Strain Fitness in Rahnella sp. WP5 around EX31_RS23440

Experiment: Ammonium chloride (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS23435 and EX31_RS23440 are separated by 400 nucleotidesEX31_RS23440 and EX31_RS23445 are separated by 46 nucleotides EX31_RS23435: EX31_RS23435 - succinate dehydrogenase iron-sulfur subunit, at 453,259 to 453,975 _RS23435 EX31_RS23440: EX31_RS23440 - 2-oxoglutarate dehydrogenase E1 component, at 454,376 to 457,183 _RS23440 EX31_RS23445: EX31_RS23445 - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 457,230 to 458,459 _RS23445 Position (kb) 454 455 456 457 458Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 453.483 kb on + strand, within EX31_RS23435at 453.483 kb on + strand, within EX31_RS23435at 453.483 kb on + strand, within EX31_RS23435at 453.570 kb on - strand, within EX31_RS23435at 453.572 kb on + strand, within EX31_RS23435at 453.572 kb on + strand, within EX31_RS23435at 453.572 kb on + strand, within EX31_RS23435at 453.589 kb on + strand, within EX31_RS23435at 453.609 kb on + strand, within EX31_RS23435at 453.616 kb on + strand, within EX31_RS23435at 453.616 kb on + strand, within EX31_RS23435at 453.616 kb on + strand, within EX31_RS23435at 453.616 kb on + strand, within EX31_RS23435at 453.697 kb on + strand, within EX31_RS23435at 453.697 kb on + strand, within EX31_RS23435at 453.705 kb on + strand, within EX31_RS23435at 453.705 kb on + strand, within EX31_RS23435at 453.705 kb on + strand, within EX31_RS23435at 453.706 kb on - strand, within EX31_RS23435at 453.902 kb on + strand, within EX31_RS23435at 453.903 kb on - strand, within EX31_RS23435at 453.945 kb on + strandat 453.945 kb on + strandat 453.946 kb on - strandat 453.946 kb on - strandat 453.959 kb on + strandat 453.959 kb on + strandat 453.980 kb on + strandat 453.980 kb on + strandat 454.020 kb on + strandat 454.020 kb on + strandat 454.021 kb on - strandat 454.021 kb on - strandat 454.048 kb on - strandat 454.062 kb on + strandat 454.062 kb on + strandat 454.143 kb on + strandat 454.207 kb on + strandat 454.207 kb on + strandat 454.207 kb on + strandat 454.305 kb on + strandat 454.305 kb on + strandat 454.315 kb on + strandat 454.315 kb on + strandat 454.327 kb on + strandat 454.327 kb on + strandat 454.327 kb on + strandat 454.330 kb on + strandat 454.330 kb on + strandat 454.349 kb on + strandat 454.463 kb on + strandat 454.889 kb on + strand, within EX31_RS23440at 454.900 kb on + strand, within EX31_RS23440at 454.900 kb on + strand, within EX31_RS23440at 454.993 kb on + strand, within EX31_RS23440at 455.536 kb on + strand, within EX31_RS23440at 455.545 kb on + strand, within EX31_RS23440at 455.605 kb on + strand, within EX31_RS23440at 455.779 kb on + strand, within EX31_RS23440at 455.801 kb on + strand, within EX31_RS23440at 455.937 kb on + strand, within EX31_RS23440at 455.987 kb on + strand, within EX31_RS23440at 456.313 kb on + strand, within EX31_RS23440at 456.653 kb on + strand, within EX31_RS23440at 457.077 kb on + strandat 457.204 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking
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453,483 + EX31_RS23435 0.31 -0.3
453,483 + EX31_RS23435 0.31 +0.6
453,483 + EX31_RS23435 0.31 +0.5
453,570 - EX31_RS23435 0.43 +1.4
453,572 + EX31_RS23435 0.44 -1.5
453,572 + EX31_RS23435 0.44 +0.0
453,572 + EX31_RS23435 0.44 -0.5
453,589 + EX31_RS23435 0.46 +0.0
453,609 + EX31_RS23435 0.49 -0.8
453,616 + EX31_RS23435 0.50 +2.5
453,616 + EX31_RS23435 0.50 -3.0
453,616 + EX31_RS23435 0.50 -0.5
453,616 + EX31_RS23435 0.50 -0.8
453,697 + EX31_RS23435 0.61 +0.3
453,697 + EX31_RS23435 0.61 +0.7
453,705 + EX31_RS23435 0.62 +1.3
453,705 + EX31_RS23435 0.62 +0.4
453,705 + EX31_RS23435 0.62 -0.4
453,706 - EX31_RS23435 0.62 -0.1
453,902 + EX31_RS23435 0.90 +1.4
453,903 - EX31_RS23435 0.90 +1.0
453,945 + +0.6
453,945 + -0.7
453,946 - +2.0
453,946 - +1.6
453,959 + +0.0
453,959 + -0.1
453,980 + +0.6
453,980 + -0.4
454,020 + -0.5
454,020 + +0.2
454,021 - +0.3
454,021 - +1.6
454,048 - -1.0
454,062 + +0.2
454,062 + +0.2
454,143 + +0.3
454,207 + +1.0
454,207 + +1.2
454,207 + +0.5
454,305 + +1.0
454,305 + -0.3
454,315 + -0.3
454,315 + +0.7
454,327 + +0.2
454,327 + +0.7
454,327 + -0.1
454,330 + +0.7
454,330 + -0.8
454,349 + +0.2
454,463 + -0.3
454,889 + EX31_RS23440 0.18 -0.5
454,900 + EX31_RS23440 0.19 -0.5
454,900 + EX31_RS23440 0.19 -0.3
454,993 + EX31_RS23440 0.22 -1.1
455,536 + EX31_RS23440 0.41 -2.2
455,545 + EX31_RS23440 0.42 -2.0
455,605 + EX31_RS23440 0.44 +0.5
455,779 + EX31_RS23440 0.50 +2.0
455,801 + EX31_RS23440 0.51 -0.5
455,937 + EX31_RS23440 0.56 -0.8
455,987 + EX31_RS23440 0.57 -1.3
456,313 + EX31_RS23440 0.69 -1.9
456,653 + EX31_RS23440 0.81 -0.8
457,077 + -2.0
457,204 + +0.2

Or see this region's nucleotide sequence