Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21975 and EX31_RS21980 are separated by 4 nucleotides EX31_RS21980 and EX31_RS21985 are separated by 179 nucleotides EX31_RS21985 and EX31_RS21990 are separated by 58 nucleotides
EX31_RS21975: EX31_RS21975 - DUF1177 domain-containing protein, at 139,783 to 140,712
_RS21975
EX31_RS21980: EX31_RS21980 - AroM family protein, at 140,717 to 141,394
_RS21980
EX31_RS21985: EX31_RS21985 - pyrimidine/purine nucleoside phosphorylase, at 141,574 to 141,864
_RS21985
EX31_RS21990: EX31_RS21990 - recombination-associated protein RdgC, at 141,923 to 142,837
_RS21990
Position (kb) 141
142 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 140.656 kb on + strand at 140.678 kb on - strand at 140.710 kb on + strand at 140.771 kb on - strand at 140.824 kb on - strand, within EX31_RS21980 at 140.850 kb on + strand, within EX31_RS21980 at 140.850 kb on + strand, within EX31_RS21980 at 140.850 kb on + strand, within EX31_RS21980 at 140.850 kb on + strand, within EX31_RS21980 at 140.851 kb on - strand, within EX31_RS21980 at 140.886 kb on + strand, within EX31_RS21980 at 140.920 kb on + strand, within EX31_RS21980 at 140.921 kb on - strand, within EX31_RS21980 at 140.953 kb on - strand, within EX31_RS21980 at 141.037 kb on + strand, within EX31_RS21980 at 141.037 kb on + strand, within EX31_RS21980 at 141.038 kb on - strand, within EX31_RS21980 at 141.038 kb on - strand, within EX31_RS21980 at 141.070 kb on + strand, within EX31_RS21980 at 141.071 kb on - strand, within EX31_RS21980 at 141.093 kb on + strand, within EX31_RS21980 at 141.334 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.505 kb on - strand at 141.534 kb on + strand at 141.537 kb on + strand at 141.538 kb on - strand at 141.538 kb on - strand at 141.538 kb on - strand at 141.538 kb on - strand at 141.573 kb on + strand at 141.573 kb on + strand at 141.574 kb on - strand at 141.609 kb on - strand, within EX31_RS21985 at 141.676 kb on + strand, within EX31_RS21985 at 141.678 kb on + strand, within EX31_RS21985 at 141.679 kb on - strand, within EX31_RS21985 at 141.768 kb on + strand, within EX31_RS21985 at 141.769 kb on - strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.788 kb on - strand, within EX31_RS21985 at 141.788 kb on - strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.839 kb on - strand at 141.839 kb on - strand at 141.839 kb on - strand at 141.839 kb on - strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.851 kb on - strand at 141.851 kb on - strand at 141.851 kb on - strand at 141.851 kb on - strand at 141.858 kb on + strand at 141.858 kb on + strand at 141.859 kb on - strand at 141.862 kb on + strand at 141.862 kb on + strand at 141.862 kb on + strand at 141.862 kb on + strand at 141.944 kb on + strand at 141.944 kb on + strand at 141.945 kb on - strand at 141.945 kb on - strand at 142.039 kb on + strand, within EX31_RS21990 at 142.085 kb on - strand, within EX31_RS21990 at 142.117 kb on + strand, within EX31_RS21990 at 142.118 kb on - strand, within EX31_RS21990 at 142.144 kb on + strand, within EX31_RS21990 at 142.144 kb on + strand, within EX31_RS21990 at 142.144 kb on + strand, within EX31_RS21990 at 142.145 kb on - strand, within EX31_RS21990 at 142.145 kb on - strand, within EX31_RS21990 at 142.145 kb on - strand, within EX31_RS21990 at 142.208 kb on - strand, within EX31_RS21990 at 142.268 kb on - strand, within EX31_RS21990 at 142.369 kb on + strand, within EX31_RS21990 at 142.583 kb on + strand, within EX31_RS21990 at 142.584 kb on - strand, within EX31_RS21990 at 142.594 kb on + strand, within EX31_RS21990 at 142.594 kb on + strand, within EX31_RS21990 at 142.595 kb on - strand, within EX31_RS21990 at 142.832 kb on + strand at 142.832 kb on + strand at 142.833 kb on - strand at 142.833 kb on - strand at 142.833 kb on - strand at 142.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 140,656 + -0.4 140,678 - -0.3 140,710 + -0.1 140,771 - +1.0 140,824 - EX31_RS21980 0.16 +1.3 140,850 + EX31_RS21980 0.20 +0.7 140,850 + EX31_RS21980 0.20 -0.3 140,850 + EX31_RS21980 0.20 -2.1 140,850 + EX31_RS21980 0.20 -0.1 140,851 - EX31_RS21980 0.20 -1.4 140,886 + EX31_RS21980 0.25 -0.4 140,920 + EX31_RS21980 0.30 -0.6 140,921 - EX31_RS21980 0.30 -0.1 140,953 - EX31_RS21980 0.35 -1.0 141,037 + EX31_RS21980 0.47 +0.4 141,037 + EX31_RS21980 0.47 +0.3 141,038 - EX31_RS21980 0.47 -0.4 141,038 - EX31_RS21980 0.47 -1.3 141,070 + EX31_RS21980 0.52 -0.2 141,071 - EX31_RS21980 0.52 +0.3 141,093 + EX31_RS21980 0.55 +0.3 141,334 + -2.7 141,357 + -0.1 141,357 + +2.0 141,357 + -0.6 141,357 + -0.8 141,357 + +0.7 141,357 + +0.5 141,358 - -0.1 141,358 - +0.0 141,358 - +0.8 141,358 - +0.6 141,358 - -0.1 141,504 + +1.2 141,504 + -2.1 141,504 + -0.1 141,504 + -0.5 141,504 + +0.0 141,505 - +1.4 141,534 + -1.0 141,537 + -0.1 141,538 - +0.8 141,538 - +0.5 141,538 - -2.0 141,538 - +0.6 141,573 + -0.1 141,573 + +0.4 141,574 - -2.0 141,609 - EX31_RS21985 0.12 -0.4 141,676 + EX31_RS21985 0.35 +0.4 141,678 + EX31_RS21985 0.36 +1.0 141,679 - EX31_RS21985 0.36 +0.1 141,768 + EX31_RS21985 0.67 -0.4 141,769 - EX31_RS21985 0.67 +0.5 141,787 + EX31_RS21985 0.73 +0.5 141,787 + EX31_RS21985 0.73 +0.7 141,787 + EX31_RS21985 0.73 +0.6 141,787 + EX31_RS21985 0.73 -0.2 141,788 - EX31_RS21985 0.74 -0.1 141,788 - EX31_RS21985 0.74 -0.0 141,815 + EX31_RS21985 0.83 +0.5 141,815 + EX31_RS21985 0.83 +0.8 141,815 + EX31_RS21985 0.83 -0.0 141,815 + EX31_RS21985 0.83 +1.5 141,838 + -1.4 141,838 + +1.5 141,838 + +0.5 141,838 + -1.5 141,838 + +1.0 141,838 + +0.3 141,838 + -0.1 141,839 - -0.5 141,839 - -1.1 141,839 - +0.5 141,839 - -0.2 141,846 + -0.2 141,846 + -0.3 141,846 + +0.1 141,847 - +1.0 141,847 - +1.0 141,847 - +2.8 141,847 - +1.5 141,847 - -0.8 141,850 + -0.5 141,850 + +0.1 141,850 + +2.0 141,850 + +0.4 141,850 + -0.9 141,851 - +0.5 141,851 - +0.3 141,851 - +1.5 141,851 - -2.0 141,858 + +0.9 141,858 + +0.7 141,859 - -1.2 141,862 + +0.0 141,862 + +0.8 141,862 + +1.0 141,862 + +0.0 141,944 + -0.8 141,944 + -0.8 141,945 - +0.7 141,945 - +2.6 142,039 + EX31_RS21990 0.13 -1.8 142,085 - EX31_RS21990 0.18 +0.5 142,117 + EX31_RS21990 0.21 +0.1 142,118 - EX31_RS21990 0.21 -0.4 142,144 + EX31_RS21990 0.24 +0.9 142,144 + EX31_RS21990 0.24 +0.8 142,144 + EX31_RS21990 0.24 +0.9 142,145 - EX31_RS21990 0.24 +1.8 142,145 - EX31_RS21990 0.24 +0.4 142,145 - EX31_RS21990 0.24 +0.3 142,208 - EX31_RS21990 0.31 -0.8 142,268 - EX31_RS21990 0.38 -0.0 142,369 + EX31_RS21990 0.49 +0.4 142,583 + EX31_RS21990 0.72 -0.7 142,584 - EX31_RS21990 0.72 +0.8 142,594 + EX31_RS21990 0.73 +0.8 142,594 + EX31_RS21990 0.73 -0.5 142,595 - EX31_RS21990 0.73 -0.7 142,832 + -0.5 142,832 + +1.2 142,833 - -1.4 142,833 - -1.9 142,833 - +0.1 142,833 - -0.1
Or see this region's nucleotide sequence