Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22820 and EX31_RS22825 are separated by 255 nucleotides EX31_RS22825 and EX31_RS22830 are separated by 99 nucleotides EX31_RS22830 and EX31_RS22835 are separated by 32 nucleotides
EX31_RS22820: EX31_RS22820 - hypothetical protein, at 324,695 to 325,870
_RS22820
EX31_RS22825: EX31_RS22825 - DUF1176 domain-containing protein, at 326,126 to 327,193
_RS22825
EX31_RS22830: EX31_RS22830 - ribosome-associated protein YbcJ, at 327,293 to 327,511
_RS22830
EX31_RS22835: EX31_RS22835 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 327,544 to 328,416
_RS22835
Position (kb) 326
327
328 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 325.158 kb on + strand, within EX31_RS22820 at 325.158 kb on + strand, within EX31_RS22820 at 325.158 kb on + strand, within EX31_RS22820 at 325.159 kb on - strand, within EX31_RS22820 at 325.159 kb on - strand, within EX31_RS22820 at 325.159 kb on - strand, within EX31_RS22820 at 325.165 kb on + strand, within EX31_RS22820 at 325.166 kb on - strand, within EX31_RS22820 at 325.166 kb on - strand, within EX31_RS22820 at 325.166 kb on - strand, within EX31_RS22820 at 325.169 kb on + strand, within EX31_RS22820 at 325.169 kb on + strand, within EX31_RS22820 at 325.169 kb on + strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.225 kb on - strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.458 kb on + strand, within EX31_RS22820 at 325.458 kb on + strand, within EX31_RS22820 at 325.459 kb on - strand, within EX31_RS22820 at 325.630 kb on + strand, within EX31_RS22820 at 325.659 kb on - strand, within EX31_RS22820 at 325.711 kb on - strand, within EX31_RS22820 at 325.711 kb on - strand, within EX31_RS22820 at 325.711 kb on - strand, within EX31_RS22820 at 325.711 kb on - strand, within EX31_RS22820 at 325.719 kb on + strand, within EX31_RS22820 at 325.725 kb on + strand, within EX31_RS22820 at 325.726 kb on - strand, within EX31_RS22820 at 325.726 kb on - strand, within EX31_RS22820 at 325.755 kb on - strand at 325.800 kb on + strand at 325.801 kb on - strand at 325.801 kb on - strand at 325.839 kb on - strand at 325.839 kb on - strand at 325.848 kb on - strand at 325.863 kb on - strand at 325.911 kb on + strand at 325.976 kb on + strand at 325.976 kb on + strand at 325.976 kb on + strand at 325.976 kb on + strand at 325.976 kb on + strand at 325.976 kb on + strand at 325.976 kb on + strand at 325.977 kb on - strand at 325.977 kb on - strand at 325.977 kb on - strand at 325.977 kb on - strand at 325.977 kb on - strand at 325.977 kb on - strand at 326.022 kb on - strand at 326.080 kb on + strand at 326.086 kb on - strand at 326.088 kb on + strand at 326.088 kb on + strand at 326.099 kb on + strand at 326.100 kb on - strand at 326.122 kb on + strand at 326.122 kb on + strand at 326.122 kb on + strand at 326.122 kb on + strand at 326.122 kb on + strand at 326.123 kb on - strand at 326.123 kb on - strand at 326.123 kb on - strand at 326.123 kb on - strand at 326.123 kb on - strand at 326.123 kb on - strand at 326.123 kb on - strand at 326.124 kb on + strand at 326.124 kb on + strand at 326.124 kb on + strand at 326.124 kb on + strand at 326.125 kb on - strand at 326.125 kb on - strand at 326.125 kb on - strand at 326.125 kb on - strand at 326.151 kb on + strand at 326.151 kb on + strand at 326.206 kb on + strand at 326.206 kb on + strand at 326.207 kb on - strand at 326.210 kb on + strand at 326.210 kb on + strand at 326.210 kb on + strand at 326.211 kb on - strand at 326.211 kb on - strand at 326.211 kb on - strand at 326.343 kb on - strand, within EX31_RS22825 at 326.350 kb on + strand, within EX31_RS22825 at 326.351 kb on - strand, within EX31_RS22825 at 326.407 kb on + strand, within EX31_RS22825 at 326.408 kb on - strand, within EX31_RS22825 at 326.422 kb on + strand, within EX31_RS22825 at 326.423 kb on - strand, within EX31_RS22825 at 326.446 kb on + strand, within EX31_RS22825 at 326.446 kb on + strand, within EX31_RS22825 at 326.491 kb on + strand, within EX31_RS22825 at 326.647 kb on + strand, within EX31_RS22825 at 326.648 kb on - strand, within EX31_RS22825 at 326.672 kb on - strand, within EX31_RS22825 at 326.683 kb on + strand, within EX31_RS22825 at 326.785 kb on + strand, within EX31_RS22825 at 326.824 kb on + strand, within EX31_RS22825 at 326.825 kb on - strand, within EX31_RS22825 at 326.825 kb on - strand, within EX31_RS22825 at 326.825 kb on - strand, within EX31_RS22825 at 326.882 kb on + strand, within EX31_RS22825 at 326.882 kb on + strand, within EX31_RS22825 at 326.882 kb on + strand, within EX31_RS22825 at 326.883 kb on - strand, within EX31_RS22825 at 326.883 kb on - strand, within EX31_RS22825 at 326.883 kb on - strand, within EX31_RS22825 at 326.885 kb on - strand, within EX31_RS22825 at 326.885 kb on - strand, within EX31_RS22825 at 326.891 kb on + strand, within EX31_RS22825 at 326.892 kb on - strand, within EX31_RS22825 at 326.927 kb on + strand, within EX31_RS22825 at 326.929 kb on + strand, within EX31_RS22825 at 326.929 kb on + strand, within EX31_RS22825 at 326.929 kb on + strand, within EX31_RS22825 at 326.953 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.038 kb on + strand, within EX31_RS22825 at 327.043 kb on + strand, within EX31_RS22825 at 327.097 kb on + strand at 327.097 kb on + strand at 327.097 kb on + strand at 327.098 kb on - strand at 327.146 kb on + strand at 327.175 kb on - strand at 327.344 kb on - strand, within EX31_RS22830 at 327.344 kb on - strand, within EX31_RS22830 at 327.514 kb on - strand at 327.514 kb on - strand at 327.514 kb on - strand at 327.514 kb on - strand at 327.514 kb on - strand at 327.546 kb on - strand at 327.553 kb on - strand at 327.553 kb on - strand at 327.572 kb on + strand at 327.572 kb on + strand at 327.572 kb on + strand at 327.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 325,158 + EX31_RS22820 0.39 -0.1 325,158 + EX31_RS22820 0.39 -0.2 325,158 + EX31_RS22820 0.39 +0.2 325,159 - EX31_RS22820 0.39 -0.5 325,159 - EX31_RS22820 0.39 +0.0 325,159 - EX31_RS22820 0.39 +0.7 325,165 + EX31_RS22820 0.40 +1.3 325,166 - EX31_RS22820 0.40 +0.8 325,166 - EX31_RS22820 0.40 +0.1 325,166 - EX31_RS22820 0.40 -0.5 325,169 + EX31_RS22820 0.40 +0.0 325,169 + EX31_RS22820 0.40 -0.3 325,169 + EX31_RS22820 0.40 +2.0 325,170 - EX31_RS22820 0.40 +0.2 325,170 - EX31_RS22820 0.40 -0.3 325,170 - EX31_RS22820 0.40 +1.0 325,170 - EX31_RS22820 0.40 +1.9 325,170 - EX31_RS22820 0.40 +0.4 325,170 - EX31_RS22820 0.40 -0.1 325,225 - EX31_RS22820 0.45 -0.4 325,258 + EX31_RS22820 0.48 +0.5 325,258 + EX31_RS22820 0.48 -1.0 325,258 + EX31_RS22820 0.48 -0.3 325,258 + EX31_RS22820 0.48 +0.6 325,259 - EX31_RS22820 0.48 +0.7 325,259 - EX31_RS22820 0.48 +1.3 325,259 - EX31_RS22820 0.48 -0.2 325,259 - EX31_RS22820 0.48 -1.0 325,259 - EX31_RS22820 0.48 +0.7 325,259 - EX31_RS22820 0.48 -0.3 325,259 - EX31_RS22820 0.48 +1.2 325,327 + EX31_RS22820 0.54 -1.0 325,327 + EX31_RS22820 0.54 +0.4 325,327 + EX31_RS22820 0.54 +1.4 325,327 + EX31_RS22820 0.54 -0.9 325,328 - EX31_RS22820 0.54 +0.6 325,328 - EX31_RS22820 0.54 +1.6 325,328 - EX31_RS22820 0.54 -0.9 325,328 - EX31_RS22820 0.54 +0.8 325,328 - EX31_RS22820 0.54 +0.0 325,458 + EX31_RS22820 0.65 +1.2 325,458 + EX31_RS22820 0.65 -0.3 325,459 - EX31_RS22820 0.65 +2.0 325,630 + EX31_RS22820 0.80 +0.1 325,659 - EX31_RS22820 0.82 -1.0 325,711 - EX31_RS22820 0.86 +0.1 325,711 - EX31_RS22820 0.86 -0.6 325,711 - EX31_RS22820 0.86 -1.1 325,711 - EX31_RS22820 0.86 +0.8 325,719 + EX31_RS22820 0.87 -0.9 325,725 + EX31_RS22820 0.88 +0.3 325,726 - EX31_RS22820 0.88 -1.1 325,726 - EX31_RS22820 0.88 -1.0 325,755 - +0.3 325,800 + -0.5 325,801 - -0.6 325,801 - -0.8 325,839 - +2.0 325,839 - +0.3 325,848 - +0.0 325,863 - -0.1 325,911 + -1.1 325,976 + +1.5 325,976 + -0.2 325,976 + +0.3 325,976 + +0.8 325,976 + +0.1 325,976 + -0.2 325,976 + -0.0 325,977 - -0.2 325,977 - -0.3 325,977 - +0.0 325,977 - -1.0 325,977 - +0.2 325,977 - +0.7 326,022 - -0.5 326,080 + -2.0 326,086 - -2.2 326,088 + +2.2 326,088 + +0.1 326,099 + +0.2 326,100 - -0.2 326,122 + +0.4 326,122 + -0.0 326,122 + -1.1 326,122 + +0.3 326,122 + +0.4 326,123 - +0.4 326,123 - +0.0 326,123 - +0.2 326,123 - -1.2 326,123 - +0.4 326,123 - +0.6 326,123 - +1.7 326,124 + +0.9 326,124 + -0.3 326,124 + -1.4 326,124 + +3.0 326,125 - +1.0 326,125 - -0.1 326,125 - +0.4 326,125 - +0.7 326,151 + -0.1 326,151 + +0.3 326,206 + +0.7 326,206 + -0.4 326,207 - +0.8 326,210 + -0.2 326,210 + -0.2 326,210 + +0.3 326,211 - -0.7 326,211 - +0.7 326,211 - +0.5 326,343 - EX31_RS22825 0.20 +0.1 326,350 + EX31_RS22825 0.21 +0.3 326,351 - EX31_RS22825 0.21 -0.3 326,407 + EX31_RS22825 0.26 +0.7 326,408 - EX31_RS22825 0.26 +2.0 326,422 + EX31_RS22825 0.28 +0.2 326,423 - EX31_RS22825 0.28 +0.0 326,446 + EX31_RS22825 0.30 -0.1 326,446 + EX31_RS22825 0.30 +0.3 326,491 + EX31_RS22825 0.34 +0.0 326,647 + EX31_RS22825 0.49 +0.1 326,648 - EX31_RS22825 0.49 -0.4 326,672 - EX31_RS22825 0.51 -0.3 326,683 + EX31_RS22825 0.52 +1.3 326,785 + EX31_RS22825 0.62 +1.2 326,824 + EX31_RS22825 0.65 -1.2 326,825 - EX31_RS22825 0.65 +0.7 326,825 - EX31_RS22825 0.65 -0.5 326,825 - EX31_RS22825 0.65 +1.7 326,882 + EX31_RS22825 0.71 +0.0 326,882 + EX31_RS22825 0.71 -0.0 326,882 + EX31_RS22825 0.71 +2.1 326,883 - EX31_RS22825 0.71 -0.4 326,883 - EX31_RS22825 0.71 -1.3 326,883 - EX31_RS22825 0.71 +0.2 326,885 - EX31_RS22825 0.71 +0.8 326,885 - EX31_RS22825 0.71 +1.4 326,891 + EX31_RS22825 0.72 -0.6 326,892 - EX31_RS22825 0.72 +0.6 326,927 + EX31_RS22825 0.75 +0.1 326,929 + EX31_RS22825 0.75 -0.5 326,929 + EX31_RS22825 0.75 +1.4 326,929 + EX31_RS22825 0.75 +0.8 326,953 + EX31_RS22825 0.77 +0.8 327,038 + EX31_RS22825 0.85 -0.8 327,038 + EX31_RS22825 0.85 -0.6 327,038 + EX31_RS22825 0.85 -1.2 327,038 + EX31_RS22825 0.85 +0.2 327,043 + EX31_RS22825 0.86 +0.2 327,097 + +1.0 327,097 + -0.1 327,097 + +0.2 327,098 - -1.3 327,146 + +0.4 327,175 - -1.5 327,344 - EX31_RS22830 0.23 +0.4 327,344 - EX31_RS22830 0.23 -1.0 327,514 - -0.3 327,514 - +0.9 327,514 - +0.6 327,514 - +0.0 327,514 - -0.4 327,546 - +1.2 327,553 - +0.6 327,553 - -0.4 327,572 + -0.2 327,572 + -0.2 327,572 + -0.4 327,572 + -1.5
Or see this region's nucleotide sequence