Strain Fitness in Rahnella sp. WP5 around EX31_RS00325

Experiment: Ammonium chloride (N); RCH2 media; no shaking

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntEX31_RS00315 and EX31_RS00320 are separated by 55 nucleotidesEX31_RS00320 and EX31_RS00325 are separated by 66 nucleotidesEX31_RS00325 and EX31_RS25025 are separated by 198 nucleotidesEX31_RS25025 and EX31_RS00330 are separated by 212 nucleotides EX31_RS00315: EX31_RS00315 - heme-binding protein, at 67,024 to 67,527 _RS00315 EX31_RS00320: EX31_RS00320 - HAD-IA family hydrolase, at 67,583 to 68,272 _RS00320 EX31_RS00325: EX31_RS00325 - hypothetical protein, at 68,339 to 68,983 _RS00325 EX31_RS25025: EX31_RS25025 - DUF1272 domain-containing protein, at 69,182 to 69,421 _RS25025 EX31_RS00330: EX31_RS00330 - drug:proton antiporter, at 69,634 to 70,005 _RS00330 Position (kb) 68 69Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 67.419 kb on + strand, within EX31_RS00315at 67.747 kb on + strand, within EX31_RS00320at 67.803 kb on + strand, within EX31_RS00320at 67.803 kb on + strand, within EX31_RS00320at 67.803 kb on + strand, within EX31_RS00320at 67.803 kb on + strand, within EX31_RS00320at 67.837 kb on + strand, within EX31_RS00320at 67.837 kb on + strand, within EX31_RS00320at 67.837 kb on + strand, within EX31_RS00320at 67.886 kb on + strand, within EX31_RS00320at 67.896 kb on + strand, within EX31_RS00320at 67.897 kb on - strand, within EX31_RS00320at 67.910 kb on - strand, within EX31_RS00320at 67.954 kb on + strand, within EX31_RS00320at 67.955 kb on - strand, within EX31_RS00320at 67.978 kb on + strand, within EX31_RS00320at 68.006 kb on + strand, within EX31_RS00320at 68.006 kb on + strand, within EX31_RS00320at 68.006 kb on + strand, within EX31_RS00320at 68.006 kb on + strand, within EX31_RS00320at 68.007 kb on - strand, within EX31_RS00320at 68.007 kb on - strand, within EX31_RS00320at 68.008 kb on + strand, within EX31_RS00320at 68.008 kb on + strand, within EX31_RS00320at 68.008 kb on + strand, within EX31_RS00320at 68.008 kb on + strand, within EX31_RS00320at 68.009 kb on - strand, within EX31_RS00320at 68.009 kb on - strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.019 kb on + strand, within EX31_RS00320at 68.020 kb on - strand, within EX31_RS00320at 68.020 kb on - strand, within EX31_RS00320at 68.020 kb on - strand, within EX31_RS00320at 68.020 kb on - strand, within EX31_RS00320at 68.067 kb on + strand, within EX31_RS00320at 68.079 kb on + strand, within EX31_RS00320at 68.080 kb on - strand, within EX31_RS00320at 68.107 kb on + strand, within EX31_RS00320at 68.108 kb on - strand, within EX31_RS00320at 68.112 kb on + strand, within EX31_RS00320at 68.128 kb on + strand, within EX31_RS00320at 68.129 kb on - strand, within EX31_RS00320at 68.129 kb on - strand, within EX31_RS00320at 68.129 kb on - strand, within EX31_RS00320at 68.242 kb on + strandat 68.330 kb on + strandat 68.330 kb on + strandat 68.330 kb on + strandat 68.330 kb on + strandat 68.331 kb on - strandat 68.331 kb on - strandat 68.331 kb on - strandat 68.331 kb on - strandat 68.368 kb on - strandat 68.447 kb on - strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.502 kb on + strand, within EX31_RS00325at 68.503 kb on - strand, within EX31_RS00325at 68.503 kb on - strand, within EX31_RS00325at 68.503 kb on - strand, within EX31_RS00325at 68.503 kb on - strand, within EX31_RS00325at 68.503 kb on - strand, within EX31_RS00325at 68.570 kb on - strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.748 kb on + strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.749 kb on - strand, within EX31_RS00325at 68.778 kb on + strand, within EX31_RS00325at 68.779 kb on - strand, within EX31_RS00325at 68.781 kb on + strand, within EX31_RS00325at 68.781 kb on + strand, within EX31_RS00325at 68.782 kb on - strand, within EX31_RS00325at 68.782 kb on - strand, within EX31_RS00325at 68.782 kb on - strand, within EX31_RS00325at 68.782 kb on - strand, within EX31_RS00325at 68.804 kb on - strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.867 kb on + strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.868 kb on - strand, within EX31_RS00325at 68.897 kb on + strand, within EX31_RS00325at 68.898 kb on - strand, within EX31_RS00325at 68.997 kb on - strandat 68.997 kb on - strandat 69.029 kb on + strandat 69.063 kb on + strandat 69.064 kb on - strandat 69.064 kb on - strandat 69.091 kb on + strandat 69.091 kb on + strandat 69.091 kb on + strandat 69.091 kb on + strandat 69.092 kb on - strandat 69.092 kb on - strandat 69.092 kb on - strandat 69.093 kb on + strandat 69.094 kb on - strandat 69.094 kb on - strandat 69.094 kb on - strandat 69.094 kb on - strandat 69.094 kb on - strandat 69.106 kb on - strandat 69.106 kb on - strandat 69.184 kb on - strandat 69.184 kb on - strandat 69.184 kb on - strandat 69.206 kb on - strand, within EX31_RS25025at 69.210 kb on - strand, within EX31_RS25025at 69.210 kb on - strand, within EX31_RS25025at 69.219 kb on - strand, within EX31_RS25025at 69.296 kb on + strand, within EX31_RS25025at 69.421 kb on + strandat 69.421 kb on + strandat 69.421 kb on + strandat 69.421 kb on + strandat 69.442 kb on - strandat 69.489 kb on - strandat 69.489 kb on - strandat 69.494 kb on + strandat 69.495 kb on - strandat 69.654 kb on - strandat 69.697 kb on - strand, within EX31_RS00330at 69.699 kb on - strand, within EX31_RS00330at 69.731 kb on - strand, within EX31_RS00330at 69.731 kb on - strand, within EX31_RS00330at 69.822 kb on - strand, within EX31_RS00330at 69.847 kb on - strand, within EX31_RS00330at 69.847 kb on - strand, within EX31_RS00330at 69.945 kb on - strand, within EX31_RS00330at 69.977 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking
remove
67,419 + EX31_RS00315 0.78 -0.5
67,747 + EX31_RS00320 0.24 +0.3
67,803 + EX31_RS00320 0.32 -0.2
67,803 + EX31_RS00320 0.32 +0.8
67,803 + EX31_RS00320 0.32 -0.5
67,803 + EX31_RS00320 0.32 +1.1
67,837 + EX31_RS00320 0.37 +1.7
67,837 + EX31_RS00320 0.37 +1.0
67,837 + EX31_RS00320 0.37 +1.1
67,886 + EX31_RS00320 0.44 +0.4
67,896 + EX31_RS00320 0.45 +0.9
67,897 - EX31_RS00320 0.46 +0.0
67,910 - EX31_RS00320 0.47 -0.6
67,954 + EX31_RS00320 0.54 +2.1
67,955 - EX31_RS00320 0.54 +1.1
67,978 + EX31_RS00320 0.57 +0.9
68,006 + EX31_RS00320 0.61 +0.3
68,006 + EX31_RS00320 0.61 -1.2
68,006 + EX31_RS00320 0.61 -0.7
68,006 + EX31_RS00320 0.61 +1.4
68,007 - EX31_RS00320 0.61 -0.7
68,007 - EX31_RS00320 0.61 +0.9
68,008 + EX31_RS00320 0.62 -0.5
68,008 + EX31_RS00320 0.62 -0.2
68,008 + EX31_RS00320 0.62 +0.3
68,008 + EX31_RS00320 0.62 +2.1
68,009 - EX31_RS00320 0.62 +1.1
68,009 - EX31_RS00320 0.62 +0.5
68,019 + EX31_RS00320 0.63 +1.2
68,019 + EX31_RS00320 0.63 +1.1
68,019 + EX31_RS00320 0.63 -0.5
68,019 + EX31_RS00320 0.63 -0.0
68,019 + EX31_RS00320 0.63 -0.6
68,019 + EX31_RS00320 0.63 -2.4
68,019 + EX31_RS00320 0.63 +0.9
68,019 + EX31_RS00320 0.63 +1.4
68,019 + EX31_RS00320 0.63 -1.3
68,019 + EX31_RS00320 0.63 -0.2
68,020 - EX31_RS00320 0.63 +0.4
68,020 - EX31_RS00320 0.63 +0.4
68,020 - EX31_RS00320 0.63 +1.1
68,020 - EX31_RS00320 0.63 -0.4
68,067 + EX31_RS00320 0.70 -1.9
68,079 + EX31_RS00320 0.72 +0.3
68,080 - EX31_RS00320 0.72 -0.9
68,107 + EX31_RS00320 0.76 -0.3
68,108 - EX31_RS00320 0.76 +1.3
68,112 + EX31_RS00320 0.77 +0.8
68,128 + EX31_RS00320 0.79 +0.3
68,129 - EX31_RS00320 0.79 -1.4
68,129 - EX31_RS00320 0.79 +1.4
68,129 - EX31_RS00320 0.79 -1.7
68,242 + -0.5
68,330 + +0.5
68,330 + -0.3
68,330 + -0.4
68,330 + +0.6
68,331 - -0.4
68,331 - -0.0
68,331 - +1.1
68,331 - +1.1
68,368 - -0.1
68,447 - EX31_RS00325 0.17 +1.1
68,502 + EX31_RS00325 0.25 -1.2
68,502 + EX31_RS00325 0.25 +0.8
68,502 + EX31_RS00325 0.25 -1.0
68,502 + EX31_RS00325 0.25 -0.8
68,502 + EX31_RS00325 0.25 +1.8
68,502 + EX31_RS00325 0.25 -0.5
68,502 + EX31_RS00325 0.25 -0.3
68,503 - EX31_RS00325 0.25 +3.1
68,503 - EX31_RS00325 0.25 +0.2
68,503 - EX31_RS00325 0.25 +0.5
68,503 - EX31_RS00325 0.25 +1.4
68,503 - EX31_RS00325 0.25 -1.3
68,570 - EX31_RS00325 0.36 -0.7
68,748 + EX31_RS00325 0.63 +0.8
68,748 + EX31_RS00325 0.63 -0.9
68,748 + EX31_RS00325 0.63 -2.2
68,748 + EX31_RS00325 0.63 -1.0
68,748 + EX31_RS00325 0.63 -0.9
68,748 + EX31_RS00325 0.63 +0.5
68,748 + EX31_RS00325 0.63 -2.3
68,749 - EX31_RS00325 0.64 -1.0
68,749 - EX31_RS00325 0.64 +0.1
68,749 - EX31_RS00325 0.64 +0.1
68,749 - EX31_RS00325 0.64 -2.3
68,749 - EX31_RS00325 0.64 +1.2
68,749 - EX31_RS00325 0.64 -1.1
68,749 - EX31_RS00325 0.64 +0.1
68,749 - EX31_RS00325 0.64 -1.4
68,749 - EX31_RS00325 0.64 -0.1
68,778 + EX31_RS00325 0.68 -0.5
68,779 - EX31_RS00325 0.68 +0.1
68,781 + EX31_RS00325 0.69 +0.3
68,781 + EX31_RS00325 0.69 -0.9
68,782 - EX31_RS00325 0.69 -0.6
68,782 - EX31_RS00325 0.69 -0.1
68,782 - EX31_RS00325 0.69 +0.8
68,782 - EX31_RS00325 0.69 +0.5
68,804 - EX31_RS00325 0.72 -0.6
68,867 + EX31_RS00325 0.82 -2.0
68,867 + EX31_RS00325 0.82 +0.6
68,867 + EX31_RS00325 0.82 -0.2
68,867 + EX31_RS00325 0.82 -0.1
68,867 + EX31_RS00325 0.82 +1.1
68,867 + EX31_RS00325 0.82 -0.7
68,867 + EX31_RS00325 0.82 +0.9
68,867 + EX31_RS00325 0.82 -0.2
68,867 + EX31_RS00325 0.82 -0.5
68,867 + EX31_RS00325 0.82 +1.1
68,868 - EX31_RS00325 0.82 -2.4
68,868 - EX31_RS00325 0.82 +0.5
68,868 - EX31_RS00325 0.82 -0.6
68,868 - EX31_RS00325 0.82 -0.3
68,868 - EX31_RS00325 0.82 -0.1
68,868 - EX31_RS00325 0.82 -0.9
68,868 - EX31_RS00325 0.82 -2.4
68,897 + EX31_RS00325 0.87 +1.2
68,898 - EX31_RS00325 0.87 -1.1
68,997 - -0.0
68,997 - -1.0
69,029 + -0.3
69,063 + -0.2
69,064 - -0.2
69,064 - -0.5
69,091 + +0.2
69,091 + +0.4
69,091 + +0.7
69,091 + +1.5
69,092 - +0.9
69,092 - +0.1
69,092 - -0.7
69,093 + -1.7
69,094 - -0.2
69,094 - +0.5
69,094 - +0.5
69,094 - +0.6
69,094 - +0.8
69,106 - +0.0
69,106 - +0.8
69,184 - -2.8
69,184 - +0.2
69,184 - -0.9
69,206 - EX31_RS25025 0.10 -1.2
69,210 - EX31_RS25025 0.12 +1.8
69,210 - EX31_RS25025 0.12 -0.9
69,219 - EX31_RS25025 0.15 -0.2
69,296 + EX31_RS25025 0.47 +1.1
69,421 + +0.1
69,421 + -0.5
69,421 + -0.5
69,421 + +0.4
69,442 - -0.9
69,489 - -0.4
69,489 - -0.2
69,494 + -0.7
69,495 - -0.1
69,654 - +0.1
69,697 - EX31_RS00330 0.17 +0.7
69,699 - EX31_RS00330 0.17 +1.3
69,731 - EX31_RS00330 0.26 -0.9
69,731 - EX31_RS00330 0.26 +0.5
69,822 - EX31_RS00330 0.51 -1.3
69,847 - EX31_RS00330 0.57 +0.7
69,847 - EX31_RS00330 0.57 +0.3
69,945 - EX31_RS00330 0.84 +0.9
69,977 + +0.5

Or see this region's nucleotide sequence