Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22080 and EX31_RS22085 are separated by 225 nucleotides EX31_RS22085 and EX31_RS22090 are separated by 134 nucleotides
EX31_RS22080: EX31_RS22080 - peroxiredoxin C, at 165,107 to 165,709
_RS22080
EX31_RS22085: EX31_RS22085 - DUF479 domain-containing protein, at 165,935 to 166,516
_RS22085
EX31_RS22090: EX31_RS22090 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 166,651 to 167,721
_RS22090
Position (kb) 165
166
167 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 164.961 kb on - strand at 164.995 kb on + strand at 164.995 kb on + strand at 164.995 kb on + strand at 164.996 kb on - strand at 165.135 kb on + strand at 165.136 kb on - strand at 165.136 kb on - strand at 165.140 kb on - strand at 165.369 kb on + strand, within EX31_RS22080 at 165.370 kb on - strand, within EX31_RS22080 at 165.370 kb on - strand, within EX31_RS22080 at 165.412 kb on - strand, within EX31_RS22080 at 165.412 kb on - strand, within EX31_RS22080 at 165.441 kb on - strand, within EX31_RS22080 at 165.452 kb on - strand, within EX31_RS22080 at 165.495 kb on - strand, within EX31_RS22080 at 165.504 kb on - strand, within EX31_RS22080 at 165.504 kb on - strand, within EX31_RS22080 at 165.596 kb on - strand, within EX31_RS22080 at 165.596 kb on - strand, within EX31_RS22080 at 165.596 kb on - strand, within EX31_RS22080 at 165.601 kb on + strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.602 kb on - strand, within EX31_RS22080 at 165.645 kb on - strand, within EX31_RS22080 at 165.645 kb on - strand, within EX31_RS22080 at 165.699 kb on - strand at 165.709 kb on + strand at 165.710 kb on - strand at 165.712 kb on + strand at 165.712 kb on + strand at 165.712 kb on + strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.713 kb on - strand at 165.715 kb on + strand at 165.715 kb on + strand at 165.716 kb on - strand at 165.716 kb on - strand at 165.716 kb on - strand at 165.716 kb on - strand at 165.716 kb on - strand at 165.744 kb on + strand at 165.744 kb on + strand at 165.745 kb on - strand at 165.745 kb on - strand at 165.745 kb on - strand at 165.745 kb on - strand at 165.781 kb on + strand at 165.782 kb on - strand at 165.784 kb on - strand at 165.784 kb on - strand at 165.784 kb on - strand at 165.804 kb on + strand at 165.805 kb on - strand at 165.805 kb on - strand at 165.832 kb on - strand at 165.857 kb on + strand at 165.857 kb on + strand at 165.857 kb on + strand at 165.858 kb on - strand at 165.858 kb on - strand at 165.858 kb on - strand at 165.881 kb on - strand at 165.881 kb on - strand at 165.881 kb on - strand at 165.911 kb on - strand at 165.911 kb on - strand at 165.911 kb on - strand at 165.911 kb on - strand at 165.929 kb on + strand at 165.929 kb on + strand at 165.929 kb on + strand at 165.929 kb on + strand at 165.930 kb on - strand at 165.930 kb on - strand at 165.930 kb on - strand at 166.008 kb on + strand, within EX31_RS22085 at 166.008 kb on + strand, within EX31_RS22085 at 166.008 kb on + strand, within EX31_RS22085 at 166.009 kb on - strand, within EX31_RS22085 at 166.009 kb on - strand, within EX31_RS22085 at 166.021 kb on + strand, within EX31_RS22085 at 166.083 kb on - strand, within EX31_RS22085 at 166.083 kb on - strand, within EX31_RS22085 at 166.083 kb on - strand, within EX31_RS22085 at 166.140 kb on + strand, within EX31_RS22085 at 166.141 kb on - strand, within EX31_RS22085 at 166.141 kb on - strand, within EX31_RS22085 at 166.326 kb on + strand, within EX31_RS22085 at 166.326 kb on + strand, within EX31_RS22085 at 166.326 kb on + strand, within EX31_RS22085 at 166.326 kb on + strand, within EX31_RS22085 at 166.327 kb on - strand, within EX31_RS22085 at 166.327 kb on - strand, within EX31_RS22085 at 166.327 kb on - strand, within EX31_RS22085 at 166.525 kb on + strand at 166.525 kb on + strand at 166.525 kb on + strand at 166.525 kb on + strand at 166.525 kb on + strand at 166.525 kb on + strand at 166.525 kb on + strand at 166.525 kb on + strand at 166.526 kb on - strand at 166.526 kb on - strand at 166.526 kb on - strand at 166.526 kb on - strand at 166.526 kb on - strand at 166.527 kb on + strand at 166.527 kb on + strand at 166.527 kb on + strand at 166.528 kb on - strand at 166.528 kb on - strand at 166.612 kb on + strand at 166.612 kb on + strand at 166.612 kb on + strand at 166.613 kb on - strand at 166.633 kb on + strand at 166.633 kb on + strand at 166.633 kb on + strand at 166.763 kb on + strand, within EX31_RS22090 at 166.763 kb on + strand, within EX31_RS22090 at 166.820 kb on + strand, within EX31_RS22090 at 166.820 kb on + strand, within EX31_RS22090 at 166.820 kb on + strand, within EX31_RS22090 at 166.824 kb on + strand, within EX31_RS22090 at 166.824 kb on + strand, within EX31_RS22090 at 166.983 kb on + strand, within EX31_RS22090 at 166.992 kb on + strand, within EX31_RS22090 at 166.992 kb on + strand, within EX31_RS22090 at 166.992 kb on + strand, within EX31_RS22090 at 166.993 kb on - strand, within EX31_RS22090 at 166.995 kb on + strand, within EX31_RS22090 at 166.995 kb on + strand, within EX31_RS22090 at 167.022 kb on + strand, within EX31_RS22090 at 167.022 kb on + strand, within EX31_RS22090 at 167.022 kb on + strand, within EX31_RS22090 at 167.022 kb on + strand, within EX31_RS22090 at 167.022 kb on + strand, within EX31_RS22090 at 167.022 kb on + strand, within EX31_RS22090 at 167.064 kb on + strand, within EX31_RS22090 at 167.088 kb on + strand, within EX31_RS22090 at 167.104 kb on + strand, within EX31_RS22090 at 167.104 kb on + strand, within EX31_RS22090 at 167.106 kb on + strand, within EX31_RS22090 at 167.106 kb on + strand, within EX31_RS22090 at 167.106 kb on + strand, within EX31_RS22090 at 167.106 kb on + strand, within EX31_RS22090 at 167.143 kb on + strand, within EX31_RS22090 at 167.143 kb on + strand, within EX31_RS22090 at 167.143 kb on + strand, within EX31_RS22090 at 167.143 kb on + strand, within EX31_RS22090 at 167.143 kb on + strand, within EX31_RS22090 at 167.155 kb on + strand, within EX31_RS22090 at 167.394 kb on + strand, within EX31_RS22090 at 167.402 kb on + strand, within EX31_RS22090 at 167.402 kb on + strand, within EX31_RS22090 at 167.403 kb on - strand, within EX31_RS22090 at 167.504 kb on - strand, within EX31_RS22090
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 164,961 - +0.1 164,995 + -1.8 164,995 + +1.5 164,995 + +1.6 164,996 - +0.8 165,135 + +1.3 165,136 - +0.2 165,136 - -0.2 165,140 - +0.1 165,369 + EX31_RS22080 0.43 +1.0 165,370 - EX31_RS22080 0.44 +1.0 165,370 - EX31_RS22080 0.44 +1.7 165,412 - EX31_RS22080 0.51 -0.2 165,412 - EX31_RS22080 0.51 +0.3 165,441 - EX31_RS22080 0.55 +0.5 165,452 - EX31_RS22080 0.57 +0.3 165,495 - EX31_RS22080 0.64 +0.3 165,504 - EX31_RS22080 0.66 -0.2 165,504 - EX31_RS22080 0.66 -0.6 165,596 - EX31_RS22080 0.81 +0.7 165,596 - EX31_RS22080 0.81 -0.4 165,596 - EX31_RS22080 0.81 +0.1 165,601 + EX31_RS22080 0.82 +1.7 165,602 - EX31_RS22080 0.82 -1.9 165,602 - EX31_RS22080 0.82 -0.7 165,602 - EX31_RS22080 0.82 -0.8 165,602 - EX31_RS22080 0.82 -0.5 165,602 - EX31_RS22080 0.82 -0.9 165,602 - EX31_RS22080 0.82 -0.4 165,602 - EX31_RS22080 0.82 -0.8 165,602 - EX31_RS22080 0.82 -0.8 165,645 - EX31_RS22080 0.89 +0.5 165,645 - EX31_RS22080 0.89 +1.0 165,699 - -0.5 165,709 + -0.2 165,710 - -0.2 165,712 + -0.3 165,712 + +0.4 165,712 + +0.1 165,713 - +2.0 165,713 - +0.2 165,713 - -0.8 165,713 - -0.2 165,713 - +0.1 165,713 - -0.8 165,713 - -0.3 165,713 - +0.4 165,713 - +0.1 165,713 - -1.4 165,713 - +0.4 165,713 - +0.8 165,715 + +0.0 165,715 + -0.7 165,716 - +0.0 165,716 - -0.5 165,716 - +0.5 165,716 - +0.7 165,716 - +0.9 165,744 + +0.0 165,744 + +0.6 165,745 - +0.3 165,745 - -0.1 165,745 - -1.1 165,745 - +0.3 165,781 + -0.4 165,782 - +0.7 165,784 - +0.0 165,784 - -0.1 165,784 - -0.7 165,804 + +0.1 165,805 - -0.5 165,805 - +0.8 165,832 - -1.3 165,857 + -0.3 165,857 + -0.5 165,857 + -0.4 165,858 - -0.4 165,858 - +0.8 165,858 - -0.2 165,881 - +0.1 165,881 - -0.5 165,881 - +0.2 165,911 - -0.1 165,911 - -0.8 165,911 - +0.5 165,911 - -0.4 165,929 + +1.4 165,929 + +1.0 165,929 + -1.8 165,929 + -1.5 165,930 - +0.5 165,930 - -0.2 165,930 - -0.8 166,008 + EX31_RS22085 0.13 +2.0 166,008 + EX31_RS22085 0.13 +0.2 166,008 + EX31_RS22085 0.13 -0.3 166,009 - EX31_RS22085 0.13 -1.4 166,009 - EX31_RS22085 0.13 -0.4 166,021 + EX31_RS22085 0.15 +0.8 166,083 - EX31_RS22085 0.25 -1.3 166,083 - EX31_RS22085 0.25 -2.1 166,083 - EX31_RS22085 0.25 +0.3 166,140 + EX31_RS22085 0.35 -0.2 166,141 - EX31_RS22085 0.35 -0.3 166,141 - EX31_RS22085 0.35 -1.0 166,326 + EX31_RS22085 0.67 -0.5 166,326 + EX31_RS22085 0.67 -0.3 166,326 + EX31_RS22085 0.67 +0.9 166,326 + EX31_RS22085 0.67 -0.4 166,327 - EX31_RS22085 0.67 +0.6 166,327 - EX31_RS22085 0.67 +0.2 166,327 - EX31_RS22085 0.67 -0.1 166,525 + +0.4 166,525 + +0.2 166,525 + -0.9 166,525 + -1.4 166,525 + -0.4 166,525 + -0.5 166,525 + -0.8 166,525 + -0.2 166,526 - -0.7 166,526 - -0.6 166,526 - -1.0 166,526 - -0.3 166,526 - +0.2 166,527 + -1.9 166,527 + -0.2 166,527 + -0.9 166,528 - +0.7 166,528 - -0.5 166,612 + -0.3 166,612 + +0.0 166,612 + -0.2 166,613 - +2.0 166,633 + +0.7 166,633 + +0.1 166,633 + -0.2 166,763 + EX31_RS22090 0.10 -0.3 166,763 + EX31_RS22090 0.10 -1.1 166,820 + EX31_RS22090 0.16 -0.4 166,820 + EX31_RS22090 0.16 +0.3 166,820 + EX31_RS22090 0.16 -0.2 166,824 + EX31_RS22090 0.16 -0.4 166,824 + EX31_RS22090 0.16 +0.1 166,983 + EX31_RS22090 0.31 +0.7 166,992 + EX31_RS22090 0.32 +0.3 166,992 + EX31_RS22090 0.32 +0.5 166,992 + EX31_RS22090 0.32 -0.2 166,993 - EX31_RS22090 0.32 +0.8 166,995 + EX31_RS22090 0.32 +0.0 166,995 + EX31_RS22090 0.32 +0.2 167,022 + EX31_RS22090 0.35 -0.8 167,022 + EX31_RS22090 0.35 +0.3 167,022 + EX31_RS22090 0.35 +0.1 167,022 + EX31_RS22090 0.35 +0.5 167,022 + EX31_RS22090 0.35 -1.0 167,022 + EX31_RS22090 0.35 -0.5 167,064 + EX31_RS22090 0.39 -1.2 167,088 + EX31_RS22090 0.41 -0.2 167,104 + EX31_RS22090 0.42 -0.7 167,104 + EX31_RS22090 0.42 -0.4 167,106 + EX31_RS22090 0.42 +0.1 167,106 + EX31_RS22090 0.42 -0.3 167,106 + EX31_RS22090 0.42 -0.5 167,106 + EX31_RS22090 0.42 -0.7 167,143 + EX31_RS22090 0.46 +0.3 167,143 + EX31_RS22090 0.46 -0.4 167,143 + EX31_RS22090 0.46 +0.5 167,143 + EX31_RS22090 0.46 -0.6 167,143 + EX31_RS22090 0.46 -0.5 167,155 + EX31_RS22090 0.47 -1.0 167,394 + EX31_RS22090 0.69 +0.6 167,402 + EX31_RS22090 0.70 -0.3 167,402 + EX31_RS22090 0.70 -0.7 167,403 - EX31_RS22090 0.70 -1.8 167,504 - EX31_RS22090 0.80 +3.0
Or see this region's nucleotide sequence