Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22825 and EX31_RS22830 are separated by 99 nucleotides EX31_RS22830 and EX31_RS22835 are separated by 32 nucleotides EX31_RS22835 and EX31_RS22840 are separated by 185 nucleotides
EX31_RS22825: EX31_RS22825 - DUF1176 domain-containing protein, at 326,126 to 327,193
_RS22825
EX31_RS22830: EX31_RS22830 - ribosome-associated protein YbcJ, at 327,293 to 327,511
_RS22830
EX31_RS22835: EX31_RS22835 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 327,544 to 328,416
_RS22835
EX31_RS22840: EX31_RS22840 - L-lactate dehydrogenase, at 328,602 to 329,513
_RS22840
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1
2 at 326.647 kb on + strand, within EX31_RS22825 at 326.648 kb on - strand, within EX31_RS22825 at 326.672 kb on - strand, within EX31_RS22825 at 326.683 kb on + strand, within EX31_RS22825 at 326.785 kb on + strand, within EX31_RS22825 at 326.824 kb on + strand, within EX31_RS22825 at 326.825 kb on - strand, within EX31_RS22825 at 326.825 kb on - strand, within EX31_RS22825 at 326.825 kb on - strand, within EX31_RS22825 at 326.882 kb on + strand, within EX31_RS22825 at 326.882 kb on + strand, within EX31_RS22825 at 326.882 kb on + strand, within EX31_RS22825 at 326.883 kb on - strand, within EX31_RS22825 at 326.883 kb on - strand, within EX31_RS22825 at 326.883 kb on - strand, within EX31_RS22825 at 326.885 kb on - strand, within EX31_RS22825 at 326.885 kb on - strand, within EX31_RS22825 at 326.891 kb on + strand, within EX31_RS22825 at 326.892 kb on - strand, within EX31_RS22825 at 326.927 kb on + strand, within EX31_RS22825 at 326.929 kb on + strand, within EX31_RS22825 at 326.929 kb on + strand, within EX31_RS22825 at 326.929 kb on + strand, within EX31_RS22825 at 326.953 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.043 kb on + strand, within EX31_RS22825 at 327.097 kb on + strand at 327.097 kb on + strand at 327.097 kb on + strand at 327.098 kb on - strand at 327.146 kb on + strand at 327.175 kb on - strand at 327.344 kb on - strand, within EX31_RS22830 at 327.344 kb on - strand, within EX31_RS22830 at 327.514 kb on - strand at 327.514 kb on - strand at 327.514 kb on - strand at 327.514 kb on - strand at 327.514 kb on - strand at 327.546 kb on - strand at 327.553 kb on - strand at 327.553 kb on - strand at 327.572 kb on + strand at 327.572 kb on + strand at 327.572 kb on + strand at 327.572 kb on + strand at 328.434 kb on - strand at 328.438 kb on - strand at 328.438 kb on - strand at 328.438 kb on - strand at 328.505 kb on - strand at 328.512 kb on + strand at 328.559 kb on + strand at 328.559 kb on + strand at 328.560 kb on - strand at 328.560 kb on - strand at 328.564 kb on + strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.581 kb on - strand at 328.715 kb on + strand, within EX31_RS22840 at 328.715 kb on + strand, within EX31_RS22840 at 328.715 kb on + strand, within EX31_RS22840 at 328.715 kb on + strand, within EX31_RS22840 at 328.716 kb on - strand, within EX31_RS22840 at 328.716 kb on - strand, within EX31_RS22840 at 328.716 kb on - strand, within EX31_RS22840 at 328.795 kb on - strand, within EX31_RS22840 at 329.291 kb on + strand, within EX31_RS22840 at 329.379 kb on + strand, within EX31_RS22840 at 329.379 kb on + strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.414 kb on + strand, within EX31_RS22840 at 329.415 kb on - strand, within EX31_RS22840
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 326,647 + EX31_RS22825 0.49 +0.1 326,648 - EX31_RS22825 0.49 -0.4 326,672 - EX31_RS22825 0.51 -0.3 326,683 + EX31_RS22825 0.52 +1.3 326,785 + EX31_RS22825 0.62 +1.2 326,824 + EX31_RS22825 0.65 -1.2 326,825 - EX31_RS22825 0.65 +0.7 326,825 - EX31_RS22825 0.65 -0.5 326,825 - EX31_RS22825 0.65 +1.7 326,882 + EX31_RS22825 0.71 +0.0 326,882 + EX31_RS22825 0.71 -0.0 326,882 + EX31_RS22825 0.71 +2.1 326,883 - EX31_RS22825 0.71 -0.4 326,883 - EX31_RS22825 0.71 -1.3 326,883 - EX31_RS22825 0.71 +0.2 326,885 - EX31_RS22825 0.71 +0.8 326,885 - EX31_RS22825 0.71 +1.4 326,891 + EX31_RS22825 0.72 -0.6 326,892 - EX31_RS22825 0.72 +0.6 326,927 + EX31_RS22825 0.75 +0.1 326,929 + EX31_RS22825 0.75 -0.5 326,929 + EX31_RS22825 0.75 +1.4 326,929 + EX31_RS22825 0.75 +0.8 326,953 + EX31_RS22825 0.77 +0.8 327,038 + EX31_RS22825 0.85 -0.8 327,038 + EX31_RS22825 0.85 -0.6 327,038 + EX31_RS22825 0.85 -1.2 327,038 + EX31_RS22825 0.85 +0.2 327,043 + EX31_RS22825 0.86 +0.2 327,097 + +1.0 327,097 + -0.1 327,097 + +0.2 327,098 - -1.3 327,146 + +0.4 327,175 - -1.5 327,344 - EX31_RS22830 0.23 +0.4 327,344 - EX31_RS22830 0.23 -1.0 327,514 - -0.3 327,514 - +0.9 327,514 - +0.6 327,514 - +0.0 327,514 - -0.4 327,546 - +1.2 327,553 - +0.6 327,553 - -0.4 327,572 + -0.2 327,572 + -0.2 327,572 + -0.4 327,572 + -1.5 328,434 - +0.0 328,438 - +0.3 328,438 - +0.7 328,438 - +0.7 328,505 - -1.2 328,512 + +0.8 328,559 + +0.4 328,559 + -0.5 328,560 - +0.7 328,560 - +0.9 328,564 + +1.2 328,568 + +0.4 328,568 + -0.7 328,581 - +0.9 328,715 + EX31_RS22840 0.12 -0.3 328,715 + EX31_RS22840 0.12 +0.4 328,715 + EX31_RS22840 0.12 +1.0 328,715 + EX31_RS22840 0.12 +0.5 328,716 - EX31_RS22840 0.12 +0.2 328,716 - EX31_RS22840 0.12 +1.8 328,716 - EX31_RS22840 0.12 -0.3 328,795 - EX31_RS22840 0.21 +0.5 329,291 + EX31_RS22840 0.76 -0.6 329,379 + EX31_RS22840 0.85 +0.3 329,379 + EX31_RS22840 0.85 +0.4 329,380 - EX31_RS22840 0.85 -2.7 329,380 - EX31_RS22840 0.85 -1.7 329,380 - EX31_RS22840 0.85 +1.0 329,380 - EX31_RS22840 0.85 -0.3 329,414 + EX31_RS22840 0.89 +1.4 329,415 - EX31_RS22840 0.89 -1.0
Or see this region's nucleotide sequence