Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16680 and EX31_RS16685 overlap by 4 nucleotides EX31_RS16685 and EX31_RS16690 are separated by 15 nucleotides EX31_RS16690 and EX31_RS16695 overlap by 4 nucleotides
EX31_RS16680: EX31_RS16680 - enoyl-CoA hydratase, at 18,742 to 19,512
_RS16680
EX31_RS16685: EX31_RS16685 - 3-hydroxyacyl-CoA dehydrogenase family protein, at 19,509 to 20,402
_RS16685
EX31_RS16690: EX31_RS16690 - CoA transferase, at 20,418 to 21,620
_RS16690
EX31_RS16695: EX31_RS16695 - aromatic amino acid lyase, at 21,617 to 23,161
_RS16695
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 19.778 kb on + strand, within EX31_RS16685 at 19.785 kb on + strand, within EX31_RS16685 at 19.858 kb on + strand, within EX31_RS16685 at 19.859 kb on - strand, within EX31_RS16685 at 19.859 kb on - strand, within EX31_RS16685 at 19.896 kb on - strand, within EX31_RS16685 at 19.956 kb on - strand, within EX31_RS16685 at 20.340 kb on + strand at 20.405 kb on + strand at 20.406 kb on - strand at 20.571 kb on - strand, within EX31_RS16690 at 20.955 kb on - strand, within EX31_RS16690 at 21.162 kb on - strand, within EX31_RS16690 at 21.193 kb on + strand, within EX31_RS16690 at 21.247 kb on + strand, within EX31_RS16690 at 21.247 kb on + strand, within EX31_RS16690 at 21.248 kb on - strand, within EX31_RS16690 at 21.248 kb on - strand, within EX31_RS16690 at 21.283 kb on + strand, within EX31_RS16690 at 21.434 kb on - strand, within EX31_RS16690 at 21.434 kb on - strand, within EX31_RS16690 at 21.434 kb on - strand, within EX31_RS16690 at 21.613 kb on - strand at 21.677 kb on - strand at 21.677 kb on - strand at 21.883 kb on + strand, within EX31_RS16695 at 21.884 kb on - strand, within EX31_RS16695 at 21.917 kb on - strand, within EX31_RS16695 at 21.949 kb on + strand, within EX31_RS16695 at 22.223 kb on - strand, within EX31_RS16695 at 22.223 kb on - strand, within EX31_RS16695 at 22.279 kb on - strand, within EX31_RS16695 at 22.279 kb on - strand, within EX31_RS16695 at 22.319 kb on - strand, within EX31_RS16695 at 22.360 kb on - strand, within EX31_RS16695 at 22.360 kb on - strand, within EX31_RS16695 at 22.364 kb on - strand, within EX31_RS16695 at 22.364 kb on - strand, within EX31_RS16695 at 22.573 kb on - strand, within EX31_RS16695
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 19,778 + EX31_RS16685 0.30 -0.0 19,785 + EX31_RS16685 0.31 +1.6 19,858 + EX31_RS16685 0.39 -1.9 19,859 - EX31_RS16685 0.39 -1.4 19,859 - EX31_RS16685 0.39 +1.6 19,896 - EX31_RS16685 0.43 +0.4 19,956 - EX31_RS16685 0.50 +1.2 20,340 + -2.1 20,405 + -0.3 20,406 - -1.2 20,571 - EX31_RS16690 0.13 -0.0 20,955 - EX31_RS16690 0.45 +0.1 21,162 - EX31_RS16690 0.62 -0.4 21,193 + EX31_RS16690 0.64 -1.2 21,247 + EX31_RS16690 0.69 +3.6 21,247 + EX31_RS16690 0.69 -1.6 21,248 - EX31_RS16690 0.69 -0.9 21,248 - EX31_RS16690 0.69 -0.4 21,283 + EX31_RS16690 0.72 +0.2 21,434 - EX31_RS16690 0.84 -0.7 21,434 - EX31_RS16690 0.84 -1.2 21,434 - EX31_RS16690 0.84 -0.4 21,613 - +0.6 21,677 - +0.6 21,677 - +0.8 21,883 + EX31_RS16695 0.17 +0.4 21,884 - EX31_RS16695 0.17 -1.6 21,917 - EX31_RS16695 0.19 +2.2 21,949 + EX31_RS16695 0.21 -0.7 22,223 - EX31_RS16695 0.39 -0.1 22,223 - EX31_RS16695 0.39 -1.9 22,279 - EX31_RS16695 0.43 +0.8 22,279 - EX31_RS16695 0.43 +0.3 22,319 - EX31_RS16695 0.45 +0.4 22,360 - EX31_RS16695 0.48 +0.5 22,360 - EX31_RS16695 0.48 +0.3 22,364 - EX31_RS16695 0.48 -0.0 22,364 - EX31_RS16695 0.48 -0.9 22,573 - EX31_RS16695 0.62 -0.3
Or see this region's nucleotide sequence