Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18710 and EX31_RS18715 are separated by 43 nucleotides EX31_RS18715 and EX31_RS18720 are separated by 11 nucleotides EX31_RS18720 and EX31_RS18725 are separated by 63 nucleotides
EX31_RS18710: EX31_RS18710 - LysR family transcriptional regulator, at 322,903 to 323,832
_RS18710
EX31_RS18715: EX31_RS18715 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 323,876 to 325,000
_RS18715
EX31_RS18720: EX31_RS18720 - S-formylglutathione hydrolase, at 325,012 to 325,869
_RS18720
EX31_RS18725: EX31_RS18725 - glutathione ABC transporter permease GsiD, at 325,933 to 326,853
_RS18725
Position (kb) 323
324
325
326 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 323.101 kb on + strand, within EX31_RS18710 at 323.101 kb on + strand, within EX31_RS18710 at 323.101 kb on + strand, within EX31_RS18710 at 323.102 kb on - strand, within EX31_RS18710 at 323.233 kb on + strand, within EX31_RS18710 at 323.233 kb on + strand, within EX31_RS18710 at 323.233 kb on + strand, within EX31_RS18710 at 323.233 kb on + strand, within EX31_RS18710 at 323.233 kb on + strand, within EX31_RS18710 at 323.358 kb on + strand, within EX31_RS18710 at 323.358 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.370 kb on + strand, within EX31_RS18710 at 323.694 kb on + strand, within EX31_RS18710 at 323.694 kb on + strand, within EX31_RS18710 at 323.827 kb on + strand at 323.827 kb on + strand at 323.827 kb on + strand at 323.827 kb on + strand at 323.827 kb on + strand at 323.827 kb on + strand at 323.827 kb on + strand at 323.828 kb on - strand at 323.828 kb on - strand at 323.830 kb on + strand at 323.830 kb on + strand at 323.831 kb on - strand at 323.835 kb on + strand at 323.835 kb on + strand at 323.836 kb on - strand at 323.847 kb on - strand at 323.850 kb on + strand at 323.850 kb on + strand at 323.850 kb on + strand at 323.850 kb on + strand at 323.851 kb on - strand at 323.851 kb on - strand at 323.870 kb on + strand at 323.983 kb on + strand at 324.007 kb on + strand, within EX31_RS18715 at 324.007 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.017 kb on + strand, within EX31_RS18715 at 324.018 kb on - strand, within EX31_RS18715 at 324.146 kb on + strand, within EX31_RS18715 at 324.146 kb on + strand, within EX31_RS18715 at 324.146 kb on + strand, within EX31_RS18715 at 324.147 kb on - strand, within EX31_RS18715 at 324.169 kb on + strand, within EX31_RS18715 at 324.169 kb on + strand, within EX31_RS18715 at 324.169 kb on + strand, within EX31_RS18715 at 324.169 kb on + strand, within EX31_RS18715 at 324.169 kb on + strand, within EX31_RS18715 at 324.169 kb on + strand, within EX31_RS18715 at 324.170 kb on - strand, within EX31_RS18715 at 324.227 kb on - strand, within EX31_RS18715 at 324.227 kb on - strand, within EX31_RS18715 at 324.287 kb on + strand, within EX31_RS18715 at 324.287 kb on + strand, within EX31_RS18715 at 324.287 kb on + strand, within EX31_RS18715 at 324.287 kb on + strand, within EX31_RS18715 at 324.287 kb on + strand, within EX31_RS18715 at 324.288 kb on - strand, within EX31_RS18715 at 324.288 kb on - strand, within EX31_RS18715 at 324.288 kb on - strand, within EX31_RS18715 at 324.314 kb on + strand, within EX31_RS18715 at 324.673 kb on + strand, within EX31_RS18715 at 324.673 kb on + strand, within EX31_RS18715 at 324.674 kb on - strand, within EX31_RS18715 at 324.674 kb on - strand, within EX31_RS18715 at 324.872 kb on + strand, within EX31_RS18715 at 324.873 kb on - strand, within EX31_RS18715 at 324.970 kb on + strand at 324.970 kb on + strand at 324.971 kb on - strand at 324.991 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.998 kb on + strand at 324.998 kb on + strand at 324.998 kb on + strand at 324.998 kb on + strand at 324.999 kb on - strand at 324.999 kb on - strand at 324.999 kb on - strand at 324.999 kb on - strand at 324.999 kb on - strand at 325.031 kb on + strand at 325.031 kb on + strand at 325.031 kb on + strand at 325.032 kb on - strand at 325.080 kb on + strand at 325.081 kb on - strand at 325.081 kb on - strand at 325.120 kb on + strand, within EX31_RS18720 at 325.120 kb on + strand, within EX31_RS18720 at 325.121 kb on - strand, within EX31_RS18720 at 325.121 kb on - strand, within EX31_RS18720 at 325.162 kb on + strand, within EX31_RS18720 at 325.185 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.376 kb on - strand, within EX31_RS18720 at 325.376 kb on - strand, within EX31_RS18720 at 325.381 kb on + strand, within EX31_RS18720 at 325.381 kb on + strand, within EX31_RS18720 at 325.566 kb on + strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.583 kb on - strand, within EX31_RS18720 at 325.611 kb on - strand, within EX31_RS18720 at 325.615 kb on + strand, within EX31_RS18720 at 325.615 kb on + strand, within EX31_RS18720 at 325.715 kb on + strand, within EX31_RS18720 at 325.715 kb on + strand, within EX31_RS18720 at 325.715 kb on + strand, within EX31_RS18720 at 325.716 kb on - strand, within EX31_RS18720 at 325.787 kb on - strand at 325.798 kb on + strand at 325.799 kb on - strand at 325.803 kb on + strand at 325.803 kb on + strand at 325.804 kb on - strand at 325.881 kb on + strand at 325.881 kb on + strand at 325.881 kb on + strand at 325.887 kb on + strand at 325.887 kb on + strand at 325.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 323,101 + EX31_RS18710 0.21 +0.4 323,101 + EX31_RS18710 0.21 +1.0 323,101 + EX31_RS18710 0.21 -0.7 323,102 - EX31_RS18710 0.21 +1.0 323,233 + EX31_RS18710 0.35 +0.1 323,233 + EX31_RS18710 0.35 +0.7 323,233 + EX31_RS18710 0.35 +1.0 323,233 + EX31_RS18710 0.35 -1.1 323,233 + EX31_RS18710 0.35 -0.5 323,358 + EX31_RS18710 0.49 +0.1 323,358 + EX31_RS18710 0.49 -2.7 323,370 + EX31_RS18710 0.50 +0.2 323,370 + EX31_RS18710 0.50 +0.1 323,370 + EX31_RS18710 0.50 +0.0 323,370 + EX31_RS18710 0.50 -0.5 323,370 + EX31_RS18710 0.50 +0.3 323,370 + EX31_RS18710 0.50 -0.0 323,370 + EX31_RS18710 0.50 +1.4 323,694 + EX31_RS18710 0.85 +1.2 323,694 + EX31_RS18710 0.85 -0.6 323,827 + +1.2 323,827 + +0.2 323,827 + +1.0 323,827 + -0.1 323,827 + -0.6 323,827 + -1.2 323,827 + -0.0 323,828 - +3.0 323,828 - +0.2 323,830 + +0.4 323,830 + -0.6 323,831 - +0.9 323,835 + +0.5 323,835 + +0.0 323,836 - -0.7 323,847 - +0.5 323,850 + +0.2 323,850 + -1.3 323,850 + -0.6 323,850 + -0.3 323,851 - -0.7 323,851 - -1.3 323,870 + +0.1 323,983 + +0.4 324,007 + EX31_RS18715 0.12 -2.1 324,007 + EX31_RS18715 0.12 +0.6 324,017 + EX31_RS18715 0.13 -0.5 324,017 + EX31_RS18715 0.13 +1.2 324,017 + EX31_RS18715 0.13 +0.3 324,017 + EX31_RS18715 0.13 +0.4 324,017 + EX31_RS18715 0.13 +0.8 324,017 + EX31_RS18715 0.13 -0.4 324,017 + EX31_RS18715 0.13 -1.7 324,017 + EX31_RS18715 0.13 +0.5 324,018 - EX31_RS18715 0.13 -0.4 324,146 + EX31_RS18715 0.24 +0.4 324,146 + EX31_RS18715 0.24 +1.2 324,146 + EX31_RS18715 0.24 -1.6 324,147 - EX31_RS18715 0.24 -0.5 324,169 + EX31_RS18715 0.26 -1.7 324,169 + EX31_RS18715 0.26 -0.3 324,169 + EX31_RS18715 0.26 +0.0 324,169 + EX31_RS18715 0.26 +0.4 324,169 + EX31_RS18715 0.26 -0.7 324,169 + EX31_RS18715 0.26 -0.1 324,170 - EX31_RS18715 0.26 -0.6 324,227 - EX31_RS18715 0.31 +1.2 324,227 - EX31_RS18715 0.31 +0.2 324,287 + EX31_RS18715 0.37 -0.8 324,287 + EX31_RS18715 0.37 +0.4 324,287 + EX31_RS18715 0.37 +0.4 324,287 + EX31_RS18715 0.37 -1.1 324,287 + EX31_RS18715 0.37 +1.0 324,288 - EX31_RS18715 0.37 -0.8 324,288 - EX31_RS18715 0.37 -0.7 324,288 - EX31_RS18715 0.37 -1.6 324,314 + EX31_RS18715 0.39 +0.4 324,673 + EX31_RS18715 0.71 +0.4 324,673 + EX31_RS18715 0.71 -0.1 324,674 - EX31_RS18715 0.71 +1.3 324,674 - EX31_RS18715 0.71 +0.0 324,872 + EX31_RS18715 0.89 +0.0 324,873 - EX31_RS18715 0.89 +0.6 324,970 + +1.4 324,970 + +1.2 324,971 - -0.4 324,991 + -0.4 324,994 + -0.2 324,994 + +0.0 324,994 + +0.2 324,994 + -0.5 324,994 + +1.0 324,995 - -0.1 324,995 - +0.3 324,995 - +0.1 324,995 - -1.4 324,995 - -0.6 324,995 - +1.2 324,995 - -2.4 324,995 - -0.3 324,998 + -0.7 324,998 + -0.8 324,998 + -0.3 324,998 + -1.7 324,999 - -1.0 324,999 - +0.5 324,999 - -1.4 324,999 - -0.8 324,999 - -0.4 325,031 + -1.7 325,031 + +0.4 325,031 + +0.2 325,032 - +0.9 325,080 + -1.1 325,081 - -0.5 325,081 - -1.0 325,120 + EX31_RS18720 0.13 -1.2 325,120 + EX31_RS18720 0.13 -1.0 325,121 - EX31_RS18720 0.13 -0.2 325,121 - EX31_RS18720 0.13 +1.0 325,162 + EX31_RS18720 0.17 +1.0 325,185 + EX31_RS18720 0.20 -0.1 325,375 + EX31_RS18720 0.42 +0.3 325,375 + EX31_RS18720 0.42 +0.6 325,375 + EX31_RS18720 0.42 -0.3 325,375 + EX31_RS18720 0.42 -0.8 325,376 - EX31_RS18720 0.42 +1.9 325,376 - EX31_RS18720 0.42 -2.1 325,381 + EX31_RS18720 0.43 +1.0 325,381 + EX31_RS18720 0.43 -1.0 325,566 + EX31_RS18720 0.65 +0.2 325,567 - EX31_RS18720 0.65 +0.3 325,567 - EX31_RS18720 0.65 -0.9 325,567 - EX31_RS18720 0.65 -0.9 325,567 - EX31_RS18720 0.65 +2.0 325,583 - EX31_RS18720 0.67 +0.4 325,611 - EX31_RS18720 0.70 +0.4 325,615 + EX31_RS18720 0.70 +1.2 325,615 + EX31_RS18720 0.70 -0.9 325,715 + EX31_RS18720 0.82 +1.3 325,715 + EX31_RS18720 0.82 -0.8 325,715 + EX31_RS18720 0.82 -0.2 325,716 - EX31_RS18720 0.82 -1.8 325,787 - +0.7 325,798 + -0.7 325,799 - +0.2 325,803 + -0.4 325,803 + +0.3 325,804 - +1.7 325,881 + +0.4 325,881 + +0.6 325,881 + -1.2 325,887 + -1.1 325,887 + -0.3 325,997 - +0.2
Or see this region's nucleotide sequence