Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09875 and EX31_RS09880 are separated by 290 nucleotides EX31_RS09880 and EX31_RS09885 are separated by 73 nucleotides EX31_RS09885 and EX31_RS09890 are separated by 191 nucleotides
EX31_RS09875: EX31_RS09875 - phosphatidylglycerophosphatase B, at 181,117 to 181,872
_RS09875
EX31_RS09880: EX31_RS09880 - GTP cyclohydrolase II, at 182,163 to 182,753
_RS09880
EX31_RS09885: EX31_RS09885 - aconitate hydratase AcnA, at 182,827 to 185,499
_RS09885
EX31_RS09890: EX31_RS09890 - HTH-type transcriptional regulator CysB, at 185,691 to 186,665
_RS09890
Position (kb) 182
183
184
185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 181.872 kb on + strand at 181.873 kb on - strand at 181.873 kb on - strand at 181.873 kb on - strand at 181.873 kb on - strand at 181.894 kb on + strand at 181.911 kb on + strand at 181.911 kb on + strand at 181.912 kb on - strand at 181.912 kb on - strand at 181.930 kb on + strand at 181.966 kb on + strand at 181.968 kb on + strand at 181.975 kb on + strand at 181.975 kb on + strand at 181.975 kb on + strand at 181.976 kb on - strand at 181.976 kb on - strand at 181.976 kb on - strand at 181.980 kb on + strand at 181.987 kb on + strand at 181.999 kb on + strand at 182.000 kb on - strand at 182.093 kb on + strand at 182.093 kb on + strand at 182.112 kb on + strand at 182.117 kb on + strand at 182.117 kb on + strand at 182.117 kb on + strand at 182.117 kb on + strand at 182.126 kb on + strand at 182.126 kb on + strand at 182.130 kb on + strand at 182.137 kb on + strand at 182.137 kb on + strand at 182.137 kb on + strand at 182.137 kb on + strand at 182.751 kb on + strand at 182.751 kb on + strand at 182.751 kb on + strand at 182.751 kb on + strand at 182.751 kb on + strand at 182.751 kb on + strand at 182.751 kb on + strand at 182.759 kb on + strand at 182.759 kb on + strand at 182.759 kb on + strand at 182.760 kb on - strand at 182.760 kb on - strand at 182.761 kb on + strand at 182.761 kb on + strand at 182.775 kb on + strand at 182.775 kb on + strand at 182.850 kb on - strand at 182.850 kb on - strand at 182.850 kb on - strand at 182.850 kb on - strand at 182.877 kb on - strand at 182.877 kb on - strand at 182.928 kb on + strand at 183.186 kb on - strand, within EX31_RS09885 at 183.186 kb on - strand, within EX31_RS09885 at 183.230 kb on + strand, within EX31_RS09885 at 183.277 kb on - strand, within EX31_RS09885 at 183.285 kb on + strand, within EX31_RS09885 at 183.285 kb on + strand, within EX31_RS09885 at 183.285 kb on + strand, within EX31_RS09885 at 183.285 kb on + strand, within EX31_RS09885 at 183.285 kb on + strand, within EX31_RS09885 at 183.286 kb on - strand, within EX31_RS09885 at 183.288 kb on - strand, within EX31_RS09885 at 183.426 kb on - strand, within EX31_RS09885 at 183.555 kb on + strand, within EX31_RS09885 at 183.585 kb on - strand, within EX31_RS09885 at 183.585 kb on - strand, within EX31_RS09885 at 183.609 kb on - strand, within EX31_RS09885 at 183.609 kb on - strand, within EX31_RS09885 at 183.741 kb on + strand, within EX31_RS09885 at 183.741 kb on + strand, within EX31_RS09885 at 183.742 kb on - strand, within EX31_RS09885 at 183.811 kb on - strand, within EX31_RS09885 at 183.882 kb on + strand, within EX31_RS09885 at 183.929 kb on - strand, within EX31_RS09885 at 184.059 kb on + strand, within EX31_RS09885 at 184.065 kb on - strand, within EX31_RS09885 at 184.241 kb on + strand, within EX31_RS09885 at 184.293 kb on + strand, within EX31_RS09885 at 184.293 kb on + strand, within EX31_RS09885 at 184.484 kb on - strand, within EX31_RS09885 at 184.526 kb on + strand, within EX31_RS09885 at 184.526 kb on + strand, within EX31_RS09885 at 184.526 kb on + strand, within EX31_RS09885 at 184.526 kb on + strand, within EX31_RS09885 at 184.527 kb on - strand, within EX31_RS09885 at 184.587 kb on + strand, within EX31_RS09885 at 184.587 kb on + strand, within EX31_RS09885 at 184.640 kb on + strand, within EX31_RS09885 at 184.861 kb on - strand, within EX31_RS09885 at 184.861 kb on - strand, within EX31_RS09885 at 184.922 kb on + strand, within EX31_RS09885 at 184.923 kb on - strand, within EX31_RS09885 at 184.943 kb on + strand, within EX31_RS09885 at 184.943 kb on + strand, within EX31_RS09885 at 184.969 kb on - strand, within EX31_RS09885 at 184.987 kb on + strand, within EX31_RS09885 at 185.153 kb on - strand, within EX31_RS09885 at 185.221 kb on + strand, within EX31_RS09885 at 185.262 kb on - strand at 185.314 kb on + strand at 185.356 kb on + strand at 185.436 kb on - strand at 185.438 kb on + strand at 185.438 kb on + strand at 185.439 kb on - strand at 185.442 kb on - strand at 185.491 kb on - strand at 185.499 kb on + strand at 185.499 kb on + strand at 185.499 kb on + strand at 185.499 kb on + strand at 185.500 kb on - strand at 185.500 kb on - strand at 185.500 kb on - strand at 185.500 kb on - strand at 185.537 kb on - strand at 185.549 kb on + strand at 185.550 kb on - strand at 185.550 kb on - strand at 185.550 kb on - strand at 185.550 kb on - strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.552 kb on - strand at 185.552 kb on - strand at 185.552 kb on - strand at 185.552 kb on - strand at 185.563 kb on + strand at 185.672 kb on - strand at 185.692 kb on + strand at 185.692 kb on + strand at 185.693 kb on - strand at 185.708 kb on + strand at 185.708 kb on + strand at 185.757 kb on - strand at 185.812 kb on + strand, within EX31_RS09890 at 185.813 kb on - strand, within EX31_RS09890 at 185.816 kb on + strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.818 kb on + strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.867 kb on - strand, within EX31_RS09890 at 185.867 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 186.004 kb on - strand, within EX31_RS09890 at 186.004 kb on - strand, within EX31_RS09890 at 186.074 kb on + strand, within EX31_RS09890 at 186.074 kb on + strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.126 kb on - strand, within EX31_RS09890 at 186.176 kb on - strand, within EX31_RS09890 at 186.203 kb on - strand, within EX31_RS09890 at 186.291 kb on - strand, within EX31_RS09890 at 186.330 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.380 kb on - strand, within EX31_RS09890 at 186.380 kb on - strand, within EX31_RS09890 at 186.400 kb on + strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.441 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 181,872 + +1.9 181,873 - -0.2 181,873 - +3.5 181,873 - -0.2 181,873 - +0.3 181,894 + -0.7 181,911 + -0.4 181,911 + +0.7 181,912 - +2.5 181,912 - +0.8 181,930 + +0.9 181,966 + -0.7 181,968 + -0.9 181,975 + -0.4 181,975 + -0.2 181,975 + +0.2 181,976 - -1.4 181,976 - +0.5 181,976 - -1.2 181,980 + +0.9 181,987 + -0.4 181,999 + -0.4 182,000 - +1.3 182,093 + +0.6 182,093 + +0.3 182,112 + +0.0 182,117 + -0.1 182,117 + -0.2 182,117 + +0.5 182,117 + -0.5 182,126 + +0.3 182,126 + -0.4 182,130 + +1.7 182,137 + +1.1 182,137 + +0.0 182,137 + -0.1 182,137 + -0.9 182,751 + +0.2 182,751 + -2.5 182,751 + -0.1 182,751 + +1.3 182,751 + +1.5 182,751 + +0.9 182,751 + +1.3 182,759 + +2.3 182,759 + +1.2 182,759 + +2.5 182,760 - +0.8 182,760 - +0.8 182,761 + -0.2 182,761 + +0.1 182,775 + -0.8 182,775 + +2.5 182,850 - +0.3 182,850 - +1.6 182,850 - +0.8 182,850 - -0.1 182,877 - +0.1 182,877 - -0.9 182,928 + +0.4 183,186 - EX31_RS09885 0.13 +1.4 183,186 - EX31_RS09885 0.13 -0.2 183,230 + EX31_RS09885 0.15 +0.3 183,277 - EX31_RS09885 0.17 +0.9 183,285 + EX31_RS09885 0.17 +0.8 183,285 + EX31_RS09885 0.17 +0.4 183,285 + EX31_RS09885 0.17 +2.5 183,285 + EX31_RS09885 0.17 -0.4 183,285 + EX31_RS09885 0.17 -1.0 183,286 - EX31_RS09885 0.17 +0.4 183,288 - EX31_RS09885 0.17 +0.2 183,426 - EX31_RS09885 0.22 +1.0 183,555 + EX31_RS09885 0.27 -1.4 183,585 - EX31_RS09885 0.28 +0.7 183,585 - EX31_RS09885 0.28 +0.6 183,609 - EX31_RS09885 0.29 -0.5 183,609 - EX31_RS09885 0.29 -1.3 183,741 + EX31_RS09885 0.34 -0.4 183,741 + EX31_RS09885 0.34 +0.1 183,742 - EX31_RS09885 0.34 +0.5 183,811 - EX31_RS09885 0.37 +0.9 183,882 + EX31_RS09885 0.39 +1.4 183,929 - EX31_RS09885 0.41 +1.3 184,059 + EX31_RS09885 0.46 +2.5 184,065 - EX31_RS09885 0.46 +0.7 184,241 + EX31_RS09885 0.53 +1.0 184,293 + EX31_RS09885 0.55 +0.3 184,293 + EX31_RS09885 0.55 +0.5 184,484 - EX31_RS09885 0.62 +1.3 184,526 + EX31_RS09885 0.64 -2.3 184,526 + EX31_RS09885 0.64 +0.1 184,526 + EX31_RS09885 0.64 -0.0 184,526 + EX31_RS09885 0.64 -0.1 184,527 - EX31_RS09885 0.64 +0.1 184,587 + EX31_RS09885 0.66 +0.7 184,587 + EX31_RS09885 0.66 -0.9 184,640 + EX31_RS09885 0.68 -0.1 184,861 - EX31_RS09885 0.76 +0.9 184,861 - EX31_RS09885 0.76 +0.3 184,922 + EX31_RS09885 0.78 +0.1 184,923 - EX31_RS09885 0.78 +0.1 184,943 + EX31_RS09885 0.79 +0.2 184,943 + EX31_RS09885 0.79 +1.3 184,969 - EX31_RS09885 0.80 +1.7 184,987 + EX31_RS09885 0.81 -0.2 185,153 - EX31_RS09885 0.87 -1.1 185,221 + EX31_RS09885 0.90 +1.8 185,262 - +0.9 185,314 + +0.5 185,356 + +0.1 185,436 - +1.8 185,438 + +1.1 185,438 + +2.9 185,439 - -0.1 185,442 - +0.1 185,491 - +0.7 185,499 + +0.9 185,499 + +1.5 185,499 + +1.3 185,499 + +0.9 185,500 - -0.4 185,500 - -0.7 185,500 - -1.7 185,500 - +0.8 185,537 - +0.5 185,549 + +0.6 185,550 - -0.6 185,550 - +0.6 185,550 - +1.7 185,550 - +0.3 185,551 + +0.9 185,551 + +0.3 185,551 + +0.1 185,551 + +2.9 185,551 + -0.1 185,552 - +0.8 185,552 - +1.9 185,552 - +1.7 185,552 - -0.7 185,563 + +1.0 185,672 - +3.7 185,692 + +0.7 185,692 + -0.4 185,693 - +0.7 185,708 + +0.6 185,708 + -0.1 185,757 - -3.5 185,812 + EX31_RS09890 0.12 -1.7 185,813 - EX31_RS09890 0.13 -1.5 185,816 + EX31_RS09890 0.13 -2.7 185,817 - EX31_RS09890 0.13 -1.5 185,817 - EX31_RS09890 0.13 -0.9 185,817 - EX31_RS09890 0.13 -2.6 185,817 - EX31_RS09890 0.13 -1.2 185,817 - EX31_RS09890 0.13 -2.0 185,817 - EX31_RS09890 0.13 -3.1 185,817 - EX31_RS09890 0.13 -2.3 185,817 - EX31_RS09890 0.13 +0.9 185,817 - EX31_RS09890 0.13 +0.1 185,817 - EX31_RS09890 0.13 -3.2 185,817 - EX31_RS09890 0.13 -1.9 185,817 - EX31_RS09890 0.13 -1.2 185,817 - EX31_RS09890 0.13 -0.7 185,817 - EX31_RS09890 0.13 -1.5 185,817 - EX31_RS09890 0.13 -0.4 185,818 + EX31_RS09890 0.13 -1.2 185,819 - EX31_RS09890 0.13 +1.9 185,819 - EX31_RS09890 0.13 -3.5 185,819 - EX31_RS09890 0.13 -1.2 185,819 - EX31_RS09890 0.13 -1.8 185,819 - EX31_RS09890 0.13 -3.4 185,819 - EX31_RS09890 0.13 -3.6 185,819 - EX31_RS09890 0.13 -2.7 185,819 - EX31_RS09890 0.13 -2.0 185,819 - EX31_RS09890 0.13 -0.1 185,819 - EX31_RS09890 0.13 -2.3 185,819 - EX31_RS09890 0.13 -2.3 185,819 - EX31_RS09890 0.13 -3.3 185,819 - EX31_RS09890 0.13 -1.6 185,819 - EX31_RS09890 0.13 -1.5 185,819 - EX31_RS09890 0.13 -2.3 185,819 - EX31_RS09890 0.13 -0.9 185,819 - EX31_RS09890 0.13 -2.2 185,819 - EX31_RS09890 0.13 -2.2 185,819 - EX31_RS09890 0.13 -2.2 185,819 - EX31_RS09890 0.13 -0.2 185,819 - EX31_RS09890 0.13 -2.2 185,867 - EX31_RS09890 0.18 -1.7 185,867 - EX31_RS09890 0.18 -1.2 185,969 - EX31_RS09890 0.29 -1.8 185,969 - EX31_RS09890 0.29 -1.1 185,969 - EX31_RS09890 0.29 -3.0 185,969 - EX31_RS09890 0.29 -2.8 186,004 - EX31_RS09890 0.32 -1.8 186,004 - EX31_RS09890 0.32 -2.5 186,074 + EX31_RS09890 0.39 -0.4 186,074 + EX31_RS09890 0.39 +0.3 186,075 - EX31_RS09890 0.39 -1.2 186,075 - EX31_RS09890 0.39 -1.0 186,075 - EX31_RS09890 0.39 -2.5 186,077 - EX31_RS09890 0.40 -1.9 186,077 - EX31_RS09890 0.40 -1.2 186,077 - EX31_RS09890 0.40 +0.3 186,126 - EX31_RS09890 0.45 -3.2 186,176 - EX31_RS09890 0.50 +0.3 186,203 - EX31_RS09890 0.53 -2.5 186,291 - EX31_RS09890 0.62 -1.4 186,330 - EX31_RS09890 0.66 -2.2 186,363 - EX31_RS09890 0.69 -0.4 186,363 - EX31_RS09890 0.69 +0.9 186,363 - EX31_RS09890 0.69 -1.5 186,380 - EX31_RS09890 0.71 -0.8 186,380 - EX31_RS09890 0.71 -2.5 186,400 + EX31_RS09890 0.73 +1.9 186,405 - EX31_RS09890 0.73 +0.2 186,405 - EX31_RS09890 0.73 +0.3 186,405 - EX31_RS09890 0.73 -1.0 186,405 - EX31_RS09890 0.73 -0.4 186,405 - EX31_RS09890 0.73 -3.3 186,405 - EX31_RS09890 0.73 -2.7 186,405 - EX31_RS09890 0.73 -1.6 186,441 - EX31_RS09890 0.77 -2.9 186,448 - EX31_RS09890 0.78 -1.4 186,448 - EX31_RS09890 0.78 -1.2 186,448 - EX31_RS09890 0.78 -0.7 186,448 - EX31_RS09890 0.78 -1.5 186,448 - EX31_RS09890 0.78 -0.6
Or see this region's nucleotide sequence