Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10250 and EX31_RS10255 are separated by 394 nucleotides EX31_RS10255 and EX31_RS10260 are separated by 70 nucleotides EX31_RS10260 and EX31_RS10265 overlap by 4 nucleotides
EX31_RS10250: EX31_RS10250 - MurR/RpiR family transcriptional regulator, at 263,402 to 264,274
_RS10250
EX31_RS10255: EX31_RS10255 - tRNA-Asn, at 264,669 to 264,744
_RS10255
EX31_RS10260: EX31_RS10260 - hypothetical protein, at 264,815 to 265,009
_RS10260
EX31_RS10265: EX31_RS10265 - alpha-glucosidase, at 265,006 to 266,664
_RS10265
Position (kb) 264
265
266 Strain fitness (log2 ratio) -2
-1
0
1
2 at 263.913 kb on + strand, within EX31_RS10250 at 264.328 kb on - strand at 264.328 kb on - strand at 264.359 kb on - strand at 264.359 kb on - strand at 264.453 kb on - strand at 264.505 kb on + strand at 264.550 kb on + strand at 264.745 kb on + strand at 264.745 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.748 kb on + strand at 264.792 kb on + strand at 264.793 kb on - strand at 264.793 kb on - strand at 264.815 kb on - strand at 264.846 kb on + strand, within EX31_RS10260 at 264.931 kb on + strand, within EX31_RS10260 at 264.977 kb on + strand, within EX31_RS10260 at 264.977 kb on + strand, within EX31_RS10260 at 264.978 kb on - strand, within EX31_RS10260 at 265.008 kb on - strand at 265.017 kb on - strand at 265.067 kb on + strand at 265.067 kb on + strand at 265.067 kb on + strand at 265.068 kb on - strand at 265.068 kb on - strand at 265.072 kb on - strand at 265.079 kb on - strand at 265.177 kb on - strand, within EX31_RS10265 at 265.184 kb on + strand, within EX31_RS10265 at 265.190 kb on + strand, within EX31_RS10265 at 265.190 kb on + strand, within EX31_RS10265 at 265.191 kb on - strand, within EX31_RS10265 at 265.191 kb on - strand, within EX31_RS10265 at 265.191 kb on - strand, within EX31_RS10265 at 265.191 kb on - strand, within EX31_RS10265 at 265.223 kb on + strand, within EX31_RS10265 at 265.223 kb on + strand, within EX31_RS10265 at 265.224 kb on - strand, within EX31_RS10265 at 265.224 kb on - strand, within EX31_RS10265 at 265.299 kb on + strand, within EX31_RS10265 at 265.389 kb on + strand, within EX31_RS10265 at 265.572 kb on - strand, within EX31_RS10265 at 265.575 kb on + strand, within EX31_RS10265 at 265.575 kb on + strand, within EX31_RS10265 at 265.601 kb on + strand, within EX31_RS10265 at 265.602 kb on - strand, within EX31_RS10265 at 265.606 kb on - strand, within EX31_RS10265 at 265.694 kb on + strand, within EX31_RS10265 at 265.695 kb on - strand, within EX31_RS10265 at 265.695 kb on - strand, within EX31_RS10265 at 265.695 kb on - strand, within EX31_RS10265 at 265.747 kb on - strand, within EX31_RS10265 at 265.754 kb on - strand, within EX31_RS10265 at 265.814 kb on - strand, within EX31_RS10265 at 265.958 kb on + strand, within EX31_RS10265 at 265.958 kb on + strand, within EX31_RS10265 at 265.976 kb on + strand, within EX31_RS10265 at 266.006 kb on + strand, within EX31_RS10265
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 263,913 + EX31_RS10250 0.59 -0.7 264,328 - -0.6 264,328 - -1.1 264,359 - +1.9 264,359 - -0.5 264,453 - -0.3 264,505 + -1.7 264,550 + +1.7 264,745 + +0.7 264,745 + -0.4 264,748 + -0.5 264,748 + +1.9 264,748 + +0.7 264,748 + -0.1 264,748 + -0.9 264,748 + +0.5 264,748 + +0.1 264,748 + +0.1 264,748 + -0.6 264,748 + -0.3 264,748 + +0.3 264,748 + -0.5 264,792 + +0.6 264,793 - +0.9 264,793 - -0.4 264,815 - +0.9 264,846 + EX31_RS10260 0.16 -2.4 264,931 + EX31_RS10260 0.59 +0.5 264,977 + EX31_RS10260 0.83 -1.7 264,977 + EX31_RS10260 0.83 +1.3 264,978 - EX31_RS10260 0.84 -0.8 265,008 - -0.4 265,017 - -0.7 265,067 + +1.9 265,067 + +1.5 265,067 + +0.7 265,068 - +0.3 265,068 - +0.7 265,072 - +1.4 265,079 - -0.5 265,177 - EX31_RS10265 0.10 -1.1 265,184 + EX31_RS10265 0.11 +0.2 265,190 + EX31_RS10265 0.11 +0.1 265,190 + EX31_RS10265 0.11 +0.2 265,191 - EX31_RS10265 0.11 +1.3 265,191 - EX31_RS10265 0.11 +0.1 265,191 - EX31_RS10265 0.11 +1.7 265,191 - EX31_RS10265 0.11 +0.7 265,223 + EX31_RS10265 0.13 -1.1 265,223 + EX31_RS10265 0.13 +1.5 265,224 - EX31_RS10265 0.13 -0.4 265,224 - EX31_RS10265 0.13 +0.3 265,299 + EX31_RS10265 0.18 +0.6 265,389 + EX31_RS10265 0.23 -0.7 265,572 - EX31_RS10265 0.34 -0.5 265,575 + EX31_RS10265 0.34 -0.4 265,575 + EX31_RS10265 0.34 +1.7 265,601 + EX31_RS10265 0.36 +1.2 265,602 - EX31_RS10265 0.36 -0.1 265,606 - EX31_RS10265 0.36 +1.3 265,694 + EX31_RS10265 0.41 +0.6 265,695 - EX31_RS10265 0.42 -1.0 265,695 - EX31_RS10265 0.42 +0.9 265,695 - EX31_RS10265 0.42 +0.1 265,747 - EX31_RS10265 0.45 -0.1 265,754 - EX31_RS10265 0.45 -0.6 265,814 - EX31_RS10265 0.49 -0.4 265,958 + EX31_RS10265 0.57 +0.4 265,958 + EX31_RS10265 0.57 -0.9 265,976 + EX31_RS10265 0.58 +1.9 266,006 + EX31_RS10265 0.60 -0.5
Or see this region's nucleotide sequence