Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22680 and EX31_RS22685 are separated by 309 nucleotides EX31_RS22685 and EX31_RS22690 are separated by 220 nucleotides EX31_RS22690 and EX31_RS22695 are separated by 36 nucleotides
EX31_RS22680: EX31_RS22680 - polyphosphate kinase 1, at 295,033 to 297,102
_RS22680
EX31_RS22685: EX31_RS22685 - spermidine N1-acetyltransferase, at 297,412 to 297,987
_RS22685
EX31_RS22690: EX31_RS22690 - phosphoribosylglycinamide formyltransferase, at 298,208 to 298,846
_RS22690
EX31_RS22695: EX31_RS22695 - phosphoribosylformylglycinamidine cyclo-ligase, at 298,883 to 299,920
_RS22695
Position (kb) 297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 296.435 kb on + strand, within EX31_RS22680 at 296.436 kb on - strand, within EX31_RS22680 at 296.637 kb on - strand, within EX31_RS22680 at 296.642 kb on + strand, within EX31_RS22680 at 296.642 kb on + strand, within EX31_RS22680 at 296.643 kb on - strand, within EX31_RS22680 at 296.726 kb on + strand, within EX31_RS22680 at 296.932 kb on + strand at 296.932 kb on + strand at 296.952 kb on + strand at 296.955 kb on - strand at 296.955 kb on - strand at 296.978 kb on + strand at 296.978 kb on + strand at 296.979 kb on - strand at 296.979 kb on - strand at 296.979 kb on - strand at 297.068 kb on - strand at 297.068 kb on - strand at 297.068 kb on - strand at 297.068 kb on - strand at 297.082 kb on - strand at 297.082 kb on - strand at 297.083 kb on + strand at 297.120 kb on - strand at 297.138 kb on + strand at 297.138 kb on + strand at 297.139 kb on - strand at 297.139 kb on - strand at 297.139 kb on - strand at 297.170 kb on + strand at 297.170 kb on + strand at 297.170 kb on + strand at 297.170 kb on + strand at 297.171 kb on - strand at 297.171 kb on - strand at 297.171 kb on - strand at 297.171 kb on - strand at 297.171 kb on - strand at 297.171 kb on - strand at 297.180 kb on + strand at 297.180 kb on + strand at 297.180 kb on + strand at 297.181 kb on - strand at 297.181 kb on - strand at 297.181 kb on - strand at 297.181 kb on - strand at 297.181 kb on - strand at 297.181 kb on - strand at 297.204 kb on - strand at 297.244 kb on + strand at 297.245 kb on - strand at 297.251 kb on + strand at 297.251 kb on + strand at 297.251 kb on + strand at 297.252 kb on - strand at 297.252 kb on - strand at 297.252 kb on - strand at 297.252 kb on - strand at 297.254 kb on + strand at 297.255 kb on - strand at 297.255 kb on - strand at 297.291 kb on + strand at 297.291 kb on + strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.385 kb on - strand at 297.414 kb on - strand at 297.498 kb on + strand, within EX31_RS22685 at 297.498 kb on + strand, within EX31_RS22685 at 297.499 kb on - strand, within EX31_RS22685 at 297.499 kb on - strand, within EX31_RS22685 at 297.522 kb on - strand, within EX31_RS22685 at 297.584 kb on + strand, within EX31_RS22685 at 297.585 kb on - strand, within EX31_RS22685 at 297.585 kb on - strand, within EX31_RS22685 at 297.622 kb on + strand, within EX31_RS22685 at 297.622 kb on + strand, within EX31_RS22685 at 297.623 kb on - strand, within EX31_RS22685 at 297.623 kb on - strand, within EX31_RS22685 at 297.627 kb on - strand, within EX31_RS22685 at 297.627 kb on - strand, within EX31_RS22685 at 297.627 kb on - strand, within EX31_RS22685 at 297.635 kb on + strand, within EX31_RS22685 at 297.636 kb on - strand, within EX31_RS22685 at 297.659 kb on + strand, within EX31_RS22685 at 297.660 kb on - strand, within EX31_RS22685 at 297.660 kb on - strand, within EX31_RS22685 at 297.750 kb on + strand, within EX31_RS22685 at 297.751 kb on - strand, within EX31_RS22685 at 297.751 kb on - strand, within EX31_RS22685 at 297.751 kb on - strand, within EX31_RS22685 at 297.751 kb on - strand, within EX31_RS22685 at 297.756 kb on + strand, within EX31_RS22685 at 297.757 kb on - strand, within EX31_RS22685 at 297.807 kb on + strand, within EX31_RS22685 at 297.807 kb on + strand, within EX31_RS22685 at 297.834 kb on + strand, within EX31_RS22685 at 297.835 kb on - strand, within EX31_RS22685 at 297.835 kb on - strand, within EX31_RS22685 at 297.835 kb on - strand, within EX31_RS22685 at 297.835 kb on - strand, within EX31_RS22685 at 297.835 kb on - strand, within EX31_RS22685 at 297.845 kb on + strand, within EX31_RS22685 at 297.845 kb on + strand, within EX31_RS22685 at 297.845 kb on + strand, within EX31_RS22685 at 297.846 kb on - strand, within EX31_RS22685 at 297.875 kb on + strand, within EX31_RS22685 at 297.876 kb on - strand, within EX31_RS22685 at 297.912 kb on + strand, within EX31_RS22685 at 297.913 kb on - strand, within EX31_RS22685 at 297.915 kb on + strand, within EX31_RS22685 at 297.915 kb on + strand, within EX31_RS22685 at 297.916 kb on - strand, within EX31_RS22685 at 297.916 kb on - strand, within EX31_RS22685 at 297.961 kb on + strand at 297.961 kb on + strand at 297.962 kb on - strand at 297.975 kb on + strand at 297.975 kb on + strand at 298.003 kb on + strand at 298.003 kb on + strand at 298.003 kb on + strand at 298.003 kb on + strand at 298.004 kb on - strand at 298.004 kb on - strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.034 kb on + strand at 298.034 kb on + strand at 298.035 kb on - strand at 298.038 kb on + strand at 298.038 kb on + strand at 298.038 kb on + strand at 298.039 kb on - strand at 298.092 kb on + strand at 298.093 kb on - strand at 298.115 kb on + strand at 298.115 kb on + strand at 298.115 kb on + strand at 298.115 kb on + strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.181 kb on - strand at 298.181 kb on - strand at 298.183 kb on - strand at 298.183 kb on - strand at 298.204 kb on + strand at 298.210 kb on - strand at 298.337 kb on + strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.500 kb on + strand, within EX31_RS22690 at 298.573 kb on + strand, within EX31_RS22690 at 298.573 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.581 kb on + strand, within EX31_RS22690 at 298.582 kb on - strand, within EX31_RS22690 at 298.582 kb on - strand, within EX31_RS22690 at 298.592 kb on + strand, within EX31_RS22690 at 298.592 kb on + strand, within EX31_RS22690 at 298.593 kb on - strand, within EX31_RS22690 at 298.669 kb on + strand, within EX31_RS22690 at 298.669 kb on + strand, within EX31_RS22690 at 298.669 kb on + strand, within EX31_RS22690 at 298.670 kb on - strand, within EX31_RS22690 at 298.722 kb on + strand, within EX31_RS22690 at 298.722 kb on + strand, within EX31_RS22690 at 298.722 kb on + strand, within EX31_RS22690 at 298.735 kb on + strand, within EX31_RS22690 at 298.735 kb on + strand, within EX31_RS22690 at 298.736 kb on - strand, within EX31_RS22690 at 298.803 kb on - strand at 298.803 kb on - strand at 298.803 kb on - strand at 298.824 kb on - strand at 298.876 kb on - strand at 298.921 kb on + strand at 298.921 kb on + strand at 298.921 kb on + strand at 298.922 kb on - strand at 298.954 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 296,435 + EX31_RS22680 0.68 -0.5 296,436 - EX31_RS22680 0.68 -0.1 296,637 - EX31_RS22680 0.77 +1.4 296,642 + EX31_RS22680 0.78 -0.4 296,642 + EX31_RS22680 0.78 -0.5 296,643 - EX31_RS22680 0.78 -2.6 296,726 + EX31_RS22680 0.82 -0.5 296,932 + +0.6 296,932 + +2.0 296,952 + +0.2 296,955 - -0.3 296,955 - +0.7 296,978 + +0.2 296,978 + +1.0 296,979 - -0.5 296,979 - -0.4 296,979 - -0.7 297,068 - -0.3 297,068 - +0.3 297,068 - +0.1 297,068 - +0.5 297,082 - +0.1 297,082 - +1.3 297,083 + -1.9 297,120 - -0.4 297,138 + -0.0 297,138 + -0.8 297,139 - -1.2 297,139 - +0.2 297,139 - +0.8 297,170 + -0.1 297,170 + +1.1 297,170 + +1.5 297,170 + -1.1 297,171 - -0.2 297,171 - +0.9 297,171 - -2.5 297,171 - +0.7 297,171 - -0.0 297,171 - -0.8 297,180 + +0.7 297,180 + +0.6 297,180 + +0.2 297,181 - -0.3 297,181 - +0.8 297,181 - +0.3 297,181 - -0.2 297,181 - -0.6 297,181 - +0.2 297,204 - -0.3 297,244 + +0.2 297,245 - -0.8 297,251 + -0.3 297,251 + +1.0 297,251 + +1.1 297,252 - -0.4 297,252 - -0.5 297,252 - -0.8 297,252 - -0.5 297,254 + +0.2 297,255 - +1.6 297,255 - +0.3 297,291 + -0.0 297,291 + +0.2 297,292 - -1.3 297,292 - -0.9 297,292 - +0.9 297,292 - -0.2 297,292 - -2.5 297,292 - +0.1 297,292 - +0.3 297,292 - +2.3 297,385 - -0.9 297,414 - +0.5 297,498 + EX31_RS22685 0.15 -0.8 297,498 + EX31_RS22685 0.15 +0.7 297,499 - EX31_RS22685 0.15 -0.4 297,499 - EX31_RS22685 0.15 +0.2 297,522 - EX31_RS22685 0.19 -0.3 297,584 + EX31_RS22685 0.30 -0.2 297,585 - EX31_RS22685 0.30 +3.6 297,585 - EX31_RS22685 0.30 +0.0 297,622 + EX31_RS22685 0.36 -0.0 297,622 + EX31_RS22685 0.36 -0.3 297,623 - EX31_RS22685 0.37 +0.1 297,623 - EX31_RS22685 0.37 +2.1 297,627 - EX31_RS22685 0.37 +0.8 297,627 - EX31_RS22685 0.37 +0.2 297,627 - EX31_RS22685 0.37 +2.3 297,635 + EX31_RS22685 0.39 -0.2 297,636 - EX31_RS22685 0.39 +0.3 297,659 + EX31_RS22685 0.43 +0.8 297,660 - EX31_RS22685 0.43 -0.3 297,660 - EX31_RS22685 0.43 +0.8 297,750 + EX31_RS22685 0.59 -0.1 297,751 - EX31_RS22685 0.59 -0.0 297,751 - EX31_RS22685 0.59 +1.7 297,751 - EX31_RS22685 0.59 +1.6 297,751 - EX31_RS22685 0.59 +0.9 297,756 + EX31_RS22685 0.60 +1.6 297,757 - EX31_RS22685 0.60 +0.3 297,807 + EX31_RS22685 0.69 -0.7 297,807 + EX31_RS22685 0.69 +0.1 297,834 + EX31_RS22685 0.73 +0.7 297,835 - EX31_RS22685 0.73 -2.9 297,835 - EX31_RS22685 0.73 +0.9 297,835 - EX31_RS22685 0.73 +0.6 297,835 - EX31_RS22685 0.73 +0.0 297,835 - EX31_RS22685 0.73 +1.1 297,845 + EX31_RS22685 0.75 -0.1 297,845 + EX31_RS22685 0.75 +1.5 297,845 + EX31_RS22685 0.75 +0.4 297,846 - EX31_RS22685 0.75 -0.3 297,875 + EX31_RS22685 0.80 -0.1 297,876 - EX31_RS22685 0.81 -0.0 297,912 + EX31_RS22685 0.87 -0.0 297,913 - EX31_RS22685 0.87 +0.0 297,915 + EX31_RS22685 0.87 -1.0 297,915 + EX31_RS22685 0.87 +1.5 297,916 - EX31_RS22685 0.88 +1.2 297,916 - EX31_RS22685 0.88 -0.3 297,961 + -0.6 297,961 + +0.3 297,962 - +0.5 297,975 + +0.1 297,975 + -1.8 298,003 + +0.1 298,003 + +1.4 298,003 + -0.5 298,003 + +0.5 298,004 - -0.1 298,004 - -1.0 298,005 + +0.7 298,005 + -1.6 298,005 + -0.2 298,005 + +0.3 298,005 + -0.1 298,005 + +0.4 298,005 + +0.5 298,006 - +0.1 298,006 - -0.3 298,006 - -0.4 298,006 - +0.3 298,006 - +0.9 298,006 - -1.8 298,006 - -0.5 298,006 - -0.6 298,034 + +0.3 298,034 + +0.2 298,035 - -1.4 298,038 + +1.1 298,038 + +0.8 298,038 + -1.2 298,039 - +1.5 298,092 + -0.6 298,093 - +0.4 298,115 + -0.2 298,115 + +0.5 298,115 + -0.8 298,115 + +1.6 298,116 - -0.1 298,116 - -1.0 298,116 - -0.0 298,116 - -0.8 298,116 - -0.3 298,181 - -3.0 298,181 - +0.1 298,183 - +0.7 298,183 - -0.1 298,204 + +1.3 298,210 - +0.8 298,337 + EX31_RS22690 0.20 +1.8 298,338 - EX31_RS22690 0.20 +0.1 298,338 - EX31_RS22690 0.20 -0.6 298,338 - EX31_RS22690 0.20 -0.7 298,338 - EX31_RS22690 0.20 -0.3 298,338 - EX31_RS22690 0.20 -0.2 298,500 + EX31_RS22690 0.46 -0.0 298,573 + EX31_RS22690 0.57 +1.6 298,573 + EX31_RS22690 0.57 -0.4 298,579 + EX31_RS22690 0.58 -2.1 298,579 + EX31_RS22690 0.58 +1.5 298,579 + EX31_RS22690 0.58 -2.8 298,579 + EX31_RS22690 0.58 -1.7 298,579 + EX31_RS22690 0.58 -1.0 298,579 + EX31_RS22690 0.58 -0.6 298,579 + EX31_RS22690 0.58 -1.2 298,579 + EX31_RS22690 0.58 -0.9 298,579 + EX31_RS22690 0.58 -0.4 298,579 + EX31_RS22690 0.58 +2.0 298,579 + EX31_RS22690 0.58 +1.0 298,579 + EX31_RS22690 0.58 -1.4 298,579 + EX31_RS22690 0.58 -0.9 298,579 + EX31_RS22690 0.58 -0.5 298,579 + EX31_RS22690 0.58 +0.4 298,579 + EX31_RS22690 0.58 -1.0 298,580 - EX31_RS22690 0.58 +0.6 298,580 - EX31_RS22690 0.58 -0.5 298,580 - EX31_RS22690 0.58 -0.8 298,580 - EX31_RS22690 0.58 -0.0 298,580 - EX31_RS22690 0.58 +0.3 298,580 - EX31_RS22690 0.58 -0.3 298,580 - EX31_RS22690 0.58 -0.0 298,580 - EX31_RS22690 0.58 -3.1 298,580 - EX31_RS22690 0.58 -1.3 298,580 - EX31_RS22690 0.58 +0.6 298,580 - EX31_RS22690 0.58 +0.6 298,580 - EX31_RS22690 0.58 -1.8 298,580 - EX31_RS22690 0.58 +0.4 298,580 - EX31_RS22690 0.58 -1.5 298,580 - EX31_RS22690 0.58 -2.1 298,581 + EX31_RS22690 0.58 -0.3 298,582 - EX31_RS22690 0.59 -0.1 298,582 - EX31_RS22690 0.59 -1.1 298,592 + EX31_RS22690 0.60 +0.9 298,592 + EX31_RS22690 0.60 +1.3 298,593 - EX31_RS22690 0.60 -0.2 298,669 + EX31_RS22690 0.72 -0.0 298,669 + EX31_RS22690 0.72 -1.3 298,669 + EX31_RS22690 0.72 +0.3 298,670 - EX31_RS22690 0.72 -0.4 298,722 + EX31_RS22690 0.80 -0.8 298,722 + EX31_RS22690 0.80 -0.5 298,722 + EX31_RS22690 0.80 -0.9 298,735 + EX31_RS22690 0.82 -0.2 298,735 + EX31_RS22690 0.82 -1.2 298,736 - EX31_RS22690 0.83 -1.6 298,803 - -1.5 298,803 - +0.4 298,803 - +1.3 298,824 - +0.4 298,876 - -0.3 298,921 + +1.2 298,921 + -2.5 298,921 + -0.0 298,922 - -0.5 298,954 + -3.4
Or see this region's nucleotide sequence