Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17360 and EX31_RS17365 are separated by 60 nucleotides EX31_RS17365 and EX31_RS17370 are separated by 68 nucleotides EX31_RS17370 and EX31_RS17375 are separated by 125 nucleotides
EX31_RS17360: EX31_RS17360 - molecular chaperone, at 21,376 to 21,960
_RS17360
EX31_RS17365: EX31_RS17365 - phosphatase, at 22,021 to 22,761
_RS17365
EX31_RS17370: EX31_RS17370 - glyoxylate/hydroxypyruvate reductase GhrA, at 22,830 to 23,771
_RS17370
EX31_RS17375: EX31_RS17375 - MFS transporter, at 23,897 to 25,378
_RS17375
Position (kb) 22
23
24 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 21.915 kb on - strand at 21.960 kb on + strand at 21.961 kb on - strand at 21.961 kb on - strand at 21.966 kb on + strand at 21.980 kb on - strand at 21.992 kb on + strand at 22.034 kb on - strand at 22.105 kb on - strand, within EX31_RS17365 at 22.137 kb on + strand, within EX31_RS17365 at 22.138 kb on - strand, within EX31_RS17365 at 22.138 kb on - strand, within EX31_RS17365 at 22.138 kb on - strand, within EX31_RS17365 at 22.138 kb on - strand, within EX31_RS17365 at 22.219 kb on + strand, within EX31_RS17365 at 22.220 kb on - strand, within EX31_RS17365 at 22.284 kb on + strand, within EX31_RS17365 at 22.285 kb on - strand, within EX31_RS17365 at 22.285 kb on - strand, within EX31_RS17365 at 22.285 kb on - strand, within EX31_RS17365 at 22.305 kb on + strand, within EX31_RS17365 at 22.306 kb on - strand, within EX31_RS17365 at 22.307 kb on + strand, within EX31_RS17365 at 22.307 kb on + strand, within EX31_RS17365 at 22.308 kb on - strand, within EX31_RS17365 at 22.308 kb on - strand, within EX31_RS17365 at 22.338 kb on + strand, within EX31_RS17365 at 22.339 kb on - strand, within EX31_RS17365 at 22.350 kb on - strand, within EX31_RS17365 at 22.374 kb on + strand, within EX31_RS17365 at 22.374 kb on + strand, within EX31_RS17365 at 22.375 kb on - strand, within EX31_RS17365 at 22.471 kb on - strand, within EX31_RS17365 at 22.533 kb on + strand, within EX31_RS17365 at 22.600 kb on - strand, within EX31_RS17365 at 22.600 kb on - strand, within EX31_RS17365 at 22.662 kb on + strand, within EX31_RS17365 at 22.662 kb on + strand, within EX31_RS17365 at 22.662 kb on + strand, within EX31_RS17365 at 22.663 kb on - strand, within EX31_RS17365 at 22.691 kb on + strand at 22.691 kb on + strand at 22.691 kb on + strand at 22.691 kb on + strand at 22.691 kb on + strand at 22.692 kb on - strand at 22.692 kb on - strand at 22.692 kb on - strand at 22.692 kb on - strand at 22.692 kb on - strand at 22.692 kb on - strand at 22.699 kb on + strand at 22.699 kb on + strand at 22.699 kb on + strand at 22.699 kb on + strand at 22.700 kb on - strand at 22.700 kb on - strand at 22.700 kb on - strand at 22.700 kb on - strand at 22.700 kb on - strand at 22.709 kb on + strand at 22.710 kb on - strand at 22.731 kb on + strand at 22.737 kb on + strand at 22.737 kb on + strand at 22.738 kb on - strand at 22.738 kb on - strand at 22.738 kb on - strand at 22.738 kb on - strand at 22.738 kb on - strand at 22.738 kb on - strand at 22.738 kb on - strand at 22.741 kb on + strand at 22.741 kb on + strand at 22.741 kb on + strand at 22.741 kb on + strand at 22.742 kb on - strand at 22.742 kb on - strand at 22.742 kb on - strand at 22.742 kb on - strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.763 kb on + strand at 22.764 kb on - strand at 22.764 kb on - strand at 22.764 kb on - strand at 22.764 kb on - strand at 22.764 kb on - strand at 22.764 kb on - strand at 22.797 kb on - strand at 22.797 kb on - strand at 22.809 kb on + strand at 22.809 kb on + strand at 22.809 kb on + strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.812 kb on - strand at 22.812 kb on - strand at 22.812 kb on - strand at 22.817 kb on + strand at 22.818 kb on - strand at 22.818 kb on - strand at 22.952 kb on + strand, within EX31_RS17370 at 23.138 kb on + strand, within EX31_RS17370 at 23.139 kb on - strand, within EX31_RS17370 at 23.139 kb on - strand, within EX31_RS17370 at 23.233 kb on + strand, within EX31_RS17370 at 23.233 kb on + strand, within EX31_RS17370 at 23.234 kb on - strand, within EX31_RS17370 at 23.245 kb on - strand, within EX31_RS17370 at 23.245 kb on - strand, within EX31_RS17370 at 23.245 kb on - strand, within EX31_RS17370 at 23.298 kb on - strand, within EX31_RS17370 at 23.349 kb on - strand, within EX31_RS17370 at 23.440 kb on + strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.446 kb on - strand, within EX31_RS17370 at 23.449 kb on - strand, within EX31_RS17370 at 23.464 kb on + strand, within EX31_RS17370 at 23.464 kb on + strand, within EX31_RS17370 at 23.465 kb on - strand, within EX31_RS17370 at 23.515 kb on - strand, within EX31_RS17370 at 23.651 kb on - strand, within EX31_RS17370 at 23.716 kb on - strand at 23.747 kb on + strand at 23.747 kb on + strand at 23.747 kb on + strand at 23.747 kb on + strand at 23.747 kb on + strand at 23.747 kb on + strand at 23.748 kb on - strand at 23.748 kb on - strand at 23.748 kb on - strand at 23.748 kb on - strand at 23.796 kb on - strand at 23.944 kb on + strand at 23.996 kb on - strand at 24.069 kb on + strand, within EX31_RS17375 at 24.210 kb on + strand, within EX31_RS17375 at 24.210 kb on + strand, within EX31_RS17375 at 24.210 kb on + strand, within EX31_RS17375 at 24.211 kb on - strand, within EX31_RS17375 at 24.230 kb on - strand, within EX31_RS17375 at 24.230 kb on - strand, within EX31_RS17375 at 24.230 kb on - strand, within EX31_RS17375 at 24.299 kb on - strand, within EX31_RS17375 at 24.332 kb on - strand, within EX31_RS17375 at 24.409 kb on + strand, within EX31_RS17375 at 24.410 kb on - strand, within EX31_RS17375 at 24.410 kb on - strand, within EX31_RS17375 at 24.410 kb on - strand, within EX31_RS17375 at 24.410 kb on - strand, within EX31_RS17375 at 24.410 kb on - strand, within EX31_RS17375 at 24.410 kb on - strand, within EX31_RS17375 at 24.413 kb on + strand, within EX31_RS17375 at 24.413 kb on + strand, within EX31_RS17375 at 24.413 kb on + strand, within EX31_RS17375 at 24.414 kb on - strand, within EX31_RS17375 at 24.414 kb on - strand, within EX31_RS17375 at 24.414 kb on - strand, within EX31_RS17375 at 24.414 kb on - strand, within EX31_RS17375 at 24.414 kb on - strand, within EX31_RS17375 at 24.414 kb on - strand, within EX31_RS17375 at 24.450 kb on + strand, within EX31_RS17375 at 24.450 kb on + strand, within EX31_RS17375 at 24.451 kb on - strand, within EX31_RS17375 at 24.451 kb on - strand, within EX31_RS17375 at 24.451 kb on - strand, within EX31_RS17375 at 24.451 kb on - strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.452 kb on + strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.453 kb on - strand, within EX31_RS17375 at 24.510 kb on + strand, within EX31_RS17375 at 24.510 kb on + strand, within EX31_RS17375 at 24.510 kb on + strand, within EX31_RS17375 at 24.511 kb on - strand, within EX31_RS17375 at 24.511 kb on - strand, within EX31_RS17375 at 24.553 kb on - strand, within EX31_RS17375 at 24.612 kb on - strand, within EX31_RS17375 at 24.649 kb on - strand, within EX31_RS17375 at 24.699 kb on - strand, within EX31_RS17375 at 24.738 kb on - strand, within EX31_RS17375
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 21,915 - +0.4 21,960 + -1.3 21,961 - -0.2 21,961 - +0.8 21,966 + +0.6 21,980 - -1.4 21,992 + +0.2 22,034 - +1.4 22,105 - EX31_RS17365 0.11 -0.0 22,137 + EX31_RS17365 0.16 +0.9 22,138 - EX31_RS17365 0.16 +0.1 22,138 - EX31_RS17365 0.16 -1.7 22,138 - EX31_RS17365 0.16 +0.0 22,138 - EX31_RS17365 0.16 +1.1 22,219 + EX31_RS17365 0.27 +0.5 22,220 - EX31_RS17365 0.27 -0.8 22,284 + EX31_RS17365 0.35 -0.6 22,285 - EX31_RS17365 0.36 +0.1 22,285 - EX31_RS17365 0.36 -1.0 22,285 - EX31_RS17365 0.36 -0.3 22,305 + EX31_RS17365 0.38 +1.0 22,306 - EX31_RS17365 0.38 +0.8 22,307 + EX31_RS17365 0.39 -0.3 22,307 + EX31_RS17365 0.39 -0.7 22,308 - EX31_RS17365 0.39 +0.4 22,308 - EX31_RS17365 0.39 -0.3 22,338 + EX31_RS17365 0.43 +1.6 22,339 - EX31_RS17365 0.43 -0.2 22,350 - EX31_RS17365 0.44 +0.4 22,374 + EX31_RS17365 0.48 +1.6 22,374 + EX31_RS17365 0.48 -0.2 22,375 - EX31_RS17365 0.48 -0.6 22,471 - EX31_RS17365 0.61 +0.6 22,533 + EX31_RS17365 0.69 -0.1 22,600 - EX31_RS17365 0.78 -1.4 22,600 - EX31_RS17365 0.78 +0.4 22,662 + EX31_RS17365 0.87 +1.0 22,662 + EX31_RS17365 0.87 -2.2 22,662 + EX31_RS17365 0.87 +0.0 22,663 - EX31_RS17365 0.87 -0.3 22,691 + +0.3 22,691 + +1.2 22,691 + +0.4 22,691 + +0.3 22,691 + -0.3 22,692 - -0.1 22,692 - +0.4 22,692 - +0.9 22,692 - +1.3 22,692 - +0.6 22,692 - +0.0 22,699 + +1.1 22,699 + +1.4 22,699 + -1.9 22,699 + -0.4 22,700 - -1.0 22,700 - -0.3 22,700 - -0.4 22,700 - +0.8 22,700 - +0.2 22,709 + -0.1 22,710 - -0.6 22,731 + +0.0 22,737 + +1.1 22,737 + +0.2 22,738 - +1.7 22,738 - +0.8 22,738 - +1.5 22,738 - +0.0 22,738 - -0.0 22,738 - +1.5 22,738 - -0.2 22,741 + +1.0 22,741 + -0.5 22,741 + +2.0 22,741 + +0.6 22,742 - +0.2 22,742 - +3.0 22,742 - -0.6 22,742 - +0.5 22,763 + +1.4 22,763 + +0.9 22,763 + -0.2 22,763 + +0.1 22,763 + +0.3 22,763 + +0.6 22,763 + +0.0 22,763 + +1.0 22,764 - -1.3 22,764 - +2.0 22,764 - -0.6 22,764 - -0.3 22,764 - +0.6 22,764 - +1.3 22,797 - +1.1 22,797 - -1.3 22,809 + +0.9 22,809 + -0.3 22,809 + -0.3 22,810 - +0.1 22,810 - +0.7 22,810 - -0.7 22,810 - -0.4 22,810 - -1.3 22,810 - +0.2 22,810 - -1.6 22,812 - +0.2 22,812 - +0.0 22,812 - -1.1 22,817 + +1.0 22,818 - +0.2 22,818 - -0.6 22,952 + EX31_RS17370 0.13 -0.3 23,138 + EX31_RS17370 0.33 +0.0 23,139 - EX31_RS17370 0.33 +0.0 23,139 - EX31_RS17370 0.33 -0.7 23,233 + EX31_RS17370 0.43 -0.5 23,233 + EX31_RS17370 0.43 -0.3 23,234 - EX31_RS17370 0.43 -0.6 23,245 - EX31_RS17370 0.44 +1.4 23,245 - EX31_RS17370 0.44 +0.0 23,245 - EX31_RS17370 0.44 +0.4 23,298 - EX31_RS17370 0.50 -0.1 23,349 - EX31_RS17370 0.55 -0.6 23,440 + EX31_RS17370 0.65 -0.0 23,446 - EX31_RS17370 0.65 -1.4 23,446 - EX31_RS17370 0.65 -1.8 23,446 - EX31_RS17370 0.65 -0.3 23,446 - EX31_RS17370 0.65 -0.4 23,446 - EX31_RS17370 0.65 +0.6 23,446 - EX31_RS17370 0.65 -1.4 23,446 - EX31_RS17370 0.65 +0.8 23,449 - EX31_RS17370 0.66 +1.2 23,464 + EX31_RS17370 0.67 +0.7 23,464 + EX31_RS17370 0.67 +0.7 23,465 - EX31_RS17370 0.67 +0.6 23,515 - EX31_RS17370 0.73 +0.2 23,651 - EX31_RS17370 0.87 -0.7 23,716 - -0.7 23,747 + -0.2 23,747 + +1.0 23,747 + -1.4 23,747 + +1.6 23,747 + +0.4 23,747 + +1.6 23,748 - +1.0 23,748 - +1.0 23,748 - +0.4 23,748 - -2.4 23,796 - +1.4 23,944 + +2.6 23,996 - -0.1 24,069 + EX31_RS17375 0.12 +1.0 24,210 + EX31_RS17375 0.21 -0.5 24,210 + EX31_RS17375 0.21 -0.1 24,210 + EX31_RS17375 0.21 +0.0 24,211 - EX31_RS17375 0.21 -1.8 24,230 - EX31_RS17375 0.22 +0.4 24,230 - EX31_RS17375 0.22 +0.9 24,230 - EX31_RS17375 0.22 -0.4 24,299 - EX31_RS17375 0.27 -0.1 24,332 - EX31_RS17375 0.29 -0.1 24,409 + EX31_RS17375 0.35 +1.4 24,410 - EX31_RS17375 0.35 -0.3 24,410 - EX31_RS17375 0.35 -0.6 24,410 - EX31_RS17375 0.35 +0.2 24,410 - EX31_RS17375 0.35 -1.1 24,410 - EX31_RS17375 0.35 -0.7 24,410 - EX31_RS17375 0.35 +1.3 24,413 + EX31_RS17375 0.35 -1.4 24,413 + EX31_RS17375 0.35 -0.3 24,413 + EX31_RS17375 0.35 -1.0 24,414 - EX31_RS17375 0.35 +0.2 24,414 - EX31_RS17375 0.35 -0.3 24,414 - EX31_RS17375 0.35 -0.3 24,414 - EX31_RS17375 0.35 +1.3 24,414 - EX31_RS17375 0.35 +0.0 24,414 - EX31_RS17375 0.35 +0.3 24,450 + EX31_RS17375 0.37 +0.0 24,450 + EX31_RS17375 0.37 +4.0 24,451 - EX31_RS17375 0.37 -0.2 24,451 - EX31_RS17375 0.37 +0.7 24,451 - EX31_RS17375 0.37 -2.2 24,451 - EX31_RS17375 0.37 -0.2 24,452 + EX31_RS17375 0.37 -0.4 24,452 + EX31_RS17375 0.37 +1.4 24,452 + EX31_RS17375 0.37 +0.3 24,452 + EX31_RS17375 0.37 +0.0 24,452 + EX31_RS17375 0.37 -1.7 24,452 + EX31_RS17375 0.37 +0.8 24,452 + EX31_RS17375 0.37 +1.5 24,452 + EX31_RS17375 0.37 +1.0 24,452 + EX31_RS17375 0.37 +0.2 24,452 + EX31_RS17375 0.37 +0.7 24,452 + EX31_RS17375 0.37 +0.4 24,452 + EX31_RS17375 0.37 -0.7 24,453 - EX31_RS17375 0.38 -0.8 24,453 - EX31_RS17375 0.38 +0.2 24,453 - EX31_RS17375 0.38 -1.0 24,453 - EX31_RS17375 0.38 +0.8 24,453 - EX31_RS17375 0.38 -1.3 24,453 - EX31_RS17375 0.38 -0.2 24,453 - EX31_RS17375 0.38 -0.7 24,453 - EX31_RS17375 0.38 -0.1 24,453 - EX31_RS17375 0.38 -0.5 24,453 - EX31_RS17375 0.38 +1.0 24,453 - EX31_RS17375 0.38 -0.3 24,453 - EX31_RS17375 0.38 +0.2 24,453 - EX31_RS17375 0.38 -1.1 24,453 - EX31_RS17375 0.38 +1.0 24,453 - EX31_RS17375 0.38 +1.0 24,453 - EX31_RS17375 0.38 +1.0 24,453 - EX31_RS17375 0.38 +0.4 24,453 - EX31_RS17375 0.38 +0.8 24,453 - EX31_RS17375 0.38 -0.5 24,453 - EX31_RS17375 0.38 -0.1 24,453 - EX31_RS17375 0.38 +1.6 24,453 - EX31_RS17375 0.38 -0.1 24,453 - EX31_RS17375 0.38 +0.0 24,453 - EX31_RS17375 0.38 +1.5 24,453 - EX31_RS17375 0.38 -0.3 24,453 - EX31_RS17375 0.38 -0.6 24,453 - EX31_RS17375 0.38 -0.8 24,510 + EX31_RS17375 0.41 -0.4 24,510 + EX31_RS17375 0.41 -2.1 24,510 + EX31_RS17375 0.41 -0.2 24,511 - EX31_RS17375 0.41 +0.3 24,511 - EX31_RS17375 0.41 +0.3 24,553 - EX31_RS17375 0.44 +0.8 24,612 - EX31_RS17375 0.48 -0.2 24,649 - EX31_RS17375 0.51 +0.0 24,699 - EX31_RS17375 0.54 -0.6 24,738 - EX31_RS17375 0.57 -2.0
Or see this region's nucleotide sequence