Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18795 and EX31_RS18800 are separated by 3 nucleotides EX31_RS18800 and EX31_RS18805 are separated by 101 nucleotides EX31_RS18805 and EX31_RS18810 are separated by 224 nucleotides EX31_RS18810 and EX31_RS18815 are separated by 90 nucleotides
EX31_RS18795: EX31_RS18795 - DMT family transporter, at 343,792 to 344,244
_RS18795
EX31_RS18800: EX31_RS18800 - DMT family transporter, at 344,248 to 344,682
_RS18800
EX31_RS18805: EX31_RS18805 - LysR family transcriptional regulator, at 344,784 to 345,713
_RS18805
EX31_RS18810: EX31_RS18810 - GNAT family N-acetyltransferase, at 345,938 to 346,405
_RS18810
EX31_RS18815: EX31_RS18815 - choline dehydrogenase, at 346,496 to 348,181
_RS18815
Position (kb) 344
345
346 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 343.839 kb on + strand, within EX31_RS18795 at 343.839 kb on + strand, within EX31_RS18795 at 343.840 kb on - strand, within EX31_RS18795 at 344.006 kb on + strand, within EX31_RS18795 at 344.006 kb on + strand, within EX31_RS18795 at 344.006 kb on + strand, within EX31_RS18795 at 344.006 kb on + strand, within EX31_RS18795 at 344.006 kb on + strand, within EX31_RS18795 at 344.007 kb on - strand, within EX31_RS18795 at 344.007 kb on - strand, within EX31_RS18795 at 344.007 kb on - strand, within EX31_RS18795 at 344.020 kb on + strand, within EX31_RS18795 at 344.020 kb on + strand, within EX31_RS18795 at 344.104 kb on - strand, within EX31_RS18795 at 344.165 kb on - strand, within EX31_RS18795 at 344.191 kb on - strand, within EX31_RS18795 at 344.221 kb on + strand at 344.222 kb on - strand at 344.222 kb on - strand at 344.224 kb on + strand at 344.224 kb on + strand at 344.225 kb on - strand at 344.225 kb on - strand at 344.225 kb on - strand at 344.346 kb on - strand, within EX31_RS18800 at 344.447 kb on + strand, within EX31_RS18800 at 344.447 kb on + strand, within EX31_RS18800 at 344.447 kb on + strand, within EX31_RS18800 at 344.447 kb on + strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.448 kb on - strand, within EX31_RS18800 at 344.568 kb on + strand, within EX31_RS18800 at 344.568 kb on + strand, within EX31_RS18800 at 344.569 kb on - strand, within EX31_RS18800 at 344.718 kb on + strand at 344.718 kb on + strand at 344.720 kb on + strand at 344.720 kb on + strand at 344.720 kb on + strand at 344.720 kb on + strand at 344.721 kb on - strand at 344.721 kb on - strand at 344.762 kb on + strand at 344.763 kb on - strand at 344.775 kb on + strand at 344.795 kb on + strand at 344.807 kb on + strand at 344.808 kb on - strand at 344.808 kb on - strand at 344.819 kb on - strand at 344.826 kb on + strand at 344.826 kb on + strand at 344.826 kb on + strand at 344.826 kb on + strand at 344.826 kb on + strand at 344.827 kb on - strand at 344.929 kb on + strand, within EX31_RS18805 at 344.929 kb on + strand, within EX31_RS18805 at 344.929 kb on + strand, within EX31_RS18805 at 344.929 kb on + strand, within EX31_RS18805 at 344.929 kb on + strand, within EX31_RS18805 at 344.946 kb on + strand, within EX31_RS18805 at 345.143 kb on + strand, within EX31_RS18805 at 345.144 kb on - strand, within EX31_RS18805 at 345.192 kb on - strand, within EX31_RS18805 at 345.309 kb on + strand, within EX31_RS18805 at 345.309 kb on + strand, within EX31_RS18805 at 345.402 kb on + strand, within EX31_RS18805 at 345.451 kb on + strand, within EX31_RS18805 at 345.464 kb on + strand, within EX31_RS18805 at 345.465 kb on - strand, within EX31_RS18805 at 345.465 kb on - strand, within EX31_RS18805 at 345.465 kb on - strand, within EX31_RS18805 at 345.580 kb on - strand, within EX31_RS18805 at 345.586 kb on + strand, within EX31_RS18805 at 345.740 kb on + strand at 345.741 kb on - strand at 345.741 kb on - strand at 345.939 kb on + strand at 345.940 kb on - strand at 345.940 kb on - strand at 345.956 kb on + strand at 346.029 kb on + strand, within EX31_RS18810 at 346.207 kb on - strand, within EX31_RS18810 at 346.240 kb on + strand, within EX31_RS18810 at 346.284 kb on + strand, within EX31_RS18810 at 346.310 kb on + strand, within EX31_RS18810 at 346.310 kb on + strand, within EX31_RS18810 at 346.311 kb on - strand, within EX31_RS18810 at 346.311 kb on - strand, within EX31_RS18810 at 346.338 kb on + strand, within EX31_RS18810 at 346.338 kb on + strand, within EX31_RS18810 at 346.339 kb on - strand, within EX31_RS18810 at 346.371 kb on + strand at 346.372 kb on - strand at 346.372 kb on - strand at 346.372 kb on - strand at 346.450 kb on - strand at 346.450 kb on - strand at 346.462 kb on - strand at 346.494 kb on + strand at 346.548 kb on + strand at 346.549 kb on - strand at 346.549 kb on - strand at 346.549 kb on - strand at 346.552 kb on - strand at 346.552 kb on - strand at 346.595 kb on - strand at 346.619 kb on - strand at 346.621 kb on + strand at 346.621 kb on + strand at 346.622 kb on - strand at 346.622 kb on - strand at 346.622 kb on - strand at 346.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 343,839 + EX31_RS18795 0.10 -0.1 343,839 + EX31_RS18795 0.10 -1.9 343,840 - EX31_RS18795 0.11 -0.2 344,006 + EX31_RS18795 0.47 +1.9 344,006 + EX31_RS18795 0.47 -0.3 344,006 + EX31_RS18795 0.47 +1.8 344,006 + EX31_RS18795 0.47 -0.3 344,006 + EX31_RS18795 0.47 -0.5 344,007 - EX31_RS18795 0.47 -0.1 344,007 - EX31_RS18795 0.47 -0.0 344,007 - EX31_RS18795 0.47 -1.4 344,020 + EX31_RS18795 0.50 +0.6 344,020 + EX31_RS18795 0.50 -1.4 344,104 - EX31_RS18795 0.69 +1.9 344,165 - EX31_RS18795 0.82 +1.3 344,191 - EX31_RS18795 0.88 -0.0 344,221 + +0.9 344,222 - +0.8 344,222 - +1.0 344,224 + -0.9 344,224 + +2.1 344,225 - +0.1 344,225 - +0.2 344,225 - -1.4 344,346 - EX31_RS18800 0.23 -0.2 344,447 + EX31_RS18800 0.46 +0.3 344,447 + EX31_RS18800 0.46 -0.1 344,447 + EX31_RS18800 0.46 -0.9 344,447 + EX31_RS18800 0.46 +0.2 344,448 - EX31_RS18800 0.46 +0.8 344,448 - EX31_RS18800 0.46 -0.2 344,448 - EX31_RS18800 0.46 +0.1 344,448 - EX31_RS18800 0.46 -0.4 344,448 - EX31_RS18800 0.46 +0.2 344,448 - EX31_RS18800 0.46 +0.5 344,448 - EX31_RS18800 0.46 -0.4 344,568 + EX31_RS18800 0.74 +0.1 344,568 + EX31_RS18800 0.74 +1.7 344,569 - EX31_RS18800 0.74 +0.4 344,718 + -0.3 344,718 + +1.5 344,720 + -0.1 344,720 + -1.2 344,720 + +0.6 344,720 + +1.0 344,721 - -0.1 344,721 - +0.9 344,762 + +1.6 344,763 - -0.5 344,775 + +0.1 344,795 + -0.3 344,807 + +0.1 344,808 - -0.9 344,808 - -1.7 344,819 - -0.7 344,826 + -1.1 344,826 + -0.5 344,826 + +0.6 344,826 + -1.0 344,826 + +1.1 344,827 - -1.1 344,929 + EX31_RS18805 0.16 +0.7 344,929 + EX31_RS18805 0.16 -1.1 344,929 + EX31_RS18805 0.16 +0.6 344,929 + EX31_RS18805 0.16 +0.8 344,929 + EX31_RS18805 0.16 +0.5 344,946 + EX31_RS18805 0.17 +1.4 345,143 + EX31_RS18805 0.39 +2.1 345,144 - EX31_RS18805 0.39 +0.1 345,192 - EX31_RS18805 0.44 +1.1 345,309 + EX31_RS18805 0.56 -0.3 345,309 + EX31_RS18805 0.56 -0.9 345,402 + EX31_RS18805 0.66 -0.2 345,451 + EX31_RS18805 0.72 -0.6 345,464 + EX31_RS18805 0.73 -0.5 345,465 - EX31_RS18805 0.73 +0.3 345,465 - EX31_RS18805 0.73 -0.7 345,465 - EX31_RS18805 0.73 -0.0 345,580 - EX31_RS18805 0.86 +0.7 345,586 + EX31_RS18805 0.86 +0.8 345,740 + -0.3 345,741 - +0.5 345,741 - -0.1 345,939 + +0.1 345,940 - +0.3 345,940 - -0.5 345,956 + -1.2 346,029 + EX31_RS18810 0.19 +0.1 346,207 - EX31_RS18810 0.57 -0.9 346,240 + EX31_RS18810 0.65 -1.7 346,284 + EX31_RS18810 0.74 +0.6 346,310 + EX31_RS18810 0.79 -0.6 346,310 + EX31_RS18810 0.79 -1.1 346,311 - EX31_RS18810 0.80 +0.1 346,311 - EX31_RS18810 0.80 +0.2 346,338 + EX31_RS18810 0.85 -0.2 346,338 + EX31_RS18810 0.85 -0.3 346,339 - EX31_RS18810 0.86 +0.2 346,371 + -0.3 346,372 - -0.3 346,372 - +0.1 346,372 - +0.9 346,450 - -0.8 346,450 - -0.7 346,462 - -0.6 346,494 + +0.5 346,548 + +0.7 346,549 - -0.0 346,549 - +0.2 346,549 - +0.1 346,552 - +0.9 346,552 - -0.9 346,595 - -3.2 346,619 - -0.2 346,621 + +0.2 346,621 + +0.5 346,622 - +0.6 346,622 - -1.4 346,622 - +0.4 346,622 - -1.3
Or see this region's nucleotide sequence