Strain Fitness in Rahnella sp. WP5 around EX31_RS00550

Experiment: Ammonium chloride (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS00540 and EX31_RS00545 are separated by 51 nucleotidesEX31_RS00545 and EX31_RS00550 are separated by 10 nucleotidesEX31_RS00550 and EX31_RS00555 are separated by 153 nucleotidesEX31_RS00555 and EX31_RS00560 are separated by 3 nucleotides EX31_RS00540: EX31_RS00540 - pentapeptide MXKDX repeat protein, at 116,218 to 116,484 _RS00540 EX31_RS00545: EX31_RS00545 - molybdopterin-dependent oxidoreductase, at 116,536 to 117,339 _RS00545 EX31_RS00550: EX31_RS00550 - cytochrome b/b6 domain-containing protein, at 117,350 to 117,976 _RS00550 EX31_RS00555: EX31_RS00555 - response regulator transcription factor, at 118,130 to 118,852 _RS00555 EX31_RS00560: EX31_RS00560 - HAMP domain-containing histidine kinase, at 118,856 to 120,322 _RS00560 Position (kb) 117 118Strain fitness (log2 ratio) -2 -1 0 1 2at 116.350 kb on - strand, within EX31_RS00540at 116.379 kb on + strand, within EX31_RS00540at 116.379 kb on + strand, within EX31_RS00540at 116.379 kb on + strand, within EX31_RS00540at 116.420 kb on + strand, within EX31_RS00540at 116.468 kb on - strandat 116.474 kb on - strandat 116.488 kb on - strandat 116.499 kb on + strandat 116.501 kb on + strandat 116.501 kb on + strandat 116.501 kb on + strandat 116.501 kb on + strandat 116.502 kb on - strandat 116.502 kb on - strandat 116.512 kb on + strandat 116.512 kb on + strandat 116.513 kb on - strandat 116.513 kb on - strandat 116.790 kb on - strand, within EX31_RS00545at 116.919 kb on - strand, within EX31_RS00545at 117.062 kb on + strand, within EX31_RS00545at 117.062 kb on + strand, within EX31_RS00545at 117.063 kb on - strand, within EX31_RS00545at 117.215 kb on - strand, within EX31_RS00545at 117.252 kb on + strand, within EX31_RS00545at 117.457 kb on - strand, within EX31_RS00550at 117.570 kb on + strand, within EX31_RS00550at 117.571 kb on - strand, within EX31_RS00550at 117.594 kb on + strand, within EX31_RS00550at 117.595 kb on - strand, within EX31_RS00550at 117.595 kb on - strand, within EX31_RS00550at 117.603 kb on - strand, within EX31_RS00550at 117.752 kb on - strand, within EX31_RS00550at 117.772 kb on + strand, within EX31_RS00550at 117.772 kb on + strand, within EX31_RS00550at 117.843 kb on + strand, within EX31_RS00550at 117.843 kb on + strand, within EX31_RS00550at 117.844 kb on - strand, within EX31_RS00550at 117.868 kb on + strand, within EX31_RS00550at 117.868 kb on + strand, within EX31_RS00550at 117.869 kb on - strand, within EX31_RS00550at 117.869 kb on - strand, within EX31_RS00550at 117.968 kb on + strandat 117.968 kb on + strandat 117.968 kb on + strandat 117.968 kb on + strandat 117.969 kb on - strandat 117.976 kb on + strandat 118.013 kb on + strandat 118.013 kb on + strandat 118.056 kb on - strandat 118.088 kb on + strandat 118.089 kb on - strandat 118.135 kb on + strandat 118.135 kb on + strandat 118.222 kb on + strand, within EX31_RS00555at 118.357 kb on + strand, within EX31_RS00555at 118.357 kb on + strand, within EX31_RS00555at 118.358 kb on - strand, within EX31_RS00555at 118.369 kb on + strand, within EX31_RS00555at 118.369 kb on + strand, within EX31_RS00555at 118.369 kb on + strand, within EX31_RS00555at 118.370 kb on - strand, within EX31_RS00555at 118.370 kb on - strand, within EX31_RS00555at 118.370 kb on - strand, within EX31_RS00555at 118.433 kb on + strand, within EX31_RS00555at 118.453 kb on + strand, within EX31_RS00555at 118.453 kb on + strand, within EX31_RS00555at 118.514 kb on - strand, within EX31_RS00555at 118.635 kb on - strand, within EX31_RS00555at 118.684 kb on - strand, within EX31_RS00555at 118.715 kb on + strand, within EX31_RS00555at 118.716 kb on - strand, within EX31_RS00555at 118.723 kb on + strand, within EX31_RS00555at 118.739 kb on - strand, within EX31_RS00555at 118.761 kb on + strand, within EX31_RS00555at 118.762 kb on - strand, within EX31_RS00555at 118.850 kb on + strandat 118.850 kb on + strandat 118.851 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking
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116,350 - EX31_RS00540 0.49 -0.1
116,379 + EX31_RS00540 0.60 +1.1
116,379 + EX31_RS00540 0.60 -0.4
116,379 + EX31_RS00540 0.60 +0.5
116,420 + EX31_RS00540 0.76 +1.0
116,468 - -1.9
116,474 - -1.4
116,488 - -0.1
116,499 + +0.2
116,501 + +0.4
116,501 + +0.3
116,501 + +0.2
116,501 + -0.2
116,502 - +1.4
116,502 - +1.1
116,512 + +0.9
116,512 + -2.0
116,513 - -1.5
116,513 - -2.6
116,790 - EX31_RS00545 0.32 +1.1
116,919 - EX31_RS00545 0.48 +1.6
117,062 + EX31_RS00545 0.65 +0.0
117,062 + EX31_RS00545 0.65 +0.5
117,063 - EX31_RS00545 0.66 +1.1
117,215 - EX31_RS00545 0.84 +0.1
117,252 + EX31_RS00545 0.89 +0.6
117,457 - EX31_RS00550 0.17 +2.1
117,570 + EX31_RS00550 0.35 +0.0
117,571 - EX31_RS00550 0.35 +0.7
117,594 + EX31_RS00550 0.39 +0.4
117,595 - EX31_RS00550 0.39 -2.7
117,595 - EX31_RS00550 0.39 -0.3
117,603 - EX31_RS00550 0.40 +1.2
117,752 - EX31_RS00550 0.64 -0.4
117,772 + EX31_RS00550 0.67 +0.1
117,772 + EX31_RS00550 0.67 +0.7
117,843 + EX31_RS00550 0.79 +0.5
117,843 + EX31_RS00550 0.79 +1.6
117,844 - EX31_RS00550 0.79 +0.8
117,868 + EX31_RS00550 0.83 -1.4
117,868 + EX31_RS00550 0.83 +0.9
117,869 - EX31_RS00550 0.83 +0.5
117,869 - EX31_RS00550 0.83 -0.4
117,968 + +2.1
117,968 + -1.2
117,968 + +0.5
117,968 + +1.5
117,969 - -0.5
117,976 + +1.3
118,013 + +1.6
118,013 + -0.4
118,056 - -1.0
118,088 + -1.1
118,089 - +1.5
118,135 + +0.7
118,135 + -1.2
118,222 + EX31_RS00555 0.13 -1.8
118,357 + EX31_RS00555 0.31 +0.3
118,357 + EX31_RS00555 0.31 +0.8
118,358 - EX31_RS00555 0.32 -0.3
118,369 + EX31_RS00555 0.33 +0.1
118,369 + EX31_RS00555 0.33 +0.5
118,369 + EX31_RS00555 0.33 -0.4
118,370 - EX31_RS00555 0.33 +1.7
118,370 - EX31_RS00555 0.33 -0.1
118,370 - EX31_RS00555 0.33 -1.4
118,433 + EX31_RS00555 0.42 +1.3
118,453 + EX31_RS00555 0.45 -0.7
118,453 + EX31_RS00555 0.45 -0.3
118,514 - EX31_RS00555 0.53 +0.6
118,635 - EX31_RS00555 0.70 -0.8
118,684 - EX31_RS00555 0.77 +0.9
118,715 + EX31_RS00555 0.81 +0.4
118,716 - EX31_RS00555 0.81 -0.5
118,723 + EX31_RS00555 0.82 -0.5
118,739 - EX31_RS00555 0.84 +1.1
118,761 + EX31_RS00555 0.87 +0.7
118,762 - EX31_RS00555 0.87 -0.1
118,850 + -0.8
118,850 + +0.5
118,851 - -0.5

Or see this region's nucleotide sequence