Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03290 and EX31_RS03295 are separated by 143 nucleotides EX31_RS03295 and EX31_RS03300 overlap by 1 nucleotides EX31_RS03300 and EX31_RS03305 are separated by 135 nucleotides
EX31_RS03290: EX31_RS03290 - aspartate 1-decarboxylase, at 149,023 to 149,403
_RS03290
EX31_RS03295: EX31_RS03295 - ABC transporter permease, at 149,547 to 150,317
_RS03295
EX31_RS03300: EX31_RS03300 - ABC transporter ATP-binding protein, at 150,317 to 151,240
_RS03300
EX31_RS03305: EX31_RS03305 - carbonate dehydratase, at 151,376 to 152,032
_RS03305
Position (kb) 150
151
152 Strain fitness (log2 ratio) -2
-1
0
1
2 at 149.451 kb on + strand at 149.466 kb on + strand at 149.466 kb on + strand at 149.467 kb on - strand at 149.539 kb on + strand at 149.609 kb on + strand at 149.609 kb on + strand at 149.610 kb on - strand at 149.634 kb on - strand, within EX31_RS03295 at 149.693 kb on - strand, within EX31_RS03295 at 149.693 kb on - strand, within EX31_RS03295 at 149.693 kb on - strand, within EX31_RS03295 at 149.693 kb on - strand, within EX31_RS03295 at 149.712 kb on - strand, within EX31_RS03295 at 149.771 kb on - strand, within EX31_RS03295 at 149.877 kb on + strand, within EX31_RS03295 at 149.993 kb on - strand, within EX31_RS03295 at 150.044 kb on + strand, within EX31_RS03295 at 150.120 kb on - strand, within EX31_RS03295 at 150.135 kb on - strand, within EX31_RS03295 at 150.145 kb on + strand, within EX31_RS03295 at 150.145 kb on + strand, within EX31_RS03295 at 150.145 kb on + strand, within EX31_RS03295 at 150.146 kb on - strand, within EX31_RS03295 at 150.146 kb on - strand, within EX31_RS03295 at 150.146 kb on - strand, within EX31_RS03295 at 150.146 kb on - strand, within EX31_RS03295 at 150.206 kb on - strand, within EX31_RS03295 at 150.206 kb on - strand, within EX31_RS03295 at 150.206 kb on - strand, within EX31_RS03295 at 150.208 kb on - strand, within EX31_RS03295 at 150.208 kb on - strand, within EX31_RS03295 at 150.208 kb on - strand, within EX31_RS03295 at 150.208 kb on - strand, within EX31_RS03295 at 150.285 kb on - strand at 150.285 kb on - strand at 150.285 kb on - strand at 150.285 kb on - strand at 150.305 kb on - strand at 150.305 kb on - strand at 150.318 kb on + strand at 150.319 kb on - strand at 150.319 kb on - strand at 150.319 kb on - strand at 150.319 kb on - strand at 150.319 kb on - strand at 150.319 kb on - strand at 150.382 kb on + strand at 150.383 kb on - strand at 150.383 kb on - strand at 150.383 kb on - strand at 150.383 kb on - strand at 150.389 kb on - strand at 150.395 kb on + strand at 150.534 kb on - strand, within EX31_RS03300 at 150.617 kb on - strand, within EX31_RS03300 at 150.617 kb on - strand, within EX31_RS03300 at 150.619 kb on + strand, within EX31_RS03300 at 150.620 kb on - strand, within EX31_RS03300 at 150.658 kb on - strand, within EX31_RS03300 at 150.658 kb on - strand, within EX31_RS03300 at 150.729 kb on - strand, within EX31_RS03300 at 150.729 kb on - strand, within EX31_RS03300 at 150.729 kb on - strand, within EX31_RS03300 at 150.927 kb on + strand, within EX31_RS03300 at 150.940 kb on + strand, within EX31_RS03300 at 150.980 kb on - strand, within EX31_RS03300 at 151.024 kb on + strand, within EX31_RS03300 at 151.187 kb on + strand at 151.187 kb on + strand at 151.188 kb on - strand at 151.188 kb on - strand at 151.201 kb on - strand at 151.233 kb on + strand at 151.233 kb on + strand at 151.233 kb on + strand at 151.233 kb on + strand at 151.233 kb on + strand at 151.233 kb on + strand at 151.234 kb on - strand at 151.234 kb on - strand at 151.234 kb on - strand at 151.234 kb on - strand at 151.234 kb on - strand at 151.252 kb on - strand at 151.275 kb on + strand at 151.275 kb on + strand at 151.275 kb on + strand at 151.275 kb on + strand at 151.275 kb on + strand at 151.275 kb on + strand at 151.276 kb on - strand at 151.276 kb on - strand at 151.276 kb on - strand at 151.276 kb on - strand at 151.329 kb on + strand at 151.329 kb on + strand at 151.329 kb on + strand at 151.329 kb on + strand at 151.330 kb on - strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.331 kb on + strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.332 kb on - strand at 151.334 kb on + strand at 151.334 kb on + strand at 151.334 kb on + strand at 151.334 kb on + strand at 151.335 kb on - strand at 151.335 kb on - strand at 151.335 kb on - strand at 151.335 kb on - strand at 151.335 kb on - strand at 151.335 kb on - strand at 151.355 kb on + strand at 151.355 kb on + strand at 151.355 kb on + strand at 151.365 kb on + strand at 151.366 kb on - strand at 151.366 kb on - strand at 151.366 kb on - strand at 151.366 kb on - strand at 151.368 kb on + strand at 151.368 kb on + strand at 151.368 kb on + strand at 151.386 kb on + strand at 151.386 kb on + strand at 151.386 kb on + strand at 151.386 kb on + strand at 151.386 kb on + strand at 151.386 kb on - strand at 151.387 kb on - strand at 151.387 kb on - strand at 151.387 kb on - strand at 151.387 kb on - strand at 151.387 kb on - strand at 151.387 kb on - strand at 151.600 kb on + strand, within EX31_RS03305 at 151.600 kb on + strand, within EX31_RS03305 at 151.600 kb on + strand, within EX31_RS03305 at 151.600 kb on + strand, within EX31_RS03305 at 151.601 kb on - strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.622 kb on + strand, within EX31_RS03305 at 151.623 kb on - strand, within EX31_RS03305 at 151.623 kb on - strand, within EX31_RS03305 at 151.658 kb on - strand, within EX31_RS03305 at 151.658 kb on - strand, within EX31_RS03305 at 151.671 kb on - strand, within EX31_RS03305 at 151.726 kb on + strand, within EX31_RS03305 at 151.726 kb on + strand, within EX31_RS03305 at 151.727 kb on - strand, within EX31_RS03305 at 151.727 kb on - strand, within EX31_RS03305 at 151.758 kb on - strand, within EX31_RS03305 at 151.812 kb on + strand, within EX31_RS03305 at 151.812 kb on + strand, within EX31_RS03305 at 151.812 kb on + strand, within EX31_RS03305 at 151.812 kb on + strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.813 kb on - strand, within EX31_RS03305 at 151.842 kb on - strand, within EX31_RS03305 at 151.917 kb on - strand, within EX31_RS03305 at 152.049 kb on - strand at 152.103 kb on - strand at 152.105 kb on + strand at 152.126 kb on - strand at 152.168 kb on + strand at 152.168 kb on + strand at 152.168 kb on + strand at 152.169 kb on - strand at 152.169 kb on - strand at 152.169 kb on - strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.170 kb on + strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand at 152.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 149,451 + +0.9 149,466 + -0.5 149,466 + +0.2 149,467 - +0.1 149,539 + +0.5 149,609 + -1.4 149,609 + -0.9 149,610 - -1.0 149,634 - EX31_RS03295 0.11 -1.1 149,693 - EX31_RS03295 0.19 -0.7 149,693 - EX31_RS03295 0.19 -1.7 149,693 - EX31_RS03295 0.19 +0.2 149,693 - EX31_RS03295 0.19 +1.4 149,712 - EX31_RS03295 0.21 +1.3 149,771 - EX31_RS03295 0.29 +0.2 149,877 + EX31_RS03295 0.43 +0.2 149,993 - EX31_RS03295 0.58 +0.9 150,044 + EX31_RS03295 0.64 +0.2 150,120 - EX31_RS03295 0.74 +0.2 150,135 - EX31_RS03295 0.76 -0.1 150,145 + EX31_RS03295 0.78 +1.2 150,145 + EX31_RS03295 0.78 +1.8 150,145 + EX31_RS03295 0.78 -0.1 150,146 - EX31_RS03295 0.78 +1.1 150,146 - EX31_RS03295 0.78 +0.8 150,146 - EX31_RS03295 0.78 -0.1 150,146 - EX31_RS03295 0.78 -1.4 150,206 - EX31_RS03295 0.85 -0.2 150,206 - EX31_RS03295 0.85 -0.7 150,206 - EX31_RS03295 0.85 +0.6 150,208 - EX31_RS03295 0.86 +0.1 150,208 - EX31_RS03295 0.86 +0.4 150,208 - EX31_RS03295 0.86 +1.5 150,208 - EX31_RS03295 0.86 -0.6 150,285 - -1.6 150,285 - -1.0 150,285 - -1.2 150,285 - +0.1 150,305 - -0.1 150,305 - +0.1 150,318 + +0.6 150,319 - -1.0 150,319 - -1.0 150,319 - -0.4 150,319 - -1.3 150,319 - +0.3 150,319 - -0.0 150,382 + -0.3 150,383 - +0.2 150,383 - -0.3 150,383 - +0.2 150,383 - +0.1 150,389 - +0.1 150,395 + +0.4 150,534 - EX31_RS03300 0.23 +0.6 150,617 - EX31_RS03300 0.32 -0.0 150,617 - EX31_RS03300 0.32 -1.6 150,619 + EX31_RS03300 0.33 +0.4 150,620 - EX31_RS03300 0.33 +0.4 150,658 - EX31_RS03300 0.37 +0.2 150,658 - EX31_RS03300 0.37 +0.1 150,729 - EX31_RS03300 0.45 +0.5 150,729 - EX31_RS03300 0.45 -0.3 150,729 - EX31_RS03300 0.45 +1.8 150,927 + EX31_RS03300 0.66 +0.5 150,940 + EX31_RS03300 0.67 +1.2 150,980 - EX31_RS03300 0.72 -0.4 151,024 + EX31_RS03300 0.77 -0.3 151,187 + -0.2 151,187 + -0.2 151,188 - -0.6 151,188 - +0.3 151,201 - -0.6 151,233 + -2.0 151,233 + +0.1 151,233 + +0.1 151,233 + +0.0 151,233 + +0.7 151,233 + +0.3 151,234 - +0.1 151,234 - -0.1 151,234 - +0.7 151,234 - -0.4 151,234 - +0.2 151,252 - -0.3 151,275 + -0.8 151,275 + +0.4 151,275 + +0.8 151,275 + -0.0 151,275 + -1.7 151,275 + -1.6 151,276 - -0.4 151,276 - -0.6 151,276 - -0.5 151,276 - -0.1 151,329 + -0.8 151,329 + +0.9 151,329 + -0.8 151,329 + -0.2 151,330 - +0.6 151,331 + +0.5 151,331 + -0.5 151,331 + +0.6 151,331 + +0.2 151,331 + -0.3 151,331 + -1.2 151,331 + +0.2 151,331 + +0.4 151,332 - -1.0 151,332 - -1.2 151,332 - -0.2 151,332 - +0.4 151,332 - -0.1 151,332 - +0.6 151,332 - +0.0 151,332 - -1.0 151,332 - +0.7 151,332 - +0.6 151,332 - -0.2 151,334 + -0.2 151,334 + +0.2 151,334 + +0.3 151,334 + -0.7 151,335 - -0.8 151,335 - -0.5 151,335 - -0.5 151,335 - -2.0 151,335 - -0.2 151,335 - -0.2 151,355 + -0.5 151,355 + -0.9 151,355 + -0.2 151,365 + +0.4 151,366 - +1.0 151,366 - -0.8 151,366 - +0.3 151,366 - +1.2 151,368 + -0.7 151,368 + +0.2 151,368 + -1.7 151,386 + +0.2 151,386 + +2.2 151,386 + +1.1 151,386 + +0.2 151,386 + -0.4 151,386 - +0.3 151,387 - -0.4 151,387 - +0.5 151,387 - -0.9 151,387 - -0.4 151,387 - -1.2 151,387 - -1.3 151,600 + EX31_RS03305 0.34 +0.1 151,600 + EX31_RS03305 0.34 +0.7 151,600 + EX31_RS03305 0.34 +2.2 151,600 + EX31_RS03305 0.34 -1.7 151,601 - EX31_RS03305 0.34 -0.0 151,622 + EX31_RS03305 0.37 -0.5 151,622 + EX31_RS03305 0.37 +2.0 151,622 + EX31_RS03305 0.37 -0.5 151,622 + EX31_RS03305 0.37 -0.6 151,622 + EX31_RS03305 0.37 +0.9 151,622 + EX31_RS03305 0.37 -0.5 151,622 + EX31_RS03305 0.37 +1.2 151,622 + EX31_RS03305 0.37 +0.4 151,622 + EX31_RS03305 0.37 -0.7 151,622 + EX31_RS03305 0.37 -0.4 151,622 + EX31_RS03305 0.37 -0.0 151,623 - EX31_RS03305 0.38 +0.0 151,623 - EX31_RS03305 0.38 -0.9 151,658 - EX31_RS03305 0.43 -1.0 151,658 - EX31_RS03305 0.43 +1.2 151,671 - EX31_RS03305 0.45 +1.0 151,726 + EX31_RS03305 0.53 +0.0 151,726 + EX31_RS03305 0.53 +0.2 151,727 - EX31_RS03305 0.53 +1.3 151,727 - EX31_RS03305 0.53 +0.0 151,758 - EX31_RS03305 0.58 +1.6 151,812 + EX31_RS03305 0.66 -0.6 151,812 + EX31_RS03305 0.66 -0.3 151,812 + EX31_RS03305 0.66 -0.3 151,812 + EX31_RS03305 0.66 -0.1 151,813 - EX31_RS03305 0.67 -0.1 151,813 - EX31_RS03305 0.67 +0.3 151,813 - EX31_RS03305 0.67 -1.5 151,813 - EX31_RS03305 0.67 +0.0 151,813 - EX31_RS03305 0.67 +0.5 151,813 - EX31_RS03305 0.67 +0.0 151,813 - EX31_RS03305 0.67 +0.8 151,813 - EX31_RS03305 0.67 +0.5 151,813 - EX31_RS03305 0.67 -1.9 151,842 - EX31_RS03305 0.71 -0.4 151,917 - EX31_RS03305 0.82 -0.0 152,049 - +1.0 152,103 - -2.3 152,105 + +0.8 152,126 - -0.2 152,168 + -0.4 152,168 + -0.3 152,168 + +1.2 152,169 - -1.0 152,169 - -0.7 152,169 - -0.9 152,170 + +0.2 152,170 + -0.6 152,170 + +1.0 152,170 + +0.2 152,170 + +0.7 152,170 + -0.7 152,170 + -0.3 152,170 + +1.2 152,170 + +0.5 152,170 + +0.3 152,170 + -0.4 152,170 + +0.8 152,171 - -0.3 152,171 - -0.2 152,171 - -0.5 152,171 - -1.2 152,171 - -0.3 152,171 - -0.9 152,171 - -0.6 152,171 - -0.1 152,171 - +0.3 152,171 - +0.6 152,171 - -0.4
Or see this region's nucleotide sequence