Strain Fitness in Rahnella sp. WP5 around EX31_RS00255

Experiment: Ammonium chloride (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS00250 and EX31_RS00255 are separated by 71 nucleotidesEX31_RS00255 and EX31_RS00260 are separated by 141 nucleotides EX31_RS00250: EX31_RS00250 - aldehyde dehydrogenase, at 51,730 to 53,169 _RS00250 EX31_RS00255: EX31_RS00255 - hypothetical protein, at 53,241 to 53,822 _RS00255 EX31_RS00260: EX31_RS00260 - hypothetical protein, at 53,964 to 55,040 _RS00260 Position (kb) 53 54Strain fitness (log2 ratio) -2 -1 0 1 2at 52.300 kb on - strand, within EX31_RS00250at 52.335 kb on + strand, within EX31_RS00250at 52.386 kb on + strand, within EX31_RS00250at 52.581 kb on + strand, within EX31_RS00250at 52.581 kb on + strand, within EX31_RS00250at 52.582 kb on - strand, within EX31_RS00250at 52.600 kb on + strand, within EX31_RS00250at 52.600 kb on + strand, within EX31_RS00250at 52.600 kb on + strand, within EX31_RS00250at 52.600 kb on + strand, within EX31_RS00250at 52.601 kb on - strand, within EX31_RS00250at 52.616 kb on + strand, within EX31_RS00250at 52.744 kb on - strand, within EX31_RS00250at 52.972 kb on + strand, within EX31_RS00250at 52.972 kb on + strand, within EX31_RS00250at 52.973 kb on - strand, within EX31_RS00250at 52.983 kb on + strand, within EX31_RS00250at 52.984 kb on - strand, within EX31_RS00250at 52.984 kb on - strand, within EX31_RS00250at 52.985 kb on + strand, within EX31_RS00250at 52.986 kb on - strand, within EX31_RS00250at 52.986 kb on - strand, within EX31_RS00250at 52.986 kb on - strand, within EX31_RS00250at 53.032 kb on + strandat 53.032 kb on + strandat 53.085 kb on + strandat 53.131 kb on + strandat 53.168 kb on - strandat 53.171 kb on + strandat 53.172 kb on - strandat 53.280 kb on - strandat 53.424 kb on + strand, within EX31_RS00255at 53.425 kb on - strand, within EX31_RS00255at 53.437 kb on + strand, within EX31_RS00255at 53.437 kb on + strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.439 kb on + strand, within EX31_RS00255at 53.440 kb on - strand, within EX31_RS00255at 53.440 kb on - strand, within EX31_RS00255at 53.481 kb on - strand, within EX31_RS00255at 53.722 kb on - strand, within EX31_RS00255at 53.729 kb on + strand, within EX31_RS00255at 53.730 kb on - strand, within EX31_RS00255at 53.730 kb on - strand, within EX31_RS00255at 53.730 kb on - strand, within EX31_RS00255at 53.820 kb on + strandat 53.821 kb on - strandat 53.862 kb on - strandat 53.875 kb on + strandat 53.875 kb on + strandat 53.884 kb on + strandat 53.891 kb on + strandat 53.974 kb on + strandat 54.068 kb on + strandat 54.069 kb on - strandat 54.137 kb on + strand, within EX31_RS00260at 54.137 kb on + strand, within EX31_RS00260at 54.159 kb on + strand, within EX31_RS00260at 54.161 kb on + strand, within EX31_RS00260at 54.161 kb on + strand, within EX31_RS00260at 54.162 kb on - strand, within EX31_RS00260at 54.213 kb on + strand, within EX31_RS00260at 54.213 kb on + strand, within EX31_RS00260at 54.213 kb on + strand, within EX31_RS00260at 54.267 kb on - strand, within EX31_RS00260at 54.308 kb on + strand, within EX31_RS00260at 54.308 kb on + strand, within EX31_RS00260at 54.309 kb on - strand, within EX31_RS00260at 54.346 kb on + strand, within EX31_RS00260at 54.346 kb on + strand, within EX31_RS00260at 54.453 kb on + strand, within EX31_RS00260at 54.560 kb on + strand, within EX31_RS00260at 54.597 kb on + strand, within EX31_RS00260at 54.597 kb on + strand, within EX31_RS00260at 54.597 kb on + strand, within EX31_RS00260at 54.598 kb on - strand, within EX31_RS00260at 54.598 kb on - strand, within EX31_RS00260at 54.658 kb on + strand, within EX31_RS00260at 54.684 kb on + strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.809 kb on + strand, within EX31_RS00260at 54.810 kb on - strand, within EX31_RS00260

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking
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52,300 - EX31_RS00250 0.40 +2.3
52,335 + EX31_RS00250 0.42 +0.4
52,386 + EX31_RS00250 0.46 +0.3
52,581 + EX31_RS00250 0.59 +1.1
52,581 + EX31_RS00250 0.59 +1.3
52,582 - EX31_RS00250 0.59 +0.8
52,600 + EX31_RS00250 0.60 +0.4
52,600 + EX31_RS00250 0.60 -0.3
52,600 + EX31_RS00250 0.60 +0.3
52,600 + EX31_RS00250 0.60 +1.4
52,601 - EX31_RS00250 0.60 +0.0
52,616 + EX31_RS00250 0.62 +0.1
52,744 - EX31_RS00250 0.70 +0.5
52,972 + EX31_RS00250 0.86 -0.7
52,972 + EX31_RS00250 0.86 -0.4
52,973 - EX31_RS00250 0.86 -0.4
52,983 + EX31_RS00250 0.87 -0.7
52,984 - EX31_RS00250 0.87 +0.8
52,984 - EX31_RS00250 0.87 +1.5
52,985 + EX31_RS00250 0.87 -0.4
52,986 - EX31_RS00250 0.87 -0.6
52,986 - EX31_RS00250 0.87 -0.3
52,986 - EX31_RS00250 0.87 +0.4
53,032 + +1.9
53,032 + -0.7
53,085 + +1.5
53,131 + -1.5
53,168 - +1.9
53,171 + -1.6
53,172 - +1.1
53,280 - -0.2
53,424 + EX31_RS00255 0.31 -0.4
53,425 - EX31_RS00255 0.32 -0.5
53,437 + EX31_RS00255 0.34 +1.3
53,437 + EX31_RS00255 0.34 +1.1
53,438 - EX31_RS00255 0.34 +0.4
53,438 - EX31_RS00255 0.34 -0.2
53,438 - EX31_RS00255 0.34 +0.3
53,438 - EX31_RS00255 0.34 -0.4
53,439 + EX31_RS00255 0.34 -0.5
53,440 - EX31_RS00255 0.34 -0.6
53,440 - EX31_RS00255 0.34 -2.6
53,481 - EX31_RS00255 0.41 +0.2
53,722 - EX31_RS00255 0.83 -1.0
53,729 + EX31_RS00255 0.84 +0.8
53,730 - EX31_RS00255 0.84 -0.1
53,730 - EX31_RS00255 0.84 -0.8
53,730 - EX31_RS00255 0.84 -1.1
53,820 + -0.5
53,821 - -0.6
53,862 - -0.6
53,875 + -0.7
53,875 + +0.2
53,884 + +1.5
53,891 + -1.4
53,974 + +1.0
54,068 + -0.0
54,069 - +0.8
54,137 + EX31_RS00260 0.16 -0.6
54,137 + EX31_RS00260 0.16 -0.7
54,159 + EX31_RS00260 0.18 +0.5
54,161 + EX31_RS00260 0.18 +0.4
54,161 + EX31_RS00260 0.18 -0.2
54,162 - EX31_RS00260 0.18 -1.4
54,213 + EX31_RS00260 0.23 +0.5
54,213 + EX31_RS00260 0.23 +0.3
54,213 + EX31_RS00260 0.23 -0.1
54,267 - EX31_RS00260 0.28 +0.3
54,308 + EX31_RS00260 0.32 +1.3
54,308 + EX31_RS00260 0.32 -1.1
54,309 - EX31_RS00260 0.32 -0.7
54,346 + EX31_RS00260 0.35 -1.9
54,346 + EX31_RS00260 0.35 +0.1
54,453 + EX31_RS00260 0.45 -1.9
54,560 + EX31_RS00260 0.55 -0.2
54,597 + EX31_RS00260 0.59 +0.3
54,597 + EX31_RS00260 0.59 +1.2
54,597 + EX31_RS00260 0.59 +0.6
54,598 - EX31_RS00260 0.59 +0.9
54,598 - EX31_RS00260 0.59 +1.0
54,658 + EX31_RS00260 0.64 -0.1
54,684 + EX31_RS00260 0.67 +0.1
54,685 - EX31_RS00260 0.67 -1.1
54,685 - EX31_RS00260 0.67 +0.1
54,685 - EX31_RS00260 0.67 +0.8
54,685 - EX31_RS00260 0.67 +0.3
54,809 + EX31_RS00260 0.78 +1.4
54,810 - EX31_RS00260 0.79 +0.8

Or see this region's nucleotide sequence