Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24725 and EX31_RS24730 are separated by 161 nucleotides EX31_RS24730 and EX31_RS24735 are separated by 90 nucleotides EX31_RS24735 and EX31_RS24740 are separated by 250 nucleotides
EX31_RS24725: EX31_RS24725 - aminodeoxychorismate synthase component 1, at 714,880 to 716,256
_RS24725
EX31_RS24730: EX31_RS24730 - YoaH family protein, at 716,418 to 716,600
_RS24730
EX31_RS24735: EX31_RS24735 - formate-dependent phosphoribosylglycinamide formyltransferase, at 716,691 to 717,869
_RS24735
EX31_RS24740: EX31_RS24740 - ATP-dependent RNA helicase DbpA, at 718,120 to 719,502
_RS24740
Position (kb)
716
717
718 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 715.711 kb on + strand, within EX31_RS24725 at 715.712 kb on - strand, within EX31_RS24725 at 715.712 kb on - strand, within EX31_RS24725 at 715.762 kb on + strand, within EX31_RS24725 at 715.763 kb on - strand, within EX31_RS24725 at 715.807 kb on + strand, within EX31_RS24725 at 715.808 kb on - strand, within EX31_RS24725 at 715.808 kb on - strand, within EX31_RS24725 at 715.838 kb on + strand, within EX31_RS24725 at 715.839 kb on - strand, within EX31_RS24725 at 715.839 kb on - strand, within EX31_RS24725 at 715.839 kb on - strand, within EX31_RS24725 at 715.978 kb on + strand, within EX31_RS24725 at 716.066 kb on - strand, within EX31_RS24725 at 716.125 kb on - strand at 716.125 kb on - strand at 716.155 kb on + strand at 716.155 kb on + strand at 716.156 kb on - strand at 716.201 kb on - strand at 716.201 kb on - strand at 716.221 kb on + strand at 716.221 kb on + strand at 716.222 kb on - strand at 716.288 kb on - strand at 716.356 kb on + strand at 716.356 kb on + strand at 716.356 kb on + strand at 716.357 kb on - strand at 716.357 kb on - strand at 716.358 kb on + strand at 716.358 kb on + strand at 716.359 kb on - strand at 716.402 kb on - strand at 716.429 kb on + strand at 716.429 kb on + strand at 716.429 kb on + strand at 716.430 kb on - strand at 716.430 kb on - strand at 716.430 kb on - strand at 716.430 kb on - strand at 716.440 kb on + strand, within EX31_RS24730 at 716.441 kb on - strand, within EX31_RS24730 at 716.441 kb on - strand, within EX31_RS24730 at 716.552 kb on + strand, within EX31_RS24730 at 716.552 kb on + strand, within EX31_RS24730 at 716.552 kb on + strand, within EX31_RS24730 at 716.599 kb on - strand at 716.790 kb on - strand at 716.980 kb on - strand, within EX31_RS24735 at 717.257 kb on + strand, within EX31_RS24735 at 717.332 kb on - strand, within EX31_RS24735 at 717.332 kb on - strand, within EX31_RS24735 at 717.476 kb on - strand, within EX31_RS24735 at 717.689 kb on + strand, within EX31_RS24735 at 717.692 kb on + strand, within EX31_RS24735 at 717.692 kb on + strand, within EX31_RS24735 at 717.693 kb on - strand, within EX31_RS24735 at 717.693 kb on - strand, within EX31_RS24735 at 717.740 kb on - strand, within EX31_RS24735 at 717.816 kb on + strand at 717.816 kb on + strand at 717.817 kb on - strand at 717.817 kb on - strand at 717.820 kb on - strand at 717.820 kb on - strand at 717.820 kb on - strand at 717.854 kb on + strand at 717.864 kb on + strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.922 kb on + strand at 717.923 kb on - strand at 717.933 kb on - strand at 717.973 kb on + strand at 718.105 kb on - strand at 718.105 kb on - strand at 718.105 kb on - strand at 718.112 kb on + strand at 718.112 kb on + strand at 718.189 kb on + strand at 718.278 kb on + strand, within EX31_RS24740 at 718.349 kb on + strand, within EX31_RS24740 at 718.349 kb on + strand, within EX31_RS24740 at 718.394 kb on + strand, within EX31_RS24740 at 718.395 kb on - strand, within EX31_RS24740 at 718.519 kb on - strand, within EX31_RS24740 at 718.568 kb on + strand, within EX31_RS24740 at 718.568 kb on + strand, within EX31_RS24740 at 718.568 kb on + strand, within EX31_RS24740 at 718.569 kb on - strand, within EX31_RS24740 at 718.569 kb on - strand, within EX31_RS24740 at 718.569 kb on - strand, within EX31_RS24740 at 718.620 kb on + strand, within EX31_RS24740 at 718.639 kb on + strand, within EX31_RS24740 at 718.641 kb on + strand, within EX31_RS24740 at 718.641 kb on + strand, within EX31_RS24740 at 718.642 kb on - strand, within EX31_RS24740 at 718.736 kb on + strand, within EX31_RS24740 at 718.767 kb on + strand, within EX31_RS24740 at 718.767 kb on + strand, within EX31_RS24740 at 718.831 kb on + strand, within EX31_RS24740 at 718.831 kb on + strand, within EX31_RS24740 at 718.832 kb on - strand, within EX31_RS24740 at 718.837 kb on + strand, within EX31_RS24740 at 718.866 kb on + strand, within EX31_RS24740
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 715,711 + EX31_RS24725 0.60 +1.4 715,712 - EX31_RS24725 0.60 -0.7 715,712 - EX31_RS24725 0.60 -0.7 715,762 + EX31_RS24725 0.64 +1.7 715,763 - EX31_RS24725 0.64 +1.1 715,807 + EX31_RS24725 0.67 -0.4 715,808 - EX31_RS24725 0.67 -0.4 715,808 - EX31_RS24725 0.67 -1.8 715,838 + EX31_RS24725 0.70 -1.2 715,839 - EX31_RS24725 0.70 +0.9 715,839 - EX31_RS24725 0.70 +0.1 715,839 - EX31_RS24725 0.70 +0.3 715,978 + EX31_RS24725 0.80 +1.9 716,066 - EX31_RS24725 0.86 +0.9 716,125 - -0.0 716,125 - +0.5 716,155 + +0.9 716,155 + -0.5 716,156 - -1.7 716,201 - +0.2 716,201 - -0.5 716,221 + +0.1 716,221 + -0.1 716,222 - +1.2 716,288 - +2.5 716,356 + +0.6 716,356 + -0.4 716,356 + +0.8 716,357 - +0.4 716,357 - -0.4 716,358 + -1.2 716,358 + -1.8 716,359 - -1.5 716,402 - +0.1 716,429 + -0.9 716,429 + +0.5 716,429 + +0.6 716,430 - -0.6 716,430 - +0.9 716,430 - +0.0 716,430 - -0.4 716,440 + EX31_RS24730 0.12 +0.3 716,441 - EX31_RS24730 0.13 -0.2 716,441 - EX31_RS24730 0.13 +0.9 716,552 + EX31_RS24730 0.73 +0.7 716,552 + EX31_RS24730 0.73 -0.1 716,552 + EX31_RS24730 0.73 +0.4 716,599 - -0.4 716,790 - +0.8 716,980 - EX31_RS24735 0.25 -1.3 717,257 + EX31_RS24735 0.48 +1.2 717,332 - EX31_RS24735 0.54 -1.4 717,332 - EX31_RS24735 0.54 +0.3 717,476 - EX31_RS24735 0.67 +0.5 717,689 + EX31_RS24735 0.85 +0.6 717,692 + EX31_RS24735 0.85 -0.4 717,692 + EX31_RS24735 0.85 -0.1 717,693 - EX31_RS24735 0.85 -0.5 717,693 - EX31_RS24735 0.85 -0.5 717,740 - EX31_RS24735 0.89 -0.3 717,816 + -0.5 717,816 + +0.5 717,817 - +0.1 717,817 - -1.0 717,820 - -0.6 717,820 - -0.2 717,820 - +0.2 717,854 + +0.9 717,864 + +0.1 717,869 + +1.3 717,869 + +0.8 717,870 - -0.4 717,870 - +0.5 717,920 + +0.3 717,920 + +1.7 717,920 + -1.7 717,920 + -0.4 717,920 + -0.2 717,920 + +0.1 717,920 + +0.0 717,920 + +0.3 717,920 + -0.1 717,921 - +0.9 717,921 - +1.4 717,921 - +1.6 717,921 - +1.9 717,921 - -1.5 717,921 - -0.2 717,921 - -0.3 717,921 - +0.4 717,921 - -0.7 717,922 + +1.0 717,923 - -0.8 717,933 - +2.3 717,973 + -0.9 718,105 - +1.3 718,105 - +0.4 718,105 - -1.0 718,112 + +0.9 718,112 + -0.4 718,189 + -0.2 718,278 + EX31_RS24740 0.11 +0.1 718,349 + EX31_RS24740 0.17 +0.5 718,349 + EX31_RS24740 0.17 +2.3 718,394 + EX31_RS24740 0.20 +0.9 718,395 - EX31_RS24740 0.20 -1.2 718,519 - EX31_RS24740 0.29 +0.2 718,568 + EX31_RS24740 0.32 +1.9 718,568 + EX31_RS24740 0.32 +0.8 718,568 + EX31_RS24740 0.32 -1.1 718,569 - EX31_RS24740 0.32 -0.5 718,569 - EX31_RS24740 0.32 -1.8 718,569 - EX31_RS24740 0.32 +0.2 718,620 + EX31_RS24740 0.36 -0.4 718,639 + EX31_RS24740 0.38 -0.1 718,641 + EX31_RS24740 0.38 -1.1 718,641 + EX31_RS24740 0.38 +0.5 718,642 - EX31_RS24740 0.38 +1.1 718,736 + EX31_RS24740 0.45 -0.2 718,767 + EX31_RS24740 0.47 +0.5 718,767 + EX31_RS24740 0.47 +1.0 718,831 + EX31_RS24740 0.51 +1.2 718,831 + EX31_RS24740 0.51 -1.1 718,832 - EX31_RS24740 0.51 -0.8 718,837 + EX31_RS24740 0.52 +0.1 718,866 + EX31_RS24740 0.54 -2.3
Or see this region's nucleotide sequence