Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21080 and EX31_RS21085 are separated by 13 nucleotides EX31_RS21085 and EX31_RS21090 overlap by 1 nucleotides EX31_RS21090 and EX31_RS21095 overlap by 4 nucleotides
EX31_RS21080: EX31_RS21080 - glutamine amidotransferase, at 860,568 to 861,836
_RS21080
EX31_RS21085: EX31_RS21085 - hydroxyisourate hydrolase, at 861,850 to 862,185
_RS21085
EX31_RS21090: EX31_RS21090 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, at 862,185 to 862,679
_RS21090
EX31_RS21095: EX31_RS21095 - allantoate amidohydrolase, at 862,676 to 863,950
_RS21095
Position (kb) 861
862
863 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 861.017 kb on - strand, within EX31_RS21080 at 861.084 kb on - strand, within EX31_RS21080 at 861.103 kb on - strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.168 kb on + strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.169 kb on - strand, within EX31_RS21080 at 861.230 kb on + strand, within EX31_RS21080 at 861.230 kb on + strand, within EX31_RS21080 at 861.230 kb on + strand, within EX31_RS21080 at 861.230 kb on + strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.231 kb on - strand, within EX31_RS21080 at 861.322 kb on + strand, within EX31_RS21080 at 861.322 kb on + strand, within EX31_RS21080 at 861.323 kb on - strand, within EX31_RS21080 at 861.323 kb on - strand, within EX31_RS21080 at 861.329 kb on + strand, within EX31_RS21080 at 861.330 kb on - strand, within EX31_RS21080 at 861.338 kb on + strand, within EX31_RS21080 at 861.338 kb on + strand, within EX31_RS21080 at 861.339 kb on - strand, within EX31_RS21080 at 861.339 kb on - strand, within EX31_RS21080 at 861.530 kb on + strand, within EX31_RS21080 at 861.530 kb on + strand, within EX31_RS21080 at 861.530 kb on + strand, within EX31_RS21080 at 861.531 kb on - strand, within EX31_RS21080 at 861.531 kb on - strand, within EX31_RS21080 at 861.531 kb on - strand, within EX31_RS21080 at 861.540 kb on + strand, within EX31_RS21080 at 861.541 kb on - strand, within EX31_RS21080 at 861.809 kb on + strand at 861.810 kb on - strand at 861.810 kb on - strand at 861.851 kb on + strand at 861.852 kb on - strand at 861.852 kb on - strand at 861.852 kb on - strand at 861.852 kb on - strand at 861.863 kb on + strand at 861.864 kb on - strand at 861.873 kb on - strand at 861.873 kb on - strand at 861.873 kb on - strand at 861.878 kb on + strand at 861.879 kb on - strand at 861.905 kb on + strand, within EX31_RS21085 at 861.905 kb on + strand, within EX31_RS21085 at 861.905 kb on + strand, within EX31_RS21085 at 861.983 kb on + strand, within EX31_RS21085 at 861.983 kb on + strand, within EX31_RS21085 at 861.983 kb on + strand, within EX31_RS21085 at 861.983 kb on + strand, within EX31_RS21085 at 861.983 kb on + strand, within EX31_RS21085 at 861.983 kb on + strand, within EX31_RS21085 at 861.984 kb on - strand, within EX31_RS21085 at 861.984 kb on - strand, within EX31_RS21085 at 861.984 kb on - strand, within EX31_RS21085 at 861.984 kb on - strand, within EX31_RS21085 at 862.107 kb on + strand, within EX31_RS21085 at 862.107 kb on + strand, within EX31_RS21085 at 862.107 kb on + strand, within EX31_RS21085 at 862.107 kb on + strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.108 kb on - strand, within EX31_RS21085 at 862.155 kb on + strand at 862.155 kb on + strand at 862.164 kb on + strand at 862.164 kb on + strand at 862.171 kb on - strand at 862.195 kb on - strand at 862.195 kb on - strand at 862.195 kb on - strand at 862.217 kb on + strand at 862.289 kb on + strand, within EX31_RS21090 at 862.290 kb on - strand, within EX31_RS21090 at 862.367 kb on + strand, within EX31_RS21090 at 862.367 kb on + strand, within EX31_RS21090 at 862.367 kb on + strand, within EX31_RS21090 at 862.448 kb on + strand, within EX31_RS21090 at 862.506 kb on - strand, within EX31_RS21090 at 862.506 kb on - strand, within EX31_RS21090 at 862.551 kb on + strand, within EX31_RS21090 at 862.552 kb on - strand, within EX31_RS21090 at 862.552 kb on - strand, within EX31_RS21090 at 862.552 kb on - strand, within EX31_RS21090 at 862.552 kb on - strand, within EX31_RS21090 at 862.570 kb on + strand, within EX31_RS21090 at 862.570 kb on + strand, within EX31_RS21090 at 862.571 kb on - strand, within EX31_RS21090 at 862.571 kb on - strand, within EX31_RS21090 at 862.571 kb on - strand, within EX31_RS21090 at 862.610 kb on + strand, within EX31_RS21090 at 862.611 kb on - strand, within EX31_RS21090 at 862.626 kb on + strand, within EX31_RS21090 at 862.627 kb on - strand, within EX31_RS21090 at 862.679 kb on + strand at 862.679 kb on + strand at 862.680 kb on - strand at 862.680 kb on - strand at 862.680 kb on - strand at 862.680 kb on - strand at 862.680 kb on - strand at 862.708 kb on + strand at 862.714 kb on + strand at 862.714 kb on + strand at 862.714 kb on + strand at 862.715 kb on - strand at 862.715 kb on - strand at 862.715 kb on - strand at 862.715 kb on - strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.716 kb on + strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.717 kb on - strand at 862.732 kb on - strand at 862.786 kb on + strand at 862.787 kb on - strand at 862.828 kb on + strand, within EX31_RS21095 at 862.887 kb on - strand, within EX31_RS21095 at 862.887 kb on - strand, within EX31_RS21095 at 862.924 kb on + strand, within EX31_RS21095 at 862.924 kb on + strand, within EX31_RS21095 at 862.924 kb on + strand, within EX31_RS21095 at 862.924 kb on + strand, within EX31_RS21095 at 862.925 kb on - strand, within EX31_RS21095 at 862.947 kb on + strand, within EX31_RS21095 at 862.947 kb on + strand, within EX31_RS21095 at 862.947 kb on + strand, within EX31_RS21095 at 862.947 kb on + strand, within EX31_RS21095 at 862.948 kb on - strand, within EX31_RS21095 at 862.948 kb on - strand, within EX31_RS21095 at 863.031 kb on - strand, within EX31_RS21095 at 863.055 kb on - strand, within EX31_RS21095 at 863.055 kb on - strand, within EX31_RS21095 at 863.161 kb on + strand, within EX31_RS21095 at 863.161 kb on + strand, within EX31_RS21095
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 861,017 - EX31_RS21080 0.35 -0.2 861,084 - EX31_RS21080 0.41 -1.8 861,103 - EX31_RS21080 0.42 +1.0 861,168 + EX31_RS21080 0.47 +0.3 861,168 + EX31_RS21080 0.47 +0.3 861,168 + EX31_RS21080 0.47 -0.6 861,168 + EX31_RS21080 0.47 +2.0 861,168 + EX31_RS21080 0.47 +0.1 861,168 + EX31_RS21080 0.47 -0.8 861,168 + EX31_RS21080 0.47 -0.6 861,168 + EX31_RS21080 0.47 +1.9 861,168 + EX31_RS21080 0.47 +0.4 861,168 + EX31_RS21080 0.47 +0.2 861,168 + EX31_RS21080 0.47 -1.4 861,168 + EX31_RS21080 0.47 -0.4 861,168 + EX31_RS21080 0.47 +0.3 861,168 + EX31_RS21080 0.47 +0.8 861,168 + EX31_RS21080 0.47 -0.3 861,168 + EX31_RS21080 0.47 -0.4 861,168 + EX31_RS21080 0.47 +0.4 861,168 + EX31_RS21080 0.47 +0.8 861,168 + EX31_RS21080 0.47 -0.3 861,168 + EX31_RS21080 0.47 -0.0 861,168 + EX31_RS21080 0.47 +0.9 861,168 + EX31_RS21080 0.47 +2.2 861,168 + EX31_RS21080 0.47 +1.0 861,168 + EX31_RS21080 0.47 +0.1 861,168 + EX31_RS21080 0.47 +0.5 861,169 - EX31_RS21080 0.47 +0.7 861,169 - EX31_RS21080 0.47 +0.1 861,169 - EX31_RS21080 0.47 +0.5 861,169 - EX31_RS21080 0.47 +1.4 861,169 - EX31_RS21080 0.47 +0.2 861,169 - EX31_RS21080 0.47 -0.0 861,169 - EX31_RS21080 0.47 +0.2 861,169 - EX31_RS21080 0.47 -1.0 861,169 - EX31_RS21080 0.47 +1.2 861,169 - EX31_RS21080 0.47 -0.2 861,169 - EX31_RS21080 0.47 +0.1 861,169 - EX31_RS21080 0.47 +1.0 861,230 + EX31_RS21080 0.52 -0.5 861,230 + EX31_RS21080 0.52 +0.4 861,230 + EX31_RS21080 0.52 -0.8 861,230 + EX31_RS21080 0.52 -0.3 861,231 - EX31_RS21080 0.52 -1.3 861,231 - EX31_RS21080 0.52 -0.0 861,231 - EX31_RS21080 0.52 +0.4 861,231 - EX31_RS21080 0.52 +0.7 861,231 - EX31_RS21080 0.52 -0.9 861,231 - EX31_RS21080 0.52 +1.6 861,231 - EX31_RS21080 0.52 +0.9 861,322 + EX31_RS21080 0.59 -1.1 861,322 + EX31_RS21080 0.59 +0.5 861,323 - EX31_RS21080 0.59 -0.9 861,323 - EX31_RS21080 0.59 +0.5 861,329 + EX31_RS21080 0.60 -0.4 861,330 - EX31_RS21080 0.60 -0.4 861,338 + EX31_RS21080 0.61 -0.5 861,338 + EX31_RS21080 0.61 +0.2 861,339 - EX31_RS21080 0.61 -1.4 861,339 - EX31_RS21080 0.61 +1.2 861,530 + EX31_RS21080 0.76 +0.5 861,530 + EX31_RS21080 0.76 +0.8 861,530 + EX31_RS21080 0.76 +0.4 861,531 - EX31_RS21080 0.76 +0.2 861,531 - EX31_RS21080 0.76 -0.1 861,531 - EX31_RS21080 0.76 -0.9 861,540 + EX31_RS21080 0.77 -0.2 861,541 - EX31_RS21080 0.77 +0.1 861,809 + -0.6 861,810 - -1.4 861,810 - -0.3 861,851 + -1.3 861,852 - -0.3 861,852 - -0.1 861,852 - +0.5 861,852 - +0.4 861,863 + +0.5 861,864 - +0.5 861,873 - +0.6 861,873 - +0.7 861,873 - -0.3 861,878 + -0.4 861,879 - +0.1 861,905 + EX31_RS21085 0.16 -1.3 861,905 + EX31_RS21085 0.16 +0.1 861,905 + EX31_RS21085 0.16 +0.5 861,983 + EX31_RS21085 0.40 -1.1 861,983 + EX31_RS21085 0.40 -1.2 861,983 + EX31_RS21085 0.40 +0.7 861,983 + EX31_RS21085 0.40 +3.2 861,983 + EX31_RS21085 0.40 +0.1 861,983 + EX31_RS21085 0.40 -0.3 861,984 - EX31_RS21085 0.40 +0.5 861,984 - EX31_RS21085 0.40 +0.2 861,984 - EX31_RS21085 0.40 +1.9 861,984 - EX31_RS21085 0.40 -0.0 862,107 + EX31_RS21085 0.76 +0.6 862,107 + EX31_RS21085 0.76 +0.1 862,107 + EX31_RS21085 0.76 -1.6 862,107 + EX31_RS21085 0.76 -1.0 862,108 - EX31_RS21085 0.77 -0.1 862,108 - EX31_RS21085 0.77 -1.8 862,108 - EX31_RS21085 0.77 -0.0 862,108 - EX31_RS21085 0.77 -1.1 862,108 - EX31_RS21085 0.77 +0.2 862,108 - EX31_RS21085 0.77 -0.8 862,108 - EX31_RS21085 0.77 -0.4 862,108 - EX31_RS21085 0.77 -0.1 862,155 + -0.1 862,155 + +0.8 862,164 + -0.4 862,164 + +2.2 862,171 - -1.1 862,195 - +0.5 862,195 - +0.2 862,195 - +0.5 862,217 + +0.8 862,289 + EX31_RS21090 0.21 -0.6 862,290 - EX31_RS21090 0.21 -0.8 862,367 + EX31_RS21090 0.37 -1.0 862,367 + EX31_RS21090 0.37 +0.8 862,367 + EX31_RS21090 0.37 -0.4 862,448 + EX31_RS21090 0.53 +0.1 862,506 - EX31_RS21090 0.65 -0.2 862,506 - EX31_RS21090 0.65 +0.4 862,551 + EX31_RS21090 0.74 -0.6 862,552 - EX31_RS21090 0.74 +1.5 862,552 - EX31_RS21090 0.74 +3.2 862,552 - EX31_RS21090 0.74 -0.5 862,552 - EX31_RS21090 0.74 -1.6 862,570 + EX31_RS21090 0.78 +0.9 862,570 + EX31_RS21090 0.78 -0.4 862,571 - EX31_RS21090 0.78 -1.6 862,571 - EX31_RS21090 0.78 +0.0 862,571 - EX31_RS21090 0.78 -0.6 862,610 + EX31_RS21090 0.86 +0.0 862,611 - EX31_RS21090 0.86 -1.4 862,626 + EX31_RS21090 0.89 -0.6 862,627 - EX31_RS21090 0.89 +0.5 862,679 + -0.3 862,679 + +1.5 862,680 - +0.7 862,680 - -0.1 862,680 - +1.3 862,680 - -0.5 862,680 - -1.0 862,708 + +0.3 862,714 + +0.1 862,714 + -0.4 862,714 + -0.8 862,715 - +0.4 862,715 - -0.1 862,715 - +0.3 862,715 - -0.2 862,716 + +0.6 862,716 + -0.5 862,716 + -1.6 862,716 + +0.6 862,716 + -0.4 862,716 + +0.6 862,716 + -0.0 862,716 + +1.2 862,717 - -0.5 862,717 - -0.3 862,717 - +0.9 862,717 - +0.1 862,717 - -0.1 862,717 - -1.4 862,717 - -0.0 862,717 - +1.3 862,717 - -0.0 862,717 - +0.9 862,717 - -0.6 862,717 - +0.1 862,717 - +0.2 862,717 - -0.3 862,717 - +0.3 862,717 - -1.6 862,717 - -0.7 862,717 - +1.6 862,732 - -0.9 862,786 + -0.1 862,787 - +0.3 862,828 + EX31_RS21095 0.12 +0.1 862,887 - EX31_RS21095 0.17 -0.7 862,887 - EX31_RS21095 0.17 +1.4 862,924 + EX31_RS21095 0.19 +0.7 862,924 + EX31_RS21095 0.19 +0.8 862,924 + EX31_RS21095 0.19 -0.5 862,924 + EX31_RS21095 0.19 +1.3 862,925 - EX31_RS21095 0.20 -0.0 862,947 + EX31_RS21095 0.21 -0.5 862,947 + EX31_RS21095 0.21 +0.4 862,947 + EX31_RS21095 0.21 -1.9 862,947 + EX31_RS21095 0.21 +0.4 862,948 - EX31_RS21095 0.21 -2.3 862,948 - EX31_RS21095 0.21 +0.6 863,031 - EX31_RS21095 0.28 +0.0 863,055 - EX31_RS21095 0.30 +0.1 863,055 - EX31_RS21095 0.30 -0.3 863,161 + EX31_RS21095 0.38 -0.4 863,161 + EX31_RS21095 0.38 -1.9
Or see this region's nucleotide sequence