Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18975 and EX31_RS18980 are separated by 108 nucleotides EX31_RS18980 and EX31_RS18985 overlap by 4 nucleotides EX31_RS18985 and EX31_RS18990 are separated by 228 nucleotides
EX31_RS18975: EX31_RS18975 - glutamine ABC transporter substrate-binding protein GlnH, at 383,235 to 383,978
_RS18975
EX31_RS18980: EX31_RS18980 - glutamine ABC transporter permease GlnP, at 384,087 to 384,746
_RS18980
EX31_RS18985: EX31_RS18985 - glutamine ABC transporter ATP-binding protein GlnQ, at 384,743 to 385,465
_RS18985
EX31_RS18990: EX31_RS18990 - PAS domain S-box protein, at 385,694 to 387,004
_RS18990
Position (kb) 384
385 Strain fitness (log2 ratio) -2
-1
0
1
2 at 383.102 kb on + strand at 383.154 kb on - strand at 383.191 kb on + strand at 383.195 kb on - strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.213 kb on + strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.214 kb on - strand at 383.217 kb on + strand at 383.217 kb on + strand at 383.218 kb on - strand at 383.248 kb on + strand at 383.270 kb on - strand at 383.306 kb on + strand at 383.321 kb on - strand, within EX31_RS18975 at 383.321 kb on - strand, within EX31_RS18975 at 383.324 kb on + strand, within EX31_RS18975 at 383.325 kb on - strand, within EX31_RS18975 at 383.341 kb on + strand, within EX31_RS18975 at 383.366 kb on + strand, within EX31_RS18975 at 383.366 kb on + strand, within EX31_RS18975 at 383.367 kb on - strand, within EX31_RS18975 at 383.370 kb on + strand, within EX31_RS18975 at 383.370 kb on + strand, within EX31_RS18975 at 383.370 kb on + strand, within EX31_RS18975 at 383.370 kb on + strand, within EX31_RS18975 at 383.370 kb on + strand, within EX31_RS18975 at 383.371 kb on - strand, within EX31_RS18975 at 383.371 kb on - strand, within EX31_RS18975 at 383.371 kb on - strand, within EX31_RS18975 at 383.371 kb on - strand, within EX31_RS18975 at 383.427 kb on + strand, within EX31_RS18975 at 383.427 kb on + strand, within EX31_RS18975 at 383.427 kb on + strand, within EX31_RS18975 at 383.428 kb on - strand, within EX31_RS18975 at 383.556 kb on + strand, within EX31_RS18975 at 383.556 kb on + strand, within EX31_RS18975 at 383.582 kb on + strand, within EX31_RS18975 at 383.582 kb on + strand, within EX31_RS18975 at 383.582 kb on + strand, within EX31_RS18975 at 383.628 kb on - strand, within EX31_RS18975 at 383.667 kb on + strand, within EX31_RS18975 at 383.668 kb on - strand, within EX31_RS18975 at 383.668 kb on - strand, within EX31_RS18975 at 383.668 kb on - strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.727 kb on + strand, within EX31_RS18975 at 383.728 kb on - strand, within EX31_RS18975 at 383.728 kb on - strand, within EX31_RS18975 at 383.728 kb on - strand, within EX31_RS18975 at 383.728 kb on - strand, within EX31_RS18975 at 383.787 kb on + strand, within EX31_RS18975 at 383.787 kb on + strand, within EX31_RS18975 at 383.787 kb on + strand, within EX31_RS18975 at 383.788 kb on - strand, within EX31_RS18975 at 383.788 kb on - strand, within EX31_RS18975 at 383.788 kb on - strand, within EX31_RS18975 at 383.788 kb on - strand, within EX31_RS18975 at 383.853 kb on + strand, within EX31_RS18975 at 383.854 kb on - strand, within EX31_RS18975 at 383.930 kb on + strand at 383.930 kb on + strand at 383.930 kb on + strand at 383.930 kb on + strand at 383.930 kb on + strand at 383.930 kb on + strand at 383.930 kb on + strand at 383.930 kb on + strand at 383.931 kb on - strand at 383.931 kb on - strand at 383.931 kb on - strand at 383.934 kb on + strand at 383.934 kb on + strand at 383.934 kb on + strand at 383.934 kb on + strand at 383.934 kb on + strand at 383.935 kb on - strand at 383.935 kb on - strand at 383.935 kb on - strand at 383.935 kb on - strand at 383.935 kb on - strand at 383.935 kb on - strand at 383.972 kb on + strand at 383.972 kb on + strand at 383.973 kb on - strand at 383.973 kb on - strand at 383.976 kb on + strand at 383.976 kb on + strand at 383.976 kb on + strand at 383.976 kb on + strand at 383.976 kb on + strand at 383.976 kb on + strand at 383.976 kb on + strand at 383.977 kb on - strand at 383.977 kb on - strand at 383.991 kb on + strand at 383.991 kb on + strand at 383.992 kb on - strand at 384.071 kb on + strand at 384.072 kb on - strand at 384.082 kb on - strand at 384.106 kb on + strand at 384.106 kb on + strand at 384.106 kb on + strand at 384.106 kb on + strand at 384.191 kb on + strand, within EX31_RS18980 at 384.191 kb on + strand, within EX31_RS18980 at 384.293 kb on - strand, within EX31_RS18980 at 384.299 kb on - strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.396 kb on + strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.397 kb on - strand, within EX31_RS18980 at 384.398 kb on + strand, within EX31_RS18980 at 384.398 kb on + strand, within EX31_RS18980 at 384.398 kb on + strand, within EX31_RS18980 at 384.399 kb on - strand, within EX31_RS18980 at 384.399 kb on - strand, within EX31_RS18980 at 384.399 kb on - strand, within EX31_RS18980 at 384.410 kb on + strand, within EX31_RS18980 at 384.495 kb on + strand, within EX31_RS18980 at 384.495 kb on + strand, within EX31_RS18980 at 384.495 kb on + strand, within EX31_RS18980 at 384.496 kb on - strand, within EX31_RS18980 at 384.560 kb on + strand, within EX31_RS18980 at 384.570 kb on - strand, within EX31_RS18980 at 384.674 kb on + strand, within EX31_RS18980 at 384.674 kb on + strand, within EX31_RS18980 at 384.674 kb on + strand, within EX31_RS18980 at 384.674 kb on + strand, within EX31_RS18980 at 384.674 kb on + strand, within EX31_RS18980 at 384.675 kb on - strand, within EX31_RS18980 at 384.678 kb on + strand, within EX31_RS18980 at 384.678 kb on + strand, within EX31_RS18980 at 384.678 kb on + strand, within EX31_RS18980 at 384.679 kb on - strand, within EX31_RS18980 at 384.679 kb on - strand, within EX31_RS18980 at 384.679 kb on - strand, within EX31_RS18980 at 384.679 kb on - strand, within EX31_RS18980 at 384.722 kb on - strand at 384.722 kb on - strand at 384.740 kb on + strand at 385.001 kb on + strand, within EX31_RS18985 at 385.001 kb on + strand, within EX31_RS18985 at 385.001 kb on + strand, within EX31_RS18985 at 385.381 kb on + strand, within EX31_RS18985 at 385.381 kb on + strand, within EX31_RS18985 at 385.494 kb on + strand at 385.495 kb on - strand at 385.495 kb on - strand at 385.643 kb on - strand at 385.695 kb on + strand at 385.695 kb on + strand at 385.695 kb on + strand at 385.695 kb on + strand at 385.696 kb on - strand at 385.696 kb on - strand at 385.696 kb on - strand at 385.696 kb on - strand at 385.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 383,102 + +0.1 383,154 - +0.2 383,191 + +0.4 383,195 - -0.8 383,213 + -1.4 383,213 + -1.5 383,213 + -0.0 383,213 + -0.2 383,213 + +0.2 383,213 + +0.0 383,213 + +0.8 383,213 + -1.1 383,213 + +0.4 383,214 - -0.3 383,214 - +1.0 383,214 - -0.2 383,214 - -1.2 383,214 - -0.1 383,214 - -0.2 383,214 - -0.1 383,214 - +0.3 383,214 - -1.1 383,214 - +0.2 383,214 - +0.0 383,214 - +0.8 383,214 - -0.3 383,214 - -0.3 383,214 - -0.6 383,214 - +0.2 383,217 + -0.2 383,217 + -0.3 383,218 - -0.1 383,248 + -0.0 383,270 - +2.1 383,306 + +0.2 383,321 - EX31_RS18975 0.12 +1.1 383,321 - EX31_RS18975 0.12 +0.6 383,324 + EX31_RS18975 0.12 -0.4 383,325 - EX31_RS18975 0.12 +0.2 383,341 + EX31_RS18975 0.14 -0.2 383,366 + EX31_RS18975 0.18 -0.1 383,366 + EX31_RS18975 0.18 +0.3 383,367 - EX31_RS18975 0.18 +0.7 383,370 + EX31_RS18975 0.18 -0.3 383,370 + EX31_RS18975 0.18 +1.1 383,370 + EX31_RS18975 0.18 -0.3 383,370 + EX31_RS18975 0.18 +0.4 383,370 + EX31_RS18975 0.18 +0.4 383,371 - EX31_RS18975 0.18 -0.7 383,371 - EX31_RS18975 0.18 -0.7 383,371 - EX31_RS18975 0.18 +0.5 383,371 - EX31_RS18975 0.18 +1.2 383,427 + EX31_RS18975 0.26 +0.0 383,427 + EX31_RS18975 0.26 -0.6 383,427 + EX31_RS18975 0.26 +1.0 383,428 - EX31_RS18975 0.26 +0.9 383,556 + EX31_RS18975 0.43 -0.4 383,556 + EX31_RS18975 0.43 +0.2 383,582 + EX31_RS18975 0.47 +0.6 383,582 + EX31_RS18975 0.47 +0.7 383,582 + EX31_RS18975 0.47 -0.4 383,628 - EX31_RS18975 0.53 +1.0 383,667 + EX31_RS18975 0.58 +0.7 383,668 - EX31_RS18975 0.58 +2.1 383,668 - EX31_RS18975 0.58 +0.2 383,668 - EX31_RS18975 0.58 +1.6 383,727 + EX31_RS18975 0.66 +0.4 383,727 + EX31_RS18975 0.66 -0.3 383,727 + EX31_RS18975 0.66 -0.3 383,727 + EX31_RS18975 0.66 -0.0 383,727 + EX31_RS18975 0.66 +0.4 383,727 + EX31_RS18975 0.66 -0.9 383,727 + EX31_RS18975 0.66 -0.3 383,728 - EX31_RS18975 0.66 +1.0 383,728 - EX31_RS18975 0.66 +0.2 383,728 - EX31_RS18975 0.66 +0.5 383,728 - EX31_RS18975 0.66 -0.0 383,787 + EX31_RS18975 0.74 +0.1 383,787 + EX31_RS18975 0.74 -0.7 383,787 + EX31_RS18975 0.74 +0.5 383,788 - EX31_RS18975 0.74 +0.1 383,788 - EX31_RS18975 0.74 -1.0 383,788 - EX31_RS18975 0.74 -0.2 383,788 - EX31_RS18975 0.74 +0.4 383,853 + EX31_RS18975 0.83 +0.1 383,854 - EX31_RS18975 0.83 +0.2 383,930 + +0.5 383,930 + -0.1 383,930 + +0.0 383,930 + +0.2 383,930 + +1.1 383,930 + -0.7 383,930 + -0.7 383,930 + -0.1 383,931 - +0.4 383,931 - +0.3 383,931 - +1.3 383,934 + +0.1 383,934 + -1.6 383,934 + +0.7 383,934 + -1.0 383,934 + +0.1 383,935 - -0.9 383,935 - +0.3 383,935 - +1.0 383,935 - +0.6 383,935 - +0.1 383,935 - -0.6 383,972 + -0.6 383,972 + +0.1 383,973 - +0.1 383,973 - -0.5 383,976 + -0.3 383,976 + -0.3 383,976 + +0.5 383,976 + +1.3 383,976 + -0.2 383,976 + +0.1 383,976 + -0.7 383,977 - -1.0 383,977 - -0.5 383,991 + -1.5 383,991 + +0.2 383,992 - -1.0 384,071 + +1.3 384,072 - +0.1 384,082 - -0.7 384,106 + +0.1 384,106 + +1.5 384,106 + -0.1 384,106 + +1.0 384,191 + EX31_RS18980 0.16 +1.0 384,191 + EX31_RS18980 0.16 +0.9 384,293 - EX31_RS18980 0.31 -0.6 384,299 - EX31_RS18980 0.32 -1.0 384,396 + EX31_RS18980 0.47 -0.2 384,396 + EX31_RS18980 0.47 +1.4 384,396 + EX31_RS18980 0.47 +0.0 384,396 + EX31_RS18980 0.47 -0.3 384,396 + EX31_RS18980 0.47 +0.5 384,396 + EX31_RS18980 0.47 -0.7 384,396 + EX31_RS18980 0.47 -0.3 384,396 + EX31_RS18980 0.47 -0.4 384,396 + EX31_RS18980 0.47 -0.2 384,396 + EX31_RS18980 0.47 -2.0 384,396 + EX31_RS18980 0.47 -1.8 384,396 + EX31_RS18980 0.47 -2.0 384,396 + EX31_RS18980 0.47 +0.4 384,396 + EX31_RS18980 0.47 -0.6 384,396 + EX31_RS18980 0.47 -0.4 384,397 - EX31_RS18980 0.47 +0.7 384,397 - EX31_RS18980 0.47 -2.4 384,397 - EX31_RS18980 0.47 +0.6 384,397 - EX31_RS18980 0.47 -1.7 384,397 - EX31_RS18980 0.47 -0.4 384,397 - EX31_RS18980 0.47 +0.3 384,397 - EX31_RS18980 0.47 +0.4 384,397 - EX31_RS18980 0.47 -0.5 384,397 - EX31_RS18980 0.47 -0.6 384,397 - EX31_RS18980 0.47 +0.4 384,398 + EX31_RS18980 0.47 +0.8 384,398 + EX31_RS18980 0.47 +0.9 384,398 + EX31_RS18980 0.47 +1.3 384,399 - EX31_RS18980 0.47 +1.4 384,399 - EX31_RS18980 0.47 +0.1 384,399 - EX31_RS18980 0.47 -1.1 384,410 + EX31_RS18980 0.49 -0.7 384,495 + EX31_RS18980 0.62 +1.1 384,495 + EX31_RS18980 0.62 +0.5 384,495 + EX31_RS18980 0.62 +1.0 384,496 - EX31_RS18980 0.62 -0.3 384,560 + EX31_RS18980 0.72 -0.2 384,570 - EX31_RS18980 0.73 +0.1 384,674 + EX31_RS18980 0.89 -0.5 384,674 + EX31_RS18980 0.89 -0.0 384,674 + EX31_RS18980 0.89 -0.3 384,674 + EX31_RS18980 0.89 -0.2 384,674 + EX31_RS18980 0.89 -0.5 384,675 - EX31_RS18980 0.89 -0.1 384,678 + EX31_RS18980 0.90 -0.7 384,678 + EX31_RS18980 0.90 +0.2 384,678 + EX31_RS18980 0.90 -0.1 384,679 - EX31_RS18980 0.90 +0.7 384,679 - EX31_RS18980 0.90 -0.7 384,679 - EX31_RS18980 0.90 -0.5 384,679 - EX31_RS18980 0.90 -0.8 384,722 - -1.0 384,722 - +0.5 384,740 + -1.3 385,001 + EX31_RS18985 0.36 +1.0 385,001 + EX31_RS18985 0.36 +0.2 385,001 + EX31_RS18985 0.36 -1.5 385,381 + EX31_RS18985 0.88 -0.6 385,381 + EX31_RS18985 0.88 -0.9 385,494 + -0.7 385,495 - -0.2 385,495 - +0.3 385,643 - +0.6 385,695 + +0.1 385,695 + +1.4 385,695 + -2.1 385,695 + -0.1 385,696 - +0.1 385,696 - -0.2 385,696 - +0.6 385,696 - -1.1 385,696 - -0.1
Or see this region's nucleotide sequence