Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18660 and EX31_RS18665 are separated by 158 nucleotides EX31_RS18665 and EX31_RS18670 are separated by 226 nucleotides
EX31_RS18660: EX31_RS18660 - cytidine deaminase, at 310,089 to 310,973
_RS18660
EX31_RS18665: EX31_RS18665 - NAD-dependent malic enzyme, at 311,132 to 312,829
_RS18665
EX31_RS18670: EX31_RS18670 - outer membrane permeability protein SanA, at 313,056 to 313,979
_RS18670
Position (kb) 311
312
313 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 310.210 kb on + strand, within EX31_RS18660 at 310.284 kb on - strand, within EX31_RS18660 at 310.284 kb on - strand, within EX31_RS18660 at 310.289 kb on + strand, within EX31_RS18660 at 310.290 kb on - strand, within EX31_RS18660 at 310.493 kb on + strand, within EX31_RS18660 at 310.494 kb on - strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.549 kb on + strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.550 kb on - strand, within EX31_RS18660 at 310.638 kb on + strand, within EX31_RS18660 at 310.707 kb on + strand, within EX31_RS18660 at 310.707 kb on + strand, within EX31_RS18660 at 310.707 kb on + strand, within EX31_RS18660 at 310.712 kb on + strand, within EX31_RS18660 at 310.713 kb on - strand, within EX31_RS18660 at 310.713 kb on - strand, within EX31_RS18660 at 310.835 kb on + strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.836 kb on - strand, within EX31_RS18660 at 310.952 kb on + strand at 310.975 kb on - strand at 310.975 kb on - strand at 310.994 kb on + strand at 310.994 kb on + strand at 310.995 kb on - strand at 311.019 kb on - strand at 311.022 kb on + strand at 311.022 kb on + strand at 311.023 kb on - strand at 311.023 kb on - strand at 311.026 kb on + strand at 311.027 kb on - strand at 311.027 kb on - strand at 311.039 kb on - strand at 311.060 kb on + strand at 311.060 kb on + strand at 311.060 kb on + strand at 311.061 kb on - strand at 311.095 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.165 kb on + strand at 311.166 kb on - strand at 311.166 kb on - strand at 311.228 kb on - strand at 311.357 kb on + strand, within EX31_RS18665 at 311.358 kb on - strand, within EX31_RS18665 at 311.358 kb on - strand, within EX31_RS18665 at 311.396 kb on + strand, within EX31_RS18665 at 311.442 kb on - strand, within EX31_RS18665 at 311.442 kb on - strand, within EX31_RS18665 at 311.486 kb on + strand, within EX31_RS18665 at 311.571 kb on + strand, within EX31_RS18665 at 311.571 kb on + strand, within EX31_RS18665 at 311.666 kb on + strand, within EX31_RS18665 at 311.666 kb on + strand, within EX31_RS18665 at 311.696 kb on + strand, within EX31_RS18665 at 311.696 kb on + strand, within EX31_RS18665 at 311.696 kb on + strand, within EX31_RS18665 at 311.697 kb on - strand, within EX31_RS18665 at 311.697 kb on - strand, within EX31_RS18665 at 311.698 kb on + strand, within EX31_RS18665 at 311.699 kb on - strand, within EX31_RS18665 at 311.742 kb on + strand, within EX31_RS18665 at 311.742 kb on + strand, within EX31_RS18665 at 311.743 kb on - strand, within EX31_RS18665 at 311.759 kb on + strand, within EX31_RS18665 at 311.759 kb on + strand, within EX31_RS18665 at 311.759 kb on + strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.760 kb on - strand, within EX31_RS18665 at 311.786 kb on - strand, within EX31_RS18665 at 311.971 kb on + strand, within EX31_RS18665 at 312.388 kb on + strand, within EX31_RS18665 at 312.388 kb on + strand, within EX31_RS18665 at 312.388 kb on + strand, within EX31_RS18665 at 312.497 kb on + strand, within EX31_RS18665 at 312.497 kb on + strand, within EX31_RS18665 at 312.497 kb on + strand, within EX31_RS18665 at 312.498 kb on - strand, within EX31_RS18665 at 312.498 kb on - strand, within EX31_RS18665 at 312.503 kb on - strand, within EX31_RS18665 at 312.688 kb on + strand at 312.688 kb on + strand at 312.809 kb on + strand at 312.810 kb on - strand at 312.810 kb on - strand at 312.811 kb on + strand at 312.811 kb on + strand at 312.811 kb on + strand at 312.811 kb on + strand at 312.841 kb on + strand at 312.911 kb on - strand at 312.925 kb on - strand at 312.988 kb on + strand at 312.988 kb on + strand at 312.989 kb on - strand at 312.989 kb on - strand at 313.008 kb on + strand at 313.008 kb on + strand at 313.014 kb on - strand at 313.042 kb on + strand at 313.051 kb on + strand at 313.051 kb on + strand at 313.051 kb on + strand at 313.055 kb on + strand at 313.055 kb on + strand at 313.055 kb on + strand at 313.055 kb on + strand at 313.056 kb on - strand at 313.056 kb on - strand at 313.061 kb on + strand at 313.061 kb on + strand at 313.061 kb on + strand at 313.070 kb on + strand at 313.071 kb on - strand at 313.072 kb on + strand at 313.072 kb on + strand at 313.072 kb on + strand at 313.072 kb on + strand at 313.072 kb on + strand at 313.073 kb on - strand at 313.073 kb on - strand at 313.073 kb on - strand at 313.073 kb on - strand at 313.073 kb on - strand at 313.094 kb on + strand at 313.116 kb on - strand at 313.116 kb on - strand at 313.135 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.141 kb on + strand at 313.142 kb on - strand at 313.142 kb on - strand at 313.142 kb on - strand at 313.142 kb on - strand at 313.142 kb on - strand at 313.161 kb on + strand, within EX31_RS18670 at 313.162 kb on - strand, within EX31_RS18670 at 313.172 kb on - strand, within EX31_RS18670 at 313.221 kb on + strand, within EX31_RS18670 at 313.221 kb on + strand, within EX31_RS18670 at 313.222 kb on - strand, within EX31_RS18670 at 313.222 kb on - strand, within EX31_RS18670 at 313.222 kb on - strand, within EX31_RS18670 at 313.222 kb on - strand, within EX31_RS18670 at 313.248 kb on + strand, within EX31_RS18670 at 313.248 kb on + strand, within EX31_RS18670 at 313.249 kb on - strand, within EX31_RS18670 at 313.251 kb on + strand, within EX31_RS18670 at 313.251 kb on + strand, within EX31_RS18670 at 313.251 kb on + strand, within EX31_RS18670 at 313.284 kb on + strand, within EX31_RS18670 at 313.284 kb on + strand, within EX31_RS18670 at 313.300 kb on + strand, within EX31_RS18670 at 313.305 kb on + strand, within EX31_RS18670 at 313.305 kb on + strand, within EX31_RS18670 at 313.306 kb on - strand, within EX31_RS18670 at 313.341 kb on + strand, within EX31_RS18670 at 313.342 kb on - strand, within EX31_RS18670 at 313.343 kb on + strand, within EX31_RS18670 at 313.344 kb on - strand, within EX31_RS18670 at 313.344 kb on - strand, within EX31_RS18670 at 313.485 kb on - strand, within EX31_RS18670 at 313.578 kb on - strand, within EX31_RS18670 at 313.636 kb on - strand, within EX31_RS18670 at 313.636 kb on - strand, within EX31_RS18670 at 313.636 kb on - strand, within EX31_RS18670 at 313.644 kb on - strand, within EX31_RS18670 at 313.679 kb on + strand, within EX31_RS18670 at 313.753 kb on + strand, within EX31_RS18670 at 313.753 kb on + strand, within EX31_RS18670 at 313.753 kb on + strand, within EX31_RS18670 at 313.754 kb on - strand, within EX31_RS18670
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 310,210 + EX31_RS18660 0.14 +0.7 310,284 - EX31_RS18660 0.22 -0.5 310,284 - EX31_RS18660 0.22 -0.6 310,289 + EX31_RS18660 0.23 +2.0 310,290 - EX31_RS18660 0.23 +1.4 310,493 + EX31_RS18660 0.46 +0.3 310,494 - EX31_RS18660 0.46 -1.1 310,549 + EX31_RS18660 0.52 -0.6 310,549 + EX31_RS18660 0.52 +0.1 310,549 + EX31_RS18660 0.52 -0.4 310,549 + EX31_RS18660 0.52 +0.4 310,549 + EX31_RS18660 0.52 +0.3 310,549 + EX31_RS18660 0.52 +3.0 310,549 + EX31_RS18660 0.52 -0.7 310,550 - EX31_RS18660 0.52 -0.4 310,550 - EX31_RS18660 0.52 -0.1 310,550 - EX31_RS18660 0.52 +1.2 310,550 - EX31_RS18660 0.52 -2.0 310,550 - EX31_RS18660 0.52 +0.4 310,550 - EX31_RS18660 0.52 -1.1 310,638 + EX31_RS18660 0.62 +1.3 310,707 + EX31_RS18660 0.70 +0.7 310,707 + EX31_RS18660 0.70 -0.3 310,707 + EX31_RS18660 0.70 +0.1 310,712 + EX31_RS18660 0.70 +0.6 310,713 - EX31_RS18660 0.71 +0.4 310,713 - EX31_RS18660 0.71 +1.2 310,835 + EX31_RS18660 0.84 -0.2 310,836 - EX31_RS18660 0.84 +0.7 310,836 - EX31_RS18660 0.84 -0.1 310,836 - EX31_RS18660 0.84 +2.0 310,836 - EX31_RS18660 0.84 +0.9 310,836 - EX31_RS18660 0.84 +1.2 310,952 + +0.4 310,975 - +0.1 310,975 - -2.0 310,994 + +0.4 310,994 + +1.1 310,995 - -1.2 311,019 - -0.8 311,022 + -1.1 311,022 + +0.6 311,023 - +1.0 311,023 - +0.0 311,026 + -0.8 311,027 - +2.0 311,027 - +1.3 311,039 - +0.8 311,060 + -0.1 311,060 + -1.3 311,060 + +0.8 311,061 - -1.1 311,095 + +1.2 311,165 + +0.0 311,165 + -0.2 311,165 + -0.1 311,165 + -0.0 311,165 + -1.9 311,166 - +0.8 311,166 - -0.1 311,228 - -1.3 311,357 + EX31_RS18665 0.13 +0.9 311,358 - EX31_RS18665 0.13 +0.2 311,358 - EX31_RS18665 0.13 +0.5 311,396 + EX31_RS18665 0.16 +0.5 311,442 - EX31_RS18665 0.18 +0.5 311,442 - EX31_RS18665 0.18 +0.4 311,486 + EX31_RS18665 0.21 -0.1 311,571 + EX31_RS18665 0.26 +0.1 311,571 + EX31_RS18665 0.26 +0.8 311,666 + EX31_RS18665 0.31 +1.0 311,666 + EX31_RS18665 0.31 -0.3 311,696 + EX31_RS18665 0.33 +1.3 311,696 + EX31_RS18665 0.33 +0.3 311,696 + EX31_RS18665 0.33 +0.3 311,697 - EX31_RS18665 0.33 -0.8 311,697 - EX31_RS18665 0.33 -0.8 311,698 + EX31_RS18665 0.33 +1.2 311,699 - EX31_RS18665 0.33 -0.1 311,742 + EX31_RS18665 0.36 +0.0 311,742 + EX31_RS18665 0.36 +0.1 311,743 - EX31_RS18665 0.36 +0.0 311,759 + EX31_RS18665 0.37 -0.4 311,759 + EX31_RS18665 0.37 +0.8 311,759 + EX31_RS18665 0.37 +0.1 311,760 - EX31_RS18665 0.37 -0.2 311,760 - EX31_RS18665 0.37 +0.7 311,760 - EX31_RS18665 0.37 -1.0 311,760 - EX31_RS18665 0.37 -1.1 311,760 - EX31_RS18665 0.37 +0.7 311,786 - EX31_RS18665 0.39 +0.4 311,971 + EX31_RS18665 0.49 -0.9 312,388 + EX31_RS18665 0.74 +0.7 312,388 + EX31_RS18665 0.74 +0.2 312,388 + EX31_RS18665 0.74 +0.4 312,497 + EX31_RS18665 0.80 -0.5 312,497 + EX31_RS18665 0.80 -0.2 312,497 + EX31_RS18665 0.80 +0.6 312,498 - EX31_RS18665 0.80 +2.2 312,498 - EX31_RS18665 0.80 +0.3 312,503 - EX31_RS18665 0.81 -1.9 312,688 + +0.7 312,688 + -0.4 312,809 + +1.7 312,810 - +0.0 312,810 - -1.1 312,811 + +0.3 312,811 + -2.0 312,811 + +0.2 312,811 + -0.4 312,841 + -0.4 312,911 - +1.2 312,925 - +2.2 312,988 + +2.0 312,988 + -0.1 312,989 - +0.0 312,989 - +2.0 313,008 + +0.6 313,008 + -0.8 313,014 - +0.4 313,042 + +1.1 313,051 + +0.2 313,051 + +0.0 313,051 + -1.1 313,055 + +1.1 313,055 + +0.0 313,055 + +0.8 313,055 + +0.2 313,056 - -0.1 313,056 - -1.2 313,061 + +0.2 313,061 + -0.1 313,061 + +0.9 313,070 + -0.4 313,071 - -0.3 313,072 + -3.3 313,072 + -0.1 313,072 + +0.3 313,072 + -0.3 313,072 + -0.0 313,073 - +0.3 313,073 - -0.3 313,073 - -1.9 313,073 - -1.7 313,073 - +1.4 313,094 + +0.9 313,116 - -1.4 313,116 - -0.9 313,135 + -0.7 313,141 + -1.0 313,141 + -0.0 313,141 + +0.3 313,141 + +2.4 313,141 + -0.1 313,141 + -0.2 313,141 + +0.8 313,141 + -0.3 313,142 - +0.9 313,142 - +1.2 313,142 - +0.4 313,142 - -0.0 313,142 - +1.2 313,161 + EX31_RS18670 0.11 +0.0 313,162 - EX31_RS18670 0.11 +0.9 313,172 - EX31_RS18670 0.13 -0.1 313,221 + EX31_RS18670 0.18 -1.6 313,221 + EX31_RS18670 0.18 +0.5 313,222 - EX31_RS18670 0.18 -0.2 313,222 - EX31_RS18670 0.18 -1.1 313,222 - EX31_RS18670 0.18 +0.0 313,222 - EX31_RS18670 0.18 +2.2 313,248 + EX31_RS18670 0.21 +0.7 313,248 + EX31_RS18670 0.21 -0.9 313,249 - EX31_RS18670 0.21 +2.6 313,251 + EX31_RS18670 0.21 -1.0 313,251 + EX31_RS18670 0.21 -0.0 313,251 + EX31_RS18670 0.21 -2.5 313,284 + EX31_RS18670 0.25 +0.5 313,284 + EX31_RS18670 0.25 +1.0 313,300 + EX31_RS18670 0.26 -1.1 313,305 + EX31_RS18670 0.27 -0.2 313,305 + EX31_RS18670 0.27 -1.7 313,306 - EX31_RS18670 0.27 -0.4 313,341 + EX31_RS18670 0.31 -0.5 313,342 - EX31_RS18670 0.31 +0.0 313,343 + EX31_RS18670 0.31 -0.8 313,344 - EX31_RS18670 0.31 -0.4 313,344 - EX31_RS18670 0.31 +0.4 313,485 - EX31_RS18670 0.46 +0.6 313,578 - EX31_RS18670 0.56 -0.1 313,636 - EX31_RS18670 0.63 -0.2 313,636 - EX31_RS18670 0.63 +1.6 313,636 - EX31_RS18670 0.63 +1.0 313,644 - EX31_RS18670 0.64 -0.2 313,679 + EX31_RS18670 0.67 -0.8 313,753 + EX31_RS18670 0.75 -1.0 313,753 + EX31_RS18670 0.75 +0.4 313,753 + EX31_RS18670 0.75 +1.7 313,754 - EX31_RS18670 0.76 +0.5
Or see this region's nucleotide sequence