Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00540 and EX31_RS00545 are separated by 51 nucleotides EX31_RS00545 and EX31_RS00550 are separated by 10 nucleotides EX31_RS00550 and EX31_RS00555 are separated by 153 nucleotides EX31_RS00555 and EX31_RS00560 are separated by 3 nucleotides
EX31_RS00540: EX31_RS00540 - pentapeptide MXKDX repeat protein, at 116,218 to 116,484
_RS00540
EX31_RS00545: EX31_RS00545 - molybdopterin-dependent oxidoreductase, at 116,536 to 117,339
_RS00545
EX31_RS00550: EX31_RS00550 - cytochrome b/b6 domain-containing protein, at 117,350 to 117,976
_RS00550
EX31_RS00555: EX31_RS00555 - response regulator transcription factor, at 118,130 to 118,852
_RS00555
EX31_RS00560: EX31_RS00560 - HAMP domain-containing histidine kinase, at 118,856 to 120,322
_RS00560
Position (kb)
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 116.350 kb on - strand, within EX31_RS00540 at 116.379 kb on + strand, within EX31_RS00540 at 116.379 kb on + strand, within EX31_RS00540 at 116.379 kb on + strand, within EX31_RS00540 at 116.420 kb on + strand, within EX31_RS00540 at 116.468 kb on - strand at 116.474 kb on - strand at 116.488 kb on - strand at 116.499 kb on + strand at 116.501 kb on + strand at 116.501 kb on + strand at 116.501 kb on + strand at 116.501 kb on + strand at 116.502 kb on - strand at 116.502 kb on - strand at 116.512 kb on + strand at 116.512 kb on + strand at 116.513 kb on - strand at 116.513 kb on - strand at 116.790 kb on - strand, within EX31_RS00545 at 116.919 kb on - strand, within EX31_RS00545 at 117.062 kb on + strand, within EX31_RS00545 at 117.062 kb on + strand, within EX31_RS00545 at 117.063 kb on - strand, within EX31_RS00545 at 117.215 kb on - strand, within EX31_RS00545 at 117.252 kb on + strand, within EX31_RS00545 at 117.457 kb on - strand, within EX31_RS00550 at 117.570 kb on + strand, within EX31_RS00550 at 117.571 kb on - strand, within EX31_RS00550 at 117.594 kb on + strand, within EX31_RS00550 at 117.595 kb on - strand, within EX31_RS00550 at 117.595 kb on - strand, within EX31_RS00550 at 117.603 kb on - strand, within EX31_RS00550 at 117.752 kb on - strand, within EX31_RS00550 at 117.772 kb on + strand, within EX31_RS00550 at 117.772 kb on + strand, within EX31_RS00550 at 117.843 kb on + strand, within EX31_RS00550 at 117.843 kb on + strand, within EX31_RS00550 at 117.844 kb on - strand, within EX31_RS00550 at 117.868 kb on + strand, within EX31_RS00550 at 117.868 kb on + strand, within EX31_RS00550 at 117.869 kb on - strand, within EX31_RS00550 at 117.869 kb on - strand, within EX31_RS00550 at 117.968 kb on + strand at 117.968 kb on + strand at 117.968 kb on + strand at 117.968 kb on + strand at 117.969 kb on - strand at 117.976 kb on + strand at 118.013 kb on + strand at 118.013 kb on + strand at 118.056 kb on - strand at 118.088 kb on + strand at 118.089 kb on - strand at 118.135 kb on + strand at 118.135 kb on + strand at 118.222 kb on + strand, within EX31_RS00555 at 118.357 kb on + strand, within EX31_RS00555 at 118.357 kb on + strand, within EX31_RS00555 at 118.358 kb on - strand, within EX31_RS00555 at 118.369 kb on + strand, within EX31_RS00555 at 118.369 kb on + strand, within EX31_RS00555 at 118.369 kb on + strand, within EX31_RS00555 at 118.370 kb on - strand, within EX31_RS00555 at 118.370 kb on - strand, within EX31_RS00555 at 118.370 kb on - strand, within EX31_RS00555 at 118.433 kb on + strand, within EX31_RS00555 at 118.453 kb on + strand, within EX31_RS00555 at 118.453 kb on + strand, within EX31_RS00555 at 118.514 kb on - strand, within EX31_RS00555 at 118.635 kb on - strand, within EX31_RS00555 at 118.684 kb on - strand, within EX31_RS00555 at 118.715 kb on + strand, within EX31_RS00555 at 118.716 kb on - strand, within EX31_RS00555 at 118.723 kb on + strand, within EX31_RS00555 at 118.739 kb on - strand, within EX31_RS00555 at 118.761 kb on + strand, within EX31_RS00555 at 118.762 kb on - strand, within EX31_RS00555 at 118.850 kb on + strand at 118.850 kb on + strand at 118.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 116,350 - EX31_RS00540 0.49 -0.1 116,379 + EX31_RS00540 0.60 +1.1 116,379 + EX31_RS00540 0.60 -0.4 116,379 + EX31_RS00540 0.60 +0.5 116,420 + EX31_RS00540 0.76 +1.0 116,468 - -1.9 116,474 - -1.4 116,488 - -0.1 116,499 + +0.2 116,501 + +0.4 116,501 + +0.3 116,501 + +0.2 116,501 + -0.2 116,502 - +1.4 116,502 - +1.1 116,512 + +0.9 116,512 + -2.0 116,513 - -1.5 116,513 - -2.6 116,790 - EX31_RS00545 0.32 +1.1 116,919 - EX31_RS00545 0.48 +1.6 117,062 + EX31_RS00545 0.65 +0.0 117,062 + EX31_RS00545 0.65 +0.5 117,063 - EX31_RS00545 0.66 +1.1 117,215 - EX31_RS00545 0.84 +0.1 117,252 + EX31_RS00545 0.89 +0.6 117,457 - EX31_RS00550 0.17 +2.1 117,570 + EX31_RS00550 0.35 +0.0 117,571 - EX31_RS00550 0.35 +0.7 117,594 + EX31_RS00550 0.39 +0.4 117,595 - EX31_RS00550 0.39 -2.7 117,595 - EX31_RS00550 0.39 -0.3 117,603 - EX31_RS00550 0.40 +1.2 117,752 - EX31_RS00550 0.64 -0.4 117,772 + EX31_RS00550 0.67 +0.1 117,772 + EX31_RS00550 0.67 +0.7 117,843 + EX31_RS00550 0.79 +0.5 117,843 + EX31_RS00550 0.79 +1.6 117,844 - EX31_RS00550 0.79 +0.8 117,868 + EX31_RS00550 0.83 -1.4 117,868 + EX31_RS00550 0.83 +0.9 117,869 - EX31_RS00550 0.83 +0.5 117,869 - EX31_RS00550 0.83 -0.4 117,968 + +2.1 117,968 + -1.2 117,968 + +0.5 117,968 + +1.5 117,969 - -0.5 117,976 + +1.3 118,013 + +1.6 118,013 + -0.4 118,056 - -1.0 118,088 + -1.1 118,089 - +1.5 118,135 + +0.7 118,135 + -1.2 118,222 + EX31_RS00555 0.13 -1.8 118,357 + EX31_RS00555 0.31 +0.3 118,357 + EX31_RS00555 0.31 +0.8 118,358 - EX31_RS00555 0.32 -0.3 118,369 + EX31_RS00555 0.33 +0.1 118,369 + EX31_RS00555 0.33 +0.5 118,369 + EX31_RS00555 0.33 -0.4 118,370 - EX31_RS00555 0.33 +1.7 118,370 - EX31_RS00555 0.33 -0.1 118,370 - EX31_RS00555 0.33 -1.4 118,433 + EX31_RS00555 0.42 +1.3 118,453 + EX31_RS00555 0.45 -0.7 118,453 + EX31_RS00555 0.45 -0.3 118,514 - EX31_RS00555 0.53 +0.6 118,635 - EX31_RS00555 0.70 -0.8 118,684 - EX31_RS00555 0.77 +0.9 118,715 + EX31_RS00555 0.81 +0.4 118,716 - EX31_RS00555 0.81 -0.5 118,723 + EX31_RS00555 0.82 -0.5 118,739 - EX31_RS00555 0.84 +1.1 118,761 + EX31_RS00555 0.87 +0.7 118,762 - EX31_RS00555 0.87 -0.1 118,850 + -0.8 118,850 + +0.5 118,851 - -0.5
Or see this region's nucleotide sequence