Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1858 and BT1859 are separated by 5 nucleotides BT1859 and BT1860 are separated by 42 nucleotides BT1860 and BT1861 are separated by 77 nucleotides
BT1858: BT1858 - (R)-citramalate synthase (EC 2.3.1.182) (from data) , at 2,331,081 to 2,332,580
BT1858
BT1859: BT1859 - 3-isopropylmalate dehydratase small subunit (NCBI ptt file), at 2,332,586 to 2,333,188
BT1859
BT1860: BT1860 - 3-isopropylmalate dehydratase large subunit (NCBI ptt file), at 2,333,231 to 2,334,625
BT1860
BT1861: BT1861 - 2-isopropylmalate synthase (EC 2.3.3.13) (from data) , at 2,334,703 to 2,336,199
BT1861
Position (kb)
2333
2334
2335 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2332.232 kb on + strand, within BT1858 at 2332.296 kb on - strand, within BT1858 at 2332.332 kb on + strand, within BT1858 at 2332.350 kb on - strand, within BT1858 at 2332.588 kb on - strand at 2332.588 kb on - strand at 2332.646 kb on + strand at 2332.724 kb on - strand, within BT1859 at 2332.833 kb on + strand, within BT1859 at 2332.884 kb on - strand, within BT1859 at 2332.884 kb on - strand, within BT1859 at 2332.983 kb on + strand, within BT1859 at 2332.984 kb on - strand, within BT1859 at 2333.032 kb on + strand, within BT1859 at 2333.056 kb on + strand, within BT1859 at 2333.056 kb on + strand, within BT1859 at 2333.110 kb on + strand, within BT1859 at 2333.122 kb on + strand, within BT1859 at 2333.126 kb on + strand, within BT1859 at 2333.127 kb on - strand, within BT1859 at 2333.156 kb on - strand at 2333.163 kb on - strand at 2333.206 kb on - strand at 2333.214 kb on - strand at 2333.308 kb on + strand at 2333.309 kb on - strand at 2333.590 kb on + strand, within BT1860 at 2333.610 kb on + strand, within BT1860 at 2333.610 kb on + strand, within BT1860 at 2333.611 kb on - strand, within BT1860 at 2333.659 kb on - strand, within BT1860 at 2333.711 kb on - strand, within BT1860 at 2333.714 kb on - strand, within BT1860 at 2333.765 kb on - strand, within BT1860 at 2333.936 kb on - strand, within BT1860 at 2333.968 kb on + strand, within BT1860 at 2333.978 kb on - strand, within BT1860 at 2334.021 kb on + strand, within BT1860 at 2334.124 kb on + strand, within BT1860 at 2334.158 kb on - strand, within BT1860 at 2334.376 kb on + strand, within BT1860 at 2334.436 kb on + strand, within BT1860 at 2334.551 kb on - strand at 2334.552 kb on + strand at 2334.553 kb on - strand at 2334.630 kb on + strand at 2334.631 kb on - strand at 2334.631 kb on - strand at 2334.646 kb on - strand at 2334.650 kb on - strand at 2334.673 kb on - strand at 2334.734 kb on + strand at 2334.735 kb on - strand at 2334.742 kb on + strand at 2334.756 kb on + strand at 2334.765 kb on + strand at 2334.765 kb on + strand at 2334.765 kb on + strand at 2334.813 kb on + strand at 2334.814 kb on - strand at 2334.823 kb on - strand at 2334.934 kb on - strand, within BT1861 at 2334.969 kb on + strand, within BT1861 at 2335.038 kb on + strand, within BT1861 at 2335.039 kb on - strand, within BT1861 at 2335.073 kb on - strand, within BT1861 at 2335.078 kb on + strand, within BT1861 at 2335.078 kb on + strand, within BT1861 at 2335.096 kb on - strand, within BT1861 at 2335.135 kb on + strand, within BT1861 at 2335.136 kb on - strand, within BT1861 at 2335.136 kb on - strand, within BT1861 at 2335.246 kb on + strand, within BT1861 at 2335.246 kb on + strand, within BT1861 at 2335.247 kb on - strand, within BT1861 at 2335.280 kb on - strand, within BT1861 at 2335.368 kb on + strand, within BT1861 at 2335.368 kb on + strand, within BT1861 at 2335.561 kb on - strand, within BT1861 at 2335.588 kb on - strand, within BT1861
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 2,332,232 + BT1858 0.77 -3.4 2,332,296 - BT1858 0.81 -3.8 2,332,332 + BT1858 0.83 -1.4 2,332,350 - BT1858 0.85 -2.1 2,332,588 - -3.9 2,332,588 - -0.6 2,332,646 + -2.1 2,332,724 - BT1859 0.23 -3.0 2,332,833 + BT1859 0.41 -4.8 2,332,884 - BT1859 0.49 -3.3 2,332,884 - BT1859 0.49 -2.1 2,332,983 + BT1859 0.66 -2.4 2,332,984 - BT1859 0.66 -2.1 2,333,032 + BT1859 0.74 -1.6 2,333,056 + BT1859 0.78 -2.6 2,333,056 + BT1859 0.78 -2.5 2,333,110 + BT1859 0.87 -1.4 2,333,122 + BT1859 0.89 -1.6 2,333,126 + BT1859 0.90 -2.5 2,333,127 - BT1859 0.90 -3.2 2,333,156 - -2.1 2,333,163 - -2.5 2,333,206 - -1.6 2,333,214 - -2.2 2,333,308 + -0.5 2,333,309 - -3.1 2,333,590 + BT1860 0.26 -1.6 2,333,610 + BT1860 0.27 -1.5 2,333,610 + BT1860 0.27 -1.9 2,333,611 - BT1860 0.27 -3.5 2,333,659 - BT1860 0.31 -2.8 2,333,711 - BT1860 0.34 -1.1 2,333,714 - BT1860 0.35 +0.0 2,333,765 - BT1860 0.38 -2.2 2,333,936 - BT1860 0.51 -1.6 2,333,968 + BT1860 0.53 -0.5 2,333,978 - BT1860 0.54 -1.9 2,334,021 + BT1860 0.57 -3.2 2,334,124 + BT1860 0.64 -1.7 2,334,158 - BT1860 0.66 -3.7 2,334,376 + BT1860 0.82 -3.1 2,334,436 + BT1860 0.86 -2.1 2,334,551 - -3.3 2,334,552 + -2.5 2,334,553 - -2.9 2,334,630 + -3.7 2,334,631 - -3.3 2,334,631 - -3.4 2,334,646 - -0.4 2,334,650 - -0.5 2,334,673 - -1.7 2,334,734 + -1.4 2,334,735 - -2.0 2,334,742 + -2.4 2,334,756 + -2.6 2,334,765 + -3.5 2,334,765 + -3.4 2,334,765 + -3.1 2,334,813 + -3.8 2,334,814 - -1.4 2,334,823 - -2.5 2,334,934 - BT1861 0.15 -1.5 2,334,969 + BT1861 0.18 +0.2 2,335,038 + BT1861 0.22 -1.5 2,335,039 - BT1861 0.22 -0.9 2,335,073 - BT1861 0.25 -1.5 2,335,078 + BT1861 0.25 -1.1 2,335,078 + BT1861 0.25 -1.1 2,335,096 - BT1861 0.26 -3.0 2,335,135 + BT1861 0.29 -2.7 2,335,136 - BT1861 0.29 -1.7 2,335,136 - BT1861 0.29 -2.8 2,335,246 + BT1861 0.36 -1.9 2,335,246 + BT1861 0.36 -3.4 2,335,247 - BT1861 0.36 -2.1 2,335,280 - BT1861 0.39 -3.6 2,335,368 + BT1861 0.44 -2.6 2,335,368 + BT1861 0.44 -2.3 2,335,561 - BT1861 0.57 +1.1 2,335,588 - BT1861 0.59 -1.1
Or see this region's nucleotide sequence