +1.3 266,874 - EX31_RS01205 0.48 +1.2 266,874 - EX31_RS01205 0.48 +0.1 266,986 - EX31_RS01205 0.60 -1.2 267,000 + EX31_RS01205 0.62 +1.4 267,001 - EX31_RS01205 0.62 +0.2 267,163 + EX31_RS01205 0.80 +0.2 267,163 + EX31_RS01205 0.80 -0.8 267,167 - EX31_RS01205 0.80 +0.9 267,167 - EX31_RS01205 0.80 -0.5 267,172 - EX31_RS01205 0.81 -0.3 267,247 - EX31_RS01205 0.89 -0.5 267,247 - EX31_RS01205 0.89 +0.7 267,248 + EX31_RS01205 0.89 +0.3 267,248 + EX31_RS01205 0.89 +0.8 267,248 + EX31_RS01205 0.89 +1.4 267,248 + EX31_RS01205 0.89 -0.1 267,249 - EX31_RS01205 0.89 +0.9 267,249 - EX31_RS01205 0.89 +0.4 267,249 - EX31_RS01205 0.89 -0.7 267,249 - EX31_RS01205 0.89 +1.0 267,603 + -1.1 267,603 + -0.1 267,610 + -0.6 267,610 + -0.1 267,611 - -0.2 267,616 + -0.0 267,617 - +1.4 267,617 - -0.5 267,620 + -0.1 267,667 + -0.4 267,700 + -0.2 267,752 - -0.1 267,755 + -1.2 267,756 - +1.2 267,788 + +0.1 267,848 + -0.2 268,007 - EX31_RS01210 0.15 +1.4 268,007 - EX31_RS01210 0.15 +0.2 268,218 - EX31_RS01210 0.25 -1.9
Or see this region's nucleotide sequence